Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	495,716	A→G	5.7%	N106N (AAT→AAC)	PP_0408 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	495,716	0	A	G	5.7%	210.3 / 2.1	70	N106N (AAT→AAC)	PP_0408	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (35/31);  new base G (2/2);  total (37/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

TCGCCTTTGTCTGGCGCAGGCGCTGCCGGTGGTGTGGCTTCGACCCGCGCTTGCGGGCTGTCTGCGAGCAGGTCGGGCAGGCTGACGCTCAGGCTGTGCCAGCCGATATCAGGGAATTTTTGCCGCAGTGGGCCGACTGCATTTGGCCA‑GTCGGCCGTTTCGCCTGCACCAGGGACAATGATCACCGCCCCTTGCGGATCGTTGTCGTTGGCCGGCTTCCACAGGGCCAGGAAGCTGTCGGCACCGGCCTGCAGGGTCTGCTGCTCAGCCTTGGGCAGCAGCCGT  >  NC_002947/495576‑495860                                                                                                                                             |                                                                                                                                                  tCGCCTTTGTCTGGCGCAGGCGCTGCCGGTGGTGTGGCTTCGACCCGCGCTTGCGGGCTGTCTGCGATCAGGTCGGGCAGGCTGACGCTCAGGCTGTGCCAGCCGATATCAGGGAATTTTTGCCGCAGTGGGCCGACTGCATTTGGc                                                                                                                                             <  5:173512/147‑1 (MQ=255)   gCCTTTGTCTGGCGCAGGCGCTGCCGGTGGTGTGGCTTCGACCCGCGCTTGCGGGCTGTCTGCGAGCAGGTCGGGCAGGCTGACGCTCAGGCTGTGCCAGCCGATATCAGGGAATTTTTGCCGCAGTGGGCCGACTGCATTTGGCCA‑Gt                                                                                                                                        >  5:89976/1‑149 (MQ=255)          tCTGGCGCAGGCGCTGCCGGTGGTGTGGCTTCGACCCGCGCTTGCGGGCTGTCTGCGAGCAGGTCGGGCAGGCTGACGCTCAGGCTGTGCCAGCCGATATCAGGGAATTTTTGCCGCAGTGGGCCGACTGCATTTGGc                                                                                                                                             <  6:314722/138‑1 (MQ=255)          tCTGGCGCAGGCGCTGCCGGTGGTGTGGCTTCGACCCGCGCTTGCGGGCTGTCTGCGAGCAGGTCGGGCAGGCTGACGCTCAGGCTGTGCCAGCCGATATCAGGGAATTTTTGCCGCAGTGGGCCGACTGCATTTGGc                                                                                                                                             >  5:314722/1‑138 (MQ=255)            tGGCGCAGGCGCTGCCGGTGGTGTGGCTTCGACCCGCGCTTGCGGGCTGTCTGCGAGCAGGTCGGGCAGGCTGACGCTCAGGCTGTGCCAGCCGATATCAGGGAATTTTTGCCGCAGTGGGCCGACTGCATTTGGCCa                                                                                                                                           >  8:326634/1‑138 (MQ=255)            tGGCGCAGGCGCTGCCGGTGGTGTGGCTTCGACCCGCGCTTGCGGGCTGTCTGCGAGCAGGTCGGGCAGGCTGACGCTCAGGCTGTGCCAGCCGATATCAGGGAATTTTTGCCGCAGTGGGCCGACTGCATTTGGCCa                                                                                                                                           <  7:326634/138‑1 (MQ=255)             ggCGCAGGCGCTGCCGGTGGTGTGGCTTCGACCCGCGCTTGCGGGCTGTCTGCGAGCAGGTCGGGCAGGCTGACGCTCAGGCTGTGCCAGCCGATATCAGGGAATTTTTGCCGCAGTGGGCCGACTGCATTTGGCCA‑GTCGGCCGTTTc                                                                                                                              <  8:176352/149‑1 (MQ=255)                             gtggtgTGGCTTCGACCCGCGCTTGCGGGCTGTCTGCGAGCAGGTCGGGCAGGCTGACGCTCAGGCTGTGCCAGCCGATATCAGGGAATTTTTGCCGCAGTGGGCCGACTGCATTTgg                                                                                                                                              >  6:147629/1‑118 (MQ=255)                                gtgtGGCTTCGACCCGCGCTTGCGGGCTGTCTGCGAGCAGGTCGGGCAGGCTGACGCTCAGGCTGTGCCAGCCGATATCAGGGAATTTTTGCCGCAGTGGGCCGACTGCATTTGGCCA‑GTCGGCCGTTTCGCCTGCACCAGGGACAAt                                                                                                            >  6:343916/1‑148 (MQ=255)                                   tgGGTTCGAGCCGCGCTTGCGGGCTGTCTGCGAGCAGGTCGGGCAGGCTGACGCTCAGGCTGTGCCAGCCGATATCAGGGAATTTTTGCCGCAGTGGGCCGACTGCATTTGGCCA‑GTCGGCCGTTTCGCCTGCACCAGGGACAATTAt                                                                                                         <  6:89976/148‑1 (MQ=255)                                     gCTTCGACCCGCGCTTGCGGGCTGTCTGCGAGCAGGTCGGGCAGGCTGACGCTCAGGCTGTGCCAGCCGATATCAGGGAATTTTTGCCGCAGTGGGCCGACTGCATTTGGCCA‑GTCGGCCGtt                                                                                                                                >  3:94422/1‑123 (MQ=255)                                     gCTTCGACCCGCGCTTGCGGGCTGTCTGCGAGCAGGTCGGGCAGGCTGACGCTCAGGCTGTGCCAGCCGATATCAGGGAATTTTTGCCGCAGTGGGCCGACTGCATTTGGCCA‑GTCGGCCGtt                                                                                                                                <  4:94422/123‑1 (MQ=255)                                     gCTTCGACCCGCGCTTGCGGGCTGTCTGCGAGCAGGTCGGGCAGGCTGACGCTCAGGCTGTGCCAGCCGATATCAGGGAATTTTTGCCGCAGTGGGCCGACTGCATTTGGCCA‑GTCGGCCGTTTCGCCTGCACCAGGGACAATGAt                                                                                                         >  8:238096/1‑146 (MQ=255)                                     gCTTCGACCCGCGCTTGCGGGCTGTCTGCGAGCAGGTCGGGCAGGCTGACGCTCAGGCTGTGCCAGCCGATATCAGGGAATTTTTGCCGCAGTGGGCCGACTGCATTTGGCCA‑GTCGGCCGTTTCGCCTGCACCAGGGACAATGAt                                                                                                         <  7:238096/146‑1 (MQ=255)                                     gCTTCGACCCGCGCTTGCGGGCTGTCTGCGAGCAGGTCGGGCAGGCTGACGCTCAGGCTGTGCCAGCCGATATCAGGGAATTTTTGCCGCAGTGGGCCGACTGCATTTGGCCA‑GTCGGCCGTTTCGCCTGCACCAGGGACAATGATCAc                                                                                                      >  2:136347/1‑149 (MQ=255)                                     gCTTCGACCCGCGCTTGCGGGCTGTCTGCGAGCAGGTCGGGCAGGCTGACGCTCAGGCTGTGCCAGCCGATATCAGGGAATTTTTGCCGCAGTGGGCCGACTGCATTTGGCCA‑GTCGGCCGTTTCGCCTGCACCAGGGACAATGATCAc                                                                                                      <  1:328376/149‑1 (MQ=255)                                       ttCGACCCGCGCTTGCGGGCTGTCTGCGAGCAGGTCGGGCAGGCTGACGCTCAGGCTGTGCCAGCCGATATCAGGGAATTTTTGCCGCAGTGGGCCGACTGCGTTTGGCCA‑GTCGGCCGTTTCGCCTGCACCAGGGACAATGATCACCg                                                                                                    >  3:50028/1‑149 (MQ=255)                                       ttCGACCCGCGCTTGCGGGCTGTCTGCGAGCAGGTCGGGCAGGCTGACGCTCAGGCTATGCCAGCCGATATCAGGGAATTTTTGCCGCAGTGGGCCGACTGCATTTGGCCA‑GTCGGCCGTTTCGCCTGCACCAGGGACAAt                                                                                                            <  7:326256/141‑1 (MQ=255)                                       ttCGACCCGCGCTTGCGGGCTGTCTGCGAGCAGGTCGGGCAGGCTGACGCTCAGGCTATGCCAGCCGATATCAGGGAATTTTTGCCGCAGTGGGCCGACTGCATTTGGCCA‑GTCGGCCGTTTCGCCTGCACCAGGGACAAt                                                                                                            >  8:326256/1‑141 (MQ=255)                                             ccGCGCTTGCGGGCTGTCTGCGAGCAGGTCGGGCAGGCTGACGCTCAGGCTGTGCCAGCCGATATCAGGGAATTTTTGCCGCAGTGGGCCGACTGCATTTGGCCA‑GTCGGCCGTTTCGCCTGCACCAGGGACAATGATCACCGCCCCtt                                                                                              >  2:331296/1‑149 (MQ=255)                                              cgcgCTTGCGGGCTGTCTGCGAGCAGGTCGGGCAGGCTGACGCTCAGGCTGTGCCAGCCGATATCAGGGAATTTTTGCCGCAGTGGGCCGACTGCATTTGGCCA‑GTCGGCCGTTTCGCCTGCACCAGGGACAATGATCACCGCCCCTTg                                                                                             >  1:331602/1‑149 (MQ=255)                                               gggcTTGCGGGCTGTCTGCGAGCAGGTCTGGCAGGCTGACGCTCAGGCTGTGCCAGCCGATATCAGGTAATTTTTGCCGCAGTGGGCCGACTGCATTTGGCCA‑GTCGGCCGTTTCGCCTGCACCAGGGACAATGATCACCGCCCCTTg                                                                                             <  8:34876/146‑1 (MQ=255)                                               gcgcTTGCGGGCTGTCTGCGAGCAGGTCGGGCAGGCTGACGCTCAGGCTGTGCCAGCCGATATCAGGGAATTTTTGCCGCAGTGGGCCGACTGCGTTTGGCCA‑GTCGGCCGTTTCGCCTGCACCAGGGACAATGATCACCGCCCCTTGc                                                                                            <  4:50028/149‑1 (MQ=255)                                               gcgcTTGCGGGCTGTCTGCGAGCAGGTCGGGCAGGCTGACGCTCAGGCTGTGCCAGCCGATATCAGGGAATTTTTGCCGCAGTGGGCCGACTGCATTTGGCCA‑GTCGGCCGTTTCGCCTGCACCAGGGACAATGATCACCGCCCCTTGc                                                                                            >  7:34876/1‑149 (MQ=255)                                                       gggCTGTCTGCGAGCAGGTCGGGCAGGCTGACGCTCAGGCTGTGCCAGCCGATATCAGGGAATTTTTGCCGCAGTGGGCCGACTGCGTTTGGCCA‑GTCGGCCGTTTCGCCTGCACCAGGGACAATGATCACCGCCCCTTGCGGAtcgtt                                                                                    >  7:164680/1‑149 (MQ=255)                                                       gggCTGTCTGCGAGCAGGTCGGGCAGGCTGACGCTCAGGCTGTGCCAGCCGATATCAGGGAATTTTTGCCGCAGTGGGCCGACTGCATTTGGCCA‑GTCGGCCGTTTCGCCTGCACCAGGGACAATGATCACCGCCCCTTGCGGAtcgtt                                                                                    >  2:42672/1‑149 (MQ=255)                                                           tGTCTGCGAGCAGGTCGGGCAGGCTGACGCTCAGGCTGTGCCAGCCGATATCAGGGAATTTTTGCCGCAGTGGGCCGACTGCATTTGGCCA‑GTCGGCCGTTTCGCCTGCACCAGGGACAATGATCACCGCCCCTTGCGGAtcgttgtcg                                                                                >  8:190191/1‑149 (MQ=255)                                                               tGCGAGCAGGTCGGGCAGGCTGACGCTCAGGCTGTGCCAGCCGATATCAGGGAATTTTTGCGGCAGTGGGCCGACTGCATTTGGCCA‑GTCGGCCGTTTCGCCTGCACCAGGGACAATGATCACCGCCCCTTGCGGATCGTTGTCGTTgg                                                                            >  6:190796/1‑149 (MQ=255)                                                               tGCGAGCAGGTCGGGCAGGCTGACGCTCAGGCTGTGCCAGCCGATATCAGGGAATTTTTGCCGCAGTGGGCCGACTGCATTTGGCCA‑GTCGGCCGTTTCGCCTGCACCAGGGACAATGATCACCGCCCCTTGCGGAtcgtt                                                                                    >  3:23866/1‑141 (MQ=255)                                                               tGCGAGCAGGTCGGGCAGGCTGACGCTCAGGCTGTGCCAGCCGATATCAGGGAATTTTTGCCGCAGTGGGCCGACTGCATTTGGCCA‑GTCGGCCGTTTCGCCTGCACCAGGGACAATGATCACCGCCCCTTGCGGAtcgtt                                                                                    <  4:23866/141‑1 (MQ=255)                                                                 cGAGCAGGTCGGGCAGGCTGACGCTCAGGCTGTGCCAGCCGATATCAGGGAATTTTTGCCGCAGTGGGCCGACTGCATTTGGCCA‑GTCGGCCGTTTCGCCTGCACCAGGGACAATGATCACCGCCCCTTGCGGAtcgttgtcgttg                                                                             <  1:136347/146‑1 (MQ=255)                                                                 cGAGCAGGTCGGGCAGGCTGACGCTCAGGCTGTGCCAGCCGATATCAGGGAATTTTTGCCGCAGTGGGCCGACTGCATTTGGCCA‑GTCGGCCGTTTCGCCTGCACCAGGGACAATGATCACCGCCCCTTGCGGATCGTTGTCGTTgg                                                                            <  1:331296/147‑1 (MQ=255)                                                                 cGAGCAGGTCGGGCAGGCTGACGCTCAGGCTGTGCCAGCCGATATCAGGGAATTTTTGCCGCAGTGGGCCGACTGCATTTGGCCA‑GTCGGCCGTTTCGCCTGCACCAGGGACAATGATCACCGCCCCTTGCGGATCGTTGTCGTTgg                                                                            <  5:190796/147‑1 (MQ=255)                                                                  gAGCAGGTCGGGCAGGCTGACGCTCAGGCTGTGCCAGCCGATATCAGGGAATTTTTGCCGCAGTGGGCCGACTGCATTTGGCCA‑GTCGGCCGTTTCGCCTGCACCAGGGACAATGATCACCGCCCCTTGCGGATCGTTGTCGTTGGCCg                                                                         >  5:107566/1‑149 (MQ=255)                                                                        gTCGGGCAGGCTGACGCTCAGGCTGTGCCAGCCGATATCAGGGAATTTTTGCCGCAGTGGGCCGACTGCGTTTGGCCA‑GTCGGCCGTTTCGCCTGCACCAGGGACAATGATCACCGCCCCTTGCGGATCGTTGTCGTTGGCCGGCtt                                                                     <  8:164680/147‑1 (MQ=255)                                                                        gTCGGGCAGGCTGACGCTCAGGCTGTGCCAGCCGATATCAGGGAATTTTTGCCGCAGTGGGCCGACTGCATTTGGCCA‑GTCGGCCGTTTCGCCTGCACCAGGGACAATGATCACCGCCCCTTGCGGAtc                                                                                       >  5:228130/1‑129 (MQ=255)                                                                        gTCGGGCAGGCTGACGCTCAGGCTGTGCCAGCCGATATCAGGGAATTTTTGCCGCAGTGGGCCGACTGCATTTGGCCA‑GTCGGCCGTTTCGCCTGCACCAGGGACAATGATCACCGCCCCTTGCGGAtc                                                                                       <  6:228130/129‑1 (MQ=255)                                                                        gTCGGGCAGGCTGACGCTCAGGCTGTGCCAGCCGATATCAGGGAATTTTTGCCGCAGTGGGCCGACTGCATTTGGCCA‑GTCGGCCGTTTCGCCTGCACCAGGGACAATGATCACCGCCCCTTGCGGATCGTTGTCGTTGGCCGGCTTc                                                                    >  7:303596/1‑148 (MQ=255)                                                                           gggCAGGCTGACGCTCAGGCTGTGCCAGCCGATATCAGGGAATTTTTGCCGCAGTGGGCCGACTGCATTTGGCCA‑GTCGGCCGTTTCGCCTGCACCAGGGACAATGATCACCGCCCCTTGCGGATCGTTGTCGTTGGCCGGCTTCca                                                                  >  1:178121/1‑147 (MQ=255)                                                                              cAGGCTGACGCTCAGGCTGTGCCAGCCGATATCAGGGAATTTTTGCCGCAGTGGGCCGACTGCATTTGGCCA‑GTCGGCCGTTTCGCCTGCACCAGGGACAATGATCACCGCCCCTTGCGGATCGTTGTCGTTGGCCGGCTTCCACAggg                                                             <  8:303596/149‑1 (MQ=255)                                                                               aGGCTGACGCTCAGGCTGTGCCAGCCGATATCAGGGAATTTTTGCCGCAGTGGGCCGACTGCATTTGGCCA‑GTCGGCCGTTTCGCCTGCACCAGGGACAATGATCACCGCCCCTTGCGGATCGTTGTCGTTGGCCGGCTTCCACAGGGc                                                            >  5:359760/1‑149 (MQ=255)                                                                               aGGCTGACGCTCAGGCTGTGCCAGCCGATATCAGGGAATTTTTGCCGCAGTGGGCCGACTGCATTTGGCCA‑GTCGGCCGTTTCGCCTGCACCAGGGACAATGATCACCGCCCCTTGCGGATCGTTGTCGTTGGCCGGCTTCCACAGGGc                                                            >  3:7569/1‑149 (MQ=255)                                                                                 gCTGACGCTCAGGCTGTGCCAGCCGATATCAGGGAATTTTTGCCGCAGTGGGCCGACTGCATTTGGCCA‑GTCGGCCGTTTCGCCTGCACCAGGGACAATGATCACCGCCCCTTGCGGATCGTTGTCGTTGGCCGGCTTCCACGGGGc                                                            >  1:235169/1‑147 (MQ=255)                                                                                  cTGACGCTCAGGCTGTGCCAGCCGATATCAGGGAATTTTTGCCGCAGTGGGCCGACTGCATTTGGCCA‑GTCGGCCGTTTCGCCTGCACCAGGGACAATGATCACCGCCCCTTGCGGATCGTTGTCGTTGGCCGGCTTCCACAGGGCCAg                                                         <  2:306854/149‑1 (MQ=255)                                                                                     aCGCTCAGGCTGTGCCAGCCGATATCAGGGAATTTTTGCCGCAGTGGGCCGACTGCATTTGGCCA‑GTCGGCCGTTTCGCCTGCACCAGGGACAATGATCACCGCCCCTTGCGGATCGTTGTCGTTGGCCGGCTTCCACAGGGCCAGGaa                                                      <  4:99669/149‑1 (MQ=255)                                                                                       gCTCAGGCTGTGCCAGCCGATATCAGGGAATTTTTGCCGCAGTGGGCCGACTGCATTTGGCCA‑GTCGGCCGTTTCGCCTGCACCAGGGACAATGATCACCGCCCCTTGCGGATCGTTGTCGTTGGCCGGCTTCCACAGGGCCAGGAAGc                                                    >  2:189104/1‑149 (MQ=255)                                                                                         tCAGGCTGTGCCAGCCGATATCAGGGAATTTTTGCCGCAGTGGGCCGACTGCATTTGGCCA‑GTCGGCCGTTTCGCCTGCACCAGGGACAATGATCACCGCCCCTTGCGGATCGTTGTCGTTGGCCGGCTTCCACAGGGCCAGGAAGCTg                                                  >  4:352242/1‑149 (MQ=255)                                                                                            ggCTGTGCCAGCCGATATCAGGGAATTTTTGCCGCAGTGGGCCGACTGCATTTGGCCA‑GTCGGCCGTTTCGCCTGCACCAGGGACAATGATCACCGCCCCTTGCGGATCGTTGTCGTTGGCCGGCTTCCACAGGGCCAGGAAGCTGTCg                                               <  6:107566/149‑1 (MQ=255)                                                                                             gCTGTGCCAGCCGATATCAGGGAATTTTTGCCGCAGTGGGCCGACTGCATTTGGCCA‑GTCGGCCGTTTc                                                                                                                              >  6:274747/1‑69 (MQ=255)                                                                                             gCTGTGCCAGCCGATATCAGGGAATTTTTGCCGCAGTGGGCCGACTGCATTTGGCCA‑GTCGGCCGTTTc                                                                                                                              <  5:274747/69‑1 (MQ=255)                                                                                               tgtgCCAGCCGATATCAGGGAATTTTTGCCGCAGTGGGCCGACTGCATTTGGCCACGTCGGCCGTTTCGCCTGCACCAGGGACAATGATCACCGCCCCTTGCGGATCGTTGTCGTTGGCCGGCTTCCACAGGGCCAGGAAGCTGTCgg                                              >  1:287659/1‑148 (MQ=255)                                                                                                     aGCCGATATCAGGGAATTTTTGCCGCAGTGGGCCGACTGCATTTGGCCA‑GTCGGCCGTTTCGCCTGCACCAGGGACAATGATCACCGCCCCTTGCGGATCGTTGTCGTTGGCCGGCTTCCACAGGGCCAGGAAGCTGTCGGCACCGGcc                                      >  4:241059/1‑149 (MQ=255)                                                                                                      gCCGATATCAGGGAATTTTTGCCGCAGTGGGCCGACTGTATTTGGCCA‑GTCGGCCGTTTCGCCTGCACCAGGGACAATGATCACCGCCCCTTGCGGATCGTTGTCGTTGGCCGGCTTCCACAGGGCCAGGAAGCTGTCGGCACCGGCCt                                     >  8:233651/1‑149 (MQ=255)                                                                                                      gCCGATATCAGGGAATTTTTGCCGCAGTGGGCCGACTGCATTTGGCCA‑GTCGGCCGTTTCGCCTGCACCAGGGACAATGATCACCGCCCCTTGCGGATCGTTGTCGTTGGCCGGCTTCCACAGGGCCAGGAAGCTGTCg                                               <  4:282289/139‑1 (MQ=255)                                                                                                      gCCGATATCAGGGAATTTTTGCCGCAGTGGGCCGACTGCATTTGGCCA‑GTCGGCCGTTTCGCCTGCACCAGGGACAATGATCACCGCCCCTTGCGGATCGTTGTCGTTGGCCGGCTTCCACAGGGCCAGGAAGCTGTCg                                               >  3:282289/1‑139 (MQ=255)                                                                                                      gCCGATATCAGGGAATTTTTGCCGCAGTGGGCCGACTGCATTTGGCCA‑GTCGGCCGTTTCGCCTGCACCAGGGACAATGATCACCGCCCCTTGCGGATCGTTGTCGTTGGCCGGCTTCCACAGGGCCAGGAAGCTGTCGGCACCGGCCt                                     >  4:63987/1‑149 (MQ=255)                                                                                                        cGATATCAGGGAATTTTTGCCGCAGTGGGCCGACTGCATTTGGCCA‑GTCGGCCGTTTCGCCTGCACCAGGGACAATGATCACCGCCCCTTGCGGATCGTTGTCGTTGGCCGGCTTCCACAGGGCCAGGAAGCTGTCGGCACCGGCCTg                                    >  6:235116/1‑148 (MQ=255)                                                                                                          atatCAGGGAATTTTTGCCGCAGTGGGCCGACTGCATTTGGCCA‑GTCGGCCGTTTCGCCTGCACCAGGGACAATGATCACCGCCCCTTGCGGATCGTTGTCGTTGGCCGGCTTCCACAGGGCCAGGAAGCTGTCGGCACCGGCCTGCAg                                 <  5:235116/149‑1 (MQ=255)                                                                                                          atatCAGGGAATTTTTGCCGCAGTGGGCCGACTGCATTTGGCCA‑GTCGGCCGTGGCGCCTGCACCAGGGACAATGATCACCGCCCCTTGCGGATCGTTGTCGTTGGCCGGCTTCCACAGGGCCAGGAAGCTGTCGGCACCGGCCTGCAg                                 <  3:122114/149‑1 (MQ=255)                                                                                                           tatCAGGGAATTTTTGCCGCAGTGGGCCGACTGCATTTGGCCA‑GTCGGCCGTTTCGCCTGCACCAGGGACAATGATCACCGCCCCTTGCGGATCGTTGTCGTTGGCCGGCTTCCACAGGGCCAGGAAGCTGTCGGCACCGGCCTGCAgg                                <  4:7569/149‑1 (MQ=255)                                                                                                              cAGGGAATTTTTGCCGCAGTGGGCCGACTGCATTTGGCCA‑GTCGGCCGTTTCGCCTGCACCAGGGACAATGATCACCGCCCCTTGCGGATCGTTGTCGTTGGCCGGCTTCCACAGGGCCAGGAAGCTGTCGGCACCGGCCTGCAGGGTc                             >  4:348738/1‑149 (MQ=255)                                                                                                               aGGGAATTTTTGCCGCAGTGGGCCGACTGCATTTGGCCA‑GTCGGCCGTTTCGCCTGCACCAGGGACAATGATCACAGCCCCTTGCGGATCGTTGTCGTTGGCCGGCTTCCACAGGGCCAGGAAGCTGTCGGCACCGGCCTGCAGGGTct                            >  4:143010/1‑149 (MQ=255)                                                                                                                     tttttGCCGCAGTGGGCCGACTGCATTTGGCCA‑GTCGGCCGTTTCGCCTGCACCAGGGACAATGATCAGCGCCCCTTGCGGATCGTTGTCGTTGGCCGGCTTCCACAGGGCCAGGAAGCTGTCGGCACCGGCCTGCAGGGTCTgctgct                      <  2:287659/149‑1 (MQ=255)                                                                                                                       tttGCCGCAGTGGGCCGACTGTATTTGGCCA‑GTCGGCCGTTTCGCCTGCACCAGGGACAATGATCACCGCCCCTTGCGGATCGTTGTCGTTGGCCGGCTTCCACAGGGCCAGGAAGCTGTCGGCACCGGCCTGCAGGGTCTGCTGCTc                     <  7:233651/148‑1 (MQ=255)                                                                                                                             gCAGTGGGCCGACTGCATTTGGCCA‑GTCGGCCGTTTCGCCTGCACCAGGGACAATGATCACCGCCCCTTGCGGATCGTTGTCGTTGGCCGGCTTCCACAGGGCCAGGAAGCTGTCGGCACCGGCCTGCAGGGTCTGCTGCTCAGCCTTg              <  2:178121/149‑1 (MQ=255)                                                                                                                                   ggCCGACTGCATTTGGCCA‑GTCGGCCGTTTCGCCTGCACCAGGGACAATGATCACCGCCCCTTGCGGATCGTTGTCGTTGGCCGGCTTCCACAGGGCCAGGAAGCTGTCGGCACCGGCCTGCAGGGTCTGCTGCTCAGCCTTGGgcagc        <  5:343916/149‑1 (MQ=255)                                                                                                                                   ggCCGACTGCATTTGGCCA‑GTCGGCCGTTTCGCCTGCACCAGGGACAATGATCACCGCCCCTTGCGGATCGTTGTCGTTGGCCGGCTTCCACAGGGCCAGGAAGCTGTCGGCACCGGCCTGCAGGGTCTGCTGCTCAGCCTTGGgcag         <  3:63987/148‑1 (MQ=255)                                                                                                                                    gCCGACTGCATTTGGCCA‑GTCGGCCGTTTCGCCTGCACCAGGGACAATGATCACCGCCCCTTGCGGATCGTTGTCGTTGGCCGGCTTCCACAGGGCCAGGAAGCTGTCGGCACCGGCCTGCAGGGTCTGCTGCTCAGCCTTGGgcag         >  1:14434/1‑147 (MQ=255)                                                                                                                                         cTGCATTTGGCCA‑GTCGGCCGTTTCGCCTGCACCAGGGACAATGATCACCGCCCCTTGCGGATCGTTGTCGTTGGCCGGCTTCCACAGGGCCAGGAAGCTGTCGGCACCGGCCTGCAGGGTCTGCTGCTCAGCCTTGGGCAGCAGCCGt  <  1:189104/149‑1 (MQ=255)                                                                                                                                         cTGCATTTGGCCA‑GTCGGCCGTTTCGCCTGCACCAGGGACAATGATCACCGCCCCTTGCGGATCGTTGTCGTTGGCCGGCTTCCACAGGGCCAGGAAGCTGTCGGCACCGGCCTGCAGGGTCTGCTGCTCAGCCTTGGGCAGCAGCCGt  <  3:348738/149‑1 (MQ=255)                                                                                                                                             |                                                                                                                                                  TCGCCTTTGTCTGGCGCAGGCGCTGCCGGTGGTGTGGCTTCGACCCGCGCTTGCGGGCTGTCTGCGAGCAGGTCGGGCAGGCTGACGCTCAGGCTGTGCCAGCCGATATCAGGGAATTTTTGCCGCAGTGGGCCGACTGCATTTGGCCA‑GTCGGCCGTTTCGCCTGCACCAGGGACAATGATCACCGCCCCTTGCGGATCGTTGTCGTTGGCCGGCTTCCACAGGGCCAGGAAGCTGTCGGCACCGGCCTGCAGGGTCTGCTGCTCAGCCTTGGGCAGCAGCCGT  >  NC_002947/495576‑495860

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑