Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,611,236 G→A 6.9% R77H (CGC→CAC)  PP_4931 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,611,2360GA 6.9% 175.1 / 2.1 58R77H (CGC→CAC) PP_4931hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (25/29);  new base A (2/2);  total (27/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

GGCTACTCGACAGTGCTGGTGGAGCGCGCCAGCCTTGGCGGCGAGCAGACCATCAAGTCGCAGGGCATCATCCACGGCGGCACCAAATACGCCCTGCACGGCGCCCTGACCGGCGCCTCGGAAGCC‑ATCGCCGACATGCCGCGCCGCTGGCGCGAGGCCTTGGCCGGCAACGGCGAACTCGACCTCAGTCGTACCCGCCTGCTCTCCGATGCCCACTACCTGTGGTCGCCGGGCACCTTGGCCGGCAACCTCACAAGCTTCTTCGCCAGCAAGGCCGTGCGCGGCCGGGTCGAC  >  NC_002947/5611091‑5611384
                                                                                                                                                  |                                                                                                                                                    
ggCTACTCGACAGTGCTGGTGGAGCGCGCCAGCCTTGGCGGCGAGCAGACCATCAAGTCGCAGGGCATCATCCACGGCGGCACCAAATACGCCCTGCACGGCGCCCTGACCGGCGCCTCGGAAGCC‑ATCGCCGACATGCCGCGCCGCTg                                                                                                                                                   <  2:218082/149‑1 (MQ=255)
 gCTACTCGACAGTGCTGGTGGAGCGCGCCAGCCTTGGCGGCGAGCAGACCATCAAGTCGCAGGGCATCATCCACGGCGGCACCAAATACGCCCTGCACGGCGCCCTGACCGGCGCCTCGGAAGCC‑ATCGCCGACATGCCGCGCCGCTgg                                                                                                                                                  <  2:269889/149‑1 (MQ=255)
   tACTCGACAGTGCTGGTGGAGCGCGCCAGCCTTGGCGGCGAGCAGACCATCAAGTCGCAGGGCATCATCCACGGCGGCACCAAATACGCCCTGCACGGCGCCCTGACCGGCGCCTCGGAAGCC‑ATCGCCGACATGCCGCGCCGCTGgcg                                                                                                                                                >  8:69781/1‑149 (MQ=255)
   tACTCGACAGTGCTGGTGGAGCGCGCCAGCCTTGGCGGCGAGCAGACCATCAAGTCGCAGGGCATCATCCACGGCGGCACCAAATACGCCCTGCACGGCGCCCTGACCGGCGCCTCGGAAGCC‑ATCGCCGACATGCCGCGCCGCTGgcg                                                                                                                                                >  2:218142/1‑149 (MQ=255)
   tACTCGACAGTGCTGGTGGAGCGCGCCAGCCTTGGCGGCGAGCAGACCATCAAGTCGCAGGGCATCATCCACGGCGGCACCAAATACGCCCTGCACGGCGCCCTGACCGGCGCCTCGGAAGCC‑ATCGCCGACATGCCGCGCCACTGgcg                                                                                                                                                >  5:2727/1‑149 (MQ=255)
      tCGACAGTGCTGGTGGAGCGCGCCAGCCTTGGCGGCGAGCAGACCATCAAGTCGCAGGGCATCATCCACGGCGGCACCAAATACGCCCTGCACGGCGCCCTGACCGGCGCCTCGGAAGCC‑ATCGCCGACATGCCGCGCCGCTGgcgc                                                                                                                                               >  6:276358/1‑147 (MQ=255)
        gACAGTGCTGGTGGAGCGCGCCAGCCTTGGCGGCGAGCAGACCATCAAGTCGCAGGGCATCATCCACGGCGGCACCAAATACGCCCTGCACGGCGCCCTGACCGGCGCCTCGGAAGCCAATCGCCGACATGCCGCGCCGCTGGCGCGAg                                                                                                                                            <  1:220696/149‑1 (MQ=255)
        gACAGTGCTGGTGGAGCGCGCCAGCCTTGGCGGCGAGCAGACCATCAAGTCGCAGGGCATCATCCACGGCGGCACCAAATACGCCCTGCACGGCGCCCTGACCGGCGCCTCGGAAGCC‑ATCGCCGACATGCCGCGCCGCTGGCGCGa                                                                                                                                             <  7:135684/147‑1 (MQ=255)
         aCAGTGCTGGTGGAGCGCGCCAGCCTTGGCGGCGAGCAGACCATCAAGTCGCAGGGCATCATCCACGGCGGCACCAAATACGCCCTGCACGGCGCCCTGACCGGCGCCTCGGAAGCC‑ATCGCCGACATGCCGCGCCGCTGGCGCGAGGc                                                                                                                                          >  8:83237/1‑149 (MQ=255)
             tGCTGGTGGAGCGCGCCAGCCTTGGCGGCGAGCAGACCATCAAGTCGCAGGGCATCATCCACGGCGGCACCAAATACGCCCTGCACGGCGCCCTGACCGGCGCCTCGGAAGCC‑ATCGCCGACATGCCGCGCCGCTGGCGCGAGGCCTTg                                                                                                                                      >  8:77611/1‑149 (MQ=255)
              gCTGGTGGAGCGCGCCAGCCTTGGCGGCGAGCAGACCATCAAGTCGCAGGGCATCATCCACGGCGGCACCAAATACGCCCTGCACGGCGCCCTGACCGGCGCCTCGGAAGCC‑ATCGCCGACATGCCGCGCCGCTGGCGCGAGGCCTTgg                                                                                                                                     >  4:145157/1‑149 (MQ=255)
                 ggtggAGCGCGCCAGCCTTGGCGGCGAGCAGACCATCAAGTCGCAGGGCATCATCCACGGCGGCACCAAATACGCCCTGCACGGCGCCCTGACCGGCGCCTCGGAAGCC‑ATCGCCGACATGCCGCGCCGCTGGCGCGAGGCCTTGGCCg                                                                                                                                  <  4:35774/149‑1 (MQ=255)
                  gtggAGCGCGCCAGCCTTGGCGGCGAGCAGACCATCTAGTCGCAGGGCATCATCCACGGCGGCACCAAATACGCCCTGCACGGCGCCCTGACCGGCGCCTCGGAAGCC‑ATCGCCGACATGCCGCGCCGCTGGCGCGAGGCCTTGGCCg                                                                                                                                  <  2:115663/148‑1 (MQ=255)
                            ccAGCCTTGGCGGCGAGCAGACCATCAAGTCGCAGGGCATCATCCACGGCGGCACCAAATACGCCCTGCACGGCGCCCTGACCGGCGCCTCGGAAGCC‑ATCGCCGACATGCCGCGCCGCTGGCGCGAGGCCTTGGCCGGCAACGGCGaa                                                                                                                       <  6:130056/149‑1 (MQ=255)
                                  ttGGCGGCGAGCAGACCATCAAGTCGCAGGGCATCATCCACGGCGGCACCAAATACGCCCTGCACGGCGCCCTGACCGGCGCCTCGGAAGCC‑ATCGCCGACATGCCGCGCCGCTGGCGCGAGGc                                                                                                                                          >  4:230856/1‑124 (MQ=255)
                                  ttGGCGGCGAGCAGACCATCAAGTCGCAGGGCATCATCCACGGCGGCACCAAATACGCCCTGCACGGCGCCCTGACCGGCGCCTCGGAAGCC‑ATCGCCGACATGCCGCGCCGCTGGCGCGAGGc                                                                                                                                          <  3:230856/124‑1 (MQ=255)
                                            gCAGACCATCAAGTCGCAGGGCATCATCCACGGCGGCACCAAATACGCCCTGCACGGCGCCCTGACCGGCGCCTCGGAAGCC‑ATCGCCGACATGCCGCGCCGCTGGCGCGAGGCCTTGGCCGGCAACGGCGAACTCGACCTCAGTCGTa                                                                                                       <  1:218142/149‑1 (MQ=255)
                                                  cATCAAGTCGCAGGGCATCATCCACGGCGGCACCAAATACGCCCTGCACGGCGCCCTGACCGGCGCCTCGGAAGCC‑ATCGCCGACATGCCGCGCCGCTGGCGCGAGGCCTTGGCCGGCAACGGCGAACTCGACCTCAGTc                                                                                                          >  7:231744/1‑140 (MQ=255)
                                                  cATCAAGTCGCAGGGCATCATCCACGGCGGCACCAAATACGCCCTGCACGGCGCCCTGACCGGCGCCTCGGAAGCC‑ATCGCCGACATGCCGCGCCGCTGGCGCGAGGCCTTGGCCGGCAACGGCGAACTCGACCTCAGTc                                                                                                          <  8:231744/140‑1 (MQ=255)
                                                  cATCAAGTCGCAGGGCATCATCCACGGCGGCACCAAATACGCCCTGCACGGCGCCCTGACCGGCGCCTCGGAAGCC‑ATCGCCGACATGCCGCGCCGCTGGCGCGAGGCCTTGGCCGGCAACGGCGAACTCGACCTCAGTCGTACCCGcc                                                                                                 >  6:219498/1‑149 (MQ=255)
                                                   aTCAAGTCGCAGGGCATCATCCACGGCGGCACCAAATACGCCCTGCACGGCGCCCTGACCGGCGCCTCGGAAGCC‑ATCGCCGACATGCCGCGCCGCTGGCGCGAGGCCTTGGCCGGCAACGGCGAACTCGACCTCAGTCGTACCCGCCt                                                                                                <  7:69781/149‑1 (MQ=255)
                                                    tCAAGTCGCAGGGCATCATCCACGGCGGCACCAAATACGCCCTGCACGGCGCCCTGACCGGCGCCTCGGAAGCC‑ATCGCCGACATGCCGCGCCGCTGGCGCGAGGCCTTGGCCGGCAACGGCGAACTCGACCTCAGTCGTACCCGCCTg                                                                                               >  5:148012/1‑149 (MQ=255)
                                                    tCAAGTCGCAGGGCATCATCCACGGCGGCACCAAATACGCCCTGCACGGCGCCCTGACCGGCGCCTCGGAAGCC‑ATCGCCGACATGCCGCGCCGCTGGCGCGAGGCCTTGGCCGGCAACGGCGAACTCGACCTCAGTCGTACCCGCCTg                                                                                               <  5:184522/149‑1 (MQ=255)
                                                    tCAAGTCGCAGGGCATCATCCACGGCGGCACCAAATACGCCCTGCACGGCGCCCTGACCGGCGCCTCGGAAGCC‑ATCGCCGACATGCCGCGCCACTGGCGCGAGGCCTTGGCCGGCAACGGCGAACTCGACCTCAGTCGTACCCGCCTg                                                                                               >  4:201575/1‑149 (MQ=255)
                                                        gTCGCAGGGCATCATCCACGGCGGCACCAAATACGCCCTGCACGGCGCCCTGACCGGCGCCTCGGAAGCC‑ATCGCCGACAGGCCGCGCCGCTGGCGCGAGGCCTTGGCCGGCAACGGCGCACTCGACCTCAGACGTGCCCGCCTGctct                                                                                           >  4:189218/1‑149 (MQ=255)
                                                            cAGGGCATCATCCACGGCGGCACCAAATACGCCCTGCACGGCGCCCTGACCGGCGCCTCGGAAGCC‑ATCGCCGACATGCCGCGCCGCTGGCGCGAGGCCTTGGCCGGCAACGGCGAACTCGACCTCAGTCGTACCCGCCTGCTCTCCGa                                                                                       <  7:83237/149‑1 (MQ=255)
                                                             aGGGCATCATCCACGGCGGCACCAAATACGCCCTGCACGGCGCCCTGACCGGCGCCTCGGAAGCC‑ATCGCCGACATGCCGCGCCGCTGGCGCGAGGCCTTGGCCGGCAACGGCGAACTc                                                                                                                    <  6:8526/119‑1 (MQ=255)
                                                             aGGGCATCATCCACGGCGGCACCAAATACGCCCTGCACGGCGCCCTGACCGGCGCCTCGGAAGCC‑ATCGCCGACATGCCGCGCCGCTGGCGCGAGGCCTTGGCCGGCAACGGCGAACTc                                                                                                                    >  5:8526/1‑119 (MQ=255)
                                                                 catcatCCACGGCGGCACCAAATACGCCCTGCACGGCGCCCTGACCGGCGCCTCGGAAGCC‑ATCGCCGACATGCCGCGCCGCTGGCGCGAGGCCTTGGCCGGCAACGGCGAACTCGACCTCAGTCGTACCCGCCTGCTCTCCGATGccc                                                                                  <  7:77611/149‑1 (MQ=255)
                                                                         aCGGCGGCACCAAATACGCCCTGCACGGCGCCCTGACCGGCGCCTCGGAAGCC‑ATCGCCGACATGCCGCGCCGCTGGCGCGAGGCCTTGGCCGGCAACGGCGAACTCGACCTCAGTCGTACCCGCCTGCTCTCCGATGCCCACTACCtg                                                                          >  6:361332/1‑149 (MQ=255)
                                                                         aCGGCGGCACCAAATACGCCCTGCACGGCGCCCTGACCGGCGCCTCGGAAGCC‑ATCGCCGACATGCCGCGCCGCTGGCGCGAGGCCTTGGCCGGCAACGGCGAACTCGACCTCAGTCGTACCCGCCTGCTCTCCGATGCCCACTACCtg                                                                          >  1:67261/1‑149 (MQ=255)
                                                                         aCGGCGGCACCAAATACGCCCTGCACGGCGCCCTGACCGGCGCCTCGGAAGCC‑ATCGCCGACATGCCGCGCCGCTGGCGCGAGGCCTTGGCCGGCAACGGCGAACTCGACCTCAGTCGTACCCGCCTGCTCTCCGATGCCCACTACCtg                                                                          <  2:198199/149‑1 (MQ=255)
                                                                         aCGGCGGCACCAAATACGCCCTGCACGGCGCCCTGACCGGCGCCTCGGAAGCC‑ATCGCCGACATGCCGCGCCGCTGGCGCGAGGCCTTGGCCGGCAACGGCGAACTCGACCTCAGTCGTACCCGCCTGCTCTCCGATGCCCACTACCtg                                                                          >  3:190844/1‑149 (MQ=255)
                                                                           ggcggcACCAAATACGCCCTGCACGGCGCCCTGACCGGCGCCTCGGAAGCC‑ATCGCCGACATGCCGCGCCGCTGGCGCGAGGCCTTGGCCGGCAACGGCGAACTCGACCTCAGTCGTACCCGCCTGCTCTCCGATGCCCACTACCtgtg                                                                        <  5:276358/149‑1 (MQ=255)
                                                                           ggcggcACCAAATACGCCCTGCACGGCGCCCTGACCGGCGCCTCGGAAGCC‑ATCGCCGACATGCCGCGCCGCTGGCGCGAGGCCTTGGCCGGCAACGGCGAACTCGACCTCAGTCGTACCCGCCTGCTCTCCGATGCCCACTACCtgtg                                                                        <  6:326935/149‑1 (MQ=255)
                                                                           ggcggcACCAAATACGCCCTGCACGGCGCCCTGAC‑‑ACGCCTCGGAAGCC‑ATCGCCGACATGCCGCGCCGCTGGCGCGAGGCCTTGGCCGGCAACGGCGAACTCGACCTCAGTCGTACCCGCCTGCTCTCCGATGCCCACTACCTGTGGt                                                                      <  8:123633/149‑1 (MQ=255)
                                                                            gcggcACCAAATACGCCCTGCACGGCGCCCTGACCGGCGCCTCGGAAGCC‑ATCGCCGACATGCCGCGCCGCTGGCGCGAGGCCTTGGCCGGCAACGGCGAACTCGACCTCAGTCGTACCCGCCTGCTCTCCGATGCCCACTACCTGTgg                                                                       <  4:190844/149‑1 (MQ=255)
                                                                              ggcACCAAATACGCCCTGCACGGCGCCCTGACCGGCGCCTCGGAAGCC‑ATCGCCGACATGCCGCGCCGCTGGCGCGAGGCCTTGGCCGGCAACGGCGAACTCGACCTCAGTCGTACCCGCCTGCTCTCCGATGCCCACTACCTGTGGTc                                                                     >  4:42432/1‑149 (MQ=255)
                                                                               gcACCAAATACGCCCTGCACGGCGCCCTGACCGGCGCCTCGGAAGCC‑ATCGCCGACATGCCGCGCCGCTGGCGCGAGGCCTTGGCCGGCAACGGCGAACTCGACCTCAGTCGTACCCGCCTGCTCTCCGATGCCCACTACCTGTGGTCg                                                                    >  8:130725/1‑149 (MQ=255)
                                                                                 aCCAAATACGCCCTGCACGGCGCCCTGACCGGCGCCTCGGAAGCC‑ATCGCCGACATGCCGCGCCGCTGGCGCGAGGCCTTGGCCGGCAACGGCGAACTCGACCTCAGTCGTACCCGCCTGCTCTCCGATGCCCACTACCTGTGGTCGcc                                                                  >  6:198719/1‑149 (MQ=255)
                                                                                 aCCAAATACGCCCTGCACGGCGCCCTGACCGGCGCCTCGGAAGCC‑ATCGCCGACATGCCGCGCCGCTGGCGCGAGGCCTTGGCCGGCAACGGCGAACTCGACCTCAGTCGTACCCGCCTGCTCCCCGATGCCCACTACCTGTGGTCGcc                                                                  >  6:271611/1‑149 (MQ=255)
                                                                                   cAAATACGCCCTGCACGGCGCCCTGACCGGCGCCTCGGAAGCC‑ATCGCCGACATGCCGCGCCGCTGGCGCGAGGCCTTGGCCGGCAACGGCGAACTCGACCTCAGTCGTACCCGCCTGCTCTCCGATGCCCACTACCTGTGGTCGCCgg                                                                <  3:145157/149‑1 (MQ=255)
                                                                                         cGCCCTGCACGGCGCCCTGACCGGCGCCTCGGAAGCC‑ATCGCCGACATGCCGCGCCGCTGGCGCGAGGCCTTGGCCGGCAACGGCGAACTCGACCTCAGTCGTACCCGCCTGCTCTCCGATGCCCACTACCTGTGGTCGCCGGGCACCt                                                          <  5:328268/149‑1 (MQ=255)
                                                                                                cACGGCGCCCTGACCGGCGCCTCGGAAGCC‑ATCGCCGACATGCCGCGCCGCTGGCTCGAGGCCTTGGCCGGCAACGGCGAACTCGACCTCAGTCGTACCCGCCTGCTCTCCGATGCCCACTACCTGTGGTCGCCGGGCACCTTGGCCg                                                    <  7:130725/148‑1 (MQ=255)
                                                                                                cACGGCGCCCTGACCGGCGCCTCGGAAGCC‑ATCGCCGACATGCCGCGCCGCTGGCGCGAGGCCTTGGCCGGCAACGGCGAACTCGACCTCAGTCGAACCCGCCTGCTCTCCGATGCCCACTACCTGTGGTCGCCGGGCACCTTGGCCg                                                    <  3:42432/148‑1 (MQ=255)
                                                                                                cACGGCGCCCTGACCGGCGCCTCGGAAGCC‑ATCGCCGACATGCCGCGCCACTGGCGCGAGGCCTTGGCCGGCAACGGCGAACTCGACCTCAGTCGTACCCGCCTGCTCTCCGATGCCCACTACCTGTGGTCGCCGGGCACCTTGGcc                                                     <  3:201575/147‑1 (MQ=255)
                                                                                                   ggCGCCCTGACCGGCGCCTCGGAAGCC‑ATCGCCGACATGCCGCGCCACTGGCGCGAGGCCTTGGCCGGCAACGGCGAACTCGACCTCAGTCGTACCCGCCTGCTCTCCGATGCCCACTACCTGTGGTCGCCGGGCACCTTGGCCGGCaa                                                <  6:2727/149‑1 (MQ=255)
                                                                                                      gcCCTGACCGGCGCCTCGGAAGCC‑ATCGCCGACATGCCGCGCCGCTGGCGCGAGGCCTTGGCCGGCAACGGCGAACTCGACCTCAGTCGTACCCGCCTGCTCTCCGATGCCCACTACCTGTGGTTGCCGGCCAAGGTGCCCGGCGAcca                                             <  5:219498/149‑2 (MQ=255)
                                                                                                         cTGACCGGCGCCTCGGAAGCC‑ATCGCCGACATGCCGCGCCGCTGGCGCGAGGCCTTGGCCGGCAACGGCGAACTCGACCTCAGTCGTACCCGCCTGCTCTCCGATGCCCACTACCTGTGGTCGCCGGGCACCTTGGCCGGCAACCTca                                           >  6:58042/1‑148 (MQ=255)
                                                                                                                gcgcCTCGGAAGCC‑ATCGCCGACATGCCGCGCCGCTGGCGCGAGGCCTTGGCCGGCAACGGCGAACTCGACCTCAGTCGTACCCGCCTGCTCTCCGATGCCCACTACCTGTGGTCGCCGGGCACCTTGGc                                                      >  6:53474/1‑130 (MQ=255)
                                                                                                                gcgcCTCGGAAGCC‑ATCGCCGACATGCCGCGCCGCTGGCGCGAGGCCTTGGCCGGCAACGGCGAACTCGACCTCAGTCGTACCCGCCTGCTCTCCGATGCCCACTACCTGTGGTCGCCGGGCACCTTGGc                                                      <  5:53474/130‑1 (MQ=255)
                                                                                                                gcgcCTCGGAAGCC‑ATCGCCGACATGCCGCGCCGCTGGCGCGAGGCCTTGGCCGGCAACGGCGAACTCGACCTCAGTCGTACCCGCCTGCTCTCCGATGCCCACTACCTGTGGTCGCCGGGCACCTTGGCCGGCAACCTCACAAGcttc                                   <  7:121445/149‑1 (MQ=255)
                                                                                                                gcgcCTCGGAAGCC‑ATCGCCGACATGCCGCGCCGCTGGCGCGAGGCCTTGGCCGGCAACGGCGAACTCGACCTCAGTCGTACCCGCCTGCTCTCCGATGCCCACTACCTGTGGTCGCCGGGCACCTTGGCCGGCAACCTCACAAGcttc                                   >  4:8329/1‑149 (MQ=255)
                                                                                                                   ccTCGGAAGCC‑ATCGCCGACATGCCGCGCCGCTGGCGCGAGGCCTTGGCCGGCAACGGCGAACTCGACCTCAGTCGTACCCGCCTGCTCTCCGATGCCCACTACCTGTGGTTGCCGGGCACCTTGGCCGGCAACCTCACAAGCttcttc                                >  1:324252/1‑149 (MQ=255)
                                                                                                                       ggAAGCC‑ATCGCCGACATGCCGCGCCGCTGGCGCGAGGCCTTGGCCGGCAACGGCGAACTCGACCTCAGTCGTACCCGCCTGCTCTCCGATGCCCACTACCTGTGGTCGCCGGGCACCTTGGCCGGCAACCTCACAAGCTTCTTCGc                              <  7:130891/147‑1 (MQ=255)
                                                                                                                       ggAAGCC‑ATCGCCGACATGCCGCGCCGCTGGCGCGAGGCCTTGGCCGGCAACGGCGAACTCGACCTCAGTCGTACCCGCCTGCTCCCCGATGCCCACTACCTGTGGTCGCCGGGCACCTTGGCCGGCAACCTCACAAGCTTCTTCGCCa                            <  5:271611/149‑1 (MQ=255)
                                                                                                                        gAAGCC‑ATCGCCGACATGCCGCGCCGCTGGCGCGAGGCCTTGGCCGGCAACGGCGAACTCGACCTCAGTCGTACACGCCTGCACTCCCATGCCCACTACCTGTGGTCGCCGGGCACCTTGGCCGGCAccc                                              >  2:93082/1‑130 (MQ=255)
                                                                                                                                     gACATGCCGCGCCGCTGGCGCGAGGCCTTGGCCGGCAACGGCGAACTCGACCTCAGTCGTACCCGCCTGCTCTCCGATGCCCACTACCTGTGGTCGCCGGGCACCTTGGCCGGCAACCTCACAAGCTTCTTCGCCAGCAAGGCCGTgcg               >  4:18257/1‑149 (MQ=255)
                                                                                                                                             gcgccgcTGGCGCGAGGCCTTGGCCGGCAACGGCGAACTCGACCTCAGTCGTACCCGCCTGCTCTCCGATGCCCACTACCTGTGGTCGCCGGGCACCTTGGCCGGCAACCTCACAAGCTTCTTCGCCAGCAAGGCCGTGCGCGGCCggg       <  6:225064/149‑1 (MQ=255)
                                                                                                                                               gccgcTGGCGCGAGGCCTTGGCCGGCAACGGCGAACTCGACCTCAGTCGTACCCGCCTGCTCTCCGATGCCCACTACCTGTGGTCGCCGGGCACCTTGGCCGGCAACCTCACAAGCTTCTTCGCCAGCAAGGCCGTGCGCGGCCGGGTc     <  5:361332/149‑1 (MQ=255)
                                                                                                                                                 cgcTGGCGCGAGGCCTTGGCCGGCAACGGCGAACTCGACCTCAGTCGTACCCGCCTGCTCTCCGATGCCCACTACCTGTGGTCGCCGGGCACCTTGGCCGGCAACCTCACAAg                                       <  1:140217/113‑1 (MQ=255)
                                                                                                                                                 cgcTGGCGCGAGGCCTTGGCCGGCAACGGCGAACTCGACCTCAGTCGTACCCGCCTGCTCTCCGATGCCCACTACCTGTGGTCGCCGGGCACCTTGGCCGGCAACCTCACAAg                                       >  2:140217/1‑113 (MQ=255)
                                                                                                                                                  gcTGGCGCGAGGCCTTGGCCGGCAACGGCGAACTCGACCTCAGTCGTACCCGCCTGCTCTCCGATGCCCACTACCTGTGGTCGCCGGGCACCTTGGCCGGCAACCTCACAAGCTTCTTCGCCAGCAAGGCCGTGCGCGGCCGGGTCGAc  <  3:8329/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
GGCTACTCGACAGTGCTGGTGGAGCGCGCCAGCCTTGGCGGCGAGCAGACCATCAAGTCGCAGGGCATCATCCACGGCGGCACCAAATACGCCCTGCACGGCGCCCTGACCGGCGCCTCGGAAGCC‑ATCGCCGACATGCCGCGCCGCTGGCGCGAGGCCTTGGCCGGCAACGGCGAACTCGACCTCAGTCGTACCCGCCTGCTCTCCGATGCCCACTACCTGTGGTCGCCGGGCACCTTGGCCGGCAACCTCACAAGCTTCTTCGCCAGCAAGGCCGTGCGCGGCCGGGTCGAC  >  NC_002947/5611091‑5611384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑