Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,763,642 G→C 7.3% D340H (GAC→CAC)  PP_5057 → M23/M37 family peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,763,6420GC 7.3% 214.4 / 7.1 69D340H (GAC→CAC) PP_5057M23/M37 family peptidase
Reads supporting (aligned to +/- strand):  ref base G (31/32);  new base C (2/3);  total (33/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.82e-01

CACCCTCGGGCCGATGGTTTCCAGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTACCTGAGCCTGTACGGCCACAACCA  >  NC_002947/5763497‑5763790
                                                                                                                                                  |                                                                                                                                                    
caccCTCGGGCCGATGGTTTCCAGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACg                                                                                                                                                   >  3:14449/1‑149 (MQ=255)
  cccTCGGGCCGATGGTTTCCAGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTCCCACTTGGCACggg                                                                                                                                                 <  8:23924/149‑1 (MQ=255)
   ccTCGGGCCGATGGTTTCCAGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACgggg                                                                                                                                                <  4:7247/149‑1 (MQ=255)
     tCGGGCCGATGGTTTCCAGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGGAACAAAATTCCATGGCCAGGCAATGGTCGATTGGTGGCACGTTTCGGTGTTGCCCGGGGTTGCGACCCCCGGGCCAATTGGGACGGGGt                                                                                                                                               >  1:106721/1‑149 (MQ=255)
      cggtactaTGTTTTCCAGCGATCGCTCAATCTTCCTCGGCGTATTTCCTCCTGCGCGGG‑GCAAACTTCCATTTCCTGCCAATGTTCTATTGTTGGTATGTTTCGGGGATTCCCGCGGTAGCGACGCTCGTGCCAAGTGTTACGTGGTTa                                                                                                                                             <  8:234844/142‑1 (MQ=255)
      cGGGCCGATGGTTTCCAGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTa                                                                                                                                             >  3:23977/1‑149 (MQ=255)
           cGATGGTTTCCAGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGAt                                                                                                                                         >  7:333101/1‑148 (MQ=255)
           cGATGGTTTCCAGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTCCCACTTGGCACgggcgtc                                                                                                                                             <  6:182882/144‑5 (MQ=255)
           cGATGGTTTCCAGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTCCCACTTGGCACgggcgtc                                                                                                                                             >  5:182882/1‑140 (MQ=255)
                gTTTCCAGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAgcgc                                                                                                                                   >  5:234431/1‑149 (MQ=255)
                 tttCCAGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGAAGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAgcgc                                                                                                                                   >  2:100061/1‑148 (MQ=255)
                     cAGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAgc                                                                                                                                     <  1:248480/142‑1 (MQ=255)
                     cAGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAgc                                                                                                                                     >  2:248480/1‑142 (MQ=255)
                      aGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAAccc                                                                                                                             <  5:238534/149‑1 (MQ=255)
                        cGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAg                                                                                                                           <  5:113304/149‑1 (MQ=255)
                          aTGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGc                                                                                                                         <  4:23977/149‑1 (MQ=255)
                           tGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCa                                                                                                                        <  6:234431/149‑1 (MQ=255)
                            ggCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCAc                                                                                                                       >  3:114826/1‑149 (MQ=255)
                                ccAACTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAg                                                                                                                   >  2:335740/1‑149 (MQ=255)
                                 cAACTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACTGGGTTATGATCAGCGCCAACCCAGGCACCCAgg                                                                                                                  <  4:221514/149‑1 (MQ=255)
                                 cAACTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAgg                                                                                                                  >  8:174786/1‑149 (MQ=255)
                                 cAACTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTCCCACTTGGCACgg                                                                                                                                                  >  4:99589/1‑117 (MQ=255)
                                 cAACTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTCCCACTTGGCACgg                                                                                                                                                  <  3:99589/117‑1 (MQ=255)
                                   aCTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCAAAGTGGGACGGGGTTATGATCAGCGCAAACCCAGGCACCCAGGTa                                                                                                                >  4:115852/1‑149 (MQ=255)
                                    cTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTAc                                                                                                               <  7:174786/149‑1 (MQ=255)
                                        ggcggcGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTAcgtgc                                                                                                           >  4:278122/1‑149 (MQ=255)
                                              gcATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCa                                                                                                     <  4:14449/149‑1 (MQ=255)
                                                 ttttCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCAcgg                                                                                                  >  8:181949/1‑149 (MQ=255)
                                                   ttCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCAcggc                                                                                                 <  6:184851/148‑1 (MQ=255)
                                                           cgcgGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCgtgt                                                                                         <  4:114826/148‑1 (MQ=255)
                                                           cgcgGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGtgtg                                                                                        <  2:106721/149‑1 (MQ=255)
                                                           cgcgGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGtgtg                                                                                        <  1:335740/149‑1 (MQ=255)
                                                               gg‑gCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCgtgt                                                                                         <  4:178571/144‑1 (MQ=255)
                                                               gg‑gCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCgtgt                                                                                         >  3:178571/1‑144 (MQ=255)
                                                                     aaCTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTcgcg                                                                               >  4:83857/1‑149 (MQ=255)
                                                                        ttCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGAc                                                                            >  8:257264/1‑149 (MQ=255)
                                                                        ttCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGAc                                                                            >  1:32143/1‑149 (MQ=255)
                                                                          ccATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCAACCAGCTACGTGCCCTGAACGGCGGGCGTGTGGTATTACCGGACTg                                                                          >  8:327931/1‑149 (MQ=255)
                                                                           cttGGCCATTCAATGGTCGTTTGCTGTCACGTTTCGGTGATGCCCGCGGTAGCGAGGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGGCGTGAACGGCGGGCGTGTTGTATTGGCGGACTg                                                                          <  6:300352/146‑1 (MQ=255)
                                                                            aTGGCCAGTAAATGGTCGATTGCTGGCACGTTTCCCTGAAGCCCGCGGTAGCGACGCCCCTGCCAAGTGGGACGTGGTTATGATCAGCGACAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGt                                                                        >  2:351475/1‑149 (MQ=255)
                                                                             tGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGtt                                                                       <  8:333101/149‑1 (MQ=255)
                                                                                    tCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGt                                                                <  1:134251/149‑1 (MQ=255)
                                                                                    tCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGt                                                                >  5:333074/1‑149 (MQ=255)
                                                                                    tCAATGCTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGAGGGGTATTCGCGGAGTGGTTGCGCGGt                                                                >  8:337261/1‑149 (MQ=255)
                                                                                      aaTGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGc                                                              >  1:241313/1‑149 (MQ=255)
                                                                                      aaTGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGc                                                              >  6:77122/1‑149 (MQ=255)
                                                                                          gTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCggg                                                          <  6:333074/149‑1 (MQ=255)
                                                                                           tCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGc                                                         <  3:278122/149‑1 (MQ=255)
                                                                                              aTTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTc                                                      >  3:336479/1‑149 (MQ=255)
                                                                                                     gCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCAt                                               >  3:133759/1‑149 (MQ=255)
                                                                                                            tCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGAAGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGa                                         <  3:115852/148‑1 (MQ=255)
                                                                                                             cGGTGATGCCCGCGGTAGCGTCGCCCGTGCCAAGTGGGACGGGGTTATGATAAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGAc                                        <  7:327931/148‑1 (MQ=255)
                                                                                                              ggTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATg                                                                                                                                           <  1:57992/48‑1 (MQ=255)
                                                                                                              ggTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATg                                                                                                                                           >  2:57992/1‑48 (MQ=255)
                                                                                                                      ccGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCACCCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGCCCATGGCAACg                              >  8:250867/1‑149 (MQ=255)
                                                                                                                      ccGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACg                              <  2:32143/149‑1 (MQ=255)
                                                                                                                          ggTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTa                          <  6:265687/149‑1 (MQ=255)
                                                                                                                           gTAGCGACGCCGGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCTGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGTGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTAc                         <  1:100061/149‑1 (MQ=255)
                                                                                                                           gTAGCGACGCCCGTGCCAAGTGGGTCGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTAc                         <  5:44052/149‑1 (MQ=255)
                                                                                                                           gTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTAc                         >  2:365882/1‑149 (MQ=255)
                                                                                                                              gCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTACCt                       <  8:2245/148‑1 (MQ=255)
                                                                                                                                 aCGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTACCTGAGc                   <  7:181949/149‑1 (MQ=255)
                                                                                                                                 aCGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTACCTGAGc                   <  4:336479/149‑1 (MQ=255)
                                                                                                                                       gTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTACCTGAGCCTGTAc             <  1:365882/149‑1 (MQ=255)
                                                                                                                                        tGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTACCTGAGCCTGTACg            <  3:83857/149‑1 (MQ=255)
                                                                                                                                         gCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTACCTGAGCCTGTACgg           >  7:229005/1‑149 (MQ=255)
                                                                                                                                          ccAAGTGTGACGGGGTTATGTTCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTACCTGAGCCTGTACGGc          <  7:337261/149‑1 (MQ=255)
                                                                                                                                           cAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTACCTGAGCCTGTACGGc          >  7:208650/1‑148 (MQ=255)
                                                                                                                                            aaGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGTGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTACCTGAGCCTGTACGGCca        >  2:229667/1‑149 (MQ=255)
                                                                                                                                                gggACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTc                                                      >  8:14316/1‑99 (MQ=255)
                                                                                                                                                 ggACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTACCTGAGCCTGTACGGCCACAAc    >  1:143267/1‑148 (MQ=255)
                                                                                                                                                  gACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTACCTGAGCCTGTACGGCCACAACCa  >  1:138907/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
CACCCTCGGGCCGATGGTTTCCAGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGG‑GCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTACCTGAGCCTGTACGGCCACAACCA  >  NC_002947/5763497‑5763790

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑