Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,825,223 C→G 6.1% A84P (GCT→CCT) ‡ trmB ← tRNA (guanosine(46)‑N7)‑methyltransferase TrmB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,825,2230CG 6.1% 198.9 / 2.2 62A84P (GCT→CCT) ‡trmBtRNA (guanosine(46)‑N7)‑methyltransferase TrmB
Reads supporting (aligned to +/- strand):  ref base C (29/29);  new base G (2/2);  total (31/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACGCAGCGGTTCAGCACTTCGATGGCATCGCAGTCATACACCCGCAGGTTTTTCAGCCCCTGGGTCAGCACGCCATTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTGGTCGTAGTCCACCGGGCTATCAGCCAGTGGCAGGATGTACAGCGGGCCGCCTTGGTCCAGACCGCGTTGCT  >  NC_002947/5825081‑5825369
                                                                                                                                              |                                                                                                                                                  
aCGCAGCGGTTCAGCACTTCGATGGCATCGCAGTCATACACCCGCAGGTTTTTCAGCCCCTGGGTCAGCACGCCATTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCAt                                                                                                                                              <  4:78782/149‑1 (MQ=255)
   cAGCGGTTCAGCACTTCGATGGCATCGCAGTCATACACCCGCAGGTTTTTCAGCCCCTGGGTCAGCACGCCATTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTc                                                                                                                                           <  7:342982/149‑1 (MQ=255)
      cGGTTCAGCACTTCGATGGCATCGCAGTCATACACCCGCAGGTTTTTCAGCCCCTGGGTCAGCACGCCATTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCcag                                                                                                                                        <  2:68968/149‑1 (MQ=255)
        gTTCAGCACTTCGATGGCATCGCAGTCATACACCCGCAGGTTTTTCAGCCCCTGGGTCAGCACGCCATTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCcagca                                                                                                                                      <  1:158806/149‑1 (MQ=255)
        gTTCAGCACTTCGATGGCATCGCAGTCATACACCCGCAGGTTTTTCAGCCCCTGGGTCAGCACGCCATTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCcagca                                                                                                                                      <  4:12560/149‑1 (MQ=255)
           cAGCACTTCGATGGCATCGCAGTCATACACCCGCAGGTTTTTCAGCCCCTGGGTCAGCACGCCATTGAGCAGCGCGCCAACACCCGTGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAgg                                                                                                                                    >  4:241132/1‑148 (MQ=255)
            aGCACTTCGATGGCATCGCAGTCATACACCCGCAGGTTTTTCAGCCCCTGGGTCAGCATGCCATTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCATGGGCCATTCCCTGCTgga                                                                                                                                   >  4:193126/1‑147 (MQ=255)
              cACTTCGATGGCATCGCAGTCATACACCCGCAGGTTTTTCAGCCCCTGGGTCAGCACGCCATTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAAt                                                                                                                                >  2:133603/1‑149 (MQ=255)
               aCTTCGATGGCATCGCAGTCATACACCCGCAGGTTTTTCAGCCCCTGGGTCAGCACGCCATTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATg                                                                                                                               >  1:40357/1‑149 (MQ=255)
                    gATGGCATCGCAGTCATACACCCGCAGGTTTTTCAGCCCCTGGGTCAGCACGCCATTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCa                                                                                                                          >  7:9598/1‑149 (MQ=255)
                    gATGGCATCGCAGTCATACACCCGCAGGTTTTTCAGCCCCTGGGTCAGCACGCCATTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCa                                                                                                                          <  2:274339/149‑1 (MQ=255)
                    gATGGCATCGCAGTCATACACCCGCAGGTTTTTCAGCCCCTGGGTCAGCACGCCATTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCa                                                                                                                          >  2:25733/1‑149 (MQ=255)
                     aTGGCATCGCAGTCATACACCCGCAGGTTTTTCAGCCCCTGGGTCAGCACGCCATTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCAt                                                                                                                         <  1:25733/149‑1 (MQ=255)
                       ggCATCGCAGTCATACACCCGCAGGTTTTTCAGCCCCTGGGTCAGCACGCCATTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGc                                                                                                                       >  2:31283/1‑149 (MQ=255)
                        gCATCGCAGTCATACACCCGCAGGTTTTTCAGCCCCTGGGTCAGCATGCCATTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCATGGGCCATTCCCTGCTGGAAATGGccgctgc                                                                                                                       <  3:193126/148‑6 (MQ=38)
                           tCGCAGTCATACACCCGCAGGTTTTTCAGCCCCTGGGTCAGCACGCCATTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGaa                                                                                                                   <  4:257115/149‑1 (MQ=255)
                                 tCATACACCCGCAGGTTTTTCAGCCCCTGGGTCAGCACGCCATTGAGCAGCGCGCCAACACCCGTGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGAt                                                                                                             <  3:241132/149‑1 (MQ=255)
                                 tCATACACCCGCAGGTTTTTCAGCCCCTGGGTCAGCACGCCATTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCGCATGCCGAAGCCGAt                                                                                                             >  3:255222/1‑149 (MQ=255)
                                 tCATACACCCGCAGGTTTTTCAGCCCCTGGGTCAGCACGCCATTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGAt                                                                                                             >  1:339229/1‑149 (MQ=255)
                                  cATACACCCGCAGGTTTTTCAGCCCCTGGGTCAGCACGCCATTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGAt                                                                                                             <  2:171764/148‑1 (MQ=255)
                                    tACACCCGCAGGTTTTTCAGCCCCTGGGTCAGCACGCCATTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGAtctc                                                                                                          >  1:68220/1‑149 (MQ=255)
                                      cacCCGCAGGTTTTTCAGCCCCTGGGTCAGCACGCCATTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTcc                                                                                                         <  7:183557/148‑1 (MQ=255)
                                        cccGCAGGTTTTTCAGCCCCTGGGTCAGCACGCCATTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAg                                                                                                      <  1:53354/149‑1 (MQ=255)
                                        cccGCAGGTTTTTCAGCCCCTGGGTCAGCACGCCATTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAg                                                                                                      <  1:31283/149‑1 (MQ=255)
                                         ccGCAGGTTTTTCAGCCCCTGGGTCAGCACGCCATTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTc                                                                                                                                           >  6:47986/1‑111 (MQ=255)
                                         ccGCAGGTTTTTCAGCCCCTGGGTCAGCACGCCATTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTc                                                                                                                                           <  5:47986/111‑1 (MQ=255)
                                           gCAGGTTTTTCAGCCCCTGGGTCAGCACGCCATTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTg                                                                                                   >  6:98641/1‑149 (MQ=255)
                                             aGGTTTTTCAGCCCCTGGGTCAGCACGCCATTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCAttcc                                                                                                                                           >  3:295822/1‑105 (MQ=255)
                                             aGGTTTTTCAGCCCCTGGGTCAGCACGCCATTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCAttcc                                                                                                                                           <  4:295822/107‑3 (MQ=255)
                                             aGGTTTTTCAGCCCCTGGGTCAGCACGCCATTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAAt                                                                                                                                >  6:75542/1‑118 (MQ=255)
                                             aGGTTTTTCAGCCCCTGGGTCAGCACGCCATTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAAt                                                                                                                                <  5:75542/118‑1 (MQ=255)
                                                tttttCAGCCCCTGGGTCAGCACGCCATTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCgg                                                                                              <  2:68220/149‑1 (MQ=255)
                                                   ttCAGGCCCTGGGTCAGCACGCCATTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGGCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCgggg                                                                                            <  2:40357/148‑1 (MQ=255)
                                                   ttCAGCCCCTGGGTCAGCACGCCATTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCgggg                                                                                            <  8:181080/148‑1 (MQ=255)
                                                       gCCCCTGGGTCAGCACGCCATTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGggccgaa                                                                                       >  1:219523/1‑149 (MQ=255)
                                                       gCCCCTGGGTCAGCACGCCATTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGggccgaa                                                                                       >  7:308113/1‑149 (MQ=255)
                                                             gggTCAGCACGCCATTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGggccgaacggccg                                                                                 <  7:332517/149‑1 (MQ=255)
                                                                tCAGCACGCCATTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCATGGGCCATTCCCTGCTGGAAATGGCCGCTGCCgcac                                                                                                                  >  6:369574/1‑110 (MQ=25)
                                                                tCAGCACGCCATTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCATGGGCCATTCCCTGCTGGAAATGGCCGCTGCCgcac                                                                                                                  <  5:369574/113‑4 (MQ=25)
                                                                      cGCCATTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTgg                                                                        <  8:145008/149‑1 (MQ=255)
                                                                          aTTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTGGTCg                                                                     >  3:283352/1‑148 (MQ=255)
                                                                                 agcGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTGGTCGTAGTCCAc                                                             <  1:133603/149‑1 (MQ=255)
                                                                                         aaCACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTGGTCGTAGTCCACCGGGCTAt                                                     >  7:213439/1‑149 (MQ=255)
                                                                                          acacCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTGGTCGTAGTCCACCGGGCTAt                                                     >  3:61661/1‑148 (MQ=255)
                                                                                          acacCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTGGTCGTAGTCCACCGGGCTATc                                                    <  2:339229/149‑1 (MQ=255)
                                                                                             cccGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTGGTCGTAGTCCACCGGGCTATAAGc                                                 >  8:232996/1‑149 (MQ=255)
                                                                                              ccGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTGGTCGTAGTCCACCGGGCTATCAGc                                                 >  6:217232/1‑148 (MQ=255)
                                                                                              ccGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTGGTCGTAGTCCACCGGGCTATCAGc                                                 >  4:352788/1‑148 (MQ=255)
                                                                                                     gTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTGGTCGTAGTCCACCGGGCTATCAGCCAGTGGCa                                         <  5:217232/149‑1 (MQ=255)
                                                                                                       gCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTGGTCGTAGTCCACCGGGCTATCAGCCAGTGGCAg                                        >  5:193963/1‑148 (MQ=255)
                                                                                                           ttCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTGGTCGTAGTCCACCGGGCTATCAGCCAGTGGCAGGATg                                    >  2:173192/1‑148 (MQ=255)
                                                                                                           ttCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGACGAACACCTGGTCGTAGTCCACCGGGCTATCAGCCAGTGGCatgatg                                    >  5:219735/1‑148 (MQ=255)
                                                                                                               acacCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTGGTCGTAGTCCACCGGGCTATCAGCCAGTGGCAGGATGTACAg                               >  5:252870/1‑149 (MQ=255)
                                                                                                                  ccGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCAAACACCTGGTCGTAGTCCACCGGGCTATCAGCCAGTGGCAGGATGTACAGCgg                            <  8:9598/149‑1 (MQ=255)
                                                                                                                     aTGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTGGTCGTAGTCCACCGGGCTATCAGCCAGTGGCAGGATGTACAGCGGgcc                         >  7:326073/1‑149 (MQ=255)
                                                                                                                           tCCTGCTCAGGTGCGGGAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTGGTCGTAGTCCACCGGGCTATCAGCCAGTGGCAGGATGTACAGCGGGCCGCCTTg                   <  5:98641/149‑1 (MQ=255)
                                                                                                                              tGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTGGTCGTAGTCCACCGGGCTATCAGCCAGTGGCAGGATGTACAGCGGGCCGCCTTGGTc                >  1:238628/1‑149 (MQ=255)
                                                                                                                               gCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTGGTCGTAGTCCACCGGGCTATCAGCCAGTGGCAGGATGTACAGCGGGCCGCCTTGGTc                >  5:57831/1‑148 (MQ=255)
                                                                                                                               gCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTGGTCGTAGTCCACCGGGCTATCAGCCAGTGGCAGGATGTACAGCGGGCCGCCTTGGTc                >  2:103951/1‑148 (MQ=255)
                                                                                                                                    ggTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTGGTCGTAGTCCACCGGGCTATCAGCCAGTGGCAGGATGTACAGCGGGCCGCCTTGGTCCAGAcc          <  2:219523/149‑1 (MQ=255)
                                                                                                                                     gTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTGGTCGTAGTCCACCGGGCTATCAGCCAGTGGCAGGATGTACAGCGGGCCGCCTTGGTCCAGACcg         <  4:61661/149‑1 (MQ=255)
                                                                                                                                            aGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTGGTCGTAGTCCACCGGGCTATCAGCCAGTGGCAGGATGTACAGCGGGCCGCCTTGGTCCAGACCGCGTTGCt  <  6:193963/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                  
ACGCAGCGGTTCAGCACTTCGATGGCATCGCAGTCATACACCCGCAGGTTTTTCAGCCCCTGGGTCAGCACGCCATTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTGGTCGTAGTCCACCGGGCTATCAGCCAGTGGCAGGATGTACAGCGGGCCGCCTTGGTCCAGACCGCGTTGCT  >  NC_002947/5825081‑5825369

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑