Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,838,213 G→A 6.8% V338V (GTG→GTA)  PP_5116 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,838,2130GA 6.8% 176.5 / 2.6 59V338V (GTG→GTA) PP_5116hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (24/31);  new base A (2/2);  total (26/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.19e-01

GTTTCTGGCCCTGGCCTTGGCAAGCGCTGTTCTGGGAGAGGGCCTCGAGTCGCGCTTGATGGTGGAGTTGCGCCAACGACGCGGCCTGACCTACGGCGTGCACACGCATGTATTGCCATTGAGTGCAGGCGGCCTGTTCACCGTGGAATGGGAGGTTGCGCCCCAGCATGTGCAGGGCACGCAGGCACTGGTAGAAACCCTGCTGCAGGCGTTCATCGACGAAGGGCCCACCCAGCTGGAGCTGCAACTGGCACGCAAACAGCTGGAAGGGCAGTTGCTGCGAGGGATCGCG  >  NC_002947/5838069‑5838360
                                                                                                                                                |                                                                                                                                                   
gTTTCTGGCCCTGGCCTTGGCAAGCGCTGTTCTGGGAGAGGGCCTCGAGTCGCGCTTGATGGTGGAGTTGCGCCAACGACGCGGCCTGACCTACGGCGTGCACACGCTTGTATTGCCATTGAGTGCAGGCGGCCTGTTCACCGTGGAAt                                                                                                                                                 <  8:173166/149‑1 (MQ=255)
gTTTCTGGCCCTGGCCTTGGCAAGCGCTGTTCTGGGAGAGGGCCTCGAGTCGCGCTTGATGGTGGAGTTGCGCCAACGACGCGGCCTGACCTACGGCGTGCACACGCATGTATTGCCATTGAGTGCAGGCGGCCTGTTCACCGTGGAAt                                                                                                                                                 <  6:77035/149‑1 (MQ=255)
       gccctggccTTGGCAAGCGCTGTTCTGGGAGAGGGCCTCGAGTCGCGCTTGATGGTGGAGTTGCGCCAACGACGCGGCCTGACCTACGGCGTGCACACGCATGTATTGCCATTGAGTGCAGGCGGCCTGTTCACCGTGGAATGGGAgg                                                                                                                                           <  1:166048/148‑1 (MQ=255)
        ccctggccTTGGCAAGCGCTGTTCTGGGAGAGGGCCTCGAGTCGCGCTTGATGGTGGAGTTGCGCCAACGACGCGGCCTGACCTACGGCGTGCACACGCATGTATTGCCATTGAGTGCAGGCGGCCTGTTCACCGTGGAATGGGAGGtt                                                                                                                                         >  4:288604/1‑149 (MQ=255)
               cTTGGCAAGCGCTGTTCTGGGAGAGGGCCTCGAGTCGCGCTTGATGGTGGAGTTGCGCCAACGACGCGGCCTGACCTACGGCGTGCACACGCATGTATTGCCATTGAGTGCAGGCGGCCTGTTCACCGTGGAATGGGAGGTTGCGccc                                                                                                                                   >  3:57392/1‑148 (MQ=255)
                  ggCAAGCGCTGTTCTGGGAGAGGGCCTCGAGTCGCGCTTGATGGTGGAGTTGCGCCAACGACGCGGCCTGACCTACGGCGTGCACACGCATGTATTGCCATTGAGTGCAGGCGGCCTGTTCACCGTGGAATGGTAGGTTGCGCCCCa                                                                                                                                 <  4:145806/147‑1 (MQ=255)
                          cTGTTCTGGGAGAGGGCCTCGAGTCGCGCTTGATGGTGGAGTTGCGCCAACGACGCGGCCTGACCTACGGCGTGCACACGCATGTATTGCCATTGAGTGCAGGCGGCCTGTTCACCGTGGAATGGGAGGTTGCGCCCCAACATGTGCAg                                                                                                                       <  4:57392/149‑1 (MQ=255)
                           tGTTCTGGTAGTGGGCTTCGTGTCTCGCGTGTTGGGTGATTTGCGACAACGACGCGGCCTGACCTACGGCGTGCTCACGCATGTATTGCCATTGAGTGCAGGCGGCCTGTTCACCGTGGAATGGGAGGTTGGGCCCCAGCATGTGCAgg                                                                                                                      <  6:366384/149‑1 (MQ=255)
                             ttCTGGGAGAGGGCCTCGAGTCGCGCTTGATGGTGGAGTTGCGCCAACGACGCGGCCTGACCTACGGCGTGCACACGCATGTATTGCCATTGAGTGCAGGCGGCCTGTTCACCGTGGAATGGGAGGTTGCGCCCCAGCATGTGCAggg                                                                                                                     >  1:1013/1‑148 (MQ=255)
                             ttCTGGGAGAGGGCCTCGAGTCGCGCTTGATGGTGGAGTTGCGCCAACGACGCGGCCTGACCTACGGCGTGCACACGCATGTATTGCCATTGAGTGCAGGCGGCCTGTTCACCGTGGAATGGGAGGTTGCGCCCCAGCATGTGCAgg                                                                                                                      <  4:102853/147‑1 (MQ=255)
                             ttCTGGGAGAGGGCCTCGAGTCGCGCTTGATGGTGGAGTTGCGCCAACGACGCGGCCTGACCTACGGCGTGCACACGCATGTATTGCCATTGAGTGCAGGCGGCCTGTTCACCGTGGAATGGGAGGTTGCGCCCCAGCATGTGCAgg                                                                                                                      <  1:55298/147‑1 (MQ=255)
                                tGGGAGAGGGCCTCGAGTCGCGCTTGATGGTGGAGTTGCGCCAACGACGCGGCCTGACCTACGGCGTGCACACGCATGTATTGCCATTGAGTGCAGGCGGCCTGTTCACCGTGGAATGGGAGGTTGCGCCCCAGCATGTGCAGGGCACg                                                                                                                 >  1:167434/1‑149 (MQ=255)
                                   gagaGGGCCTCGAGTCGCGCTTGATGGTGGAGTTGCGCCAACGACGCGGCCTGACCTACGGCGTGCACACGCATGTATTGCCATTGAGTGCAGGCGGCCTGTTCACCGTGGAATGGGa                                                                                                                                             >  2:323676/1‑118 (MQ=255)
                                   gagaGGGCCTCGAGTCGCGCTTGATGGTGGAGTTGCGCCAACGACGCGGCCTGACCTACGGCGTGCACACGCATGTATTGCCATTGAGTGCAGGCGGCCTGTTCACCGTGGAATGGGa                                                                                                                                             <  1:323676/118‑1 (MQ=255)
                                   gagaGGGCCTCGAGTCGCGCTTGATGGTGGAGTTGCGCCAACGACGCGGCCTGACCTACGGCGTGCACACGCATGTATTGCCATTGAGTGCAGGCGGCCTGTTCACCGTGGAATGGGAGGTTGCGCCCCAGCATGTGCAGGGCACGCAg                                                                                                              <  6:326991/149‑1 (MQ=255)
                                    agagGGCCTCGAGTCGCGCTTGATGGTGGAGTTGCGCCAACGACGCGGCCTGACCTACGGCGTGCACACGCATGTATTGCCATTGAGTGCAGGCGGCCTGTTCACCGTGGAATGGGAGGTTGCGCCCCAGCATGTGCAGGGCACGCAgg                                                                                                             >  2:195633/1‑149 (MQ=255)
                                          ccTCGAGTCGCGCTTGATGGTGGAGTTGCGCCAACGACGCGGCCTGACCTACGGCGTGCACACGCATGTATTGCCATTGAGTGCAGGCGGCCTGTTCACCGTGGAATGGGAGGTTGCGCCCCAGCATGTGCAgg                                                                                                                      <  4:302345/134‑1 (MQ=255)
                                          ccTCGAGTCGCGCTTGATGGTGGAGTTGCGCCAACGACGCGGCCTGACCTACGGCGTGCACACGCATGTATTGCCATTGAGTGCAGGCGGCCTGTTCACCGTGGAATGGGAGGTTGCGCCCCAGCATGTGCAgg                                                                                                                      >  3:302345/1‑134 (MQ=255)
                                          ccTCGAGTCGCGCTTGATGGTGGAGTTGCGCCAACGACGCGGCCTGACCTACGGCGTGCACACGCATGTATTGCCATTGAGTGCAGGCGGCCTGTTCACCGTGGAATGGGAGGTTGCGCCCCAGCATGTGCAGGGCACGCAGGCACTgg                                                                                                       <  3:288604/149‑1 (MQ=255)
                                          ccTCGAGTCGCGCTTGATGGTGGAGTTGCGCCAACGACGCGGCCTGACCTACGGCGTGCACACGCATGTATTGCCATTGAGTGCAGGCGGCCTGTTCACCGTGGAATGGGAGGTTGCGCCCCAGCATGTGCAGGGCACGCAGGCACTgg                                                                                                       >  7:325671/1‑149 (MQ=255)
                                              gAGTCGCGCTTGATGGTGGAGTTGCGCCAACGACGCGGCCTGACCTACGGCGTGCACACGCATGTATTGCCATTGAGTGCAGGCGGCCTGTTCACCGTGGAATGGGAGGTTGCGCCCCAGCATGTGCAGGGCACGCAGGCACTGGTa                                                                                                     <  2:167434/147‑1 (MQ=255)
                                              gAGTCGCGCTTGATGGTGGAGTTGCGCCAACGACGCGGCCTGACCTACGGCGTGCACACGCATGTATTGCCATTGAGTGCAGGCGGCCTGTTCACCGTGGAATGGGAGGTTGCGCCCCAGCATGTGCAGGGCACGCAGGCACTGGTAg                                                                                                    >  8:181820/1‑148 (MQ=255)
                                                gTCGCGCTTGATGGTGGAGTTGCGCCAACGACGCGGCCTGACCTACGGCGTGCACACGCATGTATTGCCATTGAGTGCAGGCGGCCTGTTCACCGTGGAATGGGAGGTTGCGCCCCAGCATGTGCAGGGCACGCAGGCACTGGTAGaaa                                                                                                 >  3:15888/1‑149 (MQ=255)
                                                gTCGCGCTTGATGGTGGAGTTGCGCCAACGACGCGGCCTGACCTACGGCGTGCACACGCATGTATTGCCATTGAGTGCAGGCGGCCTGTTCACCGTGGAATGGGAGGTTGCGCCCCAGCATGTGCAGGGCACGCAGGCACTGGTAGaaa                                                                                                 >  4:302002/1‑149 (MQ=255)
                                                   gcgcTTGATGGTGGAGTTGCGCCAACGACGCGGCCTGACCTACGGCGTGCACACGCATGTATTGCCATTGAGTGCAGGCGGCCTGTTCACCGTGGAATGGGAGGTTGCGCCCCAGCATGTGCAGGGCACGCAGGCACTGGTAGAAAccc                                                                                              <  7:181820/149‑1 (MQ=255)
                                                     gcTTGATGGTGGAGTTGCGCCAACGACGCGGCCTGACCTACGGCGTGCACACGCATGTATTGCCATTGAGTGCAGGCGGCCTGTTCACCGTGGAATGGGAGGTTGCGCCCCAGCATGTGCAGGGCACGCAGGCACTGGTAGAAACCctg                                                                                            <  3:302002/149‑1 (MQ=255)
                                                     gcTTGATGGTGGAGTTGCGCCAACGACGCGGCCTGACCTACGGCGTGCACACGCATGTATTGCCATTGAGTGCAGGCGGCCTGTTCACCGTGGAATGGGAGGTTGCGCCCCAGCATGTGCAGGGCACGCAGGCACTGGTAGAAACCctg                                                                                            <  3:128612/149‑1 (MQ=255)
                                                     gcTTGATGGTGGAGTTGCGCCAACGACGCGGCCTGACCTACGGCGTGCACACGCATGTATTGCCATTGAGTGCAGGCGGCCTGTTCACCGTGGAATGGGAGGTTGCGCCCCAGCATGTGCAGGGCACGCAGGCACTGGTAGAAACCctg                                                                                            <  2:1013/149‑1 (MQ=255)
                                                                gAGTTGCGCCAACGACGCGGCCTGACCTACGGCGTGCACACGCATGTATTGCCATTGAGTGCAGGCGGCCTGTTCACCGTGGAATGGGAGGTTGCGCCCCAGCATGTGCAGGGCACGCAGGCACTGGTAGAAACCCTGCTGCAGGCGt                                                                                  <  8:325671/148‑1 (MQ=255)
                                                                       gcCAACGACGCGGCCTGACCTACGGCGTGCACACGCATGTATTGCCATTGAGTGCAGGCGGCCTGTTCACCGTGGAATGGGAGGTTGCGCCCCAGCATGTGCAGGGCACGCAGGCACTGGTAGAAACCCTGCTGCAGGCGTTCATcgac                                                                          >  5:252596/1‑149 (MQ=255)
                                                                         cAACGACGCGGCCTGACCTACGGCGTGCACACGCATGTATTGCCATTGAGTGCAGGCGGCCTGTTCACCGTGGAATGGGAGGTTGCGCCCCAGCATGTGCAGGGCACGCAGGc                                                                                                            >  8:208190/1‑113 (MQ=255)
                                                                         cAACGACGCGGCCTGACCTACGGCGTGCACACGCATGTATTGCCATTGAGTGCAGGCGGCCTGTTCACCGTGGAATGGGAGGTTGCGCCCCAGCATGTGCAGGGCACGCAGGc                                                                                                            <  7:208190/113‑1 (MQ=255)
                                                                         cAACGACGCGGCCTGACCTACGGCGTGCACACGCATGTATTGCCATTGAGTGCAGGCGGCCTGTTCACCGTGGAATGGGAGGTTGCGCCCCAGCATGTGCAGGGCACGCAGGCACTGGTAGAAAc                                                                                                <  8:15817/125‑1 (MQ=255)
                                                                         cAACGACGCGGCCTGACCTACGGCGTGCACACGCATGTATTGCCATTGAGTGCAGGCGGCCTGTTCACCGTGGAATGGGAGGTTGCGCCCCAGCATGTGCAGGGCACGCAGGCACTGGTAGAAAc                                                                                                >  7:15817/1‑125 (MQ=255)
                                                                                         ccTACGGCGTGCACACGCATGTATTGCCATTGAGTGCAGGCGGCCTGTTCACCGTGGAATGGGAGGTTGCGCCCCAGCATGTGCAGGGCACGCAGGCACTGGTAGAAACCCTGCTGCAGGCGTTCATCGACGAAGGGCCCACCCAGCTg                                                        <  4:15888/149‑1 (MQ=255)
                                                                                           tACGGCGTGCACACGCATGTATTGCCATTGAGTGCAGGCGGCCTGTTCACCGTGGAATGGGAGGTTGCGCCCCAGCATGTGCAGGGCACGCAGGCACTGGTAGAAACCCTGCTGCAGGCGTTCATCGACGAAGGGCCCACCCAGCTg                                                        >  5:55549/1‑147 (MQ=255)
                                                                                                 gTGCACACGCATGTATTGCCATTGAGTGCAGGCGGCCTGTTCACCGTGGAATGGGAGGTTGCGCCCCAGCATGTGCAGGGCACGCAGGCACTGGTAGAAACCCTGCTGCAGGCGTTCATCGACGAAGGGCCCACCCAGCTGGAGCTGc                                                 >  2:265932/1‑148 (MQ=255)
                                                                                                      cacGCATGTATTGCCATTGAGTGCAGGCGGCCTGTTCACCGTGGAATGGGAGGTTGCGCCCCAGCATGTTCAGGGCACGCAGGCACTGGTAGAAACCCTGCTGCAGGCGTTCATCGACGAAGGGCCCACCCAGCTGGAGCTGCAACTgg                                           <  3:271664/149‑1 (MQ=255)
                                                                                                       acGCATGTATTGCCATTGAGTGCAGGCGGCCTGTTCACCGTGGAATGGGAGGTTGCGCCCCAGCATGTGCAGGGCACGCAGGCACTGGTAGAAACCCTGCTGCAGGCGTTCATCGACGAAGGGCCCACCCAGCTGGAGCTGCAACTgg                                           >  6:353959/1‑148 (MQ=255)
                                                                                                         gCATGTATTGCCATTGAGTGCAGGCGGCCTGTTCACCGTGGAATggg                                                                                                                                              <  5:250127/47‑1 (MQ=255)
                                                                                                         gCATGTATTGCCATTGAGTGCAGGCGGCCTGTTCACCGTGGAATggg                                                                                                                                              >  6:250127/1‑47 (MQ=255)
                                                                                                         gCATGTATTGCCATTGAGTGCAGGCGGCCTGTTCACCGTGGAATGGGAGGTTGCGCCCCAGCATGTGCAGGGCACGCAGGCACTGGTAGAAACCCTGCTGCAGGCGTTCATCGACGAAGGGCCCACCCAGCTGGAGCTGCAACTGGCAc                                        >  8:285905/1‑149 (MQ=255)
                                                                                                              tATTGCCATTGAGTGCAGGCGGCCTGTTCACCGTGGAATGGGAGGTTGCGCCCCAGCATGTGCAGGGCACGCAGGCACTGGTAGAAACCCTGCTGCAGGCGTTCATCGACGAAGGGCCCACCCAGCTGGAGCTGCAACTGGCACGCaaa                                   >  4:125663/1‑149 (MQ=255)
                                                                                                               aTTGCCATTGAGTGCAGGCGGCCTGTTCACCGTGGAATGGGAGGTTGCGCCCCAGCATGTGCAGGGCACGCAGGCACTGGTAGAAACCCTGCTGCAGGCGTTCATCGACGAAGGGCCCACCCAGCTGGAGCTGCAACTGGCACGCaaa                                   >  7:260418/1‑148 (MQ=255)
                                                                                                                ttGCCATTGAGTGCAGGCGGCCTGTTCACCGTGGAATGGGAGGTTGCGCCCCAGCATGTGCAGGGCACGCAGGCACTGGTAGAAACCCTGCTGCAGGCGTTCATCGACGAAGGGCCCACCCAGCTGGAGCTGCAACTGGCACGCAAACa                                 <  1:195633/149‑1 (MQ=255)
                                                                                                                    gaTTGAGTGCAGGCGGCCTGTTCACCGTGGAATGGGAGGTTGCGCCCCAGCATGTGCAGGGCACGCAGGCACTGGTAGAAACCCTGCTGCAGGCGTTCATCGACGAAGGGCCCACCCAGCTGGAGCTGCAACTGGCACGCAAACAGCTg                             <  6:252596/148‑1 (MQ=255)
                                                                                                                     aTTGAGTGCAGGCGGCCTGTTCACCGTGGAATGGGAGGTTGCGCCCCAGCATGTGCAGGGCACGCAGGCACTGGTAGAAACCCTGCTGCAGGCGTTCATCGACGAAGGGCCCACCCAGCTGGAGCTGCAACTGGCACGCAAACAGCTg                             >  3:221902/1‑148 (MQ=255)
                                                                                                                        gAGTGCAGGCGGCCTGTTCACCGTGGAATGGGAGGTTGCGCCCCAGCATGTGCAGGGCACGCAGGCACTGGTAGAAACCCTGCTGCAGGCGTTCATCGACGAAGGGCCCACCCAGCTGGAGCTGCAACTGGCACGCAAACAGCTGGaa                          <  5:353959/148‑1 (MQ=255)
                                                                                                                              aGGCGGCCTGTTCACCGTGGAATGGGAGGTTGCGCCCCAGCATGTGCAGGGCACGCAGGCACTGGTAGAAACCCTGCTGCAGGCGTTCATCGACGAAGGGCCCACCCAGCTGGAGCTGCAACt                                             >  7:322931/1‑123 (MQ=255)
                                                                                                                              aGGCGGCCTGTTCACCGTGGAATGGGAGGTTGCGCCCCAGCATGTGCAGGGCACGCAGGCACTGGTAGAAACCCTGCTGCAGGCGTTCATCGACGAAGGGCCCACCCAGCTGGAGCTGCAACt                                             <  8:322931/123‑1 (MQ=255)
                                                                                                                                gcggcCTGTTCACCGTGGAATGGGAGGTTGCGCCCCAGCATGTGCAGGGCACGCAGGCACTGGTAGAAACCCTGCTGCAGGCGTTCATCGACGAAGGGCCCACCCAGCTGGAGCTGCAACTGGCACGCAAACAGCTGGAAGGGCAGTtg                 >  2:168522/1‑149 (MQ=255)
                                                                                                                                   gcCTGTTCACCGTGGAATGGGAGGTTGCGCCCCAGCATGTGCAGGGCACGCAGGCACTGGTAGAAACCCTGCTGCAGGCGTTCATCGACGAAGGGCCCACCCAGCTGGAGCTGCAACTGGCACGCAAACAGCTGGAAGGGCAGTtgctg              <  1:265932/149‑1 (MQ=255)
                                                                                                                                        ttCACCGTGGAATGGGAGGTTGCGCCCCAGCATGTGCAGGGCACGCAGGCACTGGTAGAAACCCTGCTGCAGGCGTTCATCGACGAAGGGCCCACCCAGCTGGAGCTGCAACTGGCACGCAAACAGCTGGAAGGGCAGTTGCTGCGAg          <  3:125663/148‑1 (MQ=255)
                                                                                                                                        ttCACCGTGGAATGGGAGGTTGCGCCCCAGCATGTGCAGGGCACGCAGGCACTGGTAGAAACCCTGCTGCAGGCGTTCATCGACGAAGGGCCCACCCAGCTGGAGCTGCAACTGGCACGCAAACAGCTGGAAGGGCAGTTGCTGCGAg          <  6:55549/148‑1 (MQ=255)
                                                                                                                                        ttCACCGTAGAATGGGAGGTTGCGCCCCAGCATGTGCAGGGCACGCAGGCACTGGTAGAAACCCTGCTGCAGGCGTTCATCGACGAAGGGCCCACCCAGCTGGa                                                      <  7:309389/104‑1 (MQ=255)
                                                                                                                                        ttCACCGTAGAATGGGAGGTTGCGCCCCAGCATGTGCAGGGCACGCAGGCACTGGTAGAAACCCTGCTGCAGGCGTTCATCGACGAAGGGCCCACCCAGCTGGa                                                      <  7:314588/104‑1 (MQ=255)
                                                                                                                                        ttCACCGTAGAATGGGAGGTTGCGCCCCAGCATGTGCAGGGCACGCAGGCACTGGTAGAAACCCTGCTGCAGGCGTTCATCGACGAAGGGCCCACCCAGCTGGa                                                      >  8:309389/1‑104 (MQ=255)
                                                                                                                                        ttCACCGTAGAATGGGAGGTTGCGCCCCAGCATGTGCAGGGCACGCAGGCACTGGTAGAAACCCTGCTGCAGGCGTTCATCGACGAAGGGCCCACCCAGCTGGa                                                      >  8:314588/1‑104 (MQ=255)
                                                                                                                                               tGGAATGGGAGGTTGCGCCCCAGCATGTGCAGGGCACGCAGGCACTGGTAGAAACCCTGCTGCAGGCGTTCATCGACGAAGGGCCCACCCAGCTGGAGCTGCAACTGGCACGCAAACAGCTGGAAGGGCAGTTGCTGCGAGGGATcgcg  <  8:260418/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
GTTTCTGGCCCTGGCCTTGGCAAGCGCTGTTCTGGGAGAGGGCCTCGAGTCGCGCTTGATGGTGGAGTTGCGCCAACGACGCGGCCTGACCTACGGCGTGCACACGCATGTATTGCCATTGAGTGCAGGCGGCCTGTTCACCGTGGAATGGGAGGTTGCGCCCCAGCATGTGCAGGGCACGCAGGCACTGGTAGAAACCCTGCTGCAGGCGTTCATCGACGAAGGGCCCACCCAGCTGGAGCTGCAACTGGCACGCAAACAGCTGGAAGGGCAGTTGCTGCGAGGGATCGCG  >  NC_002947/5838069‑5838360

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑