Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,899,957	C→G	7.7%	V311L (GTG→CTG)	PP_5173 ←	RND family transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,899,957	0	C	G	7.7%	163.1 / 3.8	52	V311L (GTG→CTG)	PP_5173	RND family transporter
Reads supporting (aligned to +/- strand):  ref base C (24/24);  new base G (2/2);  total (26/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01

GCACGCCAAGACTGACAAAGCTCACGGCCAGCACGATCACCACCGCCTCGAACAGTGCGCTGGTGAAACCGCCTACCGCCTGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCAC‑‑CTGG‑TCCATGCGCTTTTTCAACGCCGCACCGAACACCTGGATGTTGCCGCCGGCCTTCATGCCGATGGCGAGGCCAATGGCTGTCTGGCCGTTGTAACGGAACATTGGCGAAGGCGGGTCGACGTAGCCGCGCTCGATGTCGGC  >  NC_002947/5899810‑5900101                                                                                                                                                    |                                                                                                                                                    gcACGCCAAGACTGACAAAGCTGACGGCCAGCACGATCACCACCGCCTCGAACAGTGCGCTGGTGAAACCGCCTAGCGCCTTCTTGACCACCACGGCCTGGTCAGAAACTGTGTGTACACCGACACCCACCGGCAGGTCCTTGaccac‑‑c                                                                                                                                                  <  5:335855/149‑1 (MQ=255) gcACGCCAAGACTGACAAAGCTCACGGCCAGCACGATCACCACCGCCTCGAACAGTGCGCTGGTGAAACCGCCTACCGCCTGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGaccac‑‑c                                                                                                                                                  >  8:53602/1‑149 (MQ=255) gcACGCCAAGACTGACAAAGCTCACGGCCAGCACGATCACCACCGCCTCGAACAGTGCGCTGGTGAAACCGCCTACCGCCTGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGaccac‑‑c                                                                                                                                                  <  5:310879/149‑1 (MQ=255)     gCCAAGACTGACAAAGCTCACGGCCAGCACGATCACCACCGCCTCGAACAGTGCGCTGGTGAAACCGCCTACCGCCTGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCAC‑‑CTGG‑t                                                                                                                                             >  6:172810/1‑149 (MQ=255)             tGACAAAGCTCACGGCCAGCACGATCACCACCGCCTCGAACAGTGCGCTGGTGAAACCGCCTACCGCCTGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCAG‑‑GTGG‑TCCag                                                                                                                                         >  6:132154/1‑144 (MQ=255)             tGACAAAGCTCACGGCCAGCACGATCACCACCGCCTCGAACAGTGCGCTGGTGAAACCGCCTACCGCCTGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCAG‑‑GTGG‑TCCag                                                                                                                                         <  5:132154/145‑2 (MQ=255)             tGACAAAGCTCACGGCCAGCACGATCACCACCGCCTCGAACAGTGCGCTGGTGAAACCGCCTACCGCCTGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCAC‑‑CTGG‑TCCATgcgc                                                                                                                                     >  3:212929/1‑149 (MQ=255)                cAAAGCTCACGGCCAGCACGATCACCACCGCCTCGAACAGTGCGCTGGTGAAACCGCCTACCGCCTGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCAC‑‑CTGG‑TCCATGCGCttt                                                                                                                                  <  1:202111/149‑1 (MQ=255)                 aaaGCTCACGGCCAGCACGATCACCACCGCCTCGAACAGTGCGCTGGTGAAACCGCCTACCGCCTGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCAC‑‑CTGG‑TCCATGCGCtttt                                                                                                                                 >  6:80551/1‑149 (MQ=255)                  ccgCTCACGCCCAGCACGACCACCACCGCCCCGAACCGCGCGCCCCTGAAACCGCCCCCCGCCCCCCCCACCCCCCCGCCCTGGTCAGAACCCGGGCGCACACCGACACCCACCCGCAGGTCCTGGACCAC‑‑CTGG‑TCCATGCGCctttt                                                                                                                                <  4:239327/147‑1 (MQ=255)                  aaGCTCACGGCCAGCACGATCACCACCGCCTCGAACAGTGCGCTGGTGAAACCGCCTACCGCCTGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCAC‑‑CTGG‑TCCATGCGCttttt                                                                                                                                <  8:12119/149‑1 (MQ=255)                        aCGGCCAGCACGATCACCACCGCCTCGAAAAGTGCGCTGGTGAAACCGCCTACCGCCTGCTTGACCACCAAGGCCTGGACAGAAACGCAGTGTACACCGACACCCACCGGCAGGTCCTGGAACAC‑‑CTGG‑TCCATGCGCTTTATCAAcgc                                                                                                                          >  2:293833/1‑149 (MQ=255)                          ggCCAGCACGATCACCACCGCCTCGAACAGTGCGCTGGTGAAACCGCCTACCGCCTGCTTGACCACCACGGCCTGGGCAGATACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCAC‑‑CTGG‑TCCATGCGCTTTTTCAAcgccg                                                                                                                        <  1:11885/149‑1 (MQ=255)                                         caccGCCTCGAACAGTGCGCTGGTGAAACCGCCTACCGCCTGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCAC‑‑CTGG‑TCCATGCGCTTTTTCAACGCCGCACCGAACACCTGGa                                                                                                         >  1:228366/1‑149 (MQ=255)                                         caccGCCTCGAACAGTGCGCTGGTGAAACCGCCTACCGCCTGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCAC‑‑CTGG‑TCCATGCGCTTTTTCAACGCCGCACCGAACACCTGGa                                                                                                         <  4:212929/149‑1 (MQ=255)                                                    aCAGTGCGCTGGTGAAACCGCCTACCGCCTGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCAC‑‑CTGG‑TCCATGCGCTTTTTCAACGCCGCACCGAACACCTGGATGTTGccgccg                                                                                              <  6:142483/149‑1 (MQ=255)                                                      aGTGCGCTGGTGAAACCGCCTACCGCCTGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCAC‑‑CTGG‑TCCATGCGCTTTTTCAACGCCGCACCGAACACCTGGATGTTGCCGCCGGc                                                                                            <  3:304908/149‑1 (MQ=255)                                                        tGCGCTGGTGAAACCGCCTACCGCCTGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCAC‑‑CTGG‑TCCATGCGCTTTTTCAACGCCGCACCGAACACCTGGATGTTGCCGCCGGCCt                                                                                          <  2:228366/149‑1 (MQ=255)                                                        tGCGCTGGTGAAACCGCCTACCGCCTGCTTGACAACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGCTCCTGCACCAC‑‑CTCG‑TCCAAGCGCTTTTACAACGCCCCACCGAACACCTGGATGTTGACACCGGcc                                                                                           >  4:221301/1‑148 (MQ=255)                                                               gTGAAACCGCCTACCGCCTGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCAC‑‑CTGG‑TCCATGCGCTTTTTCAACGCCGCACCGAACACCTGGATGTTGCCGCCGGCCTTCATg                                                                                     <  5:172810/147‑1 (MQ=255)                                                               gTGAAACCGCCTACCGCCTGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCAC‑‑CTGG‑TCCATGCGCTTTTTCAACGCCGCACCGAACACCTGGATGTTGCCGCCGGCCTTCATGc                                                                                    <  3:221301/148‑1 (MQ=255)                                                                    accgcctaccgcctGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCAC‑‑CTGG‑TCCATACGCTTTTTCAACGCCGCACCGAACACCTGGATGTTGCCGCCGGCCTTCATGCCGATgg                                                                              >  8:136541/1‑149 (MQ=255)                                                                             cgcctGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCAC‑‑CTGG‑TCCATGCGCTTTTTCAACGCCGCACCGAACACCTGGATGTTGCCGCCGGCCTTCATGCCGATGGCGAGGCCaa                                                                     <  7:53602/149‑1 (MQ=255)                                                                                         caccacGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCACAACTGG‑TCCATGCGCTTTTTCAACGCCGCACCGAACACCTGGATGTTGCCGCCGGCCTTCATGCCGATGGCGAGGCCAATGGCTGTCTg                                                           >  1:106558/1‑149 (MQ=255)                                                                                                   tGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCAC‑‑CTGG‑TCCATGCGCTTTTTCAACGCCGCACCGAACACCTGGATGTTGCCGCCGGCCTTCATGCCGATGGCGAGGCCAATGGCTGTCTGGCCGTTGTAACg                                               >  4:340991/1‑149 (MQ=255)                                                                                                      tCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCAC‑‑CTGG‑TCCATGCGCTTTTTCAACGCCGCACCGAACACCTGGATGTTGCCGCCGGCCTTCATGCCGATGGCGAGGCCAATGGCTGTCTGGCCGTTGTAACGGaa                                            <  2:224043/149‑1 (MQ=255)                                                                                                        aGAAACGGTGTGTACACCGCCACCCACCGGCAGGTCCTGGACCAC‑‑CTGG‑TCCATGCGCTTTTTCAACGCCGCACCGAACACCTGGATGTTGCCGCCGGCCTTCATGCCGATGGCGAGGCCAATGGCTGTCTGGCCGTTGt                                                   >  5:306902/1‑140 (MQ=255)                                                                                                        aGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCAC‑‑CTGG‑TCCATGCGCTTTTTCAACGCCGCACCGAACACCTGGATGTTGCCGCCGGCCTTCATGCCGATGGCGAGGCCAATGGCTGTCTGGc                                                         <  4:295891/134‑1 (MQ=255)                                                                                                        aGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCAC‑‑CTGG‑TCCATGCGCTTTTTCAACGCCGCACCGAACACCTGGATGTTGCCGCCGGCCTTCATGCCGATGGCGAGGCCAATGGCTGTCTGGc                                                         >  3:295891/1‑134 (MQ=255)                                                                                                        aGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCAC‑‑CTGG‑TCCATGCGCTTTTTCAACGCCGCACCGAACACCTGGATGTTGCCGCCGGCCTTCATGCCGATGGCGAGGCCAATGGCTGTCTGGCCGTTGt                                                   <  6:306902/140‑1 (MQ=255)                                                                                                        aGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCAC‑‑CTGG‑TCCATGCGCTTTTTCAACGCCGCACCGAACACCTGGATGTTGCCGCCGGCCTTCATGCCGATGGCGAGGCCAATGGCTGTCTGGCCGTTGTAACGGAACa                                          >  6:325491/1‑149 (MQ=255)                                                                                                          aaaCGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCAC‑‑CTGGTTCCATGCGCTTTTTAAACGCCGCACCGAAAACCTGGATGATGCCGCCGGCCTACATGCCGATGGCGAGGCCAATGCCTGTCTGGCCGTTGTAACGCAACAt                                         >  1:273720/1‑149 (MQ=255)                                                                                                          aaaCGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCAC‑‑CTGG‑TCCATGCGCTTTTTCAACGCCGCACCGAACACCTGGATGTTGCCGCCGGCCTTCATGCCGATGGCGAGGCCAATGGCTGTCTGGCCGTTGTAACGGAACAtt                                        >  2:219745/1‑149 (MQ=255)                                                                                                           aaCGGTGTGTACACCGACACCCACAGGCAGGTCCTGGACCAC‑‑CTTG‑TCCATGCGCTTTTTCAACGCCGCACCGAACACCTGGATGTTGCCGCCGGCCTTCATGCCGATGGCGAGGCCAATTGCTGTCTGGCCGTTGTAACGGAACATTg                                       <  2:273720/149‑1 (MQ=255)                                                                                                                tgtgtACACCGACACCCACCGGCAGGTACTGGACCAC‑‑CTGG‑TCCATGCGCTTTTTCAACGCCGCACCGATCACCTGGATTTTGCCGCCGGCATTCATGCCGATGGCGAGGCCAATGGCTGTCTGGCCGTTGTAACGGAACATTgg                                      <  1:293833/145‑1 (MQ=255)                                                                                                                 ctgtACACCGACACCCACCGGCAGGTCCTGGCCCAC‑‑CTGG‑TCCATGCGCTTTTTCAACGCCGCACCGAACACCTGGATGTTGCCGCCGGCCTTCATGCCGATGGCGAGGCCAATGGCTGTCTGGCCGTTGTAACGGAACATTGGCGAAg                                 <  8:213407/148‑1 (MQ=255)                                                                                                                  tgtACACCGACACCCACCGGCAGGTCCTGGACCAC‑‑CTGG‑TCCATGCGCTTTTTCAACGCCGCACCGAACACCTGGATGTTGCCGCCGGCCTTCATGCCGATGGCGAGGCCAATGGCTGTCTGGCCGTTGTAACGGAACATTGGCGAAg                                 <  8:207889/148‑1 (MQ=255)                                                                                                                       acCGACACCCACCGGCAGGTCCTGGACCAC‑‑CTGG‑TCCATGCGCTTTTTCAACGCCGCACCGAACACCTGGATGTTGCCGCCGGCCTTCATGCCGATGGCGAGGCCAATGGCTGTCTGGCCGTTGTAACGGAACATTGGCGAAGGCGGGt                           >  6:4790/1‑149 (MQ=255)                                                                                                                         cGACACCCACCGGCAGGTCCTGGACCAC‑‑CTGG‑TCCATGCGCTTTTTCAACGCCGCACCGAACACCTGGATGTTGCCGCCGGCCTTCATGCCGATGGCGAGGCCAATGGCTGTCTGGCCGTTGTAACGGAACATTGGCGAAGGCGGGTc                          >  6:237245/1‑148 (MQ=255)                                                                                                                           acacCCACCGGCAGGTCCTGGACCAC‑‑CTGG‑TCCATGCGCTTTTTCAACGCCGCACCGAACACCTGGATGTTGCCGCCGGCCTTCATGCCGATGGCGAGGCCAATGGCTGTCTGGCCGtt                                                     >  3:332122/1‑119 (MQ=255)                                                                                                                           acacCCACCGGCAGGTCCTGGACCAC‑‑CTGG‑TCCATGCGCTTTTTCAACGCCGCACCGAACACCTGGATGTTGCCGCCGGCCTTCATGCCGATGGCGAGGCCAATGGCTGTCTGGCCGtt                                                     <  4:332122/119‑1 (MQ=255)                                                                                                                           acacCCACCGGCAGGTCCTGGACCAC‑‑CTGG‑TCCATGCGCTTTTTCAACGCCGCACCGAACACCTGGATGTTGCCGCCGGCCTTCATGCCGATGGCGAGGCCAATGGCTGTCTGGCCGTTGTAACGGAACATTGGCGAAGGCGGGTCGAc                       <  5:80551/149‑1 (MQ=255)                                                                                                                           acacCCACCGGCAGGTCCTGGACCAC‑‑CTGG‑TCCATGCGCTTTTTCAACGCCGCACCGAACACCTGGATGTTGCCGCCGGCCTTCATGCCGATGGCGAGGCCAATGGCTGTCTGGCCGTTGTAACGGAACATTGGCGAAGGCGGGTCGAc                       <  4:355111/149‑1 (MQ=255)                                                                                                                               ccaccGGCAGGTCCTGGACCAC‑‑CTGG‑TCCATGCGCTTTTTCAACGCCGCACCGAACACCTGGATGTTGCCGCCGGCCTTCATGCCGATGGCGAGGc                                                                        <  5:237502/96‑1 (MQ=255)                                                                                                                               ccaccGGCAGGTCCTGGACCAC‑‑CTGG‑TCCATGCGCTTTTTCAACGCCGCACCGAACACCTGGATGTTGCCGCCGGCCTTCATGCCGATGGCGAGGc                                                                        >  6:237502/1‑96 (MQ=255)                                                                                                                                 accGGCAGGTCCTGGACCAC‑‑CTGG‑TCCATGCGCTCTTTCAACGCCGCACCGAACACCTGGATGTTGCCGCCGGCCTTCATGCCGATGGCGAGGCCAATGGCTGt                                                              >  1:11050/1‑104 (MQ=255)                                                                                                                                 accGGCAGGTCCTGGACCAC‑‑CTGG‑TCCATGCGCTCTTTCAACGCCGCACCGAACACCTGGATGTTGCCGCCGGCCTTCATGCCGATGGCGAGGCCAATGGCTGt                                                              <  2:11050/104‑1 (MQ=255)                                                                                                                                       aGGTCCTGGACCAC‑‑CTGG‑TCCATGCGCTTTTTCAACGCCGCACCGAACACCTGGATGTTGCCGCCGGCCTTCATGCCGATGGCGAGGCCAATGGCTGTCTGGCCGTTGTAACGGAACATTGGCGAAGGCGGGTCGACGTAGCCGCGCTc           >  7:125492/1‑149 (MQ=255)                                                                                                                                         gTCCTGGACCAC‑‑CTGG‑TCCATGCGCTTTTTCAACGCCGCACCGAACACCTGGATGTTGCCGCCGGCCTTCATGCCGATGGCGAGGCCAATGGCTGTCTGGCCGTTGTAACGGAACATTGGCGAAGGCGGGTCGACGTAGcc                 <  7:174068/141‑1 (MQ=255)                                                                                                                                         gTCCTGGACCAC‑‑CTGG‑TCCATGCGCTTTTTCAACGCCGCACCGAACACCTGGATGTTGCCGCCGGCCTTCATGCCGATGGCGAGGCCAATGGCTGTCTGGCCGTTGTAACGGAACATTGGCGAAGGCGGGTCGACGTAGcc                 >  8:174068/1‑141 (MQ=255)                                                                                                                                         gTCCTGGACCAC‑‑CTGG‑TCCATGCGCTTTTTCAACGCCGCACCGAACACCTGGATGTTGCCGCCGGCCTTCATGCCGATGGCGAGGCCAATGGCTGTCTGGCCGTTGTAACGGAACATTGGCGAAGGCGGGTCGACGTAGCCGCGCTCGa         >  1:178516/1‑149 (MQ=255)                                                                                                                                          tCCTGGACCAC‑‑CTGG‑TCCATGCGCTTTTTCAACGCCGCACCGAACACCTGGATGTTGCCGCCGGCCTTCATGCCGATGGCGAGGCCAATGGCTGTCTGGCCGTTGTAACGGAACATTGGCGAAGGCGGGTCGACGTAGCCGCGCTCGAt        >  6:226707/1‑149 (MQ=255)                                                                                                                                          gcatGGACCAG‑‑GTGG‑TCCATGCGCTTTTTCGACGCCGCACCGAACACCTGGATGTTGCCGCCGGCCTTCATGCCGATGGCGAGGCCAATGGCTGTCTGGCCGTTGTAACGGAACATTGGCGAAGGc                               >  3:113003/4‑126 (MQ=255)                                                                                                                                          gcatGGACCAG‑‑GTGG‑TCCATGCGCTTTTTCGACGCCGCACCGAACACCTGGATGTTGCCGCCGGCCTTCATGCCGATGGCGAGGCCAATGGCTGTCTGGCCGTTGTAACGGAACATTGGCGAAGGc                               <  4:113003/123‑1 (MQ=255)                                                                                                                                             tGGACCAC‑‑CTGG‑TCCATGCGCTTTTTCAACGCCGCACCGAACACCTGGATGTTGCCGCCGGCCTTCATGCCGATGGCGAGGCCAATGGCTGTCTGGCCGTTGTAACGGAACATTGGCGAAGGCGGGTCGACGTAGCCGCGCTCGATGTc     >  3:311822/1‑149 (MQ=255)                                                                                                                                                 ccac‑‑cTGG‑TCCATGCGCTTTTTCAACGCCGCACCGAACACCTGGATGTTGCCGCCGGCCTTCATGCCGATGGCGAGGCCAATGGCTGTCTGGCCGTTGTAACGGAACATTGGCGAAGGCGGGTCGACGTAGCCGCGCTCGATGTCGGc  >  1:23794/1‑148 (MQ=255)                                                                                                                                                    |                                                                                                                                                    GCACGCCAAGACTGACAAAGCTCACGGCCAGCACGATCACCACCGCCTCGAACAGTGCGCTGGTGAAACCGCCTACCGCCTGCTTGACCACCACGGCCTGGTCAGAAACGGTGTGTACACCGACACCCACCGGCAGGTCCTGGACCAC‑‑CTGG‑TCCATGCGCTTTTTCAACGCCGCACCGAACACCTGGATGTTGCCGCCGGCCTTCATGCCGATGGCGAGGCCAATGGCTGTCTGGCCGTTGTAACGGAACATTGGCGAAGGCGGGTCGACGTAGCCGCGCTCGATGTCGGC  >  NC_002947/5899810‑5900101

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑