Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 495,081 T→A 6.4% E318V (GAG→GTG)  PP_0408 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947495,0810TA 6.4% 183.5 / 3.0 62E318V (GAG→GTG) PP_0408hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (30/28);  new base A (2/2);  total (32/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.64e-01

GTGCGTGAAGCAACCGCGCGTACCCGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATTGTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGCCGTTGCTGGGCTGCGTTGCGGTCAGCGCTGCGGGTGGCGTAGTAGATATCGGCCGTGGGTAC  >  NC_002947/494933‑495229
                                                                                                                                                    |                                                                                                                                                    
gTGCGTGAAGCAACCGCGCGTACCCGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGct                                                                                                                                                      <  5:201803/149‑1 (MQ=255)
       aaGCAACCGCGCGTACCCGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTc                                                                                                                                               >  8:157899/1‑149 (MQ=255)
       aaGCAACCGCGCGTACCCGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAAGAGCAATTGTTc                                                                                                                                               >  3:147151/1‑149 (MQ=255)
              cgcgcgTACGCGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAAGAGCAATTGTTccgccggg                                                                                                                                        <  4:147151/149‑3 (MQ=255)
                         gTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCtgg                                                                                                                             >  3:147122/1‑149 (MQ=255)
                            aGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCtggcat                                                                                                                          <  8:158716/149‑1 (MQ=255)
                             gTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCtggcatg                                                                                                                         >  4:124119/1‑149 (MQ=255)
                             gTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCtg                                                                                                                              <  1:93865/144‑1 (MQ=255)
                             gTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCtg                                                                                                                              >  2:93865/1‑144 (MQ=255)
                                  aCCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGcaat                                                                                                                    <  6:233811/149‑1 (MQ=255)
                                         cTATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGAc                                                                                                             <  7:157899/149‑1 (MQ=255)
                                          tATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGctc                                                                                                                                                     >  7:294443/1‑108 (MQ=255)
                                          tATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGctc                                                                                                                                                     <  8:294443/108‑1 (MQ=255)
                                          tATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGAc                                                                                                             >  1:193154/1‑148 (MQ=255)
                                           aTGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACaa                                                                                                           >  8:76151/1‑149 (MQ=255)
                                                 tGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATTGTCg                                                                                                     >  4:220834/1‑149 (MQ=255)
                                                   aTCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATTGTCgg                                                                                                    <  8:191578/148‑1 (MQ=255)
                                                    tCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCtggcatgg                                                                                                                        >  5:374712/1‑127 (MQ=255)
                                                    tCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCtggcatgg                                                                                                                        <  6:374712/127‑1 (MQ=255)
                                                       gCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATTGTCGGTAATg                                                                                               >  5:262626/1‑149 (MQ=255)
                                                        cAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATTGTCGGTACTgg                                                                                              <  5:47869/149‑1 (MQ=255)
                                                         aaGCGGCGCGGCCTTTGACGCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATTGTCGGTACTGGc                                                                                             >  2:250911/1‑149 (MQ=255)
                                                           gcggcgcggcCTTTGACGCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATTGTCGGTACTgg                                                                                              <  8:313316/146‑1 (MQ=255)
                                                           gcggcgcggcCTTTGACGCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATTGTCGGTACTGGcag                                                                                           >  7:313316/1‑147 (MQ=255)
                                                                gcggcCTTTGACGCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATTGTCGGTACTGGCTATCCt                                                                                       >  8:14712/1‑148 (MQ=255)
                                                                  ggcCTTTGACGCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATTGTCGGTACt                                                                                                >  4:114657/1‑137 (MQ=255)
                                                                  ggcCTTTGACGCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATTGTCGGTACt                                                                                                <  3:114657/137‑1 (MQ=255)
                                                                      tttGACGCCCCGCCACAGGGATCAACCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCATGTTCCGCCGCTATGTTTGCTGGCATGGCAATCAATGACAATTGTCGGTACAGGCAATCCATCTGCCg                                                                                >  8:155654/1‑149 (MQ=255)
                                                                          aCGCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATTGTCGGTACTGGCTATCCTTCTGCCGTTTg                                                                            >  4:260010/1‑149 (MQ=255)
                                                                           cGCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATTGTCGGTACTGGCTATCCTTCTGCCGTTTGc                                                                           <  7:14712/149‑1 (MQ=255)
                                                                                 gCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATTGTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCt                                                                     <  4:215399/149‑1 (MQ=255)
                                                                                     caGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATAAATGACAATTTTCGGTACTGGCAATCATTCTGCCGTTTGCTGGCATGCAg                                                                 >  6:26707/1‑149 (MQ=255)
                                                                                      aGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGTCAATCAATGACAATTGTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGc                                                                >  6:287683/1‑149 (MQ=255)
                                                                                      aGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATTGTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGc                                                                >  4:246656/1‑149 (MQ=255)
                                                                                      aGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCA‑CCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATTGTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGcc                                                               >  3:298459/1‑149 (MQ=255)
                                                                                      aGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCA‑CCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATTGTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGcc                                                               >  3:293436/1‑149 (MQ=255)
                                                                                              aGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATTGTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGCCGTTGCTg                                                        <  2:193154/149‑1 (MQ=255)
                                                                                               gCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATTGTCGGTACTgg                                                                                              >  6:379592/1‑110 (MQ=255)
                                                                                               gCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATTGTCGGTACTgg                                                                                              <  5:379592/110‑1 (MQ=255)
                                                                                                       cAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATTGTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCCGCAGCCGTTGCTGGGCTGCGtt                                               >  4:131507/1‑149 (MQ=255)
                                                                                                        aGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATTGTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGCCGTTGCTGGGCTGCGTTg                                              <  2:255786/149‑1 (MQ=255)
                                                                                                          gccTGAGGGCTCATCCACCCCCTCACCCTGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATTGTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGCCGTTGCTGGGCTGCGTTGCg                                            <  5:26707/148‑1 (MQ=255)
                                                                                                          cccTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATTGTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGCCGTTGCTGGGCTGCGTTGCg                                            <  3:260010/149‑1 (MQ=255)
                                                                                                          cccTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATTGTCGGTAATGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGCCGTTGCTGGGCTGCGTTGCg                                            <  6:262626/149‑1 (MQ=255)
                                                                                                           ccTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATTGTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTCCAGCCGTTGCTGGGCTGCGTTGCgg                                           >  4:281095/1‑149 (MQ=255)
                                                                                                            cTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATTGTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGCCGTTGCTGGGCTGCGTTGCGGt                                          >  8:92642/1‑149 (MQ=255)
                                                                                                                    tCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATTGTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGCCGTTGCTGGGCTGCGTTGCGGTCAGCGCTg                                  >  1:94768/1‑149 (MQ=255)
                                                                                                                      aTCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATTGTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGCCGTTGCTGGGCTGCGTTGCGGTCAGCGCTGc                                 <  1:40649/148‑1 (MQ=255)
                                                                                                                      aTCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATTGTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGCCGTTGCTGGGCTGCGTTGCGGTCAGCGCTGCg                                >  5:108343/1‑149 (MQ=255)
                                                                                                                      aTCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATTGTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGCCGTTGCTGGGCTGCGTTGCGGTCAGCGCTGCg                                >  2:171873/1‑149 (MQ=255)
                                                                                                                      aTCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGAAATCAATGACAATTGTAGGTACTGGCTATCCTTCTTCCGTTTGCTGGCCTGCAGCCTTTTATGGGCTGCGTTGCGGTCAGAGCTGCg                                >  2:177535/1‑149 (MQ=255)
                                                                                                                         cACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATTGTCGGTACTGGCTATCCTTCTGCCGTTTGCTg                                                                         <  3:197469/105‑1 (MQ=255)
                                                                                                                         cACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATTGTCGGTACTGGCTATCCTTCTGCCGTTTGCTg                                                                         >  4:197469/1‑105 (MQ=255)
                                                                                                                          aCCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATTGTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCCGCAGCCGTTGCTGGGCTGCGTTGCGGTCAGCGCTGCGGGTg                            <  3:131507/149‑1 (MQ=255)
                                                                                                                                gCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATTGTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGCCGTTGCTGGGCTGCGTTGCGGTCAGCGCTGCGGGTGGCgtag                      >  4:215259/1‑149 (MQ=255)
                                                                                                                                 cACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATTGTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGCCGTTGCTGGGCTGCGTTGCGGTCAGCGCTGCGGGTGGCgtagt                     <  3:246656/149‑1 (MQ=255)
                                                                                                                                 cACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATTGTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGCCGTTGCTGGGCTGCGTTGCGGTCAGCGCTGCGGGTGGCgtagt                     <  5:287683/149‑1 (MQ=255)
                                                                                                                                  aCCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATTGTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGCCGTTGCTGGGCTGCGTTGCGGTCAGCGCTGCGGGTGGCgtagta                    <  7:92642/149‑1 (MQ=255)
                                                                                                                                  aCCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATTGTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGCCGTTGCTGGGCTGCGTTGCGGTCAGCGCTGCGGGTGGCgtagta                    <  3:215259/149‑1 (MQ=255)
                                                                                                                                      ggcggAACAAGAGCAATTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATTGTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGCCGTTGCTGGGCTGCGTTGCGGTCAGCGCTGCGGGTGGCGTAGTAGata                >  3:314927/1‑149 (MQ=255)
                                                                                                                                        cggAACAAGAGCAATTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATTGTCGGTACTGGCTATCCTTCTGCCGTTTGCTg                                                                         <  6:197416/90‑1 (MQ=39)
                                                                                                                                              aaTTGCTCTTGTTCCTCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATTGTCGGTACTGTCTATCCTTCTGCCGTTTGCTGGCCTGCAGCCGTTGCTGGGCTGCGTTGCGGTCAGCGCTGAGGGTGGCGTAGTAGATATCGGCCGt        <  1:177535/149‑1 (MQ=255)
                                                                                                                                              aaTTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATTGTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGCCGTTGCTGGGCTGCGTTGCGGTCAGCGCTGCGGGTGGCGTAGTAGATATCGGCCGt        <  1:171873/149‑1 (MQ=255)
                                                                                                                                                ttGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATTGTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGCCGTTGCTGGGCTGCGTTGCGGTCAGCGCTGCGGGTGGCGTAGTAGATATCGGCCGTgg      <  4:147122/149‑1 (MQ=255)
                                                                                                                                                  gCTCTTGTTCCGGCGCTTTGTTTGCTGGCATGGCAATCAATGACAATTGTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGCCGTTGCTGGGCTGCGTTGCGGTCAGCGCTGCGGGTGGCGTAGTAGATATCGGCCGTggg     <  7:155654/148‑1 (MQ=255)
                                                                                                                                                   ctctTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATTGTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGCCGTTGCTGGGCTGCGTTGCGGTCAGCGCTGCGGGTGGCGTAGTAGATATCGGCCGTGGGt    <  3:220834/148‑1 (MQ=255)
                                                                                                                                                   ctctTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATTGTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGCCGTTGCTGGGCTGCGTTGCGGTCAGCGCTGCGGGTGGCGTAGTAGATATCGGCCGTGGGTa   >  6:354480/1‑149 (MQ=255)
                                                                                                                                                    tctTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATTGTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGCCGt                                                             >  4:206109/1‑90 (MQ=255)
                                                                                                                                                    tctTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATTGTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGCCGTTGCTGGGCTGCGTTGCGGTCAGCGCTGCGGGTGGCGTAGTAGATATCGGCCGTg       >  7:3862/1‑144 (MQ=255)
                                                                                                                                                    tctTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATTGTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGCCGTTGCTGGGCTGCGTTGCGGTCAGCGCTGCGGGTGGCGTAGTAGATATCGGCCGTg       <  8:3862/144‑1 (MQ=255)
                                                                                                                                                    tctTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATTGTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGCCGTTGCTGGGCTGCGTTGCGGTCAGCGCTGCGGGTGGCGTAGTAGATATCGGCCGTGGGTAc  >  8:340018/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GTGCGTGAAGCAACCGCGCGTACCCGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATTGTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGCCGTTGCTGGGCTGCGTTGCGGTCAGCGCTGCGGGTGGCGTAGTAGATATCGGCCGTGGGTAC  >  NC_002947/494933‑495229

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑