Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,516,308 C→T 7.3% T40I (ACC→ATC)  PP_4849 → molecular chaperone DnaK

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,516,3080CT 7.3% 243.5 / 5.3 81T40I (ACC→ATC) PP_4849molecular chaperone DnaK
Reads supporting (aligned to +/- strand):  ref base C (39/36);  new base T (2/4);  total (41/40)
Fisher's exact test for biased strand distribution p-value = 4.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.70e-01

GGCCGTTTTGCCTCCGGAAGCCCATTTCCCATGTCTGACGTATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGAAAAGCCTGCTGGGCTCGAAGCTGATCAAGCACGA  >  NC_002947/5516160‑5516452
                                                                                                                                                    |                                                                                                                                                
ggCCGTTTTGCCTCCGGAAGCCCATTTCCCATGTCTGACGTATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCAc                                                                                                                                                  >  6:59078/1‑149 (MQ=255)
        tGCCTCCGGAAGCCCATTTCCCATGTCTGACGTATCCCCGGCCCGCGCGCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGGTGATCTTGccg                                                                                                                                           <  1:140022/148‑2 (MQ=255)
          ccTCCGGAAGCCCATTTCCCATGTCTGACGTATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGGTGATCTTGCCGTc                                                                                                                                         <  8:131073/148‑1 (MQ=255)
          ccTCCGGAAGCCCATTTCCCATGTCTGACGTATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCg                                                                                                                                        <  5:37500/149‑1 (MQ=255)
          ccTCCGGAAGCCCATTTCCCATGTCTGACGTATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCg                                                                                                                                        <  6:196435/149‑1 (MQ=255)
           cTCCGGAAGCCCATTTCCCATGTCTGACGTATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCgg                                                                                                                                       >  5:238346/1‑149 (MQ=255)
              cGGAAGCCCATTTCCCATGTCTGACGTATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGGTGATCTTGCCGTcttcca                                                                                                                                    <  2:28998/149‑6 (MQ=255)
              cGGAAGCCCATTTCCCATGTCTGACGTATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCggtgg                                                                                                                                    <  1:136020/149‑1 (MQ=255)
               ggAAGCCCATTTCCCATGTCTGACGTATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCggtggt                                                                                                                                   <  4:94036/149‑1 (MQ=255)
                  aGCCCATTTCCCATGTCTGACGTATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGtt                                                                                                                                <  5:59078/149‑1 (MQ=255)
                     ccATTTCCCATGTCTGACGTATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGttctt                                                                                                                             <  3:136104/149‑1 (MQ=255)
                       aTTTCCCATGTCTGACGTATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCa                                                                                                                           <  5:186090/149‑1 (MQ=255)
                          tCCCATGTGTGACGTAGCCCCGGCCCGCGCCCTGGCGATCGACTTTGTCACCTCCAACTCCAAAGTCGGTTGGCATCTGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACTTCAAGATCACCTTGCCCTCGGTTGTGTTCTTCAat                                                                                                                         <  8:106704/148‑1 (MQ=255)
                           cccATGTCTGACGTATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAatat                                                                                                                       >  2:213460/1‑149 (MQ=255)
                           cccATGTCTGACGTATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAata                                                                                                                        <  8:76311/148‑1 (MQ=255)
                            ccATGTCTGACGTATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGttctt                                                                                                                             >  4:142995/1‑142 (MQ=255)
                            ccATGTCTGACGTATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGttctt                                                                                                                             <  3:142995/142‑1 (MQ=255)
                            ccATGTCTGACGTATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATc                                                                                                                      >  2:183921/1‑149 (MQ=255)
                                     aCGTATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAagag                                                                                                                <  6:238346/146‑1 (MQ=255)
                                            cccGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCgg                                                                                                      >  3:344351/1‑149 (MQ=255)
                                             ccGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGgt                                                                                                     >  1:199664/1‑149 (MQ=255)
                                             ccGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGgt                                                                                                     >  3:25319/1‑149 (MQ=255)
                                              cGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGgtg                                                                                                    >  5:164371/1‑149 (MQ=255)
                                               ggCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGttctt                                                                                                                             >  2:127991/1‑123 (MQ=255)
                                                    gcgcCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCTATATCGAAGCGCGGCGCCCGGTCTATGgc                                                                                              >  4:219315/1‑149 (MQ=255)
                                                            ggCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTg                                                                                        <  1:80462/147‑1 (MQ=255)
                                                               aTCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCt                                                                                   >  8:188213/1‑149 (MQ=255)
                                                                   aCTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCAc                                                                               <  4:25319/149‑1 (MQ=255)
                                                                   aCTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCAc                                                                               <  2:199664/149‑1 (MQ=255)
                                                                       tGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAg                                                                            <  3:219315/148‑1 (MQ=255)
                                                                         gCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTAc                                                                         >  8:229667/1‑149 (MQ=255)
                                                                         gCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTAc                                                                         >  8:293339/1‑149 (MQ=255)
                                                                         gCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTAc                                                                         >  2:139907/1‑149 (MQ=255)
                                                                          cACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTAcc                                                                        <  1:183921/149‑1 (MQ=255)
                                                                              tCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTgg                                                                     >  8:44111/1‑148 (MQ=255)
                                                                              tCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTgg                                                                     >  6:278607/1‑148 (MQ=255)
                                                                               ccAACTCCACAGTCGGTTGGCATCGGCCTGGCCTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTACACGAGTACCTgg                                                                     >  1:44653/1‑147 (MQ=255)
                                                                                 aaCTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAgg                                                                 >  2:303738/1‑149 (MQ=255)
                                                                                 aaCTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAgg                                                                 >  2:299576/1‑149 (MQ=255)
                                                                                    tCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTa                                                              >  4:38118/1‑149 (MQ=255)
                                                                                      cacaGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACg                                                            <  3:38118/149‑1 (MQ=255)
                                                                                        caGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACg                                                            >  8:217901/1‑147 (MQ=255)
                                                                                             ggTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGgcc                                                      <  6:164371/148‑1 (MQ=255)
                                                                                               ttGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGgccgcc                                                   >  7:1287/1‑149 (MQ=255)
                                                                                               ttGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGgccgcc                                                   <  4:344351/149‑1 (MQ=255)
                                                                                                 ggCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCt                                                  >  1:121936/1‑148 (MQ=255)
                                                                                                 ggCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCt                                                  <  7:293339/148‑1 (MQ=255)
                                                                                                        gCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACg                                                            >  5:194917/1‑131 (MQ=255)
                                                                                                        gCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACg                                                            <  6:194917/131‑1 (MQ=255)
                                                                                                        gCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGcgct                                          >  3:172272/1‑149 (MQ=255)
                                                                                                             gCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGcgctcgc                                       <  7:217901/147‑1 (MQ=255)
                                                                                                             gCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGcgctcgc                                       <  5:163942/147‑1 (MQ=255)
                                                                                                                tCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGaa                                   >  8:114643/1‑148 (MQ=255)
                                                                                                                  gAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGaaaa                                 <  7:188213/148‑1 (MQ=255)
                                                                                                                    gTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGAAAAGcc                              <  5:278607/149‑1 (MQ=255)
                                                                                                                     tCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGAAAAGCct                             <  1:139907/149‑1 (MQ=255)
                                                                                                                       gCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGAAAAGCctg                            <  8:1287/148‑1 (MQ=255)
                                                                                                                       gCTGATTGCCCTGGAAGACGGCAAGATCACCTGGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGAAAAGCctgc                           >  5:328250/1‑149 (MQ=255)
                                                                                                                           aTTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTAc                                                                         <  2:194605/99‑1 (MQ=255)
                                                                                                                           aTTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTAc                                                                         >  1:194605/1‑99 (MQ=255)
                                                                                                                           aTTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGAAAAGCctgctg                         <  7:44111/147‑1 (MQ=255)
                                                                                                                           aTTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGAAAAGCCTGCTggg                       >  5:232548/1‑149 (MQ=255)
                                                                                                                                ccTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGTAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGAAAAGCCTGCTGGGCTCg                   >  1:278437/1‑148 (MQ=255)
                                                                                                                                  tGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGAAAAGCCTGCTGGGCTCGAAg                >  1:196673/1‑149 (MQ=255)
                                                                                                                                  tGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGAAAAGCCTGCTGGGCTCGAAg                >  3:55313/1‑149 (MQ=255)
                                                                                                                                    gAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGGGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGAAAAGCCTGCTGGGCTCGAAg                <  1:303738/147‑1 (MQ=255)
                                                                                                                                    gAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGAAAAGCCTGCTGGGCTCGaa                 <  3:46643/146‑1 (MQ=255)
                                                                                                                                    gAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGAAAAGCCTGCTGGGCTCGaa                 >  4:46643/1‑146 (MQ=255)
                                                                                                                                    gAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGAAAAGCCTGCTGGGCTCGAAg                <  1:299576/147‑1 (MQ=255)
                                                                                                                                    gAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGAAAAGCCTGCTGGGCTCGAAGc               <  2:278437/148‑1 (MQ=255)
                                                                                                                                    gAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGAAAAGCCTGCTGGGCTCGAAGc               <  2:6729/148‑1 (MQ=255)
                                                                                                                                    accgagggCAAGGTGATCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGAAAAGCCTGCTGGGCt                     >  8:59554/7‑142 (MQ=255)
                                                                                                                                    accgagggCAAGGTGATCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGAAAAGCCTGCTGGGCt                     <  7:59554/136‑1 (MQ=255)
                                                                                                                                     ccgagggCAAGGTGATCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGAAAAGCCTGCTGGGCTCGAAGCt              >  1:122688/6‑148 (MQ=255)
                                                                                                                                      aGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTACACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGAAAAGCCTGCTGGGCTCGAAGCTGa            <  2:44653/149‑1 (MQ=255)
                                                                                                                                          ggCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGAAAAGCCTGCTGGGCTCGAAg                <  6:13345/141‑1 (MQ=255)
                                                                                                                                          ggCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGAAAAGCCTGCTGGGCTCGAAg                >  5:13345/1‑141 (MQ=255)
                                                                                                                                          ggCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGAAAAGCCTGCTGGGCTCGAAGCTGATc          >  2:325432/1‑147 (MQ=255)
                                                                                                                                            cAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGAAAAGCCTGCTGGGCTCGAAGCTGATCAAg       >  8:221299/1‑148 (MQ=255)
                                                                                                                                               gATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGAAAAGCCTGCTGGGCTCGAAGCTGATCAAGCAc    >  1:266786/1‑148 (MQ=255)
                                                                                                                                               gATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGAAAAGCCTGCTGGGCTCGAAGCTGATCAAGCAc    >  8:276830/1‑148 (MQ=255)
                                                                                                                                                 tCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGAAAAGCCTGCTGGGCTCGAAGCTGATCAAGCACGa  >  6:297238/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
GGCCGTTTTGCCTCCGGAAGCCCATTTCCCATGTCTGACGTATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGAAAAGCCTGCTGGGCTCGAAGCTGATCAAGCACGA  >  NC_002947/5516160‑5516452

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑