Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,897,942 G→C 9.6% A982A (GCC→GCG)  PP_5173 ← RND family transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,897,9420GC 9.6% 156.5 / 8.0 52A982A (GCC→GCG) PP_5173RND family transporter
Reads supporting (aligned to +/- strand):  ref base G (26/21);  new base C (3/2);  total (29/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.03e-01

CGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGTGGTTGGTGGCCTCCACCACCGCATCCCACGGCGACAAGCCCTGGGCTTCGAACTCGTC  >  NC_002947/5897795‑5898088
                                                                                                                                                   |                                                                                                                                                  
cgcgGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAg                                                                                                                                                    <  6:22032/148‑1 (MQ=255)
      tAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATgg                                                                                                                                             <  5:345322/149‑1 (MQ=255)
          cccGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTTTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCCTACGCCATgattg                                                                                                                                          <  8:177117/148‑5 (MQ=255)
          cccGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGCACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCCTACGCCATgattgg                                                                                                                                         >  7:177117/1‑144 (MQ=255)
          cccGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGTAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGcc                                                                                                                                         >  8:211733/1‑149 (MQ=255)
          cccGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGcc                                                                                                                                         <  2:334974/149‑1 (MQ=255)
                 cTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGaa                                                                                                                                   <  2:129756/148‑1 (MQ=255)
                      aGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTc                                                                                                                             <  3:41319/149‑1 (MQ=255)
                        gggCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTcgc                                                                                                                           <  6:360553/149‑1 (MQ=255)
                           ccATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGGGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTg                                                                                                                        >  7:184105/1‑149 (MQ=255)
                              tatGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGTAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCaa                                                                                                                     <  7:211733/149‑1 (MQ=255)
                               atGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGccc                                                                                                                                        >  3:292126/1‑129 (MQ=255)
                               atGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGccc                                                                                                                                        <  4:292126/129‑1 (MQ=255)
                                     cTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGAt                                                                                                              >  1:47705/1‑149 (MQ=255)
                                         aGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCAt                                                                                                           <  8:122458/148‑1 (MQ=255)
                                                     gCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTcgcc                                                                                              >  8:353739/1‑149 (MQ=255)
                                                          tCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGc                                                                                                         >  5:355764/1‑133 (MQ=255)
                                                          tCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGc                                                                                                         <  6:355764/133‑1 (MQ=255)
                                                                  cTTGTACCAGGCCACGTACAGTGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTca                                                                                 >  4:358411/1‑149 (MQ=255)
                                                                  cTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGt                                                                                   >  1:293054/1‑147 (MQ=255)
                                                                     gTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTcagc                                                                               <  8:184105/148‑1 (MQ=255)
                                                                       aCCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTcagcag                                                                             >  7:139080/1‑148 (MQ=255)
                                                                        ccAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGa                                                                           >  5:150264/1‑149 (MQ=255)
                                                                         cAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGAt                                                                          >  1:75537/1‑149 (MQ=255)
                                                                             ccACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATcggc                                                                      <  7:353739/149‑1 (MQ=255)
                                                                              cACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAgg                                                                                                                                                   <  6:87929/71‑1 (MQ=255)
                                                                              cACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAgg                                                                                                                                                   >  5:87929/1‑71 (MQ=255)
                                                                              cACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATgg                                                                                                                                             <  3:121058/77‑1 (MQ=255)
                                                                              cACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATgg                                                                                                                                             >  4:121058/1‑77 (MQ=255)
                                                                                   aCAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGt                                                                >  3:292229/1‑149 (MQ=255)
                                                                                     aGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACCCCTCGCGTGCAATCGGGATCATGCCCAGGCACGCCGCCGCCGCCGTCAGCAGGATCGGCCGGCGTCg                                                              >  3:162405/1‑149 (MQ=255)
                                                                                     aGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCg                                                              >  5:78455/1‑149 (MQ=255)
                                                                                       tgcCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCggt                                                            <  3:358411/148‑1 (MQ=255)
                                                                                              tggAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGtggttggt                                                     >  6:31584/2‑149 (MQ=255)
                                                                                              aGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGtggttggt                                                     >  5:324993/1‑149 (MQ=255)
                                                                                                     gcagcGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGTGGTTGGTGGCCTc                                               >  8:113902/1‑148 (MQ=255)
                                                                                                        gcGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCCTACGCCATgat                                                                                                                                            <  8:179285/52‑3 (MQ=255)
                                                                                                        gcGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCCTACGCCATgat                                                                                                                                            >  7:179285/1‑50 (MQ=255)
                                                                                                           tCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGTGGTTGGTGGCCTCCaccacc                                        >  8:137257/1‑149 (MQ=255)
                                                                                                              gCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGTGGTTGGTGGCCTCCACCACCGc                                      >  3:297407/1‑148 (MQ=255)
                                                                                                                 aGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGTGGTTGGTGGCCTc                                               <  4:127133/136‑1 (MQ=255)
                                                                                                                 aGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGTGGTTGGTGGCCTc                                               >  3:127133/1‑136 (MQ=255)
                                                                                                                    tCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGTGGTTGGTGGCCTCCACCACCGCATCCCAc                               <  2:293054/149‑1 (MQ=255)
                                                                                                                     cGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGTGGTTGGTGGCCTCCACCACCGCATCCCACg                              >  7:179899/1‑149 (MQ=255)
                                                                                                                         aCGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGTGGTTGGTGGCCTCCACCACCGCATCCCACGGCGa                          <  2:47705/149‑1 (MQ=255)
                                                                                                                               atCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGTGGTTGGTGGCCTCCACCACCGCATCCCACGGCGACAAGcc                    <  6:324993/149‑1 (MQ=255)
                                                                                                                                   cGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATcggcc                                                                     >  5:349785/1‑96 (MQ=255)
                                                                                                                                   cGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATcggcc                                                                     <  6:349785/96‑1 (MQ=255)
                                                                                                                                        aTCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGTGGTTGGTGGCCTCCACCACCGCATCCCACGGCGACAAGCCCTGGGCTTc           <  5:31584/149‑1 (MQ=255)
                                                                                                                                         tCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGTGGTTGGTGGCCTCCACCACCGCATCCCACGGCGACAAGCCCTGGGCTTCg          <  7:113902/149‑1 (MQ=255)
                                                                                                                                           aTGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGTGGTTGGTGGCCTCCACCACCGCATCCCACGGCGACAAGCCCTGGGCTTCGaa        >  2:18832/1‑149 (MQ=255)
                                                                                                                                           aTGGCCTACGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGTGGTTGGTGGCCTCCACCACCGCATCCCACGGCGACAAGCCCTGGGCTTCGaa        >  5:61055/1‑149 (MQ=255)
                                                                                                                                             ggCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGTGGTTGGTGGCCTCCACCACCGCATCCCACGGCGACAAGCCCTGGGCTTCGAACt      >  7:245634/1‑149 (MQ=255)
                                                                                                                                              gCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCT‑‑‑CGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGTGGTTGGTGGCCTCCACCACCGCATCCCACGGCGACAAGCCCTGGGCTTCGAACTCGTc  >  4:311244/1‑149 (MQ=255)
                                                                                                                                                 tAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGTGGTTGGTGGCCTCCACCACCGCATCCCACGGCGACAAGCCCTGGGCTTCGAACTCGTc  <  8:139080/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATCCCGCCAATCATGGCGTAGGCCATGGGGCCCCAGAACACCTCGCGTGCAATCGGGATCATGCCCAGGCTCGCCGCCGCCGCGGTCAGCAGGATCGGCCGGCGTCGGTGGTTGGTGGCCTCCACCACCGCATCCCACGGCGACAAGCCCTGGGCTTCGAACTCGTC  >  NC_002947/5897795‑5898088

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑