Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,631,116 A→T 10.1% K216* (AAG→TAG)  putA → proline dehydrogenase/1‑pyrroline‑5‑carboxylate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,631,1160AT10.1% 105.3 / 2.5 39K216* (AAG→TAG) putAproline dehydrogenase/1‑pyrroline‑5‑carboxylate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (17/18);  new base T (2/2);  total (19/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGCTGCGTATCCCCGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATCCGCAAGGGCGTCGACATGGCCATGCGCCTGATGGGTGAGCAGTTCGTCACCGGCGAGACCATTGCCG  >  NC_002947/5630978‑5631263
                                                                                                                                          |                                                                                                                                                   
ctgctgCGTATCCCCGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAg                                                                                                                                           <  3:67160/149‑1 (MQ=255)
   ctgcGTATCCCCGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCAGCTGGGGGCTGCTGCTGACCAGCTTGCCGGTCAGCa                                                                                                                                         <  6:369092/148‑1 (MQ=255)
    tgcGTATCCCCGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCAcc                                                                                                                                       >  4:276115/1‑149 (MQ=255)
         aTCCCCGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATaa                                                                                                                                  >  3:116429/1‑149 (MQ=255)
          tCCCCGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATaa                                                                                                                                  >  5:198287/1‑148 (MQ=255)
          tCCCCGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATaa                                                                                                                                  <  6:198287/148‑1 (MQ=255)
          tCCCCGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAAc                                                                                                                                 >  3:29047/1‑149 (MQ=255)
            cccGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCAGGGGGCTGCTGCTGACCGGCTAGCGGGTCAGCAccccgaagg                                                                                                                                >  8:328829/1‑142 (MQ=255)
             ccGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGa                                                                                                                               >  3:90411/1‑148 (MQ=255)
                 cAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCg                                                                                                                          <  3:306535/149‑1 (MQ=255)
                      gTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTc                                                                                                                     <  4:90411/149‑1 (MQ=255)
                         cccGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCAc                                                                                                                   <  2:360826/148‑1 (MQ=255)
                           cGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCAcctc                                                                                                                <  6:331566/149‑1 (MQ=255)
                             tGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAAGGAAACCGGCCTCAcctcct                                                                                                              <  5:300849/149‑1 (MQ=255)
                                tGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGc                                                                                                           >  1:131073/1‑149 (MQ=255)
                                         ccGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCg                                                                                                            <  3:221763/139‑1 (MQ=255)
                                         ccGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGACCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCACCACCCATAACGAAACCGGCCACACCTCCACg                                                                                                            >  4:221763/1‑139 (MQ=255)
                                                 aGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGc                                                                                          <  7:328829/149‑1 (MQ=255)
                                                       gTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAgc                                                                                    >  3:224806/1‑149 (MQ=255)
                                                           cGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAgcggcg                                                                                <  4:116429/149‑1 (MQ=255)
                                                           cGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAgcggc                                                                                 >  2:271427/1‑148 (MQ=255)
                                                             gCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTCCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAg                                                                              >  7:50481/1‑149 (MQ=255)
                                                                          gCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATCCGc                                                                  >  5:309149/1‑148 (MQ=255)
                                                                                  gCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATCCGCAAGGGCGTc                                                         <  3:276115/149‑1 (MQ=255)
                                                                                      cAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATCCGCAAGGGCGTCGACa                                                     >  2:25340/1‑149 (MQ=255)
                                                                                                cTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATCCGCAAGGGCGTCGACATGGCCATgcg                                           <  6:309149/149‑1 (MQ=255)
                                                                                                      gTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATCCGCAAGGGCGTCGACATGGCCATGCGCCTGa                                      <  4:29047/148‑1 (MQ=255)
                                                                                                          aCGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATCCGCAAGGGCGTCGACATGGCCATGCGCCTGATGGGt                                 >  3:289867/1‑149 (MQ=255)
                                                                                                             ccgccACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATCCGCAAGGGCGTCGACATGGCCATGCGCCTGATGGGTGAg                              <  4:224806/149‑1 (MQ=255)
                                                                                                             ccgccACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATCCGCAAGGGCGTCGACATGGCCATGCGCCTGATGGGTGAg                              <  1:271427/149‑1 (MQ=255)
                                                                                                                  aCCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATCCGCAAGGGCGTCGACATGGCCATGCGCCTGATGGGTGAGCAGt                          >  6:372029/1‑148 (MQ=255)
                                                                                                                       ggggCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATCCGCAAGGGCGTCGACATGGCCATGCGCCTGATGGGTGAGCAGTTCGTCa                    <  2:131073/149‑1 (MQ=255)
                                                                                                                               tgctgACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATCCGCAAGGGCGTCGACATGGCCATGCGCCTGATGGGTGAGCAGTTCGTCACCg                 <  5:141277/144‑1 (MQ=255)
                                                                                                                               tgctgACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATCCGCAAGGGCGTCGACATGGCCATGCGCCTGATGGGTGAGCAGTTCGTCACCg                 >  6:141277/1‑144 (MQ=255)
                                                                                                                               tgctgACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATCCGCAAGGGCGTCGACATGGCCATGCGCCTGATGGGTGAGCAGTTCGTCACCGGCgag            >  7:203807/1‑149 (MQ=255)
                                                                                                                                gctgACCAGCTTGCCGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATCCGCAAGGGc                                                            <  4:40966/100‑1 (MQ=255)
                                                                                                                                gctgACCAGCTTGCCGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATCCGCAAGGGc                                                            >  3:40966/1‑100 (MQ=255)
                                                                                                                                        gCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATCCGCAAGGGCGt                                                          <  4:51371/94‑1 (MQ=255)
                                                                                                                                        gCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATCCGCAAGGGCGt                                                          >  3:51371/1‑94 (MQ=255)
                                                                                                                                          aaGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATCCGCAAGGGCGTCGACATGGCCATGCGCCTGATGGGTGAGCAGTTCGTCACCGGCGAGACCATTGCCg  >  1:69646/1‑148 (MQ=255)
                                                                                                                                          |                                                                                                                                                   
CTGCTGCGTATCCCCGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATCCGCAAGGGCGTCGACATGGCCATGCGCCTGATGGGTGAGCAGTTCGTCACCGGCGAGACCATTGCCG  >  NC_002947/5630978‑5631263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑