Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,798,502 G→A 7.0% T454I (ACC→ATC)  PP_5077 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,798,5020GA 7.0% 167.0 / 2.1 57T454I (ACC→ATC) PP_5077hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (26/27);  new base A (2/2);  total (28/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.24e-01

TCTTGATTGCCGCAACCGCTGCGTCGCGGTTGGCGAAACTGCCGTAAGTCACGACGTACAGAGGCTTGCCCTGCAGGTTTTTCTTGAAGTAGCGATAGTCGCCACCCTGGGCCTTGACGTAAGCCTGGGCCGACGCCTCGGAGCTGGTACCAAGGATCTGCACCACATAATTGCCCGGCTTCTGTCCGGCATACCAGCCACTGTTGCCAGCGCCAGCGGCGGCTGGTTTTTCGGCAGCTTTGGCAGCCGGCTTGGCCACGGCGACCTGGGTCGGCGCTGGTGCTGGCTTGGCCG  >  NC_002947/5798356‑5798649
                                                                                                                                                  |                                                                                                                                                   
tCTTGATTGCCGCAACCGCTGCGTCGCGGTTGGCGAAACTGCCGTAAGTCACGACGTACAGAGGCTTGCCCTGCAGGTTTTTCTTGAAGTAGCGAAAGTCGCCACCCTGGGCCTTGACGTAAGCCTGGGCCGACGCCTCGGAGCTGGTa                                                                                                                                                   >  5:253468/1‑149 (MQ=255)
 cTTGATTGCCGCAACCGCTGCGTCGCGGTTGGCGAAACTGCCGTAAGTCACGACGTACAGAGGCTTGCCCTGCAGGTTTTTCTTGAAGTAGCGATAGTCGCCACCCTGGGCCTTGACGTAAGCCTGGGCCGACGCCTCGGAGCTGGTa                                                                                                                                                   <  3:319313/148‑1 (MQ=255)
  ttGATTGCCGCAACCGCTGCGTCGCGGTTGGCGAAACTGCCGTAAGTCACGACGTACAGAGGCTTGCCCTGCAGGTTTTTCTTGAAGTAGCGATAGTCGCCACCCTGGGCCTTGACGTAAGCCTGGGCCGACGCCTCGGAGCTGGTAc                                                                                                                                                  >  6:320190/1‑148 (MQ=255)
    gATTGCCGCAACCGCTGCGTCGCGGTTGGCGAAACTGCCGTAAGTCACGACGTACAGAGGCTTGCCCTGCAGGTTTTTCTTGAAGTAGCGATAGTCGCCACCCTGGGCCTTGACGTAAGCCTGGGCCGACGCCTCGGAGCTGGTAcc                                                                                                                                                 >  2:40991/1‑147 (MQ=255)
     aTTGCCGCAACCGCTGCGTCGCGGTTGGCGAAACTGCCGTAAGTCACGACGTACAGAGGCTTGCCCTGCAGGTTTTTCTTGAAGTAGCGATAGTCGCCACCCTGGGCCTTGACGTAAGCCTGCGCCGACGCCTCGGAGCTGGTACCAAg                                                                                                                                              <  1:9493/149‑1 (MQ=255)
      ttGCCGCAACCGCTGCGTCGCGGTTGGCGAAACTGCCGTAAGTCACGACGTACAGAGGCTTGCCCTGCAGGTTTTTCTTGAAGTAGCGATAGTCGCCACCCTGGGCCTTGACGTAAGCCTGGGCCGACGCCTCGGAGCTGGTACCAAg                                                                                                                                              <  5:267717/148‑1 (MQ=255)
         ccGCAACCGCTGCGTCGCGGTTGGCGAAACTGCCGTAAGTCACGACGTACAGAGGCTTGCCCTGCAGGTTTTTCTTGAAGTAGCGATAGTCGCCACCCTGGGCCTTGACGTAAGCCTGGGCCGACGCCTCGGAGCTGGTACCAAGGAt                                                                                                                                           <  1:367800/148‑1 (MQ=255)
              aCCGCTGCGTCGCGGTTGGCGAAACTGCCGTAAGTCACGACGTACAGAGGCTTGCCCTGCAGGTTTTTCTTGAAGTAGCGATAGTCGCCACCCTGGGCCTTGACGTAAGCCTGGGCCGACGCCTCGGAGCTGGTACCAAGGATCTGcac                                                                                                                                     <  5:71019/149‑1 (MQ=255)
               ccGCTGCGTCGCGGTTGGCGAAACTGCCGTAAGTCACGACGTACAGAGGCTTGCCCTGCAGGTTTTTCTTGAAGTAGCGATAGTCGCCACCCTGGGCCTTGACGTAAGCCTGGGCCGACGCCTCGGAGCTGGTACCAAGGATCTGcacc                                                                                                                                    <  6:305780/149‑1 (MQ=255)
                   tGCGTCGCGGTTGGCGAAACTGCCGTAAGTCACGACGTACAGAGGCTTGCCCTGAAGGTTTTTCTTGAAGTAGCGATAGTCGCCACCCTGGGCCTTGACGTAAGCCTGGGCCGACGCCTCGGAGCTGGTACCAAGGATCTGCACcaca                                                                                                                                 >  5:199121/1‑148 (MQ=255)
                     cGTCGCGGTTGGCGAAACTGCCGTAACACACGACGTACAGAGGCTTGCCCTGCAGGTTTTTCTTGAAGTAGCGATAGTCGCCACCCTGGGCCTTGACGTAAGCCTGGGCCGACGCCTCGGAGCTGGTACCAAGGATCTGCACCACATaa                                                                                                                              <  2:70219/149‑1 (MQ=255)
                           ggTTGGCGAAACTGCCGTAAGTCACGACGTACAGAGGCTTGCCCTGCAGGTTTTTCTTGAAGTAGCGATAGTCGCCACCCTGGGCCTTGACGTAAGCCTGGGCCGACGCCTCGGAGCTGGTACCAAGGATCTGCACCACATAATTGccc                                                                                                                        <  7:279381/149‑1 (MQ=255)
                                gCGAAACTGCCGTAAGTCACGACGTACAGAGGCTTGCCCTGCAGGTTTTTCTTGAAGTAGCGATAGTCGCCACCCTGGGCCTTGACGTAAGCCTGGGCCGACGCCTCGGAGCTGGTACCAAGGATCTGCACCACATAATTGCCCGGCtt                                                                                                                   <  4:324458/149‑1 (MQ=255)
                                    aaCTGCCGTAAGTCACGACGTACAGAGGCTTGCCCTGCAGGTTTTTCTTGAAGTAGCGACAGTCGCCACCCTGGGCCTTGACGTAAGCCTGGGCCGACGCCTCGGAGCTGGTACCAAGGATCTGCACCACATAATTGCCCGGCTTCTGt                                                                                                               <  6:253468/149‑1 (MQ=255)
                                         ccGTAAGGCACGACGTACAGAGGCTTGCCCTGCAGGTTTTTCTTGAAGTAGCGATAGTCGCCACCCTGGGCCTTGACGTAAGCCTGGGCCGACGCCTCGGAGCTGGTACCAAGGATCTGCACCACATAATTGCCCGGCTTCTGTCCg                                                                                                            <  4:193240/147‑1 (MQ=255)
                                          cGTAAGTCACGACGTACAGAGGCTTGCCCTGCAGGTTTTTCTTGAAGTAGCGATAGTCGCCACCCTGGGCCTTGACGTAAGCCTGGGCCGACGCCTCGGAGCTGGTACCAAGGATCTGc                                                                                                                                       <  4:363746/119‑1 (MQ=255)
                                          cGTAAGTCACGACGTACAGAGGCTTGCCCTGCAGGTTTTTCTTGAAGTAGCGATAGTCGCCACCCTGGGCCTTGACGTAAGCCTGGGCCGACGCCTCGGAGCTGGTACCAAGGATCTGc                                                                                                                                       >  3:363746/1‑119 (MQ=255)
                                                tCACGACGTACAGAGGCTTGCCATGCAGGTTTTTCTTGAAGTAGCGATAGTCGCCACCCTGGGCCTTGACGTAAGCCTGGGCCGCAGCCTCGGAGGTGGTACCAAGGATCTGCACAACATAATTGCCCGGCTTCTGTCCGGCATACGAg                                                                                                   >  8:144189/1‑149 (MQ=255)
                                                         aCAGAGGCTTGCCCTGCAGGTTTTTCTTGAAGTAGCGATAGTCGCCACCCTGGGCCTTGACGTAAGCCTGGGCCGACGCCTCGGAGCTGGTACCAAGGATCTGCACCACATAATTGCCCGGCTTCTGTCCGGCATACCAGCCACTGTTg                                                                                          >  8:179634/1‑149 (MQ=255)
                                                          cAGAGGCTTGCCCTGCAGGTTTTTCTTGAAGTAGCGATAGTCGCCACCCTGGGCCTTGACGTAAGCCTGGGCCGACGCCTCGGAGCTGGTACCAAGGATCTGCACCACATAATTGCCCGGCTTCTGTCCGGCATACCAGCCACTGTTg                                                                                          <  7:101821/148‑1 (MQ=255)
                                                           agagGCTTGCCCTGCAGGTTTTTCTTGAAGTAGCGATAGTCGCCACCCTGGGCCTTGACGTAAGCCTGGGCCGACGCCTCGGAGCTGGTACCAAGGATCTGCACCACATAATTGCCCGGCTTCTGTCCGGCATACCAGCCACTGTTgc                                                                                         >  3:310870/1‑148 (MQ=255)
                                                           agagGCTTGCCCTGCAGGTTTTTCTTGAAGTAGCGATAGTCGCCACCCTGGGCCTTGACGTAAGCCTGGGCCGACGCCTCGGAGCTGGTACCAAGGATCTGCACCACATAATTGCCCGGCTTCTGTCCGGCATACCAGCCACTGTTgc                                                                                         >  7:60067/1‑148 (MQ=255)
                                                                 ttGCCCTGCAGGTTTTTCTTGAAGTAGCGATAGTCGCCACCCTGGGCCTTGACGTAAGCCTGGGCCGACGCCTCGGAGCTGGTACCAAGGATCTGCACCACATAATTGCCCGGCTTCTGTCCGGCATACCAGCCACTGtt                                                                                           <  5:351255/140‑1 (MQ=255)
                                                                 ttGCCCTGCAGGTTTTTCTTGAAGTAGCGATAGTCGCCACCCTGGGCCTTGACGTAAGCCTGGGCCGACGCCTCGGAGCTGGTACCAAGGATCTGCACCACATAATTGCCCGGCTTCTGTCCGGCATACCAGCCACTGtt                                                                                           >  6:351255/1‑140 (MQ=255)
                                                                         cAGGTTTTTCTTGAAGTAGCGATAGTCGCCACCCTGGGCCTTGACGTAAGCCTGGGCCGACGCCTCGGAGCTGGTACCAAGGATCTGCACCACATAATTGCCCGGCTTCTGTCCGGCATACCAGCCACTGTTGCCAGCGCCAGcggcgg                                                                          <  3:351894/149‑1 (MQ=255)
                                                                            gTTTTTCTTGAAGTAGCGATAGTCGCCACCCTGGGCCTTGACGTAAGCCTGGGCCGACGCCTCGGAGCTGGTACCAAGGATCTGCACCACATAATTGCCCGGCTTCTGTCCGGCATACCAGCCACTGTTGCCAGCGCCAGCGGCGGCTg                                                                       >  5:272703/1‑149 (MQ=255)
                                                                            gTTTTTCTTGAAGTAGCGATAGTCGCCACCCTGGGCCTTGACGTAAGCCTGGGCCGACGCCTCGGAGCTGGTACCAAGGATCTGCACCACATAATTGCCCGGCTTCTGTCCGGCATACCAGCCACTGTTGCCAGCGCCAGCGGCGGCTg                                                                       >  8:91167/1‑149 (MQ=255)
                                                                                ttCTTGAAGTAGCGATAGTCGCCACCCTGGGCCTTGACGTAAGCCTGGGCCGACGCCTCGGAGCTGGTACCAAGGATCTGCACCACATAATTGCCCGGCTTCTGTCCGGCATACCAGCCACTGTTGCCAGCGCCAGCGGCGGCTGGtt                                                                    <  1:40991/148‑1 (MQ=255)
                                                                                   ttGAAGTAGCGTTAGGCGCCACCCTGGGCCTTGACGTAAGCCTGGGCCGACGCCTCGGAGCTGGTACCAAGGATCTGCACCACATAATTGCCCGGCtt                                                                                                                   <  8:303704/98‑1 (MQ=255)
                                                                                   ttGAAGTAGCGATAGTCGCCACCCTGGGCCTTGACGTAAGCCTGGGCCGACGCCTCGGAGCTGGTACCAAGGATCTGCACCACATAATTGCCCGGCTTCTGTCCGGCATACCAGCCACTGTTGCCAGCGCCAGCGGCGGCTGGTTTTTc                                                                >  4:31913/1‑149 (MQ=255)
                                                                                   ttGAAGTAGCGATAGTCGCCACCCTGGGCCTTGACGTAAGCCTGGGCCGACGCCTCGGAGCTGGTACCAAGGATCTGCACCACATAATTGCCCGGCTTCTGTCCGGCATACCAGCCACTGTTGCCAGCGCCAGCGGCGGCTGGTTTTTc                                                                >  4:47309/1‑149 (MQ=255)
                                                                                   ttGAAGTAGCGATAGTCGCCACCCTGGGCCTTGACGAAAGCCTGGGCCGACGCCTCGGAGCTGGTACCAAGGATCTGCACCACATAATTGCCCGGCtt                                                                                                                   >  7:303704/1‑98 (MQ=255)
                                                                                     gAAGTAGCGATAGTCGCCACCCTGGGCCTTGACGTAAGCCTGGGCCGACGCCTCGGAGCTGGTACCAAGGATCTGCACCACATAATTGCCCGGCTTCTGTCCGGCATACCAGCCACTGTTGCCAGCGCCAGCGGCGGCTGGTTTTTc                                                                <  8:60067/147‑1 (MQ=255)
                                                                                           gCGATAGTCGCCACCCTGGGCCTTGACGTAAGCCTGGGCCGACGCCTCGGAGCTGGTACCAAGGATCTGCACCACATAATTGCCCg                                                                                                                       >  3:325538/1‑86 (MQ=255)
                                                                                           gCGATAGTCGCCACCCTGGGCCTTGACGTAAGCCTGGGCCGACGCCTCGGAGCTGGTACCAAGGATCTGCACCACATAATTGCCCg                                                                                                                       <  4:325538/86‑1 (MQ=255)
                                                                                           gCGATAGTCGCCACCCTGGGCCTTGACGTAAGCCTGGGCCGACGCCTCGGAGCTGGTACCAAGGATCTGCACCACATAATTGCCCGGCTTCTGTCCGGCATACCAGc                                                                                                  <  2:23235/107‑1 (MQ=255)
                                                                                           gCGATAGTCGCCACCCTGGGCCTTGACGTAAGCCTGGGCCGACGCCTCGGAGCTGGTACCAAGGATCTGCACCACATAATTGCCCGGCTTCTGTCCGGCATACCAGc                                                                                                  >  1:23235/1‑107 (MQ=255)
                                                                                           gCGATAGTCGCCACCCTGGGCCTTGACGTAAGCCTGGGCCGACGCCTCGGAGCTGGTACCAAGGATCTGCACCACATAATTGCCCGGCTTCTGTCCGGCATACCAGCCACTGTTGCCAGCGCCAGCGGCGGCTGGTTTTTCGGCAGcat                                                        >  7:146181/1‑147 (MQ=255)
                                                                                           gCGATAGTCGCCACCCTGGGCCTTGACGTAAGCCTGGGCCGACGCCTCGGAGCTGATACCAAGGATCTGCACCACATAATTGCCCGGCTTCTGTCCGGCATACCAGCCACTGTTgccagc                                                                                     >  3:312137/1‑120 (MQ=255)
                                                                                           gCGATAGTCGCCACCCTGGGCCTTGACGTAAGCCTGGGCCGACGCCTCGGAGCTGATACCAAGGATCTGCACCACATAATTGCCCGGCTTCTGTCCGGCATACCAGCCACTGTTgccagc                                                                                     >  3:317410/1‑120 (MQ=255)
                                                                                           gCGATAGTCGCCACCCTGGGCCTTGACGTAAGCCTGGGCCGACGCCTCGGAGCTGATACCAAGGATCTGCACCACATAATTGCCCGGCTTCTGTCCGGCATACCAGCCACTGTTgccagc                                                                                     <  4:312137/120‑1 (MQ=255)
                                                                                           gCGATAGTCGCCACCCTGGGCCTTGACGTAAGCCTGGGCCGACGCCTCGGAGCTGATACCAAGGATCTGCACCACATAATTGCCCGGCTTCTGTCCGGCATACCAGCCACTGTTgccagc                                                                                     <  4:317410/120‑1 (MQ=255)
                                                                                             gATAGTCGCCACCCTGGGCCTTGACGTAAGCCTGGGCCGACGCCTCGGAGCTGGTACCAAGGATCTGCACCACATAATTGCCCGGCTTCTGTCCGGCATACCAGCCACTGTTGCCAGCGCCAGCGGCGGCTGGTTTTTc                                                                <  6:34205/139‑1 (MQ=255)
                                                                                             gATAGTCGCCACCCTGGGCCTTGACGTAAGCCTGGGCCGACGCCTCGGAGCTGGTACCAAGGATCTGCACCACATAATTGCCCGGCTTCTGTCCGGCATACCAGCCACTGTTGCCAGCGCCAGCGGCGGCTGGTTTTTc                                                                >  5:34205/1‑139 (MQ=255)
                                                                                             gATAGTCGCCACCCTGGGCCTTGACGTAAGCCTGGGCCGACGCCTCGGAGCTGGTACCAAGGATCTGCACCACATAATTGCCCGGCTTCTGTCCGGCATACCAGCCACTGTTGCCAGCGCCAGCGGCGGCTGGTTTTTCGGCAGCTTTg                                                      >  2:206438/1‑149 (MQ=255)
                                                                                                         ccTGGGCCTTGACGTAAGCCTGGGCCGACGCCTCGGAGCTGGTACCAAGGATCTGCACCACATAATTGCCCGGCTTCTGTCCGGCATAc                                                                                                      >  3:207508/1‑89 (MQ=255)
                                                                                                         ccTGGGCCTTGACGTAAGCCTGGGCCGACGCCTCGGAGCTGGTACCAAGGATCTGCACCACATAATTGCCCGGCTTCTGTCCGGCATAc                                                                                                      >  7:63417/1‑89 (MQ=255)
                                                                                                         ccTGGGCCTTGACGTAAGCCTGGGCCGACGCCTCGGAGCTGGTACCAAGGATCTGCACCACATAATTGCCCGGCTTCTGTCCGGCATAc                                                                                                      <  4:207508/89‑1 (MQ=255)
                                                                                                         ccTGGGCCTTGACGTAAGCCTGGGCCGACGCCTCGGAGCTGGTACCAAGGATCTGCACCACATAATTGCCCGGCTTCTGTCCGGCATAc                                                                                                      <  8:63417/89‑1 (MQ=255)
                                                                                                           tGGGCCTTGACGTAAGCCTGGGCCGACGACTCGGAGCTGGTACCAAGGATCTGCACCACATAATTGCCCGGCTTCTGTCCGGCATACCAGCCACTGTTGCCAGCGCCAGCGGCGGCTGGTTTTTCGGCAGCTTTGGCAGCCGGCTTGGc                                        >  2:304738/1‑149 (MQ=255)
                                                                                                                cTTGACGTAAGCCTGGGCCGACGCCTCGGAGCTGGTACCAAGGATCTGCACCACATAATTGCCCGGCTTCTGTCCGGCATACCAGCCACTGTTGCCAGCGCCAGCGGCGGCTGGTTTTTc                                                                >  3:227534/1‑120 (MQ=255)
                                                                                                                cTTGACGTAAGCCTGGGCCGACGCCTCGGAGCTGGTACCAAGGATCTGCACCACATAATTGCCCGGCTTCTGTCCGGCATACCAGCCACTGTTGCCAGCGCCAGCGGCGGCTGGTTTTTc                                                                <  4:227534/120‑1 (MQ=255)
                                                                                                                    aCGTAAGCCTGGGCCTACGCCTCGGAGCTGGTACCAAGGATCTGCACCACATAATTGCCCGGCTTCTGTCCGGCATACCAGCCACTGTTGCCAGCGCCAGCGGCGGCTGGTTTTTCGGCAGCTTTGGCAGCCGGCTTGGCCACGGCGa                                <  4:310870/148‑1 (MQ=255)
                                                                                                                             tGGGCCGACGCCTCGGAGCTGGTACCAAGGATCTGCACCACATAATTGCCCGGCTTCTGTCCGGCa                                                                                                         >  3:275061/1‑66 (MQ=255)
                                                                                                                             tGGGCCGACGCCTCGGAGCTGGTACCAAGGATCTGCACCACATAATTGCCCGGCTTCTGTCCGGCa                                                                                                         <  4:275061/66‑1 (MQ=255)
                                                                                                                                       ccTCGTAGCTGGTACCAAGTATCTGCACCACATAATTGCCGGGCTTCTGTCCGGCATACCAGCCACTGTTGCCAGCGCCAGCGGCGGCTGGTTTTTCGGCAGCTTTGGCAGCCGGCTTGTCCACGGCGACCTGGGTCGGCGCTGGTGCt            <  8:146181/149‑1 (MQ=255)
                                                                                                                                         tCGGAGCTGGTACCAAGGATCTGCACCACATAATTGCCCGGCTTCTGTCCGGCATACCAGCCACTGTTGCCAGCGCCAGCGGCGGCTGGTTTTTCGGCAGCTTTGGCAGCCGGCTTGGCCACGGCGACCTGGGTCGGCGCTGGTGCTgg          >  7:36851/1‑149 (MQ=255)
                                                                                                                                                 ggTACCAAGGATCTGCACCACATAATTGCCCGGCTTCTGTCCGGCATACCAGCCACTGTTGCCAGCGCCAGCGGCGGCTGGTTTTTCGGCAGCTTTGGCAGCCGGCTTGGCCACGGCGACCTGGGTCGGCGCTGGTGCTGGCTTGGCCg  >  7:122672/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
TCTTGATTGCCGCAACCGCTGCGTCGCGGTTGGCGAAACTGCCGTAAGTCACGACGTACAGAGGCTTGCCCTGCAGGTTTTTCTTGAAGTAGCGATAGTCGCCACCCTGGGCCTTGACGTAAGCCTGGGCCGACGCCTCGGAGCTGGTACCAAGGATCTGCACCACATAATTGCCCGGCTTCTGTCCGGCATACCAGCCACTGTTGCCAGCGCCAGCGGCGGCTGGTTTTTCGGCAGCTTTGGCAGCCGGCTTGGCCACGGCGACCTGGGTCGGCGCTGGTGCTGGCTTGGCCG  >  NC_002947/5798356‑5798649

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑