Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,430,396 A→G 11.8% intergenic (‑78/+83) PP_4769 ← / ← PP_4770 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,430,3960AG11.8% 127.5 / 8.9 51intergenic (‑78/+83)PP_4769/PP_4770hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (22/23);  new base G (2/4);  total (24/27)
Fisher's exact test for biased strand distribution p-value = 6.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.28e-01

CCGTGGGCCTGGCGCACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCAAAACCACCG  >  NC_002947/5430254‑5430536
                                                                                                                                              |                                                                                                                                             
ccGTGGGCCTGGCGCACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑cgcgc                                                                                                                                        <  2:160836/149‑1 (MQ=255)
ccGTGGGCCTGGCGCACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑cgcgc                                                                                                                                        >  6:3862/1‑149 (MQ=255)
  gggggCCTGGCGCACGGTTTAGTTGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGt                                                                                                                                      <  2:264383/147‑1 (MQ=255)
  gTGGGCCTGGCGCACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGt                                                                                                                                      <  6:294066/149‑1 (MQ=255)
            cgcACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTgg                                                                                                                            <  5:3862/149‑1 (MQ=255)
                cGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGc                                                                                                                        >  7:292180/1‑149 (MQ=255)
                 ggTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACGGGTGTACGCGCG‑ATACCTGTGGGAGc                                                                                                                        <  3:49291/148‑1 (MQ=37)
                 ggTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACGGGTGTACGCGCG‑ATACCTGTGGGAGc                                                                                                                        >  4:49291/1‑148 (MQ=37)
                  gtgtAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGGCACGCCCGCTCCCACGGGTGTACGCGCG‑ATACCTGTGGGAGCgg                                                                                                                      <  3:191410/149‑1 (MQ=37)
                       gtagAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTg                                                                                                                 >  7:254239/1‑149 (MQ=255)
                       gtagAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTg                                                                                                                 <  8:332418/149‑1 (MQ=255)
                       gtagAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACATGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTg                                                                                                                 >  3:278821/1‑149 (MQ=255)
                        tagAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTa                                                                                                                                     >  7:180826/1‑128 (MQ=255)
                        tagAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTa                                                                                                                                     <  8:180826/128‑1 (MQ=255)
                        tagAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGc                                                                                                                >  5:364836/1‑149 (MQ=255)
                          gagGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGccc                                                                                                              >  1:298454/1‑149 (MQ=255)
                          gagGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGC‑‑CCCTTCGCGGGCACGCCCGCTCCCACGGGTGTACGCGCG‑ATACCTGTGGGAGCGGGCGTGTCcg                                                                                                             <  3:361825/148‑1 (MQ=18)
                           agGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGccc                                                                                                              <  8:292180/148‑1 (MQ=255)
                            ggTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACGGGTGTACGCGCG‑ATACCTGTGGGAGCGGGCGTGTCcgc                                                                                                            >  4:119301/1‑149 (MQ=25)
                                   cacaTCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACGGGTGTACGCGCG‑ATACCTGTGGGAGCGGGCGTGTCCGCGAAg                                                                                                        <  3:119301/146‑1 (MQ=21)
                                   cacaTCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAgg                                                                                                       <  4:144710/147‑1 (MQ=255)
                                       tCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGgcgc                                                                                                  >  4:137391/1‑148 (MQ=255)
                                                          ttttCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCCCTCCCACAGGTAT‑CGCGCGTACACCCCTGGGAGCGGGCGTGCCCGCGAACGGGCGCAAACCGCCCCCCGAAATGc                                                                               >  8:303482/1‑148 (MQ=255)
                                                           tttCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCTCAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATg                                                                                <  2:258902/146‑1 (MQ=255)
                                                                    ggAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCTTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGc                                                                    <  5:263487/149‑1 (MQ=255)
                                                                    ggAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGc                                                                    >  4:249040/1‑149 (MQ=255)
                                                                       aCCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑cgcg                                                                                                                                         <  1:341258/77‑1 (MQ=255)
                                                                       aCCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑cgcg                                                                                                                                         >  2:341258/1‑77 (MQ=255)
                                                                        ccTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCCCTACCACAGGTAT‑CGCGCGTAAACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAACGCCCCCCGAAAAGCCAGCAGTATGCCCGa                                                                >  4:365607/1‑149 (MQ=38)
                                                                         cTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGAc                                                               <  2:298454/149‑1 (MQ=255)
                                                                         cTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGAc                                                               <  3:137391/149‑1 (MQ=255)
                                                                          tCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGAcc                                                              >  4:32878/1‑149 (MQ=255)
                                                                            aTTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCt                                                             <  3:365607/148‑1 (MQ=255)
                                                                                ttGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCGGTCAg                                                        >  8:149081/1‑149 (MQ=255)
                                                                                  ggCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGa                                                       <  8:254239/148‑1 (MQ=255)
                                                                                   gCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGAc                                                      <  7:303482/148‑1 (MQ=255)
                                                                                         aGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGaa                                               <  5:236249/149‑1 (MQ=255)
                                                                                              cGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAAtcgtc                                          <  3:32878/149‑1 (MQ=255)
                                                                                               ggggggCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑AGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGCCCTGTCAGACGTAAGAAtagtag                                         >  6:29432/1‑147 (MQ=255)
                                                                                                        ctgcGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGagaa                                 <  5:29432/148‑1 (MQ=255)
                                                                                                              aGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCa                          >  1:5676/1‑149 (MQ=255)
                                                                                                                  cTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTAGGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGt                      <  3:249040/149‑1 (MQ=255)
                                                                                                                              gCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCa           >  2:258364/1‑148 (MQ=255)
                                                                                                                               cccGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCaaa         <  6:364836/149‑1 (MQ=255)
                                                                                                                               cccGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCaa          >  3:45196/1‑148 (MQ=255)
                                                                                                                                ccGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCga                                    >  4:16550/1‑121 (MQ=255)
                                                                                                                                ccGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCga                                    <  3:16550/121‑1 (MQ=255)
                                                                                                                                ccGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCaaaa        >  3:101272/1‑149 (MQ=255)
                                                                                                                                      ccACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCAAAACCACCg  >  8:244663/1‑149 (MQ=255)
                                                                                                                                        acaGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCca                                                                             <  2:352608/72‑1 (MQ=255)
                                                                                                                                        acaGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCca                                                                             >  1:352608/1‑72 (MQ=255)
                                                                                                                                              |                                                                                                                                             
CCGTGGGCCTGGCGCACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCAAAACCACCG  >  NC_002947/5430254‑5430536

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑