Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,763,633	G→C	7.5%	A337P (GCC→CCC)	PP_5057 →	M23/M37 family peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,763,633	0	G	C	7.5%	208.7 / 7.2	67	A337P (GCC→CCC)	PP_5057	M23/M37 family peptidase
Reads supporting (aligned to +/- strand):  ref base G (29/33);  new base C (2/3);  total (31/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.96e-01

GCCCGTACCACCCTCGGGCCGATGGTTTCCAGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTACCTGAGCCTGTACGG  >  NC_002947/5763489‑5763781                                                                                                                                                  |                                                                                                                                                     gCCCGTACCACCCTCGGGCCGATGGTTTCCAGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCaa                                                                                                                                                  <  5:256214/149‑1 (MQ=255) gCCCGTACCACCCTCGGGCCGATGGTTTCCAGCGATGGCGCCAACTACGCCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGcc                                                                                                                                                    >  7:2245/1‑147 (MQ=255)         caccCTCGGGCCGATGGTTTCCAGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACg                                                                                                                                          >  3:14449/1‑149 (MQ=255)           cccTCGGGCCGATGGTTTCCAGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTCCCACTTGGCACggg                                                                                                                                        <  8:23924/149‑1 (MQ=255)            ccTCGGGCCGATGGTTTCCAGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACgggg                                                                                                                                       <  4:7247/149‑1 (MQ=255)              tCGGGCCGATGGTTTCCAGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGGAACAAAATTCCATGGCCAGGCAATGGTCGATTGGTGGCACGTTTCGGTGTTGCCCGGGGTTGCGACCCCCGGGCCAATTGGGACGGGGt                                                                                                                                      >  1:106721/1‑149 (MQ=255)               cggtactaTGTTTTCCAGCGATCGCTCAATCTTCCTCGGCGTATTTCCTCCTGCGCGGG‑GCAAACTTCCATTTCCTGCCAATGTTCTATTGTTGGTATGTTTCGGGGATTCCCGCGGTAGCGACGCTCGTGCCAAGTGTTACGTGGTTa                                                                                                                                    <  8:234844/142‑1 (MQ=255)               cGGGCCGATGGTTTCCAGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTa                                                                                                                                    >  3:23977/1‑149 (MQ=255)                    cGATGGTTTCCAGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGAt                                                                                                                                >  7:333101/1‑148 (MQ=255)                    cGATGGTTTCCAGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTCCCACTTGGCACgggcgtc                                                                                                                                    <  6:182882/144‑5 (MQ=255)                    cGATGGTTTCCAGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTCCCACTTGGCACgggcgtc                                                                                                                                    >  5:182882/1‑140 (MQ=255)                         gTTTCCAGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAgcgc                                                                                                                          >  5:234431/1‑149 (MQ=255)                          tttCCAGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGAAGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAgcgc                                                                                                                          >  2:100061/1‑148 (MQ=255)                              cAGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAgc                                                                                                                            >  2:248480/1‑142 (MQ=255)                              cAGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAgc                                                                                                                            <  1:248480/142‑1 (MQ=255)                               aGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAAccc                                                                                                                    <  5:238534/149‑1 (MQ=255)                                 cGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAg                                                                                                                  <  5:113304/149‑1 (MQ=255)                                   aTGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGc                                                                                                                <  4:23977/149‑1 (MQ=255)                                    tGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCa                                                                                                               <  6:234431/149‑1 (MQ=255)                                     ggCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCAc                                                                                                              >  3:114826/1‑149 (MQ=255)                                         ccAACTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAg                                                                                                          >  2:335740/1‑149 (MQ=255)                                          cAACTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACTGGGTTATGATCAGCGCCAACCCAGGCACCCAgg                                                                                                         <  4:221514/149‑1 (MQ=255)                                          cAACTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAgg                                                                                                         >  8:174786/1‑149 (MQ=255)                                          cAACTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTCCCACTTGGCACgg                                                                                                                                         >  4:99589/1‑117 (MQ=255)                                          cAACTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTCCCACTTGGCACgg                                                                                                                                         <  3:99589/117‑1 (MQ=255)                                            aCTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCAAAGTGGGACGGGGTTATGATCAGCGCAAACCCAGGCACCCAGGTa                                                                                                       >  4:115852/1‑149 (MQ=255)                                             cTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTAc                                                                                                      <  7:174786/149‑1 (MQ=255)                                                 ggcggcGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTAcgtgc                                                                                                  >  4:278122/1‑149 (MQ=255)                                                       gcATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCa                                                                                            <  4:14449/149‑1 (MQ=255)                                                          ttttCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCAcgg                                                                                         >  8:181949/1‑149 (MQ=255)                                                            ttCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCAcggc                                                                                        <  6:184851/148‑1 (MQ=255)                                                                    cgcgGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCgtgt                                                                                <  4:114826/148‑1 (MQ=255)                                                                    cgcgGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGtgtg                                                                               <  1:335740/149‑1 (MQ=255)                                                                    cgcgGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGtgtg                                                                               <  2:106721/149‑1 (MQ=255)                                                                        gg‑gCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCgtgt                                                                                <  4:178571/144‑1 (MQ=255)                                                                        gg‑gCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCgtgt                                                                                >  3:178571/1‑144 (MQ=255)                                                                              aaCTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTcgcg                                                                      >  4:83857/1‑149 (MQ=255)                                                                                 ttCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGAc                                                                   >  8:257264/1‑149 (MQ=255)                                                                                 ttCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGAc                                                                   >  1:32143/1‑149 (MQ=255)                                                                                   ccATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCAACCAGCTACGTGCCCTGAACGGCGGGCGTGTGGTATTACCGGACTg                                                                 >  8:327931/1‑149 (MQ=255)                                                                                    cttGGCCATTCAATGGTCGTTTGCTGTCACGTTTCGGTGATGCCCGCGGTAGCGAGGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGGCGTGAACGGCGGGCGTGTTGTATTGGCGGACTg                                                                 <  6:300352/146‑1 (MQ=255)                                                                                     aTGGCCAGTAAATGGTCGATTGCTGGCACGTTTCCCTGAAGCCCGCGGTAGCGACGCCCCTGCCAAGTGGGACGTGGTTATGATCAGCGACAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGt                                                               >  2:351475/1‑149 (MQ=255)                                                                                      tGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGtt                                                              <  8:333101/149‑1 (MQ=255)                                                                                             tCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGt                                                       <  1:134251/149‑1 (MQ=255)                                                                                             tCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGt                                                       >  5:333074/1‑149 (MQ=255)                                                                                             tCAATGCTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGAGGGGTATTCGCGGAGTGGTTGCGCGGt                                                       >  8:337261/1‑149 (MQ=255)                                                                                               aaTGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGc                                                     >  1:241313/1‑149 (MQ=255)                                                                                               aaTGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGc                                                     >  6:77122/1‑149 (MQ=255)                                                                                                   gTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCggg                                                 <  6:333074/149‑1 (MQ=255)                                                                                                    tCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGc                                                <  3:278122/149‑1 (MQ=255)                                                                                                       aTTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTc                                             >  3:336479/1‑149 (MQ=255)                                                                                                              gCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCAt                                      >  3:133759/1‑149 (MQ=255)                                                                                                                     tCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGAAGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGa                                <  3:115852/148‑1 (MQ=255)                                                                                                                      cGGTGATGCCCGCGGTAGCGTCGCCCGTGCCAAGTGGGACGGGGTTATGATAAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGAc                               <  7:327931/148‑1 (MQ=255)                                                                                                                       ggTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATg                                                                                                                                  <  1:57992/48‑1 (MQ=255)                                                                                                                       ggTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATg                                                                                                                                  >  2:57992/1‑48 (MQ=255)                                                                                                                               ccGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCACCCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGCCCATGGCAACg                     >  8:250867/1‑149 (MQ=255)                                                                                                                               ccGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACg                     <  2:32143/149‑1 (MQ=255)                                                                                                                                   ggTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTa                 <  6:265687/149‑1 (MQ=255)                                                                                                                                    gTAGCGACGCCGGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCTGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGTGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTAc                <  1:100061/149‑1 (MQ=255)                                                                                                                                    gTAGCGACGCCCGTGCCAAGTGGGTCGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTAc                <  5:44052/149‑1 (MQ=255)                                                                                                                                    gTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTAc                >  2:365882/1‑149 (MQ=255)                                                                                                                                       gCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTACCt              <  8:2245/148‑1 (MQ=255)                                                                                                                                          aCGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTACCTGAGc          <  7:181949/149‑1 (MQ=255)                                                                                                                                          aCGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTACCTGAGc          <  4:336479/149‑1 (MQ=255)                                                                                                                                                gTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTACCTGAGCCTGTAc    <  1:365882/149‑1 (MQ=255)                                                                                                                                                 tGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTACCTGAGCCTGTACg   <  3:83857/149‑1 (MQ=255)                                                                                                                                                  gCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTACCTGAGCCTGTACgg  >  7:229005/1‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                                     GCCCGTACCACCCTCGGGCCGATGGTTTCCAGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTACCTGAGCCTGTACGG  >  NC_002947/5763489‑5763781

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑