Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,591,504 C→T 5.7% P126P (CCG→CCA)  nudF ← ADP‑ribose/sugar pyrophosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,591,5040CT 5.7% 214.5 / 2.1 70P126P (CCG→CCA) nudFADP‑ribose/sugar pyrophosphatase
Reads supporting (aligned to +/- strand):  ref base C (36/30);  new base T (2/2);  total (38/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.29e-01

ACGCCCACACGCGTACGCGAATGTCTTCGCCCTCTTCCTCCAGGCCATGCAGCCCTCCCGCGCCCTCGCTGCTGCAACGGCCGAGGAACAGATGAACGTATTCGTCACTGCCGCCAGGCGACGGGAAGTAGCGGGTCATCGGCCACAGGGCGCTGAAGGTCAGGCCGGCTTCTTCTTCAGCCTCGCGATGGGCGACTTCCTCGGGCTGCTCGTCTTTGTCGATCAGCCCGGCAACCATCTCGATCAGCCAGGGGTTGGCGACCTTGTCCAGGGCGCCCACGCGGAAC  >  NC_002947/5591365‑5591651
                                                                                                                                           |                                                                                                                                                   
aCGCCCACACGCGTACGCGAATGTCTTCGCCCTCTTCCTCCAGGCCATGCAGCCCTCCCGCGCCCTCGCTGCTGCAACGGCCGAGGAACAGATGAACGTATTCGTCACTGCCGCCAGGCGACGGGAAGTAGCGGGTCATCGGCCACAgg                                                                                                                                            >  7:6299/1‑149 (MQ=255)
  gCCCACACGCGTACGCGAATGTCTTCGCCCTCTTCCTCCAGGCCATGCAGCCCTCCCGCGCCCTCGCTGCTGCAACGGCCGAGGAACAGATGAACGTATTCGTCACTGCCGCCAGGCGACGGGAAGTAGCGGGTCATCGGCCACAGGgc                                                                                                                                          >  3:297832/1‑149 (MQ=255)
             tACGCGAATGTCTTCGCCCTCTTCCTCCAGGCCATGCAGCCCTCCCGCGCCCTCGCTGCTGCAACGGCCGAGGAACAGATGAACGTATTCGTCACTGCCGCCAGGCGACGGGAAGTAGCGGGTCATCGGCCACAGGGCGCTGAAGGt                                                                                                                                 >  2:174096/1‑147 (MQ=255)
                  gAATGTCTTCGCCCTCTTCCTCCAGGCCATGCAGCCCTCCCGCGCCCTCGCTGCTGCAACGGCCGAGGAACAGATGAACGTATTCGTCACTGCCGCCAGGCGACGGGAAGTAGCGGGTCATCGGCCACAGGGCGCTGAAGGTCAGGCCg                                                                                                                          >  3:90739/1‑149 (MQ=255)
                  gAATGTCTTCGCCCTCTTCCTCCAGGCCATGCAGCCCTCCCGCGCCCTCGCTGCTGCAACGGCCGAGGAACAGATGAACGTATTCGTCACTGCCGCCAGGCGACGGGAAGTAGCGGGTCATCGGCCACAGGGCGCTGAAGGTCAGGCCg                                                                                                                          >  5:337381/1‑149 (MQ=255)
                         ttCGCCCTCTTCCTCCAGGCCATGCAGCCCTCCCGCGCCCTCGCTGCTGCAACGGCCGAGGAACAGATGAACGTATTCGTCACTGCCGCCAGGCGACGGGAAGTAGCGGGTCATCGGCCACAgg                                                                                                                                            >  4:269169/1‑124 (MQ=255)
                         ttCGCCCTCTTCCTCCAGGCCATGCAGCCCTCCCGCGCCCTCGCTGCTGCAACGGCCGAGGAACAGATGAACGTATTCGTCACTGCCGCCAGGCGACGGGAAGTAGCGGGTCATCGGCCACAgg                                                                                                                                            <  3:269169/124‑1 (MQ=255)
                            gCCCTCTTCCTCCAGGCCATGCAGCCCTCCCGCGCCCTCGCTGCTGCAACGGCCGAGGAACAGATGAACGTATTCGTCACTGCCGCCAGGCGACGGGAAGTAGCGGGTCATCGGCCACAGGGCGCTGAAGGTCAGGCCGGcttcttctt                                                                                                                <  1:126980/149‑1 (MQ=255)
                             cccTCTTCCTCCAGGCCATGCAGCCCTCCCGCGCCCTCGCTGCTGCAACGGCCGAGGAACAGATGAACGTATTCGTCACTGCCGCCAGGCGACGGGAAGTAGCGGGTCATCGGCCACAGGGCGCTGAAGGTCAGGCCGGcttcttctt                                                                                                                >  6:365174/1‑148 (MQ=255)
                             cccTCTTCCTCCAGGCCATGCAGCCCTCCCGCGCCCTCGCTGCTGCAACGGCCGAGGAACAGATGAACGTATTCGTCACTGCCGCCAGGCGACGGGAAGTAGCGGGTCATCGGCCACAGGGCGCTGAAGGTCAGGCCGGCttcttcttc                                                                                                               <  8:6299/149‑1 (MQ=255)
                              ccTCTTCCTCCAGGCCATGCAGCCCTCCCGCGCCCTCGCTGCTGCAACGGCCGAGGAACAGATGAACGTATTCGTCACTGCCGCCAGGCGACGGGAAGTAGCGGGTCATCGGCCACAGGGCGCTGAAGGTCAGGCCGGcttcttctt                                                                                                                >  8:48426/1‑147 (MQ=255)
                               ctctTCCTCCAGGCCATGCAGCCCTCCCGCGCCCTCGCTGCTGCAACGGCCGAGGAACAGATGAACGTATTCGTCACTGCCGCCAGGCGACGGGAAGTAGCGGGTCATCGGCCACAGGGCGCTGAAGGTCAGGc                                                                                                                            >  4:280891/1‑134 (MQ=255)
                               ctctTCCTCCAGGCCATGCAGCCCTCCCGCGCCCTCGCTGCTGCAACGGCCGAGGAACAGATGAACGTATTCGTCACTGCCGCCAGGCGACGGGAAGTAGCGGGTCATCGGCCACAGGGCGCTGAAGGTCAGGc                                                                                                                            <  3:280891/134‑1 (MQ=255)
                               ctctTCCTCCAGGCCATGCAGCCCTCCCGCGCCCTCGCTGCTGCAACGGCCGAGGAACAGATGAACGTATTCGTCACTGCCGCCAGGCGACGGGAAGTAGCGGGTCATCGGCCACAGGGCGCTGAAGGTCAGGCCGGCTTCTTCTTCAg                                                                                                             >  5:337757/1‑149 (MQ=255)
                                tctTCCTCCAGGCCATGCAGCCCTCCCGCGCCCTCGCTGCTGCAACGGCCGAGGAACAGATGAACGTATTCGTCACTGCCGCCAGGCGACGGGAAGTAGCGGGTCATCGGCCACAGGGCGCTGAAGGTCAGGCCGGCTTCTTCTTCAGc                                                                                                            >  2:162747/1‑149 (MQ=255)
                                     ctccAGGCCATGCAGCCCTCCCGCGCCCTCGCTGCTGCAACGGCCGAGGAACAGATGAACGTATTCGTCACTGCCGCCAGGCGACGGGAAGTAGCGGGTCATCGGGCACAGGGCGCTGAAGGTCAGGCCGGCTTCTTCTTCAGCCTcgc                                                                                                       >  3:176122/1‑149 (MQ=255)
                                      tccAGGCCATGCAGCCCTCCCGCGCCCTCGCTGCTGCAACGGCCGAGGAACAGATGAACGTATTCGTCACTGCCGCCAGGCGACGGGAAGTAGCGGGTCATCGGCCACAGGGCGCTGAAGGTCAGGCCGGCTTCTTCTTCAGCCTcgc                                                                                                       >  8:141222/1‑148 (MQ=255)
                                      tccAGGCCATGCAGCCCTCCCGCGCCCTCGCTGCTGCAACGGCCGAGGAACAGATGAACGTATTCGTCACTGCCGCCAGGCGACGGGAAGTAGCGGGTCATCGGCCACAGGGCGCTGAAGGTCAGGCCGGCTTCTTCTTCAGCCTcgc                                                                                                       >  5:94394/1‑148 (MQ=255)
                                           gCCATGCAGCCCTCCCGCGCCCTCGCTGCTGCAACGGCCGAGGAACAGATGAACGTATTCGTCACTGCCGCCAGGCGACGGGAAGTAGCGGGTCATCGGCCACAGGGCGCTGAAGGTCAGGCCGGCTTCTTCTTCAGCCTCGCGATggg                                                                                                 >  6:150788/1‑149 (MQ=255)
                                           gCCATGCAGCCCTCCCGCGCCCTCGCTGCTGCAACGGCCGAGGAACAGATGAACGTATTCCTCACTGCCGCCAGGCGACGGGAAGTAGCGGGTCATCGGCCACAGGGCGCTGAAGGTCAGGCCGGCTTCTTCTTCAGCCTCGCGATggg                                                                                                 >  6:330659/1‑149 (MQ=255)
                                                 cAGCCCTCCCGCGCCCTCGCTGCTGCAACGGCCGAGGAACAGATGAACGTATTCGTCACTGCCGCCAGGCGACGGGAAGTAGCGGGTCATCGGCCACAGGGCGCTGAAGGTCAGGCCGGCTTCTTCTTCAGCCTCGCGATGGGCGACt                                                                                            <  1:174096/148‑1 (MQ=255)
                                                    cccTCCCGCGCCCTCGCTGCTGCAACGGCCGAGGAACAGATGAACGTATTCGTCACTGCCGCCAGGCGACGGGAAGTAGCGGGTCATTGGCCACAGGGCGCTGAAGGTCAGGCCGGCTTCTTCTTCAGCCTCGCGATGGGCGACTTCCt                                                                                        >  6:346978/1‑149 (MQ=255)
                                                    cccTCCCGCGCCCTCGCTGCTGCAACGGCCGAGGAACAGATGAACGTATTCGTCACTGCCGCCAGGCGACGGGAAGTAGCGGGTCATTGGCCACAGGGCGCTGAAGGTCAGGCCGGCTTCTTCTTCAGCCTCGCGATGGGCGACTTCCt                                                                                        >  6:341528/1‑149 (MQ=255)
                                                        cccGCGCCCTCGCTGCTGCAACGGCCGAGGAACAGATGAACGTATTCGTCACTGCCGCCAGGCGACGGGAAGTAGCGGGTCATCGGCCACAGGGCGCTGAAGGTCAGGCCGGCTTCTTCTTCAGCCTCGCGATGGGCGACTTCCTCggg                                                                                    >  2:97640/1‑149 (MQ=255)
                                                         ccGCGCCCTCGCTGCTGCAACGGCCGAGGAACAGATGAACGTATTCGTCACTGCCGCCAGGCGACGGGAAGTAGCGGGTCATCGGCCACAGGGCGCTGAAGGTCAGGCCGGCTTCTTCTTCAGCCTCGCGATGGGCGACTTCCTCGGgc                                                                                   >  3:89947/1‑149 (MQ=255)
                                                               ccTCGCTGCTGCAACGGCCGAGGAACAGATGAACGTATTCGTCACTGCCGCCAGGCGACGGGAAGTAGCGGGTCATCGGCCACAGGGCGCTGAAGGTCAGGCCGGCTTCTTCTTCAGCCTCGCGATGGGCGACTTCCTCGGGCTGCTCg                                                                             >  7:124822/1‑149 (MQ=255)
                                                                cTCGCTGCTGCAACGGCCGAGGAACAGATGAACGTATTCGTCACTGCCGCCAGGCGACGGGAAGTAGCGGGTCATCGGCCACAGGGCGCTGAAGGTCAGGCCGGCTTCTTCTTCAGCCTCGCGAt                                                                                                    <  6:266502/125‑1 (MQ=255)
                                                                cTCGCTGCTGCAACGGCCGAGGAACAGATGAACGTATTCGTCACTGCCGCCAGGCGACGGGAAGTAGCGGGTCATCGGCCACAGGGCGCTGAAGGTCAGGCCGGCTTCTTCTTCAGCCTCGCGAt                                                                                                    >  5:266502/1‑125 (MQ=255)
                                                                   gctgctGCAACGGCCGAGGAACAGATGAACGTATTCGTCACTGCCGCCAGGCGACGGGAAGTAGCGGGTCATCGGCCACAGGGCGCTGAAGGTCAGGCCGGCTTCTTCTTCAGCCTCGCGATGGGCGACTTCCTCGGGCTGCTCGTc                                                                           <  6:337381/147‑1 (MQ=255)
                                                                   gctgctGCAACGGCCGAGGAACAGATGAACGTATTCGTCACTGCCGCCAGGCGACGGGAAGTAGCGGGTCATCGGCCACAGGGCGCTGAAGGTCAGGCCGGCTTCTTCTTCAGCCTCGCGATGGGCGACTTCCTCGGGCTGCTCGTCt                                                                          <  8:124822/148‑1 (MQ=255)
                                                                       ctgcAACGGCCGAGGAACAGATGAACGTATTCGTCACTGCCGCCAGGCGACGGGAAGTAGCGGGTCATCGGCCACAGGGCGCTGAAGGTCAGGCCGGCTTCTTCTTCAGCCTCGCGATGGGCGACTTCCTCGGGCTGCTCGTCTTTGt                                                                      <  4:297832/148‑1 (MQ=255)
                                                                       ctgcAACGGCCGAGGAACAGATGAACGTATTCGTCACTGCCGCCAGGCGACGGGAAGTAGCGGGTCATCGGCCACAGGGCGCTGAAGGTCAGGCCGGCTTCTTCTTCAGCCTCGCGATGGGCGACTTCCTCGGGCTGCTCGTCTTTGt                                                                      <  1:97640/148‑1 (MQ=255)
                                                                         gcAACGGCCGAGGAACAGATGAACGTATTCGTCACTGCCGCCAGGCGACGGGAAGTAGCGGGTCATCGGCCACAGGGCGCTGAAGGTCAGGCCGGCTTCTTCTTCAGCCTCGCGATTGGCGACTTCCTCGGGCTGCTCGTCTTTGt                                                                      <  2:317429/146‑1 (MQ=255)
                                                                         gcAACGGCCGAGGAACAGATGAACGTATTCGTCACTGCCGCCAGGCGACGGGAAGTAGCGGGTCATCGGCCACAGGGCGCTGAAGGTCAGGCCGGCTTCTTCTTCAGCCTCGCGATGGGCGACTTCCTCGGGCTGCTCGTCTTTGt                                                                      >  1:317429/1‑146 (MQ=255)
                                                                         gcAACGGCCGAGGAACAGATGAACGTATTCGTCACTGCCGCCAGGCGACGGGAAGTAGCGGGTCATCGGCCACAGGGCGCTGAAGGTCAGGCCGGCTTCTTCTTCAGCCTCGCGATGGGCGACTTCCTCGGGCTGCTCGTCTTTGTCGa                                                                   >  4:185585/1‑149 (MQ=255)
                                                                              ggCCGAGGAACAGATGAACGTATTCGTCACTGCCGCCAGGCGACGGGAAGTAGCGGGTCATCGGCCACAGGGCGCTGAAGGTCAGGCCGGCTTCTTCTTCAGCCTCGCGATGGGCGACTTCCTCGGGCTGCTCGTCTTTGTCGATCAGc                                                              >  6:353207/1‑149 (MQ=255)
                                                                                      aaCAGATGAACGTATTCGTCACTGCCGCCAGGCGACGGGAAGTAGCGGGTCATCGGCCACAGGGCGCTGAAGGTCAGGCCGGCTTCTTCTTCa                                                                                                              >  8:290860/1‑93 (MQ=255)
                                                                                      aaCAGATGAACGTATTCGTCACTGCCGCCAGGCGACGGGAAGTAGCGGGTCATCGGCCACAGGGCGCTGAAGGTCAGGCCGGCTTCTTCTTCa                                                                                                              <  7:290860/93‑1 (MQ=255)
                                                                                       aCAGATGAACGTATTCGTCACTGCCGCCAGGCGACGGGAAGTAGCGGGTCATCGGCCACAGGGCGCTGAAGGTCAGGCCGGCTTCTTCTTCAGCCTCGCGATGGGCGACTTCCTCg                                                                                      <  4:65162/116‑1 (MQ=255)
                                                                                       aCAGATGAACGTATTCGTCACTGCCGCCAGGCGACGGGAAGTAGCGGGTCATCGGCCACAGGGCGCTGAAGGTCAGGCCGGCTTCTTCTTCAGCCTCGCGATGGGCGACTTCCTCg                                                                                      >  3:65162/1‑116 (MQ=255)
                                                                                        cAGATGAACGTATTCGTCACTGCCGCCAGGCGACGGGAAGTAGCGGGTCATCGGCCACAGGGCGCTGAAGGTCAGGCCGGCTTCTTCTTCAGCCTCGCGATGGGCGACTTCCTCGGGCTGCTCGTCTTTGTCGATCAGCCCGGCAACCa                                                    <  8:349185/149‑1 (MQ=255)
                                                                                           aTGAACGTATTCGTCACTGCCGCCAGGCGACGGGAAGTAGCGGGTCATTGGCCACAGGGCGCTGAAGGTCAGGCCGGCTTCTTCTTCAGCCTCGCGATGGGCGACTTCCTCGGGCTGCTCGTCTTTGTCGATCAGCCCGGCAACCAtct                                                 <  5:341528/149‑1 (MQ=255)
                                                                                           aTGAACGTATTCGTCACTGCCGCCAGGCGACGGGAAGTAGCGGGTCATTGGCCACAGGGCGCTGAAGGTCAGGCCGGCTTCTTCTTCAGCCTCGCGATGGGCGACTTCCTCGGGCTGCTCGTCTTTGTCGATCAGCCCGGCAACCAtct                                                 <  5:346978/149‑1 (MQ=255)
                                                                                              aaCGTATTCGTCACTGCCGCCAGGCGACGGGAAGTAGCGGGTCATCGGCCACAGGGCGCTGAAGGTCAGGCCGGCTTCTTCTTCAGCCTCGCGATGGGCGACTTCCTCGGGCTGCTCGTCTTTGTCGATCAGCCCGGCAACCATCTCg                                               <  3:185585/148‑1 (MQ=255)
                                                                                              aaCGTATTCGTCACTGCCGCCAGGCGACGGGAAGTAGCGGGTCATCGGCCACAGGGCGCTGAAGGTCAGGCCGGCTTCTTCTTCAGCCTCGCGATGGGCGACTTCCTCGGGCTGCTCGTCTTTGTCGATCAGCCCGGCAACCATCTCg                                               <  6:337757/148‑1 (MQ=255)
                                                                                              aaCGTATTCGTCACTGCCGCCAGGCGACGGGAAGTAGCGGGTCATCGGCCACAGGGCGCTGAAGGTCAGGCCGGCTTCTTCTTCAGCCTCGCGATGGGCGACTTCCTCGGGCTGCTCGTCTTTGTCGATCAGCCCGGCAACCATCTCGa                                              <  5:150788/149‑1 (MQ=255)
                                                                                                 gTATTCGTCACTGCCGCCAGGCGACGGGAAGTAGCGGGTCATCGGCCACAGGGCGCTGAAGGTCa                                                                                                                               <  4:254508/65‑1 (MQ=255)
                                                                                                   aTTCGTCACTGCCGCCAGGCGACGGGAAGTAGCGGGTCATCGGCCACAGGGCGCTGAAGGTCAGGCCGGCTTCTTCTTCAGCCTCGCGATGGGCGACTTCCTCGGGCTGCTCGTCTTTGTCGATCAGCCCGGCAACCATCTCGATCAGc                                         >  3:40876/1‑149 (MQ=255)
                                                                                                    ttCGTCACTGCCGCCAGGCGACGGGAAGTAGCGGGTCATCGGCCACAGGGCGCTGAAGGTCa                                                                                                                               <  5:295108/62‑1 (MQ=255)
                                                                                                    ttCGTCACTGCCGCCAGGCGACGGGAAGTAGCGGGTCATCGGCCACAGGGCGCTGAAGGTCa                                                                                                                               >  6:295108/1‑62 (MQ=255)
                                                                                                        tCACTGCCGCCAGGCGACGGGAAGTAGCGGGTCATCGGCCACAGGGCGCTGAAGGTCAGGCCGGCTTCTTCTTCAGCCTCGCGATGGGCGACTTCCTCGGGCTGCTCGTCTTTGTCGATCAGCCCGGCAACCATCTCGATCAGCCAggg                                    >  7:161387/1‑149 (MQ=255)
                                                                                                        tCACTGCCGCCAGGCGACGGGAAGTAGCGGGTCATCGGCCACAGGGCGCTGAAGGTCAGGCCGGCTTCTTCTTCAGCCTCGCGATGGGCGACTTCCTCGGGCTGCTCGTCTTTGTCGATCAGCCCGGCAACCATCTCGATCAGCCAggg                                    <  7:141222/149‑1 (MQ=255)
                                                                                                          aCTGCCGCCAGGCGACGGGAAGTAGCGGGTCATCGGGCACAGGGCGCTGAAGGTCAGGCCGGCTTCTTCTTCAGCCTCGCGATGGGCGACTTCCTCGGGCTGCTCGTCTTTGTCGATCAGCCCGGCAACCATCTCGATCAGCCAGGGGt                                  <  4:176122/149‑1 (MQ=255)
                                                                                                           cTGCCGCCAGGCGACGGGAAGTAGCGGGTCATCGGCCACAGGGCGCTGAAGGTCAGGCCGGCTTCTTCTTCAGCCTCGCGATGGGCGACTTCCTCGGGCTGCTCGTCTTTGTCGATCAGCCCGGCAACCATCTCGATCAGCCAGGGGtt                                 >  1:47725/1‑149 (MQ=255)
                                                                                                              ccgccAGGCGACGGGAAGTAGCGGGTCATCGGCCACAGGGCGCTGAAGGTCAGGCCGGCTTCTTCTTCAGCCTCGCGATGGGCGACTTCCTCGGGCTGCTCGTCTTTGTCGATCAGCCCGGCAACCATCTCGATCAGCCAGGGGTTGGc                              <  4:89947/149‑1 (MQ=255)
                                                                                                                 ccAGGCGACGGGAAGTAGCGGGTCATCGGCCACAGGGCGCTGAAGGTCAGGCCGGCTTCTTCTTCAGCCTCGCGATGGGCGAc                                                                                             >  8:349931/1‑83 (MQ=255)
                                                                                                                 ccAGGCGACGGGAAGTAGCGGGTCATCGGCCACAGGGCGCTGAAGGTCAGGCCGGCTTCTTCTTCAGCCTCGCGATGGGCGAc                                                                                             <  7:349931/83‑1 (MQ=255)
                                                                                                                     gCGACGGGAAGTAGCGGGTCATCGGCCACAGGGCGCTGAAGGTCAGGCCGGCTTCTTCTTCAGCCTCGCGATGGGCGACTTCCTCGGGCTGCTCGTCTTTGTCGATCAGCCCGGCAACCATCTCGATCAGCCAGGGGTTGGCGACCTTg                       <  1:162747/149‑1 (MQ=255)
                                                                                                                       gACGGGAAGTAGCGGGTCATCGGCCACAGGGCGCTGAAGGTCAGGCCGGCTTCTTCTTCAGCCTCGCGATGGGCGACTTCCTCGGGCTGCTCGTCTTTGTCGATCAGCCCGGCAACCATCTCGATCAGCCAGGGGTTGGCGACCTTGTc                     <  6:94394/149‑1 (MQ=255)
                                                                                                                       gACGGGAAGTAGCGGGTCATCGGCCACAGGGCGCTGAAGGTCAGGCCGGCTTCTTCTTCAGCCTCGCGATGGGCGACTTCCTCGGGCTGCTCGTCTTTGTCGATCAGCCCGGCAACCATCTCGATCAGCCAGGCGTTGGCGACCTTGt                      >  7:72468/1‑148 (MQ=255)
                                                                                                                           ggAAGTAGCGGGTCATCGGCCACAGGGCGCTGAAGGTCAGGCCGGCTTCTTCTTCAGCCTCGCGATGGGCGACTTCCTCGGGCTGCTCGTCTTTGTCGATCAGCCCGGCAACCATCTCGATCAGCCAGGGGTTGGCGACCTTGTCCAgg                 >  2:16705/1‑149 (MQ=255)
                                                                                                                            gAAGTAGCGGGTCATCGGCCACAGGGCGCTGAAGGTCAGGCCGGCTTCTTCTTCAGCCTCGCGATGGGCGACTTCCTCGGGCTGCTCGTCTTTGTCGATCAGCCCGGCAACCATCTCGATCAGCCAGGGGTTGGCGACCTTGTCCAggg                <  5:353207/149‑1 (MQ=255)
                                                                                                                             aaGTAGCGGGTCATCGGCCACAGGGCGCTGAAGGTCAGGCCGGCTTCTTCTTCCGCCTCGCGATGGGCGACTTCCTCGGGCTGCTCGTCTTTGTCGATCAGCCCGGCAACCATCTCGATCAGCCAGGGGTTGGCGACCTTGTCCAGGgc               <  4:90739/149‑1 (MQ=255)
                                                                                                                               gTAGCGGGTCATCGGCCACAGGGCGCTGAAGGTCAGGCCGGCTTCTTCTTCAGCCTCGCGATGGGCGACTTCCTCGGGCTGCTCGTCTTTGTCGATCAGCCCGGCAACCATCTCGATCAGCCAGGGGTTGGCGAcc                          >  4:337288/1‑136 (MQ=255)
                                                                                                                               gTAGCGGGTCATCGGCCACAGGGCGCTGAAGGTCAGGCCGGCTTCTTCTTCAGCCTCGCGATGGGCGACTTCCTCGGGCTGCTCGTCTTTGTCGATCAGCCCGGCAACCATCTCGATCAGCCAGGGGTTGGCGAcc                          <  3:337288/136‑1 (MQ=255)
                                                                                                                                tAGCGGGTCATCGGCCACAGGGCGCTGAAGGTCAGGCCGGCTTCTTCTTCAGCCTCGCGATGGGCGACTTCCTCGGGCTGCTCGTCTTTGTCGATCAGCCCGGCAACCATCTCGATCAGCCAGGGGTTGGCGACCTTGTCCAGGGCGcc            >  4:307038/1‑149 (MQ=255)
                                                                                                                                tAGCGGGTCATCGGCCACAGGGCGCTGAAGGTCAGGCCGGCTTCTTCTTCAGCCTCGCGATGGGCGACTTCCTCGGGCTGCTCGTCTTTGTCGATCAGCCCGGCAACCATCTCGATCAGCCAGGGGGTGGCGACCTTGTCCAGGGCGcc            <  5:330659/149‑1 (MQ=255)
                                                                                                                                      gTCATCGGCCACAGGGCGCTGAAGGTCAGGCCGGCTTCTTCTTCAGCCTCGCGATGGGCGACTTCCTCGGGCTGCTCGTCTTTGTCGATCAGCCCGGCAACCATCTCGATCAGCCAGGGGTTGGCGACCTTGTCCAGGGCGCCCAcgcg      >  6:27888/1‑149 (MQ=255)
                                                                                                                                      gTCATCGGCCACAGGGCGCTGAAGGTCAGGCCGGCTTCTTCTTCAGCCTCGCGATGGGCGACTTCCTCGGGCTGCTCGTCTTTGTCGATCAGCCCGGCAACCATCTCGATCAGCCAGGGGTTGGCGACCTTGTCCAGGGCGCCCAcgcg      <  5:365174/149‑1 (MQ=255)
                                                                                                                                          tCGGCCACAGGGCGCTGAAGGTCAGGCCGGCTTCTTCTTCAGCCTCGCGATGGGCGACTTCCTCGGGCTGCTCGTCTTTGTCGATCAGCCCGGCAACCATCTCGATCAGCCAGGGGTTGGCGACCTTGTCCAGGGCGCCCACGCGGAAc  >  8:32451/1‑149 (MQ=255)
                                                                                                                                           |                                                                                                                                                   
ACGCCCACACGCGTACGCGAATGTCTTCGCCCTCTTCCTCCAGGCCATGCAGCCCTCCCGCGCCCTCGCTGCTGCAACGGCCGAGGAACAGATGAACGTATTCGTCACTGCCGCCAGGCGACGGGAAGTAGCGGGTCATCGGCCACAGGGCGCTGAAGGTCAGGCCGGCTTCTTCTTCAGCCTCGCGATGGGCGACTTCCTCGGGCTGCTCGTCTTTGTCGATCAGCCCGGCAACCATCTCGATCAGCCAGGGGTTGGCGACCTTGTCCAGGGCGCCCACGCGGAAC  >  NC_002947/5591365‑5591651

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑