Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,897,783 C→G 16.8% intergenic (‑183/+51) PP_5172 ← / ← PP_5173 hypothetical protein/RND family transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,897,7830CG16.8% 92.5 / 8.6 41intergenic (‑183/+51)PP_5172/PP_5173hypothetical protein/RND family transporter
Reads supporting (aligned to +/- strand):  ref base C (17/16);  new base G (4/3);  total (22/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAGGGGTTTTGCGCCTGGCTTGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAA‑CCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATC  >  NC_002947/5897641‑5897924
                                                                                                                                              |                                                                                                                                              
aaGGGGTTTTGCGCCTGGCTTGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATc                                                                                                                                          >  4:329165/1‑149 (MQ=255)
aaGGGGTTTTGAGCCTCGCTTGAGGGCAATAGTAGTGAGGCTGGATTATTCTTAAAAATACTGTATAAGAATTTTTATATAACCATTTAAGAGAAAACGCCTCTCGTAGGAGCGGGTTTACACGCGAATACTGTGGTAGATGCACCATc                                                                                                                                          >  4:114830/1‑149 (MQ=255)
          gcgcCTGGCTTTAGGGAAATATTAGTTAGGCGGTATTATTATAAAAAATACTGTTAAATAATTTTTATAAAACAATTTAAGACAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTcgcg                                                                                                                                 <  1:185280/148‑1 (MQ=37)
           cgcTTGGCTTGTGGGCAATATTCGGGAGGCGGGCTTTTGTTTAAAAATAATTTTTAAGAATTTTTATATTACCATTTACGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCgg                                                                                                                                <  5:253498/148‑1 (MQ=37)
           cgcCTGGCTTGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTAAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATGCGATGGTGCATCGACCACCGTATTCGCgg                                                                                                                                <  7:314495/148‑1 (MQ=25)
                   ttGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCCCTGGTAGGAGCGGGTTTACCCGCGAATGCGATGGTGCATCGACCACCGTATTCGCGGGTAAA‑c                                                                                                                         >  6:315054/1‑146 (MQ=21)
                   ttGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCCCTGGTAGGAGCGGGTTTACCCGCGAATGCGATGGTGCATCGACCACCGTATTCGCGGGTAAA‑c                                                                                                                         <  5:315054/146‑1 (MQ=21)
                     gAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAA‑CCCGc                                                                                                                     <  3:114830/148‑1 (MQ=255)
                       gggCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCa                                                                                                                                       <  2:196085/129‑1 (MQ=255)
                       gggCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCa                                                                                                                                       >  1:196085/1‑129 (MQ=255)
                         gCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTAACCGCGAATAAGGTGGTCGATTAACCATAGCATTAGCCGGTAAA‑ACCgataaa                                                                                                                 >  7:52105/1‑143 (MQ=255)
                           aaTAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATGCGATGGTGCATCGACCACCGTATTCGCgg                                                                                                                                >  1:89371/1‑132 (MQ=25)
                           aaTAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATGCGATGGTGCATCGACCACCGTATTCGCgg                                                                                                                                <  2:89371/132‑1 (MQ=25)
                           aaTAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAA‑CCCGCTCCTAc                                                                                                               >  5:125001/1‑148 (MQ=255)
                                        cGGTATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGTTAAA‑CCCGCTCCTACCAGGGGCCATATgg                                                                                                 <  1:220615/149‑1 (MQ=255)
                                          ggATTATTCTTAAAAATACTGTTTAAGAATTTTAATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGGCGATGCACCATCGCATTCGCGGGTAAACCCCGCTCCTACCAGGGGCCATATggg                                                                                                <  1:3793/149‑1 (MQ=255)
                                                ttCTTACAAATCCTGTTTAAGAAGTGTTATCTAACGATTTACTATACCCCGCCTCTGGTCGGAGCGGGTTTACCCGCGAATAGGGTGGGCGCGGCCCCCTCGCATGCGCGGGTAAA‑CCCGCGCCTACCAGGGGCCATAGGGGCCTTCAg                                                                                         <  6:376251/149‑1 (MQ=255)
                                                ttCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTAACCGCGAATACGGTGGTGGATGGACCATCGCATTAGCGGGTAAA‑CCCGCTCCTACCAGGGGCCATATGGGGCTTGAg                                                                                         >  3:180696/1‑149 (MQ=37)
                                                                  aaGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCAGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAA‑CCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTcg                                                                       >  7:98626/1‑149 (MQ=255)
                                                                            ataaaACAATTTATCACACCAGGCCTCTGTTTGGTGGTGGTTAACCCCCTATTAGGGTGGTCTATTCACCATCGCATTCGCGGTTAAC‑CCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTg                                                              <  4:180696/148‑1 (MQ=255)
                                                                              ataACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCTTTCGCGGGTAAA‑c                                                                                                                         <  4:208734/87‑1 (MQ=255)
                                                                              ataACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAA‑c                                                                                                                         >  3:208734/1‑87 (MQ=255)
                                                                                aaCCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAA‑c                                                                                                                         >  3:341588/1‑85 (MQ=255)
                                                                                aaCCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAA‑c                                                                                                                         <  4:341588/85‑1 (MQ=255)
                                                                                aaCCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAA‑CCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTAc                                                           >  1:258268/1‑147 (MQ=255)
                                                                                aaCCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAA‑CCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTAc                                                           >  5:290320/1‑147 (MQ=255)
                                                                                     tttAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAA‑CCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCa                                                    <  2:258268/149‑1 (MQ=255)
                                                                                        aaGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGGATACGGTGGTCGATGGACCATCGCATTCGCGGGTTAA‑CCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGGGGTTGTGGGGCTCGCGGAtgttgt                                                             >  5:33150/1‑137 (MQ=25)
                                                                                                    ctctGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAA‑CCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCgg                                       >  5:360553/1‑147 (MQ=255)
                                                                                                       tGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAA‑CCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAg                                    <  6:290320/147‑1 (MQ=255)
                                                                                                       tGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAA‑CCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAg                                    <  6:125001/147‑1 (MQ=255)
                                                                                                            ggAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAA‑CCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAAcagc                             <  3:329165/149‑1 (MQ=255)
                                                                                                                         ccGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAA‑CCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAg                                    <  4:176952/129‑1 (MQ=255)
                                                                                                                         ccGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAA‑CCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAg                                    >  3:176952/1‑129 (MQ=255)
                                                                                                                          cgcgAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAA‑CCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGt               >  1:129756/1‑149 (MQ=255)
                                                                                                                           gcgAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAA‑CCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTc              >  1:334974/1‑149 (MQ=255)
                                                                                                                             gAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAA‑CCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGc            >  1:302211/1‑149 (MQ=255)
                                                                                                                                    gtggtCGATGCACCATCGCATTCGCGGGTAAA‑CCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACgat      >  4:41319/1‑148 (MQ=255)
                                                                                                                                       gtCGATGCACCATCGCATTCGCGGGTAAA‑CCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCCCGATGATc  >  4:48300/1‑149 (MQ=255)
                                                                                                                                         cGATGCACCATCGCATTCGCGGGTAAA‑CCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCTTCAGCAAGGTCGCCACgat      <  4:82932/143‑1 (MQ=255)
                                                                                                                                         cGATGCACCATCGCATTCGCGGGTAAA‑CCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACgat      >  3:82932/1‑143 (MQ=255)
                                                                                                                                              |                                                                                                                                              
AAGGGGTTTTGCGCCTGGCTTGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAA‑CCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATC  >  NC_002947/5897641‑5897924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑