Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,592,890 G→A 7.4% A388A (GCC→GCT)  PP_4921 ← NCS1 family transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,592,8900GA 7.4% 157.5 / 2.2 54A388A (GCC→GCT) PP_4921NCS1 family transporter
Reads supporting (aligned to +/- strand):  ref base G (21/29);  new base A (2/2);  total (23/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.87e-01

ATCCCCTACCCGCGCCTGAATGGCGTACTCAAGCCTGAACTGTTTCCCGGCCGCGGCTGAGGCTTAGCAGCCCATGCAGCACGCCTGCCAGCAGCAATGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAA  >  NC_002947/5592742‑5593012
                                                                                                                                                    |                                                                                                                          
aTCCCCTACCCGCGCCTGAATGGCGTACTCAAGCCTGAACTGTTTCCCGGCCGCGGCTGAGGCTTAGCAGCCCATGCAGCACGCCTGCCAGCAGCAATGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAg                                                                                                                            <  2:71078/149‑1 (MQ=255)
   cccTACCCGCGCCTGAATGGCGTACTCAAGCCTGAACTGTTTCCCGGCCGCGGCTGAGGCTTAGCAGCCCATGCAGCACGCCTGCCAGCAGCAATGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAgg                                                                                                                           <  4:115507/147‑1 (MQ=255)
   cccTACCCGCGCCTGAATGGCGTACTCAAGCCTGAACTGTTTCCCGGCCGCGGCTGAGGCTTAGCAGCCCATGCAGCACGCCTGCCAGCAGCAATGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAgg                                                                                                                           >  3:115507/1‑147 (MQ=255)
    ccTACCCGCGCCTGAATGGCGTACTCAAGCCTGAACTGTTTCCCGGCCGCGGCTGAGGCTTAGCAGCCCATGCAGCACGCCTGCCAGCAGCAATGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAg                                                                                                                            >  8:58894/1‑145 (MQ=255)
    ccTACCCGCGCCTGAATGGCGTACTCAAGCCTGAACTGTTTCCCGGCCGCGGCTGAGGCTTAGCAGCCCATGCAGCACGCCTGCCAGCAGCAATGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAg                                                                                                                            <  7:58894/145‑1 (MQ=255)
     cTACCCGCGCCTGAATGGCGTACTCAAGCCTGAACTGTTTCCCGGCCGCGGCTGAGGCTTAGCAGCCCATGCAGCACGCCTGCCAGCAGCAATGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGccac                                                                                                                       >  4:332973/1‑149 (MQ=255)
         ccGCGCCTGAATGGCGTACTCAAGCCTGAACTGTTTCCCGGCCGCGGCTGAGGCTTAGCAGCCCATGCAGCACGCCTGCCAGCAGCAATGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACt                                                                                                                   >  5:325687/1‑149 (MQ=255)
         ccGCGCCTGAATGGCGTACTCAAGCCTGAACTGTTTCCCGGCCGCGGCTGAGGCTTAGCAGCCCATGCAGCACGCCTGCCAGCAGCAATGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACt                                                                                                                   >  1:41210/1‑149 (MQ=255)
          cgcgCCTGAATGGCGTACTCAAGCCTGAACTGTTTCCCGGCCGCGGCTGAGGCTTAGCAGCCCATGCAGCACGCCTGCCAGCAGCAATGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTc                                                                                                                  <  6:325687/149‑1 (MQ=255)
            cgcCTGAATGGCGTACTCAAGCCTGAACTGTTTCCCGGCCGCGGCTGAGGCTTAGCAGCCCATGCAGCACGCCTGCCAGCAGCAATGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCg                                                                                                                <  8:230573/149‑1 (MQ=255)
             gcCTGAATGGCGTACTCAAGCCTGAACTGTTTCCCGGCCGCGGCTGAGGCTTAGCAGCCCATGCAGCACGCCTGCCAGCAGCAATGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCgg                                                                                                               <  3:99732/149‑1 (MQ=255)
               cTGAATGGCGTACTCAAGCCTGAACTGTTTCCCGGCCGCGGCTGAGGCTTAGCAGCCCATGCAGCACGCCTGCCAGCAGCAATGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTAGCGGCAATCAGGTGATAGGCCACCACTCCGGt                                                                                                              >  1:104358/1‑148 (MQ=255)
                  aaTGGCGTACTCAAGCCTGAACTGTTTCCCGGCCGCGGCTGAGGCTTAGCAGCCCATGCAGCACGCCTGCCAGCAGCAATGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAAc                                                                                                          <  3:332973/149‑1 (MQ=255)
                      gCGTACTCAAGCCTGAACTGTTTCCCGGCCGCGGCTGAGGCTTAGCAGCCCATGCAGCACGCCTGCCAGCAGCAATGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAgg                                                                                                      <  2:41210/149‑1 (MQ=255)
                       cGTACTCAAGCCTGAACTCTTTCCCGGCCGTGGCAGAGGCTTAGCAGCCCATGCAGCACGCCTGCCAGCAGCAATGCGGGCAGGGTGGCGCCCAGTTCTGGCGCCTTGGCGGCAATCAGGTCATAGGCCCCCACTCCGGTAAACCAGGc                                                                                                     >  4:279955/1‑149 (MQ=255)
                        gTACTCAAGCCTGAACTGTTTCCCGGCCGCGGCTGAGGCTTAGCAGCCCATGCAGCACGCCTGCCAGCAGCAATGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGcc                                                                                                    <  2:349129/149‑1 (MQ=255)
                         tACTCAAGCCTGAACTGTTTCCCGGCCGCGGCTGAGGCTTAGCAGCCCATGCAGCACGCCTGCCAGCAGCAATGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGcca                                                                                                   <  3:12486/149‑1 (MQ=255)
                            tCAAGCCTGAACTGTTTCCCGGCCGCGGCTGAGGCTTAGCAGCCCATGCAGCACGCCTGCCAGCAGCAATGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGccacc                                                                                                 >  2:340711/1‑148 (MQ=255)
                              aaGCCTGAACTGTTTCCCGGCCGCGGCTGAGGCTTAGCAGCCCATGCAGCACGCCTGCCAGCAGCAATGCGGGCAGGGTGGCGCCGAGTTCGGGCGGCTTGTCGGCAATCAGGTGTTGGGCGCCGACTCCGGTAACACAGGCCACCAAc                                                                                              >  5:235948/1‑149 (MQ=255)
                              aaGCCTGAACTGTTTCCCGGCCGCGGCTGAGGCTTAGCAGCCCATGCAGCACGCCTGCCAGCAGCAATGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAAc                                                                                              >  8:10791/1‑149 (MQ=255)
                              aaGCCTGAACTGTTTCCCGGCCGCGGCTGAGGCTTAGCAGCCCATGCAGCACGCCTGCCAGCAGCAATGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAAc                                                                                              >  3:72546/1‑149 (MQ=255)
                                   tGAACTGTTTCCCGGCCGCGGCTGAGGCTTAGCAGCCCATGCAGCACGCCTGCCAGCAGCAATGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTg                                                                                         >  3:138138/1‑149 (MQ=255)
                                     aaCTGTGTCCCGGCCGCGGCTGAGGCTTAGCAGCCCATGCAGCACGCCTGCCAGCAGCAATGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGc                                                                                        <  8:301904/148‑1 (MQ=255)
                                                ggCCGCGGCTGAGGCTTAGCAGCCCATGCAGCACGCCTGCCAGCAGCAATGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCaa                                                                                <  7:19451/145‑1 (MQ=255)
                                                ggCCGCGGCTGAGGCTTAGCAGCCCATGCAGCACGCCTGCCAGCAGCAATGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCaa                                                                                >  8:19451/1‑145 (MQ=255)
                                                   cgcgGCTGAGGCTTAGCAGCCCATGCAGCACGCCTGCCAGCAGCAATGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTg                                                                         <  7:170404/149‑1 (MQ=255)
                                                      ggCTGAGGCTTAGCAGCCCATGCAGCACGCCTGCCAGCAGCAATGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGAcc                                                                      >  6:81468/1‑149 (MQ=255)
                                                      ggCTGAGGCTTAGCAGCCCATGCAGCACGCCTGCCAGCAGCAATGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGAcc                                                                      <  4:99455/149‑1 (MQ=255)
                                                       gCTGAGGCTTAGCAGCCCATGCAGCACGCCTGCCAGCAGCAATGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCt                                                                     <  2:187514/149‑1 (MQ=255)
                                                          gAGGCTTAGCAGCCCATGCAGCACGCCTGCCAGCAGCAATGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGAc                                                                       <  3:94108/144‑1 (MQ=255)
                                                          gAGGCTTAGCAGCCCATGCAGCACGCCTGCCAGCAGCAATGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGAc                                                                       >  4:94108/1‑144 (MQ=255)
                                                           aGGCTTAGCAGCCCATGCAGCACGCCTGCCAGCAGCAATGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAAGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGAc                                                                       >  7:241766/1‑143 (MQ=255)
                                                           aGGCTTAGCAGCCCATGCAGCACGCCTGCCAGCAGCAATGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAAGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGAc                                                                       <  8:241766/143‑1 (MQ=255)
                                                           aGGCTTAGCAGCCCATGCAGCACGCCTGCCAGCAGCAATGCGGGCAGGGTGGCGCCCAGTTCGAGCGCCTTGGCGGCAATCAGCTGATAAGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGAc                                                                       >  7:241768/1‑143 (MQ=255)
                                                           aGGATTAGCAGCCCATGCAGCACGCCTGCCAGCAGCAATGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAAGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGAc                                                                       <  8:241768/143‑1 (MQ=255)
                                                                        cATGCAGCACGCCTGCCAGCAGCAATGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGccac                                                                                                  >  5:315017/1‑103 (MQ=255)
                                                                        cATGCAGCACGCCTGCCAGCAGCAATGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGccac                                                                                                  <  6:315017/103‑1 (MQ=255)
                                                                         aTGCAGCACGCCTGCCAGCAGCAATGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTc                                                   <  7:10791/149‑1 (MQ=255)
                                                                               caCGCCTGCCAGCAGCAATGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGa                                             >  1:266802/1‑149 (MQ=255)
                                                                                  gCCTGCCAGCAGCAATGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGt                                                                                    <  6:26081/107‑1 (MQ=255)
                                                                                  gCCTGCCAGCAGCAATGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGACTGCCAGt                                                                                    >  5:26081/1‑107 (MQ=255)
                                                                                   ccTGCCAGCAGCAATGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCAc                                         <  4:138138/149‑1 (MQ=255)
                                                                                       ccAGCAGCAATGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAg                                     <  3:63415/149‑1 (MQ=255)
                                                                                         agcagcAATGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGa                                                 >  3:123297/1‑135 (MQ=255)
                                                                                         agcagcAATGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGa                                                 <  4:123297/135‑1 (MQ=255)
                                                                                          gcagcaATGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTgg                                  >  7:3379/1‑149 (MQ=255)
                                                                                          gcagcaATGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTgg                                  <  4:72546/149‑1 (MQ=255)
                                                                                           cagcaATGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGt                                 >  4:78211/1‑149 (MQ=255)
                                                                                                 tGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCa                           <  3:78211/149‑1 (MQ=255)
                                                                                                     ggCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGcac                       <  1:61692/149‑1 (MQ=255)
                                                                                                          ggTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGc                  <  2:266802/149‑1 (MQ=255)
                                                                                                              gcgcCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGaa              >  1:245928/1‑149 (MQ=255)
                                                                                                                gcCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGa                                             <  4:149475/116‑1 (MQ=255)
                                                                                                                gcCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGa                                             >  3:149475/1‑116 (MQ=255)
                                                                                                                     gTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGt       <  6:136163/149‑1 (MQ=255)
                                                                                                                      ttCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTg      <  1:340711/149‑1 (MQ=255)
                                                                                                                        cGGGCGCCTTGGCGGCATTCAGTTGATAGGCCACCACTCCGGTAATCCAGGCCACCAACGCCTGCCAGTGGAAGCCATTGACCTGTGCTGGCTGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGc     <  6:102387/148‑1 (MQ=255)
                                                                                                                         gggCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCg    <  2:245928/148‑1 (MQ=255)
                                                                                                                          ggCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGaa  <  8:3379/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                          
ATCCCCTACCCGCGCCTGAATGGCGTACTCAAGCCTGAACTGTTTCCCGGCCGCGGCTGAGGCTTAGCAGCCCATGCAGCACGCCTGCCAGCAGCAATGCGGGCAGGGTGGCGCCCAGTTCGGGCGCCTTGGCGGCAATCAGGTGATAGGCCACCACTCCGGTAAACCAGGCCACCAACGCCTGCCAGTGCAAGCCATTGACCTGTGCTGGCAGACGGCGTCGACGGACCACGTAGTGGTCCATCAGCACCACGCCGAACAGCGGTGCGAA  >  NC_002947/5592742‑5593012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑