Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,763,638	G→T	8.5%	K338N (AAG→AAT) ‡	PP_5057 →	M23/M37 family peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,763,638	0	G	T	8.5%	216.7 / 7.2	69	K338N (AAG→AAT) ‡	PP_5057	M23/M37 family peptidase
Reads supporting (aligned to +/- strand):  ref base G (30/33);  new base T (3/3);  total (33/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.72e-01

CACCCTCGGGCCGATGGTTTCCAGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTACCTGAGCCTGTACGGCCA  >  NC_002947/5763497‑5763784                                                                                                                                               |                                                                                                                                                   caccCTCGGGCCGATGGTTTCCAGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACg                                                                                                                                             >  3:14449/1‑149 (MQ=255)   cccTCGGGCCGATGGTTTCCAGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTCCCACTTGGCACggg                                                                                                                                           <  8:23924/149‑1 (MQ=255)    ccTCGGGCCGATGGTTTCCAGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACgggg                                                                                                                                          <  4:7247/149‑1 (MQ=255)      tCGGGCCGATGGTTTCCAGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGGAACAAAATTCCATGGCCAGGCAATGGTCGATTGGTGGCACGTTTCGGTGTTGCCCGGGGTTGCGACCCCCGGGCCAATTGGGACGGGGt                                                                                                                                         >  1:106721/1‑149 (MQ=255)       cggtactaTGTTTTCCAGCGATCGCTCAATCTTCCTCGGCGTATTTCCTCCTGCGCGGG‑GCAAACTTCCATTTCCTGCCAATGTTCTATTGTTGGTATGTTTCGGGGATTCCCGCGGTAGCGACGCTCGTGCCAAGTGTTACGTGGTTa                                                                                                                                       <  8:234844/142‑1 (MQ=255)       cGGGCCGATGGTTTCCAGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTa                                                                                                                                       >  3:23977/1‑149 (MQ=255)            cGATGGTTTCCAGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGAt                                                                                                                                   >  7:333101/1‑148 (MQ=255)            cGATGGTTTCCAGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTCCCACTTGGCACgggcgtc                                                                                                                                       >  5:182882/1‑140 (MQ=255)            cGATGGTTTCCAGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTCCCACTTGGCACgggcgtc                                                                                                                                       <  6:182882/144‑5 (MQ=255)                 gTTTCCAGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAgcgc                                                                                                                             >  5:234431/1‑149 (MQ=255)                  tttCCAGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGAAGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAgcgc                                                                                                                             >  2:100061/1‑148 (MQ=255)                      cAGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAgc                                                                                                                               >  2:248480/1‑142 (MQ=255)                      cAGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAgc                                                                                                                               <  1:248480/142‑1 (MQ=255)                       aGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAAccc                                                                                                                       <  5:238534/149‑1 (MQ=255)                         cGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAg                                                                                                                     <  5:113304/149‑1 (MQ=255)                           aTGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGc                                                                                                                   <  4:23977/149‑1 (MQ=255)                            tGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCa                                                                                                                  <  6:234431/149‑1 (MQ=255)                             ggCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCAc                                                                                                                 >  3:114826/1‑149 (MQ=255)                                 ccAACTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAg                                                                                                             >  2:335740/1‑149 (MQ=255)                                  cAACTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACTGGGTTATGATCAGCGCCAACCCAGGCACCCAgg                                                                                                            <  4:221514/149‑1 (MQ=255)                                  cAACTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAgg                                                                                                            >  8:174786/1‑149 (MQ=255)                                  cAACTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTCCCACTTGGCACgg                                                                                                                                            >  4:99589/1‑117 (MQ=255)                                  cAACTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTCCCACTTGGCACgg                                                                                                                                            <  3:99589/117‑1 (MQ=255)                                    aCTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCAAAGTGGGACGGGGTTATGATCAGCGCAAACCCAGGCACCCAGGTa                                                                                                          >  4:115852/1‑149 (MQ=255)                                     cTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTAc                                                                                                         <  7:174786/149‑1 (MQ=255)                                         ggcggcGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTAcgtgc                                                                                                     >  4:278122/1‑149 (MQ=255)                                               gcATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCa                                                                                               <  4:14449/149‑1 (MQ=255)                                                  ttttCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCAcgg                                                                                            >  8:181949/1‑149 (MQ=255)                                                    ttCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCAcggc                                                                                           <  6:184851/148‑1 (MQ=255)                                                            cgcgGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCgtgt                                                                                   <  4:114826/148‑1 (MQ=255)                                                            cgcgGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGtgtg                                                                                  <  2:106721/149‑1 (MQ=255)                                                            cgcgGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGtgtg                                                                                  <  1:335740/149‑1 (MQ=255)                                                                gg‑gCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCgtgt                                                                                   <  4:178571/144‑1 (MQ=255)                                                                gg‑gCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCgtgt                                                                                   >  3:178571/1‑144 (MQ=255)                                                                      aaCTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTcgcg                                                                         >  4:83857/1‑149 (MQ=255)                                                                         ttCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGAc                                                                      >  8:257264/1‑149 (MQ=255)                                                                         ttCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGAc                                                                      >  1:32143/1‑149 (MQ=255)                                                                           ccATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCAACCAGCTACGTGCCCTGAACGGCGGGCGTGTGGTATTACCGGACTg                                                                    >  8:327931/1‑149 (MQ=255)                                                                            cttGGCCATTCAATGGTCGTTTGCTGTCACGTTTCGGTGATGCCCGCGGTAGCGAGGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGGCGTGAACGGCGGGCGTGTTGTATTGGCGGACTg                                                                    <  6:300352/146‑1 (MQ=255)                                                                             aTGGCCAGTAAATGGTCGATTGCTGGCACGTTTCCCTGAAGCCCGCGGTAGCGACGCCCCTGCCAAGTGGGACGTGGTTATGATCAGCGACAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGt                                                                  >  2:351475/1‑149 (MQ=255)                                                                              tGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGtt                                                                 <  8:333101/149‑1 (MQ=255)                                                                                     tCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGt                                                          <  1:134251/149‑1 (MQ=255)                                                                                     tCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGt                                                          >  5:333074/1‑149 (MQ=255)                                                                                     tCAATGCTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGAGGGGTATTCGCGGAGTGGTTGCGCGGt                                                          >  8:337261/1‑149 (MQ=255)                                                                                       aaTGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGc                                                        >  1:241313/1‑149 (MQ=255)                                                                                       aaTGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGc                                                        >  6:77122/1‑149 (MQ=255)                                                                                           gTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCggg                                                    <  6:333074/149‑1 (MQ=255)                                                                                            tCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGc                                                   <  3:278122/149‑1 (MQ=255)                                                                                               aTTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTc                                                >  3:336479/1‑149 (MQ=255)                                                                                                      gCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCAt                                         >  3:133759/1‑149 (MQ=255)                                                                                                             tCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGAAGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGa                                   <  3:115852/148‑1 (MQ=255)                                                                                                              cGGTGATGCCCGCGGTAGCGTCGCCCGTGCCAAGTGGGACGGGGTTATGATAAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGAc                                  <  7:327931/148‑1 (MQ=255)                                                                                                               ggTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATg                                                                                                                                     <  1:57992/48‑1 (MQ=255)                                                                                                               ggTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATg                                                                                                                                     >  2:57992/1‑48 (MQ=255)                                                                                                                       ccGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCACCCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGCCCATGGCAACg                        >  8:250867/1‑149 (MQ=255)                                                                                                                       ccGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACg                        <  2:32143/149‑1 (MQ=255)                                                                                                                           ggTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTa                    <  6:265687/149‑1 (MQ=255)                                                                                                                            gTAGCGACGCCGGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCTGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGTGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTAc                   <  1:100061/149‑1 (MQ=255)                                                                                                                            gTAGCGACGCCCGTGCCAAGTGGGTCGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTAc                   <  5:44052/149‑1 (MQ=255)                                                                                                                            gTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTAc                   >  2:365882/1‑149 (MQ=255)                                                                                                                               gCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTACCt                 <  8:2245/148‑1 (MQ=255)                                                                                                                                  aCGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTACCTGAGc             <  7:181949/149‑1 (MQ=255)                                                                                                                                  aCGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTACCTGAGc             <  4:336479/149‑1 (MQ=255)                                                                                                                                        gTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTACCTGAGCCTGTAc       <  1:365882/149‑1 (MQ=255)                                                                                                                                         tGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTACCTGAGCCTGTACg      <  3:83857/149‑1 (MQ=255)                                                                                                                                          gCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTACCTGAGCCTGTACgg     >  7:229005/1‑149 (MQ=255)                                                                                                                                           ccAAGTGTGACGGGGTTATGTTCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTACCTGAGCCTGTACGGc    <  7:337261/149‑1 (MQ=255)                                                                                                                                            cAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTACCTGAGCCTGTACGGc    >  7:208650/1‑148 (MQ=255)                                                                                                                                             aaGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGTGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTACCTGAGCCTGTACGGCca  >  2:229667/1‑149 (MQ=255)                                                                                                                                               |                                                                                                                                                   CACCCTCGGGCCGATGGTTTCCAGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTACCTGAGCCTGTACGGCCA  >  NC_002947/5763497‑5763784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑