Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,699,749 A→G 6.5% D76G (GAC→GGC)  phaA → poly(3‑hydroxyalkanoate) polymerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,699,7490AG 6.5% 173.9 / 2.3 63D76G (GAC→GGC) phaApoly(3‑hydroxyalkanoate) polymerase
Reads supporting (aligned to +/- strand):  ref base A (30/27);  new base G (2/2);  total (33/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.94e-01

AGCTCGGCACGCACCGTGCTGCGCCAGGCCGTGCGCCAACCGCTGCACAGCGCCAAGCATGTGGCTCACTTTGGCCTGGAGCTGAAGAACGTGTTGCTGGGCAAATCCAGCCTGGCCCCGGACAGCGACGACCGTCGCTTCAATGACCCGGCCTGGAGCAACAACCCGCTGTACCGCCGCTACCTGCAAACCTACCTGGCCTGGCGCAAGGAGCTGCAGGACTGGGTGAGCAGCAGCGACCTGTCCCCCCAGGACATCAGCCGCGGCCAGTTCGTCA  >  NC_002947/5699619‑5699895
                                                                                                                                  |                                                                                                                                                  
aGCTCGGCACGCACCGTGCTGCGCCAGGCCGTGCGCCAACCGCTGCACAGCGCCAAGCATGTGGCTCACTTTGGCCTGGAGCTGAAGAACGTGTTGCTGGGCAAATCCAGCCTGGCCCCGGACAGCGACGACCGTCGCTTCAATGAccc                                                                                                                                  >  7:198843/1‑149 (MQ=255)
   tCGGCACGCACCGTGCTGCGCCAGGCCGTGCGCCAACCGCTGCACAGCGCCAAGCATGTGGCTCACTTTGGCCTGGAGCTGAAGAACGTGTTGCTGGGCAAATCCAGCCTGGCCCCGGACAGCGACGACCGTCGCTTCAATGACCCGGc                                                                                                                               >  8:172248/1‑149 (MQ=255)
   tCGGCACGCACCGTGCTGCGCCAGGCCGTGCGCCAACCGCTGCACAGCGCCAAGCATGTGGCTCACTTTGGCCTGGAGCTGAAGAACGTGTTGCTGGGCAAATCCAGCCTGGCCCCGGACAGCGACGACCGTCGCTTCAATGACCCGGc                                                                                                                               >  2:322893/1‑149 (MQ=255)
       cacgcacCGTGCTGCGCCAGGCCGTGCGCCAACCGCTGCACAGCGCCAAGCATGTGGCTCACTTTGGCCTGGAGCTGAAGAACGTGTTGCTGGGCAAATCCAGCCTGGCCCCGGACAGCGACGACCGTCGCTTCAATGACCCGGCCTg                                                                                                                            >  5:128637/1‑148 (MQ=255)
          gcacCGTGCTGCGCCAGGCCGTGCGCCAACCGCTGCACAGCGCCAAGCATGTGGCTCACTTTGGCCTGGAGCTGAAGAACGTGTTGCTGGGCAAATCCAGCCTGGCCCCGGACAGCGACGACCGTCGCTTCAATGACCCGGCCTGGAGc                                                                                                                        <  2:236238/149‑1 (MQ=255)
           cacCGTGCTGCGCCAGGCCGTGCGCCAACCGCTGCACAGCGCCAAGCATGTGGCTCACTTTGGCCTGGAGCTGAAGAACGTGTTGCTGGGCAAATCCAGCCTGGCCCCGGACAGCGACGACCGTCGCTTCaa                                                                                                                                        <  8:341428/132‑1 (MQ=255)
           cacCGTGCTGCGCCAGGCCGTGCGCCAACCGCTGCACAGCGCCAAGCATGTGGCTCACTTTGGCCTGGAGCTGAAGAACGTGTTGCTGGGCAAATCCAGCCTGGCCCCGGACAGCGACGACCGTCGCTTCaa                                                                                                                                        >  7:341428/1‑132 (MQ=255)
             ccGTGCTGCGCCAGGCCGTGCGCCAACCGCTGCACAGCGCCAAGCATGTGGCTCACTTTGGCCTGGAGCTGAAGAACGTGTTGCTGGGCAAATCCAGCCTGGCCCCGGACAGCGACGACCGTCGCTTCAATGACCCGGCCTGGAGcaac                                                                                                                     <  3:213826/149‑1 (MQ=255)
                tgctgcGCCAGGCCGTGCGCCAACCGCTGCACAGCGCCAAGCATGTGGCTCACTTTGGCCTGGAGCTGAAGAACGTGTTGCTGGGCAAATCCAGCCTGGCCCCGGACAGCGACGACCGTCGCTTCAATGACCCGGCCTGGAGCaacaac                                                                                                                  <  7:172248/149‑1 (MQ=255)
                         aGGCCGTGCGCCAACCGCTGCACAGCGCCAAGCATGTGGCTCACTTTGGCCTGGAGCTGAAGAACGTGTTGCTGGGCAAATCCAGCCTGGCCCCGGACAGCGACGACCGTCGCTTCAATGACCCGGCCTGGAGCAACAACCCGCTGTAc                                                                                                         <  3:300586/149‑1 (MQ=255)
                           gCCGTGCGCCAACCGCTGCACAGCGCCAAGCATGTGGCTCACTTTGGCCTGGAGCTGAAGAACGTGTTGCTGGGCAAATCCAGCCTGGCCCCGGACAGCGACGACCGTCGCTTCAATGACCCGGCCTGGAGCAACAACCCGCTGTAccg                                                                                                       >  5:203142/1‑149 (MQ=255)
                             cGTGCGCCAACCGCTGCACAGCGCCAAGCATGTGGCTCACTTTGGCCTGGAGCTGAAGAACGTGTTGCTGGGCAAATCCAGCCTGGCCCCGGACAGCGACGACCGTCGCTTCAATGACCCGGCCTGGAGCAACAACCCGCTGTAccgcc                                                                                                     >  4:273845/1‑149 (MQ=255)
                               tGCGCCAACCGCTGCACAGCGCCAAGCATGTGGCTCACTTTGGCCTGGAGCTGAAGAACGTGTTGCTGGGCAAATCCAGCCTGGCCCCGGACAGCGACGACCGTCGCTTCAATGACCCGGCCTGGAGCAACAACCCGCTGTACcgccgc                                                                                                   <  6:128637/149‑1 (MQ=255)
                                 cgcCAACCGCTGCACAGCGCCAAGCATGTGGCTCACTTTGGCCTGGAGCTGAAGAACGTGTTGCTGGGCAAATCCAGCCTGGCCCCGGACAGCGACGACCGTCGCTTCAATGACCCGGCCTGGAGCaacaac                                                                                                                  >  4:92207/1‑132 (MQ=255)
                                 cgcCAACCGCTGCACAGCGCCAAGCATGTGGCTCACTTTGGCCTGGAGCTGAAGAACGTGTTGCTGGGCAAATCCAGCCTGGCCCCGGACAGCGACGACCGTCGCTTCAATGACCCGGCCTGGAGCaacaac                                                                                                                  <  3:92207/132‑1 (MQ=255)
                                        cGCTGCACAGCGCCAAGCATGTGGCTCACTTTGGCCTGGAGCTGAAGAACGTGTTGCTGGGCAAATCCAGCCTGGCCCCGGACAGCGACGACCGTCGCTTCAATGACCCGGCCTGGAGCAACAACCCGCTGTACCGCCGCTACCTGCaa                                                                                          <  3:273845/149‑1 (MQ=255)
                                        cGCTGCACAGCGCCAAGCATGTGGCTCACTTTGGCCTGGAGCTGAAGAACGTGTTGCTGGGCAAATCCAGCCTGGCCCCGGACAGCGACGACCGTCGCTTCAATGACCCGGCCTGGAGCAACAACCCGCTGTACCGCCGCTACCTGCaa                                                                                          <  6:203142/149‑1 (MQ=255)
                                          cTGCACAGCGCCAAGCATGTGGCTCACTTTGGCCTGGAGCTGAAGAACGTGTTGCTGGGCAAATCCAGCCTGGCCCCGGACAGCGACGACCGTCGCTTCAATGACCCGGCCTGGAGCAACAACCCGCTGTACCGCCGCTACCTGCaaa                                                                                         >  6:31072/1‑148 (MQ=255)
                                             cacaGCGCCAAGCATGTGGCTCACGTTGGCCTGGAGCTGAAGCACGTGTGGCTGGGCAAATCCAGCCTGTCCCCGGACAGCGACTCCCGTCGCTTCAATGACCCGGCCTGGAGCAACAACCCGCTGGACCGCCGCTACCTTCATacct                                                                                      <  7:244363/148‑1 (MQ=255)
                                                     cAAGCATGTGGCTCACTTTGGCCTGGAGCTGAAGAACGTGTTGCTGGGCAAATCCAGCCTGGCCCCGGACAGCGACGACCGTCGCTTCAATGACCCGGCCTGGAGCAACAACCCGCTGTACCGCCGCTACCTGCAAACCTAcctggcct                                                                             >  6:304826/1‑149 (MQ=255)
                                                     cAAGCATGTGGCTCACTTTGGCCTGGAGCTGAAGAACGTGTTGCTGGGCAAATCCAGCCTGGCCCCGGACAGCGACGACCGTCGCTTCAATGACCCGGCCTGGAGCAACAACCCGCTGTACCGCCGCTACCTGCAAACCTAcctggcct                                                                             >  4:275963/1‑149 (MQ=255)
                                                      aaGCATGTGGCTCACTTTGGCCTGGAGCTGAAGAACGTGTTGCTGGGCAAATCCAGCCTGGCCCCGGACAGCGACGACCGTCGCTTCAATGACCCGGCCTGGAGCAACAACCCGCTGTACCGCCGCTACCTGCAAACCTAcctggcctg                                                                            >  4:188320/1‑149 (MQ=255)
                                                       aGCATGTGGCTCACTTTGGCCTGGAGCTGAAGAACGTGTTGCTGGGCAAATCCAGCCTGGCCCCGGACAGCGACGACCGTCGCTTCAATGACCCGGCCTGGAGCAACAACCCGCTGTACCGCCGCTACCTGCAAACCTAcctggcctgg                                                                           >  6:364492/1‑149 (MQ=255)
                                                        gCATGTGGCTCACTTTGGCCTGGAGCTGAAGAACGTGTTGCTGGGCAAATCCAGCCTGGCCCCGGACAGCGACGACCGTCGCTTCAATGACCCGGCCTGGAGCAACAACCCGCTGTACCGCCGCTACCTGCAAACCTACctggcctggc                                                                          <  5:31072/149‑1 (MQ=255)
                                                            gtgGCTCACTTTGGCCTGGAGCTGAAGAACGTGTTGCTGGGCAAATCCAGCCTGGCCCCGGACAGCGACGACCGTCGCTTCAATGACCCGGCCTGGAGCAACAACCCGCTGTACCGCCGCTACCTGCAAACCTACCTGGCCTGgcgc                                                                        >  2:61125/1‑147 (MQ=255)
                                                             tgGCTCACTTTGGCCTGGAGCTGAAGAACGTGTTGCTGGGCAAATCCAGCCTGGCCCCGGACAGCGACGACCGTCGCTTCAATGACCCGGCCTGGAGCAACAACCCGCTGTACCGCCGCTACCTGCAAACCTACCTGGCCTGGCGCAAg                                                                     <  1:322893/149‑1 (MQ=255)
                                                                cTCACTTTGGCCTGGAGCTGAAGAACGTGTTGCTGGGCAAATCCAGCCTGGCCCCGGACAGCGACGACCGTCGCTTCAATGACCCGGCCTGGAGCAACAACCCGCTGTACCGCCGCTACCTGCAAACCTACCTGGCCTGGCGCAAGGAg                                                                  <  3:275963/149‑1 (MQ=255)
                                                                 tCACTTTGGCCTGGAGCTGAAGAACGTGTTGCTGGGCAAATCCAGCCTGGCCCCGGACAGCGACGACCGTCGCTTCAATGACCCGGCCTGGAGCAACAACCCGCTGTACCGCCGCt                                                                                                  >  2:225223/1‑116 (MQ=255)
                                                                 tCACTTTGGCCTGGAGCTGAAGAACGTGTTGCTGGGCAAATCCAGCCTGGCCCCGGACAGCGACGACCGTCGCTTCAATGACCCGGCCTGGAGCAACAACCCGCTGTACCGCCGCt                                                                                                  <  1:225223/116‑1 (MQ=255)
                                                                 tCACTTTGGCCTGGAGCTGAAGAACGTGTTGCTGGGCAAATCCAGCCTGGCCCCGGACAGCGACGACCGTCGCTTCAATGACCCGGCCTGGAGCAACAACCCGCTGTACCGCCGCt                                                                                                  >  8:127104/1‑116 (MQ=255)
                                                                 tCACTTTGGCCTGGAGCTGAAGAACGTGTTGCTGGGCAAATCCAGCCTGGCCCCGGACAGCGACGACCGTCGCTTCAATGACCCGGCCTGGAGCAACAACCCGCTGTACCGCCGCt                                                                                                  <  7:127104/116‑1 (MQ=255)
                                                                 tCACTTTGGCCTGGAGCTGAAGAACGTGTTGCTGGGCAAATCCAGCCTGGCCCCGGACAGCGACGACCGTCGCTTCAATGACCCGGCCTGGAGCAACAACCCGCTGTACCGCCGCTACCTGCAAACCTACCTGGCCTGGCGCAAGGAGc                                                                 >  6:110827/1‑149 (MQ=255)
                                                                    cTTTGGCCTGGAGCTGAAGAACGTGTTGCTGGGCAAATCCAGCCTGGCCCCGGACAGCGACGACCGTCGCTTCAATGACCCGGCCTGGAGCAACAACCCGCTGTACCGCCGCTACCTGCAAACCTACCTGGCCTGGCGCAAGGAGCTGc                                                              <  5:201650/149‑1 (MQ=255)
                                                                     tttGGCCTGGAGCTGAAGAACGTGTTGCTGGGCAAATCCAGCCTGGCCCCGGACAGCGACGACCGTCGCTTCAATGACCCGGCCTGGAGCAACAACCCGCTGTACCGCCGCTACCTGCAAACCTACCTGGCCTGGCGCAAGGAGCTg                                                               >  5:213220/1‑147 (MQ=255)
                                                                        ggCCTGGAGCTGAAGAACGTGTTGCTGGGCAAATCCAGCCTGGCCCCGGACAGCGACGACCGTCGCTTCAATGACCCGGCCTGGAGCAACAACCCGCTGTACCGCCGCTACCTGCAAACCTACCTGGCCTGGCGCAAGGAGCTGCAGGa                                                          >  6:270066/1‑149 (MQ=255)
                                                                           cTGGAGCTGAAGAACGTGTTGCTGGGCAAATCCAGCCTGGCCCCGGACAGCGACGACCGTCGCTTCAATGACCCGGCCTGGAGCAACAACCCGCTGTACCGCCGCTACCTGCAAACCTACCTGGCCTGGCGCAAGGAGCTGCAGGACTg                                                       >  1:201026/1‑149 (MQ=255)
                                                                            tGGAGCTGAAGAACGTGTTGCTGGGCAAATCCAGCCTGGCCCCGGACAGCGACGACCGTCGCTTCAATGACCCGGCCTGGAGCAACAACCCGCTGTACCGCCGCTACCTGCAAACCTACCTGGCCTGGCGCAAGGAGCTGCAGGACTgg                                                      >  5:135240/1‑149 (MQ=255)
                                                                              gAGCTGAAGAACGTGTTGCTGGGCAAATCCAGCCTGGCCCCGGACAGCGACGACCGTCGCTTCAATGACCCGGCCTGGAGCAACAACCCGCTGTACCGCCGCTACCTGCAAACCTACCTGGCCTGGCGCAAGGAGCTGCAGGACTGGGt                                                    <  3:196667/149‑1 (MQ=255)
                                                                                gCTGAAGAACGTGTTGCTGGGCAAATCCAGCCTGGCCCCGGACAGCGACGACCGTCGCTTCAATGACCCGGCCTGGAGCAACAACCCGCTGTACCGCCGCTACCTGCAAACCTACCTGGCCTGGCGCAAGGAGCTGCAGGACTGGGTGa                                                  <  5:364492/149‑1 (MQ=255)
                                                                                gCTGAAGAACGTGTTGCTGGGCAAATCCAGCCTGGCCCCGGACAGCGACGACCGTCGCTTCAATGACCCGGCCTGGAGCAACAACCCGCTGTACCGCCGCTACCTGCAAACCTACCTGGCCTGGCGCAAGGAGCTGCAGGACTGGGTGa                                                  <  2:201026/149‑1 (MQ=255)
                                                                                 cTGAAGAACGTGTTGCTGGGCAAATCCAGCCTGGCCCCGGACAGCGACGACCGTCGCTTCAATGACCCGGCCTGGAGCAACAACCCGCTGTACCGCCGCTACCTGCAAACCTACCTGGCCTGGCGCAAGGAGCTGCAGGACTGGGTGag                                                 <  5:270066/149‑1 (MQ=255)
                                                                                    aagaaCGTGTTGCTGGGCAAATCCAGCCTGGCCCCGGACAGCGACGACCGTCGCTTCAATGACCCGGCCTGGAGCAACAACCCGCTGTACCGCCGCTACCTGCAAACCTACCTGGCCTGGCGCAAGGAGCTGCAGGACTGGGTGagcag                                              >  5:52212/1‑149 (MQ=255)
                                                                                    aagaaCGTGTTGCTGGGCAAATCCAGCCTGGCCCCGGACAGCGACGACCGTCGCTTCAATGACCCGGCCTGGAGCAACAACCCGCTGTACCGCCGCTACCTGCAAACCTACCTGGCCTGGCGCAAGGAGCTGCAGGACTGGGTGagc                                                <  5:304826/147‑1 (MQ=255)
                                                                                    aagaaCGTGTTGCTGGGCAAATCCAGCCTGGCCCCGGACAGCGACGACCGTCGCTTCAATGACCCGGCCTGGAGCAACAACCCGCTGTACCGCCGCTACCTGCAAACCTACCTGGCCTGGCGCAAGGAGCTGCAGGACTGGGTGagc                                                <  3:77964/147‑1 (MQ=255)
                                                                                        aCGTGTTGCTGGGCAAATCCAGCCTGGCCCCGGACAGCGACGACCGTCGCTTCAATGACCCGGCCTGGAGCAACAACCCGCTGTACCGCCGCTACCTGCAAACCTACCTGGCCTGGCGCAAGGAGCTGCAGGACTGGGTGagcagcagc                                          <  4:200615/149‑1 (MQ=255)
                                                                                         cGTGTTGCTGGGCAAATCCAGCCTGGCCCCGGACAGCGACGACCGTCGCTTCAATGACCCGGCCTGGAGCAACAACCCGCTGTACCGCCGCTACCTGCAAACCTACCTGGCCTGGCGCAAGGAGCTGCAGGACt                                                        <  4:142049/134‑1 (MQ=255)
                                                                                         cGTGTTGCTGGGCAAATCCAGCCTGGCCCCGGACAGCGACGACCGTCGCTTCAATGACCCGGCCTGGAGCAACAACCCGCTGTACCGCCGCTACCTGCAAACCTACCTGGCCTGGCGCAAGGAGCTGCAGGACt                                                        >  3:142049/1‑134 (MQ=255)
                                                                                           tgtTGCTGGGCAAATCCAGCCTGGCCCCGGACAGCGACGACCGTCGCTTCAATGACCCGGCCTGGAGCAACAACCCGCTGTACCGCCGCTACCTGCAAACCTACCTGGCCTGGCGCAAGGAGCTGCAGGACTGGGTGAGCAGCAGCGAc                                       <  6:52212/149‑1 (MQ=255)
                                                                                             ttGCTGGGCAAATCCAGCCTGGCCCCGGACAGCGACGACCGTCGCTTCAATGACCCGGCCTGGAGCAACAACCCGCTGTACCGCCGCTACCTGCAAACCTACCTGGCCTGGCGCAAGGAGCTGCAGGACTGGGTGAGCAGCAGCGACCt                                     >  5:269267/1‑149 (MQ=255)
                                                                                               gCTGGGCAAATCCAGCCTGGCCCCGGACAGCGACGCCCGTCGCTTCAATGACCCGGCCTGGAGCAACAACCCGCTGTACCGCCGCTACCTGCAAACCTACCTGGCCTGGCGCAAGGAGCTGCAGGACTGGGTGAGCAGCAGCGACCTGt                                   >  8:52777/1‑149 (MQ=255)
                                                                                               gCTGGGCAAATCCAGCCTGGCCCCGGACAGCGACGACCGTCGCTTCAATGACCCGGCCTGGAGCAACAACCCGCTGTACCGCCGCTACCTGCAAACCTACCTGGCCTGGCGCAAGGAGCTGCAGGACTGGGTGAGCAGCAGCGACCTg                                    <  6:213220/148‑1 (MQ=255)
                                                                                                   ggCAAATCCAGCCTGGCCCCGGACAGCGACGACCGTCGCTTCAATGACCCGGCCTAGAGCAACAACCCGCTGTACCGCCGCTACCTGCAAACCTACCTGGCCTGGCGCAAGGAGCTGCAGGACTGGGTGAGCAGCAGCGACCTGTcccc                               >  2:163998/1‑149 (MQ=255)
                                                                                                      aaaTCCAGCCTGGCCCCGGACAGCGACGACCGTCGCTTCAATGACCCGGCCTGGAGCAACAACCCGCTGTACCGCCGCTACCt                                                                                              >  2:220974/1‑83 (MQ=255)
                                                                                                      aaaTCCAGCCTGGCCCCGGACAGCGACGACCGTCGCTTCAATGACCCGGCCTGGAGCAACAACCCGCTGTACCGCCGCTACCt                                                                                              <  1:220974/83‑1 (MQ=255)
                                                                                                           cAGCCTGGCCCCGGACAGCGACGACCGTCGCTTCAATGACCCGGCCTGGAGCAACAACCCGCTGTACCGCCGCTACCTGCAAACCTACCTGGCCTGGCGCAAGGAGCTGCAGGACTGGGTGAGCAGCAGCGACCTGTCCCCCCAGGACa                       >  7:42635/1‑149 (MQ=255)
                                                                                                            aGCCTGGCCCCGGACAGCGACGACCGTCGCTTCAATGACCCGGCCTGGAGCAACAACCCGCTGTACCGCCGCTACCTGCAAACCTACCTGGCCTGGCGCAAGGAGCTGCAGGACTGGGTGAGCAGCAGCGACCTGTCCCCCCAGGACAt                      >  5:358350/1‑149 (MQ=255)
                                                                                                            aGCCTGGCCCCGGACAGCGACGACCGTCGCTTCAATGACCCGGCCTGGAGCAACAACCCGCTGTACCGCCGCTACCTGCAAACCTACCTGGCCTGGCGCAAGGAGCTGCAGCACTGGGTGAGCAGCAGCGACCTGTCCCCCCAGGACa                       >  4:68253/1‑148 (MQ=255)
                                                                                                             gCCTGGCCCCGGACAGCGACGACCGTCGCTTCAATGACCCGGCCTGGAGCAACAACCCGCTGTACCGCCGCTACCTGCAAACCTACCTGGCCTGGCGCAAGGAGCTGCAGGACTGGGTGAGCAGCAGCGACCTGTCCCCCCAGGACAt                      >  6:197804/1‑148 (MQ=255)
                                                                                                                       ttgaaGCGACGGTCGTCGCTTCAATGACCCGGCCTGGAGCAACAACCCGCTGTACCGCCGCTACCTGCAAACCTACCTGGCCTGGCGCAAGGAGCTGCAGGACTGGGTGAGCAGCAGCGACCTGTCCCCCCAg                           <  2:347516/129‑1 (MQ=255)
                                                                                                                       ttgaaGCGACGGTCGTCGCTTCAATGACCCGGCCTGGAGCAACAACCCGCTGTACCGCCGCTACCTGCAAACCTACCTGGCCTGGCGCAAGGAGCTGCAGGACTGGGTGAGCAGCAGCGACCTGTCCCCCCAg                           >  1:347516/5‑133 (MQ=255)
                                                                                                                        tgaaGCGACGGTCGTCGCTTCAATGACCCGGCCTGGAGCAACAACCCGCTGTACCGCCGCTACCTGCAAACCTACCTGGCCTGGCGCAAGGAGCTGCAGGACTGGGTGAGCAGCAGCGACCTGTCCCCCCAGGACATCAGc                  >  7:137652/4‑141 (MQ=255)
                                                                                                                        tgaaGCGACGGTCGTCGCTTCAATGACCCGGCCTGGAGCAACAACCCGCTGTACCGCCGCTACCTGCAAACCTACCTGGCCTGGCGCAAGGAGCTGCAGGACTGGGTGAGCAGCAGCGACCTGTCCCCCCAGGACATCAGc                  <  8:137652/138‑1 (MQ=255)
                                                                                                                        gACAGCGACGACCGTCGCTTCAATGACCCGGCCTGGAGCAACAACCCGCTGTACCGCCGCTACCTGCCAACCTACCTGGCCTGGCGCAAGGAGCTGCAGGACTGGGTGAGCAGCAGCGACCTGTCCCCCCAGGACATCAGCCGCGGCCa          <  5:110827/149‑1 (MQ=255)
                                                                                                                              gacgacCGTCGCTTCAATGACCCGGCCTGGAGCAACAACCCGCTGTACCGCCGCTACCTGCAAACCTACCTGGCCTGGCGCAAGGAGCTGCAGGACTGGGTGAGCAGCAGCGACCTGTCCCCCCAGGACATCAGCCGCGGCCAGTTCGt    <  1:61125/149‑1 (MQ=255)
                                                                                                                                cgacCGTCGCTTCAATGACCCGGCCTGGAGCAACAACCCGCTGTACCGCCGCTACCTGCAAACCTACCTGGCCTGGCGCAAGGAGCTGCAGGACTGGGTGAGCAGCAGCGACCTGTCCCCCCAGGACATCAGCCGCGGCCAGTTCGtca  <  8:198843/149‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                                  
AGCTCGGCACGCACCGTGCTGCGCCAGGCCGTGCGCCAACCGCTGCACAGCGCCAAGCATGTGGCTCACTTTGGCCTGGAGCTGAAGAACGTGTTGCTGGGCAAATCCAGCCTGGCCCCGGACAGCGACGACCGTCGCTTCAATGACCCGGCCTGGAGCAACAACCCGCTGTACCGCCGCTACCTGCAAACCTACCTGGCCTGGCGCAAGGAGCTGCAGGACTGGGTGAGCAGCAGCGACCTGTCCCCCCAGGACATCAGCCGCGGCCAGTTCGTCA  >  NC_002947/5699619‑5699895

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑