Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,893,031	G→C	6.8%	A80G (GCA→GGA)	PP_5167 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,893,031	0	G	C	6.8%	191.4 / 3.9	59	A80G (GCA→GGA)	PP_5167	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (28/27);  new base C (2/2);  total (30/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

ACAACCTGGCTAGGGAACGGCAGCACCTCGGTAGGGATCGGCGCAAAGTTGCGCAGCACGGGTGCGCAAGTGGGCCGTTCGACATCTGCCGGCGCCACCAGCAGTGCACCACGCACCTGCCGTAGC‑AAGGCCGGGCTTGCCTGCGCTGCCCAATGGGCGACAGTGATGCAACCAAGGCTGTGGGCAATCAGGATCACCGGCGAGCGCTCGGCGGCAATCGCCTGCTCCAACGCGTGCACCCAGTCCTGCCGCTGCGGGGTGAGCCAGTCTTGCTGTTCAACCCGTGCACTGTTG  >  NC_002947/5892884‑5893177                                                                                                                                                     |                                                                                                                                                   aCAACCTGGCTAGGGAACGGCAGCACCTCGGTAGGGATCGGCGCAAAGTTGCGCAGCACGGGTGCGCAAGTGGGCCGTTCGACATCTGCCGGCGCCACCAGCAGTGCACCACGCACCTGCCGTAGC‑AAGGCCGGGCTTGCCTGCGCTGc                                                                                                                                                   >  3:264718/1‑149 (MQ=255)  cAACCTGGCTAGGGAACGGCAGCACCTCGGTAGGGATCGGCGCAAAGTTGCGCAGCACGGGTGCGCAAGTGGGCCGTTCGACATCTGCCGGCGCCACCAGCAGTGCACCACGCACCTGCCGTAGC‑AAGGCCGGGCTTGCCTGCGCTGcc                                                                                                                                                  <  5:68877/149‑1 (MQ=255)   aaCCTGGCTAGGGAACGGCAGCACCTCGGTAGGGATCGGCGCAAAGTTGCGCAGCACGGGTGCGCAAGTGGGCCGTTCGACATCTGCCGGCGCCACCAGCAGTGCACCACGCACCTGCCGTAGC‑AAGGCCGGGCTTGCCTGCGCTGcc                                                                                                                                                  >  2:101726/1‑148 (MQ=255)          cTAGGGAACGGCAGCACCTCGGTAGGGATCGGCGCAAAGTTGCGCAGCACGGGTGCGCAAGTGGGCCGTTCGACATCTGCCGGCGCCACCAGCAGTGCACCACGCACCTGCCGTAGC‑AAGGCCGGGCTTGCCTGCGCTGCCCAATGGGc                                                                                                                                          <  4:79619/149‑1 (MQ=255)           tAGGGAACGGCAGCACCTCGGTAGGGATCGGCGCAAAGTTGCGCAGCACGGGTGCGCAAGTGGGCCGTTCGACATCTGCCGGCGCCACCAGCAGTGCACCACGCACCTGCCGTAGC‑AAGGCCGGGCTTGCCTGCGCTGCCCAATGGGc                                                                                                                                          >  7:71447/1‑148 (MQ=255)              ggAACGGCAGCACCTCGGTAGGGATCGGCGCAAAGTTGCGCAGCACGGGTGCGCAAGTGGGCCGTTCGACATCTGCCGGCGCCACCAGCAGTGCACCACGCACCTGCCGTAGC‑AAGGCCGGGCTTGCCTGCGCTGCCCAATGGGCGa                                                                                                                                        >  3:321689/1‑147 (MQ=255)                  cGGCAGCACCTCGGTAGGGATCGGCGCAAAGTTGCGCAGCACGGGTGCGCAAGTGGGCCGTTCGACATCTGCCGGCGCCACCAGCAGTGCACCACGCACCTGCCGTAGC‑AAGGCCGGGCTTGCCTGCGCTGCCCAATGGGCGACAGTGa                                                                                                                                  <  4:146160/149‑1 (MQ=255)                     cagcaCCTCGGTAGGGATCGGCGCAAAGTTGCGCAGCACGGGTGCGCAAGTGGGCCGTTCGACATCTGCCGGCGCCACCAGCAGTGCACCACGCACCTGCCGTAGC‑AAGGCCGGGCTTGCCTGCGCTGCCCAATGGGCGACAGTGATGc                                                                                                                               <  7:267049/149‑1 (MQ=255)                          ccTCGGTAGGGATCGGCGCAAAGTTGCGCAGCACGGGTGCGCAAGTGGGCCGTTCGACATCTGCCGGCGCCACCAGCAGTGCACCACGCACCTGCCGTAGC‑AAGGCCGGGCTTGCCTGCGCTGCCCAATGGGCGACAGTGATGCAAcc                                                                                                                           >  3:93528/1‑148 (MQ=255)                          ccTCGGTAGGGATCGGCGCAAAGTTGCGCAGCACGGGTGCGCAAGTGGGCCGTTCGACATCTGCCGGCGCCACCAGCAGTGCACCACGCACCTGCCGTAGC‑AAGGCCGGGCTTGCCTGCGCTGCCCAATGGGCGACAGTGATGCAAcc                                                                                                                           >  6:27229/1‑148 (MQ=255)                           cTCGGTAGGGATCGGCGCAAAGTTGCGCAGCACGGGTGCGCAAGTGGGCCGTTCGACATCTGCCGGCGCCACCAGCAGTGCACCACGCACCTGCCGTAGC‑AAGGCCGGGCTTGCCTGCGCTGCCCAATGGGCGACAGTGATGCAACCaa                                                                                                                         <  4:321689/149‑1 (MQ=255)                                   ggATCGGCGCAAAGTTGCGCAGCACGGGTGCGCAAGTGGGCCGTTCGACATCTGCCGGCGCCACCAGCAGTGCACCACGCACCTGCCGTAGC‑AAGGCCGGGCTTGCCTGCGCTGCCCAATGGGCGACAGTGATGCTACCAAGGCTGTgg                                                                                                                 <  8:30854/149‑1 (MQ=255)                                   ggATCGGCGCAAAGTTGCGCAGCACGGGTGCGCAAGTGGGCCGTTCGACATCTGCCGGCGCCACCAGCAGTGCACCACGCACCTGCCGTAGC‑AAGGCCGGGCTTGCCTGCGCTGCCCAATGGGCGACAGTGATGCAACCAAGGCtgtg                                                                                                                  <  4:264718/148‑1 (MQ=255)                                   ggATCGGCGCAAAGTTGCGCAGCACGGGTGCGCAAGTGGGCCGTTCGACATCTGCCGGCGCCACCAGCAGTGCACCACGCACCTGCCGTAGC‑AAGGCCGGGCTTGCCTGCGCTGCCCAATGGGCGACAGTCATGCAACCAAGGCTGTgg                                                                                                                 >  1:273830/1‑149 (MQ=255)                                    gATCGGCGCAAAGTTGCGCAGCACGGGTGCGCAAGTGGGCCGTTCGACATCTGCCGGCGCCACCAGCAGTGCACCACGCACCTGCCGTAGCTCAGGCCGGGCTTGCCTGCGCTGCCCAATGGGCGACAGTGATGCAACCAAGGCTGTgg                                                                                                                 <  1:101726/149‑1 (MQ=255)                                          cgcAAAGTTGCGCAGCACGGGTGCGCAAGTGGGCCGTTCGACATCTGCCGGCGCCACCAGCAGTGCACCACGCACCTGCCGTAGC‑AAGGCCGGGCTTGCCTGCGCTGCCCAATGGGCGACAGTGATGCAACCAAGGCTGTGGGCAAt                                                                                                            >  8:161537/1‑147 (MQ=255)                                           gcAAAGTTGCGCAGCACGGGTGCGCAAGTGGGCCGTTCGACATCTGCCGGCGCCACCAGCAGTGCACCACGCACCTGCCGTAGC‑AAGGCCGGGCTTGCCTGCGCTGCCCAATGGGCGACAGTGATGCAACCAAGGCTGTGGGCAATCAg                                                                                                         >  3:260170/1‑149 (MQ=255)                                                     gcagcaCGGGTGCGCAAGTGGGCCGTTCGACATCTGCCGGCGCCACCAGCAGTGCACCACGCACCTGCCGTAGC‑AAGGCCGGGCTTGCCTGCGCTGCCCAATGGGCGACAGTGATGCAACCAAGGCTGTGGGCAATCAGGATCACCGGc                                                                                               <  5:27229/149‑1 (MQ=255)                                                     gcagcaCGGGTGCGCAAGTGGGCCGTTCGACATCTGCCGGCGCCACCAGCAGTGCACCACGCACCTGCCGTAGC‑AAGGCCGGGCTTGCCTGCGCTGCCCAATGGGCGACAGTGATGCAACCAAGGCTGTGGGCAATCAGGATCACCGGc                                                                                               <  8:71447/149‑1 (MQ=255)                                                       agcaCGGGTGCGCAAGTGGGCCGTTCGACATCTGCCGGCGCCACCAGCAGTGCACCACGCACCTGCCGTAGC‑AAGGCCGGGCTTGCCTGCGCTGCCCAATGGGCGACAGTGATGCAACCAAGGCTGTGGGCAATCAGGATCACCGGCGa                                                                                             <  7:68968/149‑1 (MQ=255)                                                              ctGCGCAAGTGGGCCGTTCGACATCTGCCGGCGCCACCAGCAGTGCACCACGCACCTGCCGTAGC‑AAGGCCGGGCTTGCCTGCGCTGCCCAATGGGCGACAGTGATGCAACCAAGGCTGTGGGCAATCAGGATCACCGGCGAGCGCTc                                                                                       <  4:301980/147‑1 (MQ=255)                                                                  gcAAGTGGGCCGTTCGACATCTGCCGGCGCCACCAGCAGTGCACCACGCACCTGCCGTAGC‑AAGGCCGGGCTTGCCTGCGCTCCCCAATGGGCGACAGTGATGCAACCAAGGCTGTGGGCAATCAGGATCACCGGCGAGCGCTcggcgg                                                                                  >  1:142261/1‑149 (MQ=255)                                                                  gcAAGTGGGCCGTTCGACATCTGCCGGCGCCACCAGCAGTGCACCACGCACCTGCCGTAGC‑AAGGCCGGGCTTGCCTGCGCTCCCCAATGGGCGACAGTGATGCAACCAAGGCTGTGGGCAATCAGGATCACCGGCGAGCGCTcggcgg                                                                                  >  1:136742/1‑149 (MQ=255)                                                                    aaGTGGGCCGTTCGACATCTGCCGGCGCCACCAGCAGTGCACCACGCACCTGCCGTAGC‑AAGGCCGGGCTTGCCTGCGCTGCCCAATGGGCGACAGt                                                                                                                                    <  2:330721/97‑1 (MQ=255)                                                                    aaGTGGGCCGTTCGACATCTGCCGGCGCCACCAGCAGTGCACCACGCACCTGCCGTAGC‑AAGGCCGGGCTTGCCTGCGCTGCCCAATGGGCGACAGt                                                                                                                                    >  1:330721/1‑97 (MQ=255)                                                                         ggCCGTTCGACATCTGCCGGCGCCACCAGCAGTGCACCACGCACCTGCCGTAGC‑AAGGCCGGGCTTGCCTGCGCTGCCCAATGGGCGACAGTGATGCAACCAAGGCTGTGGGCAATCAGGATCACCGGCGAGCGCTCGGCGGCAATCGc                                                                           <  7:161537/149‑1 (MQ=255)                                                                           ccGTTCGACATCTGCCGGCGCCACCAGCAGTGCACCACGCACCTGCCGTAGC‑AAGGCCGGGCTTGCCTGCGCTGCCCAATGGGCGACAGTGATGCAACCAAGGCTGTGGGCAATCAGGATCACCGGCGAGCGCTCGGCGGCAATCGCCt                                                                         <  4:93528/149‑1 (MQ=255)                                                                            cGTTCGACATCTGCCGGCGCCACCAGCAGTGCACCACGCACCTGCCGTAGC‑AAGGCCGGGCTTGCCTGCGCTGCCCAATGGGCGACAGTGATGCAACCAAGGCTGTGGGCAATCAGGATCAc                                                                                                   <  3:80244/122‑1 (MQ=255)                                                                            cGTTCGACATCTGCCGGCGCCACCAGCAGTGCACCACGCACCTGCCGTAGC‑AAGGCCGGGCTTGCCTGCGCTGCCCAATGGGCGACAGTGATGCAACCAAGGCTGTGGGCAATCAGGATCAc                                                                                                   >  4:80244/1‑122 (MQ=255)                                                                                 gACATCTGCCGGCGCCACCAGCAGTGCACCACGCACCTGCCGTAGC‑AAGGCCGGGCTTGCCTGCGCTGCCCAATGGGCGACAGTGATGCAACCAAGGCTGTGGGCAATCAGGATCACCGGCGAGCGCTCGGCGGCAATCGCCTGCTcc                                                                    <  8:44855/148‑1 (MQ=255)                                                                                    aTCTGCCGGCGCCACCAGCAGTGCACCACGCACCTGCCGTAGC‑AAGGCCGGGCTTGCCTGCGCTGCCCAATGGGCGACAGTGATGCAACCAAGGCTGTGGGCAATCAGGATCACCGGCGAGCGCTCGGCGGCAATCGCCTGCTCCAAcg                                                                >  3:334749/1‑149 (MQ=255)                                                                                         ccGGCGCCACCAGCAGTGCACCACGCACCTGCCGTAGC‑AAGGCCGGGCTTGCCTGCGCTGCCCAATGGGCGACAGTGATGCAACCAAGGCTGTGGGCAATCAGGATCACCGGCGAGCGCTCGGCGGCAATCGCCTGCTCCAACGCGTGc                                                           <  4:334749/149‑1 (MQ=255)                                                                                         ccGGCGCCACCAGCAGTGCACCACGCACCTGCCGTAGC‑AAGGCCGGGCTTGCCTGCGCTGCCCAATGGGCGACAGTGATGCAACCAAGGCTGTGGGCAATCAGGATCACCGGCGAGCGCTCGGCGGCAATCGCCTGCTCCAACGCGTGc                                                           <  1:41465/149‑1 (MQ=255)                                                                                             cgcCACCAGCAGTGCACCACGCACCTGCCGTAGC‑AAGGCCGGGCTTGCCTGCGCTGCCCAATGGGCGACAGTGATGCAACCAAGGCTGTGGGCAATCAGGATCACCGGCGAGCGCTCGGCGGCAATCGCCTGCTCCAACGCGTGCAcc                                                        >  2:337691/1‑148 (MQ=255)                                                                                               ccaccaGCAGTGCACCACGCACCTGCCGTAGC‑AAGGCCGGGCTTGCCTGCGCTGCCCAATGGGCGACAGTGATGCAACCAAGGCTGTGGGCAATCAGGATCACCGGCGAGCGCTCGGCGGCAATCGCCTGCt                                                                      >  7:327268/1‑132 (MQ=255)                                                                                               ccaccaGCAGTGCACCACGCACCTGCCGTAGC‑AAGGCCGGGCTTGCCTGCGCTGCCCAATGGGCGACAGTGATGCAACCAAGGCTGTGGGCAATCAGGATCACCGGCGAGCGCTCGGCGGCAATCGCCTGCt                                                                      <  8:327268/132‑1 (MQ=255)                                                                                                caccaGCAGTGCACCACGCACCTGCCGTAGC‑AAGGCCGGGCTTGCCTGCGCTGCCCAATGGGCGACAGTGATGCAACCAAGGCTGTGGGCAATCAGGATCACCGGCGAGCGCTCGGCGGCAATCGCCTGCTCCAACGCGTGCACCCAGt                                                    >  7:9809/1‑149 (MQ=255)                                                                                                 accaGCAGTGCACCACGCACCTGCCGTAGC‑AAGGCCGGGCTTGCCTGCGCTGCCCAATGGGCGACAGTGATGCAACCAAGGCTGTGGGCAATCAGGATCACCGGCGAGCGCTCGGCGGCAATCGCCTGCTCCAACGCGTGCACCCAGTc                                                   >  5:137456/1‑149 (MQ=255)                                                                                                      cagTGCACCACGCACCTGCCGTAGG‑AAGGCCGGGCTTGCCTGCGCTGCCCAATGGGCGACAGTGATGCAACCAAGGCTGTGGGCAATCAGGATCACCGGCGAGCGCTCGGCGGCAATCGCCTGCTCCAACGCGTGCACCCAGTCCTGcc                                              <  6:263118/149‑1 (MQ=255)                                                                                                            accacGCACCTGCCGTAGC‑AAGGCCGGGCTTGCCTGCGCTGCCCAATGGGCGACAGTGATGCAACCAAGGCTGTGGGCAATCAGGATCACCGGCGAGCGCTCGGCGGCAATCGCCTGCTCCAACGCGTGCACCCAGTCCTGCCGCTGCg                                        >  1:104589/1‑149 (MQ=255)                                                                                                              cacGCACCTGCCGTAGC‑AAGGCCGGGCTTGCCTGCGCTGCCCAATGGGCGACAGTGATGCAACCAAGGCTGTGGGCAATCAGGATCACCGGCGAGCGCTCGGCGGCAATCGCCTGCTCCAACGCGTGCACCCAGTCCTGCCGCTGCggg                                      >  7:328385/1‑149 (MQ=255)                                                                                                               acGCACCTGCCGTAGC‑AAGGCCGGGCTTGCCTGCGCTGCCCAATGGGCGACAGTGATGCAACCAAGGCTGTGGGCAATCAGGATCACCGGCGAGCGCTCGGCGGCAATCGCCTGCTCCAACGCGTGCACCCAGTCCTGCCGCTGCgggg                                     >  1:340890/1‑149 (MQ=255)                                                                                                                    ccTGCCGTAGC‑AAGGCCGGGCTTGCCTGCGCTGCCCAATGGGCGACAGTGATGCAACCAAGGCTGTGGGCAATCAGGATCACCGGCGAGCGCTCGGCGGCAATCGCCTGCTCCAACGCGTGCACCCAGTCCTGCCGCTGCGGGGTGAGc                                >  7:283087/1‑149 (MQ=255)                                                                                                                     cTGCCGTAGC‑AAGGCCGGGCTTGCCTGCGCTGCCCAATGGGCGACAGTGATGCAACCAAGGCTGTGGGCAATCAGGATCACCGGCGAGCGCTCGGCGGCAATCGCCTGCTCCAACGCGTGCACCCAGTCCTGCCGCTGCGGGGTGAGc                                >  6:240257/1‑148 (MQ=255)                                                                                                                              c‑AAGGCCGGGCTTGCCTGCGCTCCCCAATGGGCGACAGTGATGCAACCAAGGCTGTGGGCAATCAGGATCACCGGCGAGCGCTCGGCGGCAATCGCCTGCTCCAACGCGTGCACCCAGTCCTGCCGCTGCGGGGTGAGCCAGTCTTGCt                      <  2:142261/149‑1 (MQ=255)                                                                                                                                aaGGCCGGGCTTGCCTGCGCTCCCCAATGGGCGACAGTGATGCAACCAAGGCTGTGGGCAATCAGGATCACCGGCGAGCGCTCGGCGGCAATCGCCTGCTCCAACGCGTGCACCCAGTCCTGCCGCTGCGGGGTGAGCCAGTCTTGCt                      <  2:136742/148‑1 (MQ=255)                                                                                                                                  ggCCGGGCTTGCCTGCGCTGCCCAATGGGCGACAGTGATGCAACCAAGGCTGTGGGCAATCAGGATCACCGGCGAGCGCTCGGCGGCAATCGCCTGCTCCAACGCGTGCACCCAGTCCTGCCGCTGCGGGGTGAGCCAGTCTTGCTGtt                   <  8:9809/149‑1 (MQ=255)                                                                                                                                  ggCCGGGCTTGCCTGCGCTGCCCAATGGGCGACAGTGATGCAACCAAGGCTGTGGGCAATCAGGATCACCGGCGAGCGCTCGGCGGCAATCGCCTGCTCCAACGCGTGCACCCAGTCCTGCCGCTGCGGGGTGAGCCAGTCTTACTGt                    >  1:205156/1‑148 (MQ=255)                                                                                                                                   gCCGGGCTTGCCTGCGCTGCCCAATGGGCGACAGTGATGCAACCAAGGCTGTGGGCAATCAGGATCACCGGCGAGCGCTCGGCGGCAATCGCCTGCTCCAACGCGTGCACCCAGTCCTGCCGCTGCGGGGTGAGCCAGTCTTGCTGtt                   >  2:235246/1‑148 (MQ=255)                                                                                                                                    ccGGGCTTGCCTGCGCTGCCCAATGGGCGACAGTGATGCAACCAAGGCTGTGGGCAATCAGGATCACCGGCGAGCGCTCGGCGGCAATCGCCTGCTCCAACGCGTGCACCCAGTCCTGCCGCTGCggg                                      <  7:237807/128‑1 (MQ=255)                                                                                                                                    ccGGGCTTGCCTGCGCTGCCCAATGGGCGACAGTGATGCAACCAAGGCTGTGGGCAATCAGGATCACCGGCGAGCGCTCGGCGGCAATCGCCTGCTCCAACGCGTGCACCCAGTCCTGCCGCTGCggg                                      >  8:237807/1‑128 (MQ=255)                                                                                                                                    ccGGGCTTGCCTGCGCTGCCCAATGGGCGACAGTGATGCAACCAAGGCTGTGGGCAATCAGGATCACCGGCGAGCGCTCGGCGGCAATCGCCTGCTCCAACGCGTGCACCCAGTCCTGCCGCTGCGGGGTGAGCCAGTCTTGCTGTTc                  >  1:339675/1‑148 (MQ=255)                                                                                                                                          ttGCCTGCGCTGCCCAATGGGCGACAGTGATGCAACCAAGGCTGTGGGCAATCAGGATCACCGGCGAGCGCTCGGCGGCAATCGCCTGCTCCAACGCGTGCACCCAGTCCTGCCGCTGCGGGGTGAGCCAGTCTTGCTGtt                   >  2:43462/1‑141 (MQ=255)                                                                                                                                          ttGCCTGCGCTGCCCAATGGGCGACAGTGATGCAACCAAGGCTGTGGGCAATCAGGATCACCGGCGAGCGCTCGGCGGCAATCGCCTGCTCCAACGCGTGCACCCAGTCCTGCCGCTGCGGGGTGAGCCAGTCTTGCTGtt                   <  1:43462/141‑1 (MQ=255)                                                                                                                                          ttGCCTGCGCTGCCCAATGGGCGACAGTGATGCAACCAAGGCTGTGGGCAATCAGGATCACCGGCGAGCGCTCGGCGGCAATCGCCTGCTCCAACGCGTGCACCCAGTCCTGCCGCTGCGGGGTGAGCCAGTCTTGCTGTTCAACCCGt           <  2:300315/149‑1 (MQ=255)                                                                                                                                            gCCTGCGCTGCCCAATGGGCGACAGAGATGCAACCAAGCCTGTGGGCAATCAGGATCCCCGGCGCGCGCTCGGCGGCAATCGCCTGCTCCAACGCGTGCACCCAGTCCTGCCGCTGCGGTCTGAGCCAGTCTTGCTCTTCAACCCGTg          >  3:205107/1‑148 (MQ=255)                                                                                                                                              cTGCGCTGCCCAATGGGCGACAGTGATGCAACAAAGGCTGTGGGCAATCAGGATCACCGGCGAGCGCTCGGCGGCAATCGCCTGCTCCAACGCGTGCACCCAGTCCTGCCGCTGCGGGGTGAGCCCGTCTTGCGGTTCAACCCGTGccc       >  7:313035/1‑147 (MQ=255)                                                                                                                                                  gcTGCCCAATGGGCGACAGTGATGCAACCAAGGCTGTGGGCAATCAGGATCACCGGCGAGCGCTCGGCGGCAATCGCCTGCTCCAACGCGTGCACCCAGTCCTGCCGCTGCGGGGTGAGCCAGTCTTGCTGTTCAACCCGTGCACTGtt   <  2:339675/149‑1 (MQ=255)                                                                                                                                                   cTGCCCAATGGGCGACAGTCATGCAACCAAGGCTGTGGGCAATCACGATCACCGGCGAGCGCTCGGCGGCAATCGCCTGCTCCAACGCGTGCACCCAGTCCTGCCGCTGCGGGGTGAGCCAGTCTTGCTGTTCAACCCGTGCACTGTTg  <  2:273830/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                   ACAACCTGGCTAGGGAACGGCAGCACCTCGGTAGGGATCGGCGCAAAGTTGCGCAGCACGGGTGCGCAAGTGGGCCGTTCGACATCTGCCGGCGCCACCAGCAGTGCACCACGCACCTGCCGTAGC‑AAGGCCGGGCTTGCCTGCGCTGCCCAATGGGCGACAGTGATGCAACCAAGGCTGTGGGCAATCAGGATCACCGGCGAGCGCTCGGCGGCAATCGCCTGCTCCAACGCGTGCACCCAGTCCTGCCGCTGCGGGGTGAGCCAGTCTTGCTGTTCAACCCGTGCACTGTTG  >  NC_002947/5892884‑5893177

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑