| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,897,791 | C→T | 15.2% | intergenic (‑191/+43) | PP_5172 ← / ← PP_5173 | hypothetical protein/RND family transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,897,791 | 0 | C | T | 15.2% | 107.3 / 10.9 | 46 | intergenic (‑191/+43) | PP_5172/PP_5173 | hypothetical protein/RND family transporter |
| Reads supporting (aligned to +/- strand): ref base C (20/19); new base T (3/4); total (23/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.55e-01 | |||||||||||
GCGCCTGGCTTGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAA‑CCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCG > NC_002947/5897651‑5897939 | gcgcCTGGCTTTAGGGAAATATTAGTTAGGCGGTATTATTATAAAAAATACTGTTAAATAATTTTTATAAAACAATTTAAGACAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTcgcg < 1:185280/148‑1 (MQ=37) cgcTTGGCTTGTGGGCAATATTCGGGAGGCGGGCTTTTGTTTAAAAATAATTTTTAAGAATTTTTATATTACCATTTACGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCgg < 5:253498/148‑1 (MQ=37) cgcCTGGCTTGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTAAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATGCGATGGTGCATCGACCACCGTATTCGCgg < 7:314495/148‑1 (MQ=25) ttGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCCCTGGTAGGAGCGGGTTTACCCGCGAATGCGATGGTGCATCGACCACCGTATTCGCGGGTAAA‑c < 5:315054/146‑1 (MQ=21) ttGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCCCTGGTAGGAGCGGGTTTACCCGCGAATGCGATGGTGCATCGACCACCGTATTCGCGGGTAAA‑c > 6:315054/1‑146 (MQ=21) gAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAA‑CCCGc < 3:114830/148‑1 (MQ=255) gggCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCa < 2:196085/129‑1 (MQ=255) gggCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCa > 1:196085/1‑129 (MQ=255) gCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTAACCGCGAATAAGGTGGTCGATTAACCATAGCATTAGCCGGTAAA‑ACCgataaa > 7:52105/1‑143 (MQ=255) aaTAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATGCGATGGTGCATCGACCACCGTATTCGCgg > 1:89371/1‑132 (MQ=25) aaTAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATGCGATGGTGCATCGACCACCGTATTCGCgg < 2:89371/132‑1 (MQ=25) aaTAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAA‑CCCGCTCCTAc > 5:125001/1‑148 (MQ=255) cGGTATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGTTAAA‑CCCGCTCCTACCAGGGGCCATATgg < 1:220615/149‑1 (MQ=255) ggATTATTCTTAAAAATACTGTTTAAGAATTTTAATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGGCGATGCACCATCGCATTCGCGGGTAAACCCCGCTCCTACCAGGGGCCATATggg < 1:3793/149‑1 (MQ=255) ttCTTACAAATCCTGTTTAAGAAGTGTTATCTAACGATTTACTATACCCCGCCTCTGGTCGGAGCGGGTTTACCCGCGAATAGGGTGGGCGCGGCCCCCTCGCATGCGCGGGTAAA‑CCCGCGCCTACCAGGGGCCATAGGGGCCTTCAg < 6:376251/149‑1 (MQ=255) ttCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTAACCGCGAATACGGTGGTGGATGGACCATCGCATTAGCGGGTAAA‑CCCGCTCCTACCAGGGGCCATATGGGGCTTGAg > 3:180696/1‑149 (MQ=37) aaGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCAGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAA‑CCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTcg > 7:98626/1‑149 (MQ=255) ataaaACAATTTATCACACCAGGCCTCTGTTTGGTGGTGGTTAACCCCCTATTAGGGTGGTCTATTCACCATCGCATTCGCGGTTAAC‑CCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTg < 4:180696/148‑1 (MQ=255) ataACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCTTTCGCGGGTAAA‑c < 4:208734/87‑1 (MQ=255) ataACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAA‑c > 3:208734/1‑87 (MQ=255) aaCCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAA‑c < 4:341588/85‑1 (MQ=255) aaCCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAA‑c > 3:341588/1‑85 (MQ=255) aaCCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAA‑CCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTAc > 1:258268/1‑147 (MQ=255) aaCCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAA‑CCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTAc > 5:290320/1‑147 (MQ=255) tttAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAA‑CCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCa < 2:258268/149‑1 (MQ=255) aaGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGGATACGGTGGTCGATGGACCATCGCATTCGCGGGTTAA‑CCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGGGGTTGTGGGGCTCGCGGAtgttgt > 5:33150/1‑137 (MQ=25) ctctGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAA‑CCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCgg > 5:360553/1‑147 (MQ=255) tGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAA‑CCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAg < 6:290320/147‑1 (MQ=255) tGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAA‑CCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAg < 6:125001/147‑1 (MQ=255) ggAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAA‑CCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAAcagc < 3:329165/149‑1 (MQ=255) ccGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAA‑CCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAg > 3:176952/1‑129 (MQ=255) ccGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAA‑CCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAg < 4:176952/129‑1 (MQ=255) cgcgAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAA‑CCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGt > 1:129756/1‑149 (MQ=255) gcgAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAA‑CCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTc > 1:334974/1‑149 (MQ=255) gAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAA‑CCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGc > 1:302211/1‑149 (MQ=255) gtggtCGATGCACCATCGCATTCGCGGGTAAA‑CCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACgat > 4:41319/1‑148 (MQ=255) gtCGATGCACCATCGCATTCGCGGGTAAA‑CCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCCCGATGATc > 4:48300/1‑149 (MQ=255) cGATGCACCATCGCATTCGCGGGTAAA‑CCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCTTCAGCAAGGTCGCCACgat < 4:82932/143‑1 (MQ=255) cGATGCACCATCGCATTCGCGGGTAAA‑CCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACgat > 3:82932/1‑143 (MQ=255) catcgaccaccGTATTCGCGGGTAAA‑CCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCg > 3:157659/6‑149 (MQ=255) gaccaccGTATTCGCGGGTAAA‑CCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCaa < 4:157659/148‑1 (MQ=255) ccATCGCATTCGCGGGTAAA‑CCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAAt > 5:22032/1‑148 (MQ=255) cATCGCATTCGCGGGTAAA‑CCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGc > 6:170457/1‑143 (MQ=255) cATCGCATTCGCGGGTAAA‑CCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGc < 5:170457/143‑1 (MQ=255) tCGCATTCGCGGGTAAA‑CCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATg < 2:302211/149‑1 (MQ=255) tCGCATTCGCGGGTAAA‑CCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATg < 8:98626/149‑1 (MQ=255) cATTCGCGGGTAAA‑CCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCAc < 7:37125/85‑1 (MQ=255) cATTCGCGGGTAAA‑CCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCAc > 8:37125/1‑85 (MQ=255) cATTCGCGGGTAAA‑CCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCg > 7:122458/1‑149 (MQ=255) | GCGCCTGGCTTGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAA‑CCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCG > NC_002947/5897651‑5897939 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |