Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,651,374	T→G	6.4%	intergenic (‑182/+31)	PP_4959 ← / ← fba	diguanylate cyclase/fructose‑bisphosphate aldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,651,374	0	T	G	6.4%	237.4 / 4.6	74	intergenic (‑182/+31)	PP_4959/fba	diguanylate cyclase/fructose‑bisphosphate aldolase
Reads supporting (aligned to +/- strand):  ref base T (36/33);  new base G (3/2);  total (39/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCCATTAGTACTCTAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGCCGAAGGCTTCGTAACGC  >  NC_002947/5651234‑5651516                                                                                                                                             |                                                                                                                                               aTCCATTAGTACTCTAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCgg                                                                                                                                        >  5:131796/1‑149 (MQ=255) aTCCATTAGTACTCTAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCAGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCACATAAAAAAACCCGCATCGCTGCgg                                                                                                                                        >  5:204328/1‑149 (MQ=255)   ccATTAGTACTCTAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGt                                                                                                                                      >  6:133397/1‑149 (MQ=255)     aTTAGTACTCTAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGttt                                                                                                                                    >  1:241092/1‑149 (MQ=255)     aTTAGTACTCTAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGttt                                                                                                                                    >  4:220828/1‑149 (MQ=255)      ttAGTACTCTAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGtttt                                                                                                                                   >  7:306333/1‑149 (MQ=255)       tAGTACTCTAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCgg                                                                                                                                        <  8:232262/143‑1 (MQ=255)       tAGTACTCTAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCgg                                                                                                                                        >  7:232262/1‑143 (MQ=255)         gTACTCTAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCa                                                                                                                                <  5:133397/149‑1 (MQ=255)         gTACTCTAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCAGCGATGCGGGtttttt                                                                                                                                 <  8:306912/148‑2 (MQ=255)          tACTCTAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAg                                                                                                                               <  3:130709/149‑1 (MQ=255)           aCTCTAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAg                                                                                                                               <  6:27704/148‑1 (MQ=255)            ctctAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCa                                                                                                                                <  4:122626/146‑1 (MQ=255)            ctctAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCa                                                                                                                                >  3:122626/1‑146 (MQ=255)            ctctAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAg                                                                                                                               <  3:125210/147‑1 (MQ=255)               tAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTa                                                                                                                          >  3:202619/1‑149 (MQ=255)                aGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTAc                                                                                                                         >  4:61414/1‑149 (MQ=255)                 gACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTAcc                                                                                                                        <  1:157088/149‑1 (MQ=255)                       aGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTc                                                                                                                    <  5:75032/147‑1 (MQ=255)                             cAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAg                                                                                                              <  8:262883/147‑1 (MQ=255)                             cAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCAGCGATGCGGGtttttttatgg                                                                                                                            >  3:290544/1‑127 (MQ=255)                             cAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCAGCGATGCGGGtttttttatgg                                                                                                                            <  4:290544/133‑7 (MQ=255)                              aaCACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGtt                                                                                                            >  7:251742/1‑148 (MQ=255)                                   gcaatgcaatCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGAt                                                                                                                 <  2:118635/138‑1 (MQ=255)                                   gcaatgcaatCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGAt                                                                                                                 >  1:118635/1‑138 (MQ=255)                                   gcaatgcaatCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCtt                                                                                                      >  2:362326/1‑149 (MQ=255)                                    caatgcaatCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTg                                                                                                     <  1:362326/149‑1 (MQ=255)                                          aatCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTggcggc                                                                                                >  5:101884/1‑148 (MQ=255)                                            tCATAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTg                                                                                                     <  8:12236/141‑1 (MQ=255)                                            tCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTg                                                                                                     >  7:12236/1‑141 (MQ=255)                                            tCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAg                                                                                             <  8:251742/149‑1 (MQ=255)                                               gAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAgg                                                                                                                              >  5:15991/1‑113 (MQ=255)                                               gAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAgg                                                                                                                              <  6:15991/113‑1 (MQ=255)                                                   gCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCt                                                                                      >  1:139102/1‑149 (MQ=255)                                                   gCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCt                                                                                      >  1:19510/1‑149 (MQ=255)                                                       tCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCttt                                                                                    >  3:334874/1‑147 (MQ=255)                                                       tCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTc                                                                                   <  2:241092/148‑1 (MQ=255)                                                             aTCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAgc                                                                            >  8:183540/1‑149 (MQ=255)                                                                aaaCTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAgcgc                                                                          <  5:161048/148‑1 (MQ=255)                                                                 aaCTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTg                                                                        >  7:50184/1‑149 (MQ=255)                                                                   cTGTACGGTAGCCGACTTACGGTCGGTCTGCAGGATTTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCATGTAGCGCTGGt                                                                      <  6:204328/149‑1 (MQ=255)                                                                   cTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGt                                                                      <  6:131796/149‑1 (MQ=255)                                                                         ggTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGc                                                                >  8:103747/1‑149 (MQ=255)                                                                             gCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTc                                                            <  8:306333/149‑1 (MQ=255)                                                                                     aCGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAggg                                                                                                                             <  2:55190/76‑1 (MQ=255)                                                                                       ggtcggtcTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATc                                                  <  3:61414/149‑1 (MQ=255)                                                                                       ggtcggtcTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATc                                                  <  2:19510/149‑1 (MQ=255)                                                                                        gtcggtcTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGcc                                                               >  3:15510/1‑135 (MQ=255)                                                                                        gtcggtcTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGcc                                                               <  4:15510/135‑1 (MQ=255)                                                                                        gtcggtcTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCaga                                                        >  2:64528/1‑142 (MQ=255)                                                                                          cggtcTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGc                                               <  3:220828/149‑1 (MQ=255)                                                                                            gtcTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGGTGGTAGCTGGCTTCGAGAGAGATgggggt                                             >  4:301279/1‑146 (MQ=255)                                                                                                     aaTTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTc                                      >  1:50438/1‑147 (MQ=255)                                                                                                      aTTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAg                                   <  7:183540/149‑1 (MQ=255)                                                                                                      aTTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAg                                   <  6:101884/149‑1 (MQ=255)                                                                                                          tGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCAt                               >  5:33881/1‑149 (MQ=255)                                                                                                             aCAGGGTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTCCGAGGCATTGc                            >  7:339455/1‑149 (MQ=255)                                                                                                              cAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGcc                           >  7:346691/1‑149 (MQ=255)                                                                                                                     ccATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGt                     <  8:50184/148‑1 (MQ=255)                                                                                                                      cATCTCCCATAAAAAAACCCGCATCGCTGCGCGTATATCACGGTTACCTGACGATCACTTGACCTTGGCGGCCAGTTCACCTTTCACGTAGAGCTGGTACATGCCTTCCAGAAATATCGGATTGATATTCTCGGCATTGCCAGCTGTGc                   >  8:141796/1‑149 (MQ=255)                                                                                                                            ccATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGCCGaa               <  1:64528/147‑1 (MQ=255)                                                                                                                            ccATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGCCGAAgg             >  2:223276/1‑149 (MQ=255)                                                                                                                            ccATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGCCGAAgg             >  5:41305/1‑149 (MQ=255)                                                                                                                            ccATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGCCGAAgg             >  6:37095/1‑149 (MQ=255)                                                                                                                            ccATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGCCGAAgg             >  3:338023/1‑149 (MQ=255)                                                                                                                              aTAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGt                     >  7:196151/1‑139 (MQ=255)                                                                                                                              aTAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGt                     <  8:196151/139‑1 (MQ=255)                                                                                                                              aTAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGCCGAAGGCt           <  2:139102/149‑1 (MQ=255)                                                                                                                               tAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGCCGAAGGCtt          <  3:301279/149‑1 (MQ=255)                                                                                                                               ctgaaaaaCCCGCAGCGATGCGGGTTTTTCAGCGTTACCTGTCGATAAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGAACGGCTTGATCTACGAGGCATTGCCCGCGGTGCCGAAGGCtt          >  8:90620/4‑149 (MQ=255)                                                                                                                                tgaaaaaCCCGCAGCGATGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGATCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGCCGAAGGCTTc         >  5:73397/3‑149 (MQ=255)                                                                                                                                       cccGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGCCGAAGGCTTCGTAAcgc  <  4:334874/149‑1 (MQ=255)                                                                                                                                          gCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAg                                   >  8:229162/1‑113 (MQ=255)                                                                                                                                          gCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAg                                   <  7:229162/113‑1 (MQ=255)                                                                                                                                             |                                                                                                                                               ATCCATTAGTACTCTAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGCCGAAGGCTTCGTAACGC  >  NC_002947/5651234‑5651516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑