Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,516,304	A→G	7.4%	I39V (ATC→GTC) ‡	PP_4849 →	molecular chaperone DnaK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,516,304	0	A	G	7.4%	232.8 / 7.0	81	I39V (ATC→GTC) ‡	PP_4849	molecular chaperone DnaK
Reads supporting (aligned to +/- strand):  ref base A (39/36);  new base G (2/4);  total (41/40)
Fisher's exact test for biased strand distribution p-value = 4.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.08e-01

GGCCGTTTTGCCTCCGGAAGCCCATTTCCCATGTCTGACGTATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGAAAAGCCTGCTGGGCTCGAAGCTGATCAAGCAC  >  NC_002947/5516160‑5516450                                                                                                                                                 |                                                                                                                                                   ggCCGTTTTGCCTCCGGAAGCCCATTTCCCATGTCTGACGTATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCAc                                                                                                                                                >  6:59078/1‑149 (MQ=255)         tGCCTCCGGAAGCCCATTTCCCATGTCTGACGTATCCCCGGCCCGCGCGCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGGTGATCTTGccg                                                                                                                                         <  1:140022/148‑2 (MQ=255)           ccTCCGGAAGCCCATTTCCCATGTCTGACGTATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGGTGATCTTGCCGTc                                                                                                                                       <  8:131073/148‑1 (MQ=255)           ccTCCGGAAGCCCATTTCCCATGTCTGACGTATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCg                                                                                                                                      <  5:37500/149‑1 (MQ=255)           ccTCCGGAAGCCCATTTCCCATGTCTGACGTATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCg                                                                                                                                      <  6:196435/149‑1 (MQ=255)            cTCCGGAAGCCCATTTCCCATGTCTGACGTATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCgg                                                                                                                                     >  5:238346/1‑149 (MQ=255)               cGGAAGCCCATTTCCCATGTCTGACGTATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGGTGATCTTGCCGTcttcca                                                                                                                                  <  2:28998/149‑6 (MQ=255)               cGGAAGCCCATTTCCCATGTCTGACGTATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCggtgg                                                                                                                                  <  1:136020/149‑1 (MQ=255)                ggAAGCCCATTTCCCATGTCTGACGTATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCggtggt                                                                                                                                 <  4:94036/149‑1 (MQ=255)                   aGCCCATTTCCCATGTCTGACGTATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGtt                                                                                                                              <  5:59078/149‑1 (MQ=255)                      ccATTTCCCATGTCTGACGTATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGttctt                                                                                                                           <  3:136104/149‑1 (MQ=255)                        aTTTCCCATGTCTGACGTATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCa                                                                                                                         <  5:186090/149‑1 (MQ=255)                           tCCCATGTGTGACGTAGCCCCGGCCCGCGCCCTGGCGATCGACTTTGTCACCTCCAACTCCAAAGTCGGTTGGCATCTGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACTTCAAGATCACCTTGCCCTCGGTTGTGTTCTTCAat                                                                                                                       <  8:106704/148‑1 (MQ=255)                            cccATGTCTGACGTATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAatat                                                                                                                     >  2:213460/1‑149 (MQ=255)                            cccATGTCTGACGTATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAata                                                                                                                      <  8:76311/148‑1 (MQ=255)                             ccATGTCTGACGTATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGttctt                                                                                                                           <  3:142995/142‑1 (MQ=255)                             ccATGTCTGACGTATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGttctt                                                                                                                           >  4:142995/1‑142 (MQ=255)                             ccATGTCTGACGTATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATc                                                                                                                    >  2:183921/1‑149 (MQ=255)                                      aCGTATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAagag                                                                                                              <  6:238346/146‑1 (MQ=255)                                             cccGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCgg                                                                                                    >  3:344351/1‑149 (MQ=255)                                              ccGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGgt                                                                                                   >  3:25319/1‑149 (MQ=255)                                              ccGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGgt                                                                                                   >  1:199664/1‑149 (MQ=255)                                               cGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGgtg                                                                                                  >  5:164371/1‑149 (MQ=255)                                                ggCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGttctt                                                                                                                           >  2:127991/1‑123 (MQ=255)                                                     gcgcCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCTATATCGAAGCGCGGCGCCCGGTCTATGgc                                                                                            >  4:219315/1‑149 (MQ=255)                                                             ggCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTg                                                                                      <  1:80462/147‑1 (MQ=255)                                                                aTCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCt                                                                                 >  8:188213/1‑149 (MQ=255)                                                                    aCTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCAc                                                                             <  4:25319/149‑1 (MQ=255)                                                                    aCTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCAc                                                                             <  2:199664/149‑1 (MQ=255)                                                                        tGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAg                                                                          <  3:219315/148‑1 (MQ=255)                                                                          gCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTAc                                                                       >  8:293339/1‑149 (MQ=255)                                                                          gCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTAc                                                                       >  8:229667/1‑149 (MQ=255)                                                                          gCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTAc                                                                       >  2:139907/1‑149 (MQ=255)                                                                           cACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTAcc                                                                      <  1:183921/149‑1 (MQ=255)                                                                               tCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTgg                                                                   >  6:278607/1‑148 (MQ=255)                                                                               tCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTgg                                                                   >  8:44111/1‑148 (MQ=255)                                                                                ccAACTCCACAGTCGGTTGGCATCGGCCTGGCCTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTACACGAGTACCTgg                                                                   >  1:44653/1‑147 (MQ=255)                                                                                  aaCTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAgg                                                               >  2:303738/1‑149 (MQ=255)                                                                                  aaCTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAgg                                                               >  2:299576/1‑149 (MQ=255)                                                                                     tCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTa                                                            >  4:38118/1‑149 (MQ=255)                                                                                       cacaGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACg                                                          <  3:38118/149‑1 (MQ=255)                                                                                         caGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACg                                                          >  8:217901/1‑147 (MQ=255)                                                                                              ggTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGgcc                                                    <  6:164371/148‑1 (MQ=255)                                                                                                ttGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGgccgcc                                                 <  4:344351/149‑1 (MQ=255)                                                                                                ttGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGgccgcc                                                 >  7:1287/1‑149 (MQ=255)                                                                                                  ggCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCt                                                <  7:293339/148‑1 (MQ=255)                                                                                                  ggCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCt                                                >  1:121936/1‑148 (MQ=255)                                                                                                         gCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACg                                                          >  5:194917/1‑131 (MQ=255)                                                                                                         gCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACg                                                          <  6:194917/131‑1 (MQ=255)                                                                                                         gCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGcgct                                        >  3:172272/1‑149 (MQ=255)                                                                                                              gCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGcgctcgc                                     <  5:163942/147‑1 (MQ=255)                                                                                                              gCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGcgctcgc                                     <  7:217901/147‑1 (MQ=255)                                                                                                                 tCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGaa                                 >  8:114643/1‑148 (MQ=255)                                                                                                                   gAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGaaaa                               <  7:188213/148‑1 (MQ=255)                                                                                                                     gTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGAAAAGcc                            <  5:278607/149‑1 (MQ=255)                                                                                                                      tCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGAAAAGCct                           <  1:139907/149‑1 (MQ=255)                                                                                                                        gCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGAAAAGCctg                          <  8:1287/148‑1 (MQ=255)                                                                                                                        gCTGATTGCCCTGGAAGACGGCAAGATCACCTGGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGAAAAGCctgc                         >  5:328250/1‑149 (MQ=255)                                                                                                                            aTTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTAc                                                                       >  1:194605/1‑99 (MQ=255)                                                                                                                            aTTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTAc                                                                       <  2:194605/99‑1 (MQ=255)                                                                                                                            aTTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGAAAAGCctgctg                       <  7:44111/147‑1 (MQ=255)                                                                                                                            aTTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGAAAAGCCTGCTggg                     >  5:232548/1‑149 (MQ=255)                                                                                                                                 ccTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGTAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGAAAAGCCTGCTGGGCTCg                 >  1:278437/1‑148 (MQ=255)                                                                                                                                   tGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGAAAAGCCTGCTGGGCTCGAAg              >  1:196673/1‑149 (MQ=255)                                                                                                                                   tGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGAAAAGCCTGCTGGGCTCGAAg              >  3:55313/1‑149 (MQ=255)                                                                                                                                     gAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGGGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGAAAAGCCTGCTGGGCTCGAAg              <  1:303738/147‑1 (MQ=255)                                                                                                                                     gAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGAAAAGCCTGCTGGGCTCGaa               >  4:46643/1‑146 (MQ=255)                                                                                                                                     gAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGAAAAGCCTGCTGGGCTCGaa               <  3:46643/146‑1 (MQ=255)                                                                                                                                     gAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGAAAAGCCTGCTGGGCTCGAAg              <  1:299576/147‑1 (MQ=255)                                                                                                                                     gAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGAAAAGCCTGCTGGGCTCGAAGc             <  2:278437/148‑1 (MQ=255)                                                                                                                                     gAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGAAAAGCCTGCTGGGCTCGAAGc             <  2:6729/148‑1 (MQ=255)                                                                                                                                     accgagggCAAGGTGATCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGAAAAGCCTGCTGGGCt                   <  7:59554/136‑1 (MQ=255)                                                                                                                                     accgagggCAAGGTGATCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGAAAAGCCTGCTGGGCt                   >  8:59554/7‑142 (MQ=255)                                                                                                                                      ccgagggCAAGGTGATCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGAAAAGCCTGCTGGGCTCGAAGCt            >  1:122688/6‑148 (MQ=255)                                                                                                                                       aGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTACACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGAAAAGCCTGCTGGGCTCGAAGCTGa          <  2:44653/149‑1 (MQ=255)                                                                                                                                           ggCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGAAAAGCCTGCTGGGCTCGAAg              <  6:13345/141‑1 (MQ=255)                                                                                                                                           ggCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGAAAAGCCTGCTGGGCTCGAAg              >  5:13345/1‑141 (MQ=255)                                                                                                                                           ggCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGAAAAGCCTGCTGGGCTCGAAGCTGATc        >  2:325432/1‑147 (MQ=255)                                                                                                                                             cAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGAAAAGCCTGCTGGGCTCGAAGCTGATCAAg     >  8:221299/1‑148 (MQ=255)                                                                                                                                                gATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGAAAAGCCTGCTGGGCTCGAAGCTGATCAAGCAc  >  1:266786/1‑148 (MQ=255)                                                                                                                                                gATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGAAAAGCCTGCTGGGCTCGAAGCTGATCAAGCAc  >  8:276830/1‑148 (MQ=255)                                                                                                                                                 |                                                                                                                                                   GGCCGTTTTGCCTCCGGAAGCCCATTTCCCATGTCTGACGTATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGAAAAGCCTGCTGGGCTCGAAGCTGATCAAGCAC  >  NC_002947/5516160‑5516450

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑