Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,650,790 G→C 7.1% Q135E (CAG→GAG)  PP_4959 ← diguanylate cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,650,7900GC 7.1% 270.2 / 9.1 85Q135E (CAG→GAG) PP_4959diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base G (38/41);  new base C (2/4);  total (40/45)
Fisher's exact test for biased strand distribution p-value = 6.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.25e-01

GAAATGGCCGGATTGGCAAAACGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCGGACTTTTCGTTGGCGGTGAGGAAAATGATCGGGGTCAGTCGGGTA  >  NC_002947/5650642‑5650938
                                                                                                                                                    |                                                                                                                                                    
gAAATGGCCGTATTGGCAAATCGGATGATGCCATTGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGctgctg                                                                                                                                                      <  2:124673/149‑1 (MQ=255)
   aTGGCCTGATTGGCAAAACGGATGCTGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTc                                                                                                                                                   <  3:10526/149‑1 (MQ=255)
     ggCCGGATTGGCAAAACGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTc                                                                                                                                                   >  7:53983/1‑147 (MQ=255)
     ggCCGGATTGGCAAAACGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTc                                                                                                                                                   >  3:47272/1‑147 (MQ=255)
      gCCGGATTGGCAAAACGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGa                                                                                                                                                 <  8:116662/148‑1 (MQ=255)
          gATTGGCAAAACGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTCGACCagcagc                                                                                                                                            <  8:323977/149‑1 (MQ=255)
               gCAAAACGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGGTGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTCGACCAGCAgcg                                                                                                                                           >  4:59362/1‑145 (MQ=255)
               gCAAAACGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGGTGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTCGACCAGCAgcg                                                                                                                                           <  3:59362/145‑1 (MQ=255)
               gCAAAACGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTg                                                                                                                                       >  6:120797/1‑149 (MQ=255)
               gCAAAACGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTCGACCAGCAgcgcaac                                                                                                                                       <  3:54097/149‑4 (MQ=255)
                cAAAACGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCGGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTgg                                                                                                                                      <  2:156130/149‑1 (MQ=255)
                  aaaCGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCA‑TTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGAcc                                                                                                                                   >  3:129287/1‑149 (MQ=255)
                      ggATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTg                                                                                                                                <  7:345215/149‑1 (MQ=255)
                       gttgatgCCCATGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTgg                                                                                                                               >  7:40206/3‑149 (MQ=255)
                       gatgatGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTgg                                                                                                                               <  8:201766/149‑1 (MQ=255)
                       gatgatGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTgg                                                                                                                               >  3:116742/1‑149 (MQ=255)
                        ttgatgCCCATGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTggg                                                                                                                              >  3:143927/2‑149 (MQ=255)
                          gatgCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCt                                                                                                                            >  1:260638/1‑149 (MQ=255)
                           atgCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCtt                                                                                                                           <  3:362379/149‑1 (MQ=255)
                            tgCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCtt                                                                                                                           <  6:376527/148‑1 (MQ=255)
                            tgCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCtt                                                                                                                           >  5:376527/1‑148 (MQ=255)
                             gCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCtt                                                                                                                           >  1:200840/1‑147 (MQ=255)
                              ccATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTg                                                                                                                          <  2:306862/147‑1 (MQ=255)
                              ccATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTg                                                                                                                          >  1:306862/1‑147 (MQ=255)
                              ccATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGa                                                                                                                         <  2:200840/148‑1 (MQ=255)
                              ccATGCGCATCAACCACCAGAATCCCTTCAGCGGCATTTTCCAGGATGGACGCATTGAACGCCCACGCCCTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAg                                                                                                                        >  8:106094/1‑149 (MQ=255)
                               cATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAgg                                                                                                                       <  6:201555/149‑1 (MQ=255)
                                 tGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGa                                                                                                                         >  7:47269/1‑145 (MQ=255)
                                 tGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGa                                                                                                                         <  5:210850/145‑1 (MQ=255)
                                 tGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGa                                                                                                                         <  8:47269/145‑1 (MQ=255)
                                 tGGGCATCAACCACCAGAATGCCTTCCGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCCCCGGTTGCGCTGCTGCTCGAGCAGAGCCAGGACCTTCGGCTTGa                                                                                                                         >  6:210850/1‑145 (MQ=255)
                                     cATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTtgt                                                                                                                 <  4:316282/149‑1 (MQ=255)
                                     cATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTtgt                                                                                                                 <  3:94176/149‑1 (MQ=255)
                                       tCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTgg                                                                                                               <  6:135045/149‑1 (MQ=255)
                                         aaCCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTggg                                                                                                              <  2:260638/148‑1 (MQ=255)
                                         aaCCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGt                                                                                                             <  2:197794/149‑1 (MQ=255)
                                         aaCCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGt                                                                                                             >  4:311639/1‑149 (MQ=255)
                                          accaccAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTc                                                                                                            >  3:175974/1‑149 (MQ=255)
                                            caccaGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTTCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCg                                                                                                           >  4:132757/1‑148 (MQ=255)
                                            caccaGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCg                                                                                                           >  1:323018/1‑148 (MQ=255)
                                             accaGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGaa                                                                                                         >  6:298444/1‑149 (MQ=255)
                                                 gAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGt                                                                                                     <  4:116742/149‑1 (MQ=255)
                                                 gAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGt                                                                                                     <  4:175974/149‑1 (MQ=255)
                                                 gAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGt                                                                                                     >  2:73005/1‑149 (MQ=255)
                                                 gAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGt                                                                                                     <  6:41757/149‑1 (MQ=255)
                                                       cTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGaa                                                                                                >  7:178165/1‑148 (MQ=255)
                                                       cTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAAc                                                                                               <  8:261560/149‑1 (MQ=255)
                                                           ggcggcATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATg                                                                                            <  4:47272/148‑1 (MQ=255)
                                                           ggcggcA‑TTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTa                                                                                          <  4:129287/149‑1 (MQ=255)
                                                             cggcATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGa                                                                                                                         >  6:232620/1‑117 (MQ=255)
                                                             cggcATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGa                                                                                                                         <  5:232620/117‑1 (MQ=255)
                                                                    ttCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACgg                                                                                  >  2:287763/1‑149 (MQ=255)
                                                                              gACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTgg                                                                         <  3:311639/148‑1 (MQ=255)
                                                                              gACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGc                                                                        >  2:306387/1‑149 (MQ=255)
                                                                               aCGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCg                                                                       >  1:202558/1‑149 (MQ=255)
                                                                               aCGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCg                                                                       <  5:113649/149‑1 (MQ=255)
                                                                               aCGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCg                                                                       >  2:360967/1‑149 (MQ=255)
                                                                                   aTTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGc                                                                   <  5:120797/149‑1 (MQ=255)
                                                                                        aGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCttt                                                               >  8:322516/1‑148 (MQ=255)
                                                                                         ggCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGa                                                             <  2:202558/149‑1 (MQ=255)
                                                                                            ccTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCa                                                          >  4:193762/1‑149 (MQ=255)
                                                                                                   gTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGc                                                   >  4:265802/1‑149 (MQ=255)
                                                                                                        cAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTc                                                <  1:73005/147‑1 (MQ=255)
                                                                                                        cAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCg                                               <  4:143927/148‑1 (MQ=255)
                                                                                                           cTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCGGACt                                           >  6:301966/1‑149 (MQ=255)
                                                                                                            tCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCGGACtt                                          >  7:279228/1‑149 (MQ=255)
                                                                                                              aCGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCGGACtttt                                        >  8:105224/1‑149 (MQ=255)
                                                                                                                   tCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACa                                                                                              >  8:184622/1‑90 (MQ=255)
                                                                                                                     aGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCGGACTTTTCGTTgg                                  <  3:132757/148‑1 (MQ=255)
                                                                                                                     aGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCGGACTTTTCGTTg                                   <  3:193762/147‑1 (MQ=255)
                                                                                                                     aGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCGGACTTTTCGTTGGc                                 <  2:323018/149‑1 (MQ=255)
                                                                                                                       ctgctgCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCGGACTTTTCGTTGGCgg                               <  7:322516/149‑1 (MQ=255)
                                                                                                                          ctgcAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCGGACTTTTCGTTGGCGGTGa                            <  8:178165/149‑1 (MQ=255)
                                                                                                                            gcagcaTGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCGGGCTTTTCGTTGGCGGTGAg                           >  3:126969/1‑148 (MQ=255)
                                                                                                                             cagcaTGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCGGACTTTTCGTTGGCGGTGAGGa                         <  7:106094/149‑1 (MQ=255)
                                                                                                                             cagcaTGCGCCGGTTGCGAAGCTGGTAGAGCAGCGCCTGGACCTTGGGCTTGAGGATATGTGGGTCGAAAGGTTTGAACAAGTAGTCCACGGCGACGCTAGCGTAGCCTATGAGCACAGCAGCTTCGGACTTATCGTAGCCCGTGAgg                          >  2:180481/1‑148 (MQ=255)
                                                                                                                                   gcgcCGGTTGCGCTGCTGGTCGAGCAGCGCCTGACCATTGGCGTTGAGAATTT‑TTGGTGCAACGGTTTGAAAATTTAGTACAACGCCCCCCAGGCCTTCCCATTGAGGACGGCCGCATAGGACATTTTCTTTGCGGTGAGGAAAATgt                   >  6:78525/1‑147 (MQ=255)
                                                                                                                                      ccGGTTGCGCTGCTGGTCGTGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGATCATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCGGACTTTTCGTTGGCGGTGAGGAAAATGATCg                <  5:298444/149‑1 (MQ=255)
                                                                                                                                      ccGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCGGACTTTTCGTTGGCGGTGAGGAAAATGATCg                <  5:301966/149‑1 (MQ=255)
                                                                                                                                        ggTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCCCGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCGGACTTTTCGTTGGCCGTGAGGAAAATGATCggg              >  3:229507/1‑149 (MQ=255)
                                                                                                                                        ggTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCGGACTTTTCGTTGGCGGTGAGGAAAATGATCggg              <  8:53983/149‑1 (MQ=255)
                                                                                                                                          ttGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCGGGCTTTTCGTTGGCGGTGAGGAAAATGATCGGGGt            <  4:126969/149‑1 (MQ=255)
                                                                                                                                          ttGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCGGACTTTTCGTTGGCGGTGAGGAAAATGATCGGGGt            <  8:40206/149‑1 (MQ=255)
                                                                                                                                           ggcgcTGCTCGACCAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCGGACTTTTCGTTGGCGGTGa                            <  2:361828/131‑1 (MQ=255)
                                                                                                                                           ggcgcTGCTCGACCAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCGGACTTTTCGTTGGCGGTGa                            >  1:361828/2‑132 (MQ=255)
                                                                                                                                             cgcTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCGGACTTTTCGTTGGCGGTGAGGAAAATGATCGGGGTCAg         >  3:155045/1‑149 (MQ=255)
                                                                                                                                                    ggTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCGGACTTTTCGTTGGCGGTGAGGAAAATGATCGGGGTCAGTCGGGTa  >  2:198946/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GAAATGGCCGGATTGGCAAAACGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCGGACTTTTCGTTGGCGGTGAGGAAAATGATCGGGGTCAGTCGGGTA  >  NC_002947/5650642‑5650938

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑