Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,796,670 G→A 8.8% L410L (CTC→CTT)  gltB ← L‑glutamate synthase subunit alpha

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,796,6700GA 8.8% 126.4 / 2.9 45L410L (CTC→CTT) gltBL‑glutamate synthase subunit alpha
Reads supporting (aligned to +/- strand):  ref base G (19/22);  new base A (2/2);  total (21/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATGTATTGCTTGAGCTGGTCAGCGTCGTAGCTGGCCACACCCTGGTCGTCGGTCAGCGTCGCCTGGATGCGGGTGGCGTGCTGACGCAGCCAGCGCTTGTACGGGTGGC‑GCGACTTCAGGCGGTTGTCGATGGCGTCGGTGTCGAGGATCTGGCCGGTTTCGGTGTCCACGGCAAGGATCTGGCCCGGGCCGACACGGCCCTTGGCCAGCACTTCCTCAGGCTGATAGCCCCATACGCCGATTTCCGAGGCGATGGTGATGTAACCATTGGTGGTGGTCACCCAGCGCGCCG  >  NC_002947/5796526‑5796818
                                                                                                                                                 |                                                                                                                                                    
cctttATTGCTTGAGCTGGTCAGCGTCGTATCTGGCCACTCGCTGGTCGTCGGTGAGCGTGGCCTGGATGCGGGTGGCGTGCTGACGCAGCCAGCGGTTGTACGGGTGGC‑GCGACTTCAGGCGGTTGTCGATGGCGTCGGTGTCgt                                                                                                                                                     <  5:73905/142‑2 (MQ=255)
        gCTTGAGCTGGTCAGCGTCGTAGCTGGCCACACCCTGGTCGTCGGTCAGCGTCGCCTGGATGCGGGTGGCGTGCTGACGCAGCCAGCGCTTGTACGGGTGGC‑GCGACTTCAGGCGGTTGTCGATGGCGTCGGTGTCGAGGATCTGGCCg                                                                                                                                          >  8:239612/1‑149 (MQ=255)
        gCTTGAGCTGGTCAGCGTCGTAGCTGGCCACACCCTGGTCGTCGGTCAGCGTCGCCTGGATGCGGGTGGCGTGCTGACGCAGCCAGCGCTTGTACGGGTGGC‑GCGACTTCAGGCGGTTGTCGATGGCGTCGGTGTCGAGGATCTGGCCg                                                                                                                                          >  6:215988/1‑149 (MQ=255)
          ttGAGCTGGTCAGCGTCGTAGCTGGCCACACCCTGGTCGTCGGTCAGCGTCGCCTGGATGCGGGTGGCGTGCTGACGCAGCCAGCGCTTGTACGGGAGGC‑GCGACTTCAGGCGGTTGTCGATGGCGTCGGTGTCGAGGATCTGGCCGGt                                                                                                                                        >  5:61078/1‑149 (MQ=255)
              gCTGGTCAGCGTCGTAGCTGGCCACACCCTGGTCGTCGGTCAGCGTCGCCTGGATGCGGGTGGCGTGCTGACGCAGCCAGCGCTTGTACGGGTGGC‑GCGACTTCAGGCGGTTGTCGATGGCGTCGGTGTCGAGGATCTGGCCGGTTTCg                                                                                                                                    <  4:206180/149‑1 (MQ=255)
                  gTCAGCGTCGTAGCTGGCCACACCCTGGTCGTCGGTCAGCGTCGCCTGGATGCGGGTGGCGTGCTGACGCAGCCAGCGCTTGTACGGGTGGC‑GCGACTTCAGGCGGTTGTCGATGGCGTCTGTGTCGAGGATCTGGCCGGTTTCGgt                                                                                                                                  <  2:141403/147‑1 (MQ=255)
                     aGCGTCGTAGCTGGCCACACCCTGGTCGTCGGTCAGAGTCGCCTGGATGCGGGTGGCGTGCTGACGCAGCCAGCGCTTGTACGGGTGGC‑GCGACTTCAGGCGGTTGTCGATGGCGTCGGTGTCGAGGATCTGGCCGGTTTCGGTGTCCa                                                                                                                             >  1:52981/1‑149 (MQ=255)
                         tcgtAGCTGGCCACACCCTGGTCGTCGGTCAGCGTCGCCTGGATGCGGGTGGCGTGCTGACGCAGCCAGCGCTTGTACGGGTGGC‑GCGACTTCAGGCGGTTGTCGATGGCGTCGGTGTCGAGGATCTgg                                                                                                                                             <  4:310437/129‑1 (MQ=255)
                         tcgtAGCTGGCCACACCCTGGTCGTCGGTCAGCGTCGCCTGGATGCGGGTGGCGTGCTGACGCAGCCAGCGCTTGTACGGGTGGC‑GCGACTTCAGGCGGTTGTCGATGGCGTCGGTGTCGAGGATCTGGCCGGTTTCGGTGTCCACGGc                                                                                                                         <  7:239612/149‑1 (MQ=255)
                                tGGCCACACCCTGGTCGTCGGTCAGCGTCGCCTGGATGCGGGTGGCGTGCTGACGCAGCCAGCGCTTGTACGGGTGGC‑GCGACTTCAGGCGGTTGTCGATGGCGTCGGTGTCGAGGATCTGGc                                                                                                                                            <  8:285821/123‑1 (MQ=255)
                                tGGCCACACCCTGGTCGTCGGTCAGCGTCGCCTGGATGCGGGTGGCGTGCTGACGCAGCCAGCGCTTGTACGGGTGGC‑GCGACTTCAGGCGGTTGTCGATGGCGTCGGTGTCGAGGATCTGGc                                                                                                                                            >  7:285821/1‑123 (MQ=255)
                                 gccaacacCCTGGTCGTCGGTCACCGTCGCCTGGATGCGGGTGGCGTGCTGACCCAGCCAGCGCATCTACGCGTGGC‑GCGACTTCAGGCGCTTGTCGATGGCGACGGTGTCAAGGATCTGGCCGGATTCGGTCTCCACGGCAAGCATCt                                                                                                                 >  4:125170/5‑149 (MQ=255)
                                 gccaacacCATGGTCGTCGGTCACCGTCGCCTGGATGCGGGTGGCGTGCTGAAGCAGCCAGCGCTTGTACGGGTGGC‑GCGACTTCAGGCGGTTGTCGATGGCTACGGTGTCAAGGATCTGGACTGTTTCTGTTTCAACAGCAAGGATCt                                                                                                                 >  4:130660/5‑149 (MQ=255)
                                  gCCACACCCTGGTCGTCGGTCAGCGTCGCCTGGATGCGGGTGGCGTGCTGACGCAGCCAGCGCTTGTACGGGAGGC‑GCGACTTCAGGCGGTTGTCGATGGCGTCGGTGTCGAGGATCTGGCCGGTTTCGGTGTCCACGGCAAGGATc                                                                                                                  <  6:61078/147‑1 (MQ=255)
                                     acacCCTGGTCGTCGGTCAGCGTCGCCTGGATGCGGGTGGCGTGCTGACGCAGCCAGCGCTTGTACGGGTGGC‑GCGACTTCAGGCGGTTGTCGATGGCGTCGGTGTCGAGGATCTGGCCGGTTTCGGTGTCCACGGCAAGGAt                                                                                                                   >  3:97963/1‑143 (MQ=255)
                                     acacCCTGGTCGTCGGTCAGCGTCGCCTGGATGCGGGTGGCGTGCTGACGCAGCCAGCGCTTGTACGGGTGGC‑GCGACTTCAGGCGGTTGTCGATGGCGTCGGTGTCGAGGATCTGGCCGGTTTCGGTGTCCACGGCAAGGAt                                                                                                                   <  4:97963/143‑1 (MQ=255)
                                       acCCTGGTCGTCGGTCAGCGTCGCCTGGATGCGGGTGGCGTGCTGACGCAGCCAGCGCTTGTACGGGTGGC‑GCGACTTCAGGCGGTTGTCGATGGCGTCGGTGTCGAGGATCTGGCCGGTTTCGGTGTCCACGGCAAGGATCTGGCCCg                                                                                                           >  7:182774/1‑149 (MQ=255)
                                       acCCTGGTCGTCGGTCAGCGTCGCCTGGATGCGGGTGGCGTGCTGACGCAGCCAGCGCTTGTACGGGTGGC‑GCGACTTCAGGCGGTTGTCGATGGCGTCGGTGTCGAGGATCTGGCCGGTTTCGGTGTCCACGGCAAGGATCTGGCCCg                                                                                                           >  2:261950/1‑149 (MQ=255)
                                                gtcgGTCAGCGTCGCCTGGATGCGGGTGGCGTGCTGACGCAGCCAGCGCTTGTACGGGTGGC‑GCGACTTCAGGCGGTTGTCGATGGCGTCGGTGTCGAGGATCTGGCCGGTTTCGGTGTCCACGGCAAGGATCTGGCCCGGGCCGacac                                                                                                  >  7:113263/1‑149 (MQ=255)
                                                      cAGCGTCGCCTGGATGCGGGTGGCGTGCTGACGCAGCCAGCGCTTGTACGGGTGGC‑GCGACTTCAGGCGGTTGTCGATGGCGTCGGTGTCGAGGATCTGGCCGGTTTCGGTGTCCACGGCAAGGATCTGGCCCGGGCCGACACGGccc                                                                                             >  7:94766/1‑148 (MQ=255)
                                                      cAGCGTCGCCTGGATGCGGGTGGCGTGCTGACGCAGCCAGCGCTTGTACGGGTGGC‑GCGACTTCAGGCGGTTGTCGATGGCGTCGGTGTCGAGGATCTGGCCGGTTTCGGTGTCCACGGCAAGGATCTGGCCCGGGCCGACACGGccc                                                                                             <  8:94766/148‑1 (MQ=255)
                                                          gTCGCCTGGATGCGGGTGGCGTGCTGACGCAGCCAGCGCTTGTACGGGTGGC‑GCGACTTCAGGCGGTTGTCGATGGCGTCGGTGTCGAGGATCTGGCCGGTTTCGGTGTCCACGGCAAGGATCTGGCCCGGGCCGACACGGCCCTTGGc                                                                                        <  8:5855/149‑1 (MQ=255)
                                                                 ggATGCGGGTGGCGTGCTGACGCAGCCAGCGCTTGTACGGGTGGC‑GCGACTTCAGGCGGTTGTCGATGGCGTCGGTGTCGAGGATCTGGCCGGTTTCGGTGTCCACGGCAAGGATCTGGCCCGGGCCGACACGGCCCTTGGCCAGCACt                                                                                 >  7:294147/1‑149 (MQ=255)
                                                                      cGGGTGGCGTGCTGACGCAGCCAGCGCTTGTACGGGTGGC‑GCGACTTCAGGCGGTTGTCGATGGCGTCGGTGTCGAGGATCTGGCCGGTTTCGGTGTCCACGGCAAGGATCTGGCCCGGGCCGACACGGCCCTTGGCCAGCACTTCCt                                                                             <  5:215988/148‑1 (MQ=255)
                                                                       ggggggCGGGCGGACGCAGCCAGCGCTTGTACGGGTGGC‑GCGACTTCAGGCGGTTGTCGATGGCGTCGGTGTCGAGGATCTGGCCGGTTTCGGTGGCCACGGCAAGGATCTGGCCCGGGCCGACACGGCCCTTGGCCAGCACTTCCTCa                                                                           <  5:228322/149‑1 (MQ=255)
                                                                        ggTGGCGTGCTGACGCAGCCAGCGCTTGTACGGGTGGC‑GCGACTTCAGGCGGTTGTCGATGGCGTCGGTGTCGAGGATCTGGCCGGTTTCGGTGTCCACGGCAAGGATCTGGCCCGGGCCGACACGGCCCTTGGCCAGCACTTCCTCAg                                                                          >  7:339141/1‑149 (MQ=255)
                                                                         gTGGCGTGCTGACGCAGCCAGCGCTTGTACGGGTGGC‑GCGACTTCAGGCGGTTGTCGATGGCGTCGGTGTCGAGGATCTGGCCGGTTTCGGTGTCCACGGCAAGGATCTGGCCCGGGCCGACACGGCCCTTGGCCAGCACTTCCTCAgg                                                                         >  1:131795/1‑149 (MQ=255)
                                                                            gCGTGCTGACGCAGCCAGCGCTTGTACGGGTGGCAGCGACTTCAGGCGGTTGTCGATGGCGTCGGTGTCGAGGATCTGGCCGGTTTCGGTGTCCACGGCAAGGATCTg                                                                                                                >  1:238057/1‑108 (MQ=255)
                                                                            gCGTGCTGACGCAGCCAGCGCTTGTACGGGTGGC‑GCGACTTCAGGCGGTTGTCGATGGCGTCGGTGTCGAGGATCTGGCCGGTTTCGGTGTCCACGGCAAGGATCTg                                                                                                                <  2:238057/107‑1 (MQ=255)
                                                                               tGCTGACGCAGCCAGCGCTTGTACGGGTGGC‑GCGACTTCAGGCGGTTGTCGATGGCGTCGGTGTCGAGGATCTGGCCGGTTTCGGTGTCCACGGCAAGGATCTCGCCCGGGCCGACACGGCCCTTGGCCAGCACTTCCTCAGGCTGATa                                                                   <  1:261950/149‑1 (MQ=255)
                                                                                    aCGCAGCCAGCGCTTGTACGGGTGGC‑GCGACTTCAGGCGGTTGTCGATGGCGTCGGTGTCGAGGATCTGGCCGGTTTCGGTGTCCACGGCAAGGATCTGGCCCGGGCCGACACGGCCCTTGGCCAGCACTTCCTCAGGCTGATAGcc                                                                <  8:182774/147‑1 (MQ=255)
                                                                                        agccagcGCTTGTACGGGTGGC‑GCGACTTCAGGCGGTTGTCGATGGCGTCGGTGTCGAGGATCTGGCCGGTTTCGGTGTCCACGGCAAGGATCTGGCCCGGGCCGACACGGCCCTTGGCCa                                                                                      <  3:94512/121‑1 (MQ=255)
                                                                                        agccagcGCTTGTACGGGTGGC‑GCGACTTCAGGCGGTTGTCGATGGCGTCGGTGTCGAGGATCTGGCCGGTTTCGGTGTCCACGGCAAGGATCTGGCCCGGGCCGACACGGCCCTTGGCCa                                                                                      >  4:94512/1‑121 (MQ=255)
                                                                                             gagcTTGTACGGGTGGC‑GCGACTTCAGGCGGTTGTCGATGGCGTCGGTGTCGAGGATCTGGCCTGTTTCGGTGTCCACGAAAACGATCTGGCCCGGGCCGACACGGCCCTTGGCCAGCACGTCCTCAGGCTGATAGCCCCATACGCCgc                                                     >  7:237790/3‑148 (MQ=255)
                                                                                              cgcTTGTACGGGTGGC‑GCGACTTCAGGCGGTTGTCGATGGCGTCGGTGTCGAGGATCTGGCCGGTTTCGGTGTCCACGGCAAGGATCTGGCCCGGGCCGACACGGCCCTTGGCCAGCACTTCCTCAGGCTGATAGCCCCATACGCCGAt                                                    <  8:294147/149‑1 (MQ=255)
                                                                                                 ttGTACGGGTGGC‑GCGACTTCAGGCGGTTGTCGATGGCGTCGGTGTCGAGGATCTGGCCGGTTTCGGTGTCCACGGCAAGGATCTGGCCCGGGCCGACACGGCCCTTGGCCAGCACTTCCTCAGGCTGATAGCACAATACGCAGAttac                                                 >  3:76402/1‑147 (MQ=255)
                                                                                                      cGGGTGGC‑GCGACTTCAGGCGGTTGTCGATGGCGTCGGTGTCGAGGATCTGGCCGGTTTCGGTGTCCACGGCAAGGATCTGGCCCGGGCCGACACGGCCCTTGGCCAGCACTTCCTCAGGCTGATAGCCCCATACGCCGATTTCCGAg                                             <  8:113263/148‑1 (MQ=255)
                                                                                                             tctcgACTTCTGGCGGTTGTCTATGGCTTCGGTTTCGATTATCTGGGCGGTTTGGGTGTCCAGGGCAAGGATCTGGCGCGGGCCGACACGGGCCTTGGCCAGCACTTCCTCAGGCTGATAGCCCGATACTCCGATTTCCGAGGCGATgg                                      <  4:76402/146‑1 (MQ=255)
                                                                                                                 gACTTCAGGCGGTTGTCGATGGCGTCGGTGTCAAGGATCTGGCCGGTTTCGGTGTCCACGGCAAGGATCTGGCCCGGGCCGACACGGCCCTTGGCCAGCACTTCCTCAGGCTGATAGCCCCATACGCCGATTTCCGAGGCGATGGTGAt                                  <  3:130660/149‑1 (MQ=255)
                                                                                                                 gACTTCAGGCGGTTGTCGATGGCGTCGGTGTCAAGGATCTGGCCGGTTTCGGTGTCCACGGCAAGGATCTGGCCCGGGCCGACACGGCCCTTGGCCAGCACTTCCTCAGGCTGATAGCCCCATACGCCGATTTCCGAGGCGATGGTGAt                                  <  3:125170/149‑1 (MQ=255)
                                                                                                                    ttCAGGCGGTTGTCGATGGCGTCGGTGTCGAGGATCTGGCCGGTTTCGGTGTCCACGGCAAGGATCTGGCCCGGGCCGACACGGCCCTTGGCCAGCACTTCCTCAGGCTGATAGccc                                                               >  6:211489/1‑117 (MQ=255)
                                                                                                                             ttGTCGATGGCGTCGGTGTCGAGGATCTGGCCGGTTTCGGTGTCCACGGCAAGGATCTGGCCCGGGCCGACACGGCCCTTGGCCAGCACTTCCTCAGGCTGATAGCCCCATACGCGGATTTCCGAGGCGATGGTGAt                                  <  5:106275/137‑1 (MQ=255)
                                                                                                                             ttGTCGATGGCGTCGGTGTCGAGGATCTGGCCGGTTTCGGTGTCCACGGCAAGGATCTGGCCCGGGCCGACACGGCCCTTGGCCAGCACTTCCTCAGGCTGATAGCCCCATACGCGGATTTCCGAGGCGATGGTGAt                                  >  6:106275/1‑137 (MQ=255)
                                                                                                                                tCGATGGCGTCGGTGTCGAGGATCTGGCCGGTTTCGGTGTCCACGGCAAGGATCTGGCCCGGGCCGACACGGCCCTTGGCCAGCACTTCCTCAGGCTGATAGCCCCATACGCCGATTTCCGAGGCGATGGTGATGTAACCATtggtggt                   <  8:339141/149‑1 (MQ=255)
                                                                                                                                  gATGGCGTCGGTGTCGAGGATCTGGCCGGTTTCGGTGTCCACGGCAAGGATCTGGCCCGGGCCGACACGGCCCTTGGCCAGCACTTCCTCAGGCTGATAGCCCCATACGCCGATTTCCGAGGCGATGGTGATGTAACCATtggtggtg                  <  6:341127/148‑1 (MQ=255)
                                                                                                                                               tCGAGGATCTGGCCGGTTTCGGTGTCCACGGCAAGGATCTGGCCCGGGCCGACACGGCCCTTGGCCAGCACTTCCTCAGGCTGATAGCCCCATACGCCGATTTCCGAGGCGATGGTGATGTAACCATTGGTGGTGGTCACCCAgcgcgc    <  2:131795/149‑1 (MQ=255)
                                                                                                                                                 gAGGATCTGGCCGGTTTCGGTGTCCACGGCAAGGATCTGGCCCGGGCCGACACGGCCCTTGGCCAGCACTTCCTCAGGCTGATAGCCCCATACGCCGATTTCCGAGGCGATGGTGATGTAACCATtggtgg                    >  7:4772/1‑131 (MQ=255)
                                                                                                                                                 gAGGATCTGGCCGGTTTCGGTGTCCACGGCAAGGATCTGGCCCGGGCCGACACGGCCCTTGGCCAGCACTTCCTCAGGCTGATAGCCCCATACGCCGATTTCCGAGGCGATGGTGATGTAACCATtggtgg                    <  8:4772/131‑1 (MQ=255)
                                                                                                                                                 gAGGATCTGGCCGGTTTCGGTGTCCACGGCAAGGATCTGGCCCGGGCCGACACGGCCCTTGGCCAGCACTTCCTCAGGCTGATAGCCCCATACGCCGATTTCCGAGGCGATGGTGATGTAACCATTGGTGGTGGTCACCCAGCGCGCCg  >  7:59952/1‑149 (MQ=255)
                                                                                                                                                 gAGGATCTGGCCGGTTTCGGTGTCCACGGCAAGGATCTGGCCCGGGCCGACACGGCCCTTGGCCAGCACTTCCTCAGGCTGATAGCCCCATACGCCGATTTCCGAGGCGATGGTGATGTAACCATTGGTGGTGGTCACCCAGCGCGCCg  >  7:5440/1‑149 (MQ=255)
                                                                                                                                                 gAGGATCTGGCCGGTTTCGGTGTCCACGGCAAGGATCTGGCCCGGGCCGACACGGCCCTTGGCCAGCACTTCCTCAGGCTGATAGCCCCATACGCCGATTTCCGAGGCGATGGTGATGTAACCATTGGTGGTGGTCACCCAGCGCGCCg  >  5:180980/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
CATGTATTGCTTGAGCTGGTCAGCGTCGTAGCTGGCCACACCCTGGTCGTCGGTCAGCGTCGCCTGGATGCGGGTGGCGTGCTGACGCAGCCAGCGCTTGTACGGGTGGC‑GCGACTTCAGGCGGTTGTCGATGGCGTCGGTGTCGAGGATCTGGCCGGTTTCGGTGTCCACGGCAAGGATCTGGCCCGGGCCGACACGGCCCTTGGCCAGCACTTCCTCAGGCTGATAGCCCCATACGCCGATTTCCGAGGCGATGGTGATGTAACCATTGGTGGTGGTCACCCAGCGCGCCG  >  NC_002947/5796526‑5796818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑