Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,650,792 T→A 6.3% D134V (GAC→GTC)  PP_4959 ← diguanylate cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,650,7920TA 6.3% 270.2 / 5.1 88D134V (GAC→GTC) PP_4959diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base T (40/42);  new base A (2/4);  total (42/46)
Fisher's exact test for biased strand distribution p-value = 6.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.44e-01

ATGGCCGGATTGGCAAAACGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCGGACTTTTCGTTGGCGGTGAGGAAAATGATCGGGGTCAGTCGGGTAC  >  NC_002947/5650645‑5650939
                                                                                                                                                   |                                                                                                                                                   
aTGGCCTGATTGGCAAAACGGATGCTGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTc                                                                                                                                                    <  3:10526/149‑1 (MQ=255)
  ggCCGGATTGGCAAAACGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTc                                                                                                                                                    >  7:53983/1‑147 (MQ=255)
  ggCCGGATTGGCAAAACGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTc                                                                                                                                                    >  3:47272/1‑147 (MQ=255)
   gCCGGATTGGCAAAACGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGa                                                                                                                                                  <  8:116662/148‑1 (MQ=255)
       gATTGGCAAAACGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTCGACCagcagc                                                                                                                                             <  8:323977/149‑1 (MQ=255)
            gCAAAACGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGGTGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTCGACCAGCAgcg                                                                                                                                            <  3:59362/145‑1 (MQ=255)
            gCAAAACGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGGTGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTCGACCAGCAgcg                                                                                                                                            >  4:59362/1‑145 (MQ=255)
            gCAAAACGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTg                                                                                                                                        >  6:120797/1‑149 (MQ=255)
            gCAAAACGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTCGACCAGCAgcgcaac                                                                                                                                        <  3:54097/149‑4 (MQ=255)
             cAAAACGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCGGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTgg                                                                                                                                       <  2:156130/149‑1 (MQ=255)
               aaaCGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCA‑TTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGAcc                                                                                                                                    >  3:129287/1‑149 (MQ=255)
                   ggATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTg                                                                                                                                 <  7:345215/149‑1 (MQ=255)
                    gttgatgCCCATGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTgg                                                                                                                                >  7:40206/3‑149 (MQ=255)
                    gatgatGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTgg                                                                                                                                <  8:201766/149‑1 (MQ=255)
                    gatgatGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTgg                                                                                                                                >  3:116742/1‑149 (MQ=255)
                     ttgatgCCCATGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTggg                                                                                                                               >  3:143927/2‑149 (MQ=255)
                       gatgCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCt                                                                                                                             >  1:260638/1‑149 (MQ=255)
                        atgCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCtt                                                                                                                            <  3:362379/149‑1 (MQ=255)
                         tgCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCtt                                                                                                                            <  6:376527/148‑1 (MQ=255)
                         tgCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCtt                                                                                                                            >  5:376527/1‑148 (MQ=255)
                          gCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCtt                                                                                                                            >  1:200840/1‑147 (MQ=255)
                           ccATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTg                                                                                                                           <  2:306862/147‑1 (MQ=255)
                           ccATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTg                                                                                                                           >  1:306862/1‑147 (MQ=255)
                           ccATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGa                                                                                                                          <  2:200840/148‑1 (MQ=255)
                           ccATGCGCATCAACCACCAGAATCCCTTCAGCGGCATTTTCCAGGATGGACGCATTGAACGCCCACGCCCTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAg                                                                                                                         >  8:106094/1‑149 (MQ=255)
                            cATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAgg                                                                                                                        <  6:201555/149‑1 (MQ=255)
                              tGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGa                                                                                                                          <  8:47269/145‑1 (MQ=255)
                              tGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGa                                                                                                                          >  7:47269/1‑145 (MQ=255)
                              tGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGa                                                                                                                          <  5:210850/145‑1 (MQ=255)
                              tGGGCATCAACCACCAGAATGCCTTCCGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCCCCGGTTGCGCTGCTGCTCGAGCAGAGCCAGGACCTTCGGCTTGa                                                                                                                          >  6:210850/1‑145 (MQ=255)
                                  cATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTtgt                                                                                                                  <  3:94176/149‑1 (MQ=255)
                                  cATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTtgt                                                                                                                  <  4:316282/149‑1 (MQ=255)
                                    tCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTgg                                                                                                                <  6:135045/149‑1 (MQ=255)
                                      aaCCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTggg                                                                                                               <  2:260638/148‑1 (MQ=255)
                                      aaCCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGt                                                                                                              <  2:197794/149‑1 (MQ=255)
                                      aaCCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGt                                                                                                              >  4:311639/1‑149 (MQ=255)
                                       accaccAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTc                                                                                                             >  3:175974/1‑149 (MQ=255)
                                         caccaGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTTCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCg                                                                                                            >  4:132757/1‑148 (MQ=255)
                                         caccaGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCg                                                                                                            >  1:323018/1‑148 (MQ=255)
                                          accaGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGaa                                                                                                          >  6:298444/1‑149 (MQ=255)
                                              gAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGt                                                                                                      <  6:41757/149‑1 (MQ=255)
                                              gAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGt                                                                                                      <  4:175974/149‑1 (MQ=255)
                                              gAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGt                                                                                                      <  4:116742/149‑1 (MQ=255)
                                              gAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGt                                                                                                      >  2:73005/1‑149 (MQ=255)
                                                    cTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGaa                                                                                                 >  7:178165/1‑148 (MQ=255)
                                                    cTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAAc                                                                                                <  8:261560/149‑1 (MQ=255)
                                                        ggcggcATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATg                                                                                             <  4:47272/148‑1 (MQ=255)
                                                        ggcggcA‑TTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTa                                                                                           <  4:129287/149‑1 (MQ=255)
                                                          cggcATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGa                                                                                                                          >  6:232620/1‑117 (MQ=255)
                                                          cggcATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGa                                                                                                                          <  5:232620/117‑1 (MQ=255)
                                                                 ttCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACgg                                                                                   >  2:287763/1‑149 (MQ=255)
                                                                           gACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTgg                                                                          <  3:311639/148‑1 (MQ=255)
                                                                           gACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGc                                                                         >  2:306387/1‑149 (MQ=255)
                                                                            aCGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCg                                                                        <  5:113649/149‑1 (MQ=255)
                                                                            aCGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCg                                                                        >  2:360967/1‑149 (MQ=255)
                                                                            aCGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCg                                                                        >  1:202558/1‑149 (MQ=255)
                                                                                aTTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGc                                                                    <  5:120797/149‑1 (MQ=255)
                                                                                     aGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCttt                                                                >  8:322516/1‑148 (MQ=255)
                                                                                      ggCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGa                                                              <  2:202558/149‑1 (MQ=255)
                                                                                         ccTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCa                                                           >  4:193762/1‑149 (MQ=255)
                                                                                                gTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGc                                                    >  4:265802/1‑149 (MQ=255)
                                                                                                     cAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTc                                                 <  1:73005/147‑1 (MQ=255)
                                                                                                     cAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCg                                                <  4:143927/148‑1 (MQ=255)
                                                                                                        cTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCGGACt                                            >  6:301966/1‑149 (MQ=255)
                                                                                                         tCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCGGACtt                                           >  7:279228/1‑149 (MQ=255)
                                                                                                           aCGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCGGACtttt                                         >  8:105224/1‑149 (MQ=255)
                                                                                                                tCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACa                                                                                               >  8:184622/1‑90 (MQ=255)
                                                                                                                  aGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCGGACTTTTCGTTgg                                   <  3:132757/148‑1 (MQ=255)
                                                                                                                  aGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCGGACTTTTCGTTg                                    <  3:193762/147‑1 (MQ=255)
                                                                                                                  aGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCGGACTTTTCGTTGGc                                  <  2:323018/149‑1 (MQ=255)
                                                                                                                    ctgctgCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCGGACTTTTCGTTGGCgg                                <  7:322516/149‑1 (MQ=255)
                                                                                                                       ctgcAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCGGACTTTTCGTTGGCGGTGa                             <  8:178165/149‑1 (MQ=255)
                                                                                                                         gcagcaTGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCGGGCTTTTCGTTGGCGGTGAg                            >  3:126969/1‑148 (MQ=255)
                                                                                                                          cagcaTGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCGGACTTTTCGTTGGCGGTGAGGa                          <  7:106094/149‑1 (MQ=255)
                                                                                                                          cagcaTGCGCCGGTTGCGAAGCTGGTAGAGCAGCGCCTGGACCTTGGGCTTGAGGATATGTGGGTCGAAAGGTTTGAACAAGTAGTCCACGGCGACGCTAGCGTAGCCTATGAGCACAGCAGCTTCGGACTTATCGTAGCCCGTGAgg                           >  2:180481/1‑148 (MQ=255)
                                                                                                                                gcgcCGGTTGCGCTGCTGGTCGAGCAGCGCCTGACCATTGGCGTTGAGAATTT‑TTGGTGCAACGGTTTGAAAATTTAGTACAACGCCCCCCAGGCCTTCCCATTGAGGACGGCCGCATAGGACATTTTCTTTGCGGTGAGGAAAATgt                    >  6:78525/1‑147 (MQ=255)
                                                                                                                                   ccGGTTGCGCTGCTGGTCGTGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGATCATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCGGACTTTTCGTTGGCGGTGAGGAAAATGATCg                 <  5:298444/149‑1 (MQ=255)
                                                                                                                                   ccGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCGGACTTTTCGTTGGCGGTGAGGAAAATGATCg                 <  5:301966/149‑1 (MQ=255)
                                                                                                                                     ggTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCCCGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCGGACTTTTCGTTGGCCGTGAGGAAAATGATCggg               >  3:229507/1‑149 (MQ=255)
                                                                                                                                     ggTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCGGACTTTTCGTTGGCGGTGAGGAAAATGATCggg               <  8:53983/149‑1 (MQ=255)
                                                                                                                                       ttGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCGGGCTTTTCGTTGGCGGTGAGGAAAATGATCGGGGt             <  4:126969/149‑1 (MQ=255)
                                                                                                                                       ttGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCGGACTTTTCGTTGGCGGTGAGGAAAATGATCGGGGt             <  8:40206/149‑1 (MQ=255)
                                                                                                                                        ggcgcTGCTCGACCAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCGGACTTTTCGTTGGCGGTGa                             <  2:361828/131‑1 (MQ=255)
                                                                                                                                        ggcgcTGCTCGACCAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCGGACTTTTCGTTGGCGGTGa                             >  1:361828/2‑132 (MQ=255)
                                                                                                                                          cgcTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCGGACTTTTCGTTGGCGGTGAGGAAAATGATCGGGGTCAg          >  3:155045/1‑149 (MQ=255)
                                                                                                                                                 ggTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCGGACTTTTCGTTGGCGGTGAGGAAAATGATCGGGGTCAGTCGGGTa   >  2:198946/1‑149 (MQ=255)
                                                                                                                                                  gTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCGGACTTTTCGTTGGCGGTGAGGAAAATGATCGGGGTCAGTCGGGTa   <  7:105224/148‑1 (MQ=255)
                                                                                                                                                  gTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCGGACTTTTCGTTGGCGGTGAGGAAAATGATCGGGGTCAGTCGGGTAc  >  5:29659/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
ATGGCCGGATTGGCAAAACGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCGGACTTTTCGTTGGCGGTGAGGAAAATGATCGGGGTCAGTCGGGTAC  >  NC_002947/5650645‑5650939

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑