Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,903,165 A→C 12.6% intergenic (‑129/‑139) PP_5175 ← / → PP_5176 HlyD family secretion protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,903,1650AC12.6% 109.4 / 5.4 46intergenic (‑129/‑139)PP_5175/PP_5176HlyD family secretion protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (19/21);  new base C (2/4);  total (21/25)
Fisher's exact test for biased strand distribution p-value = 6.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.01e-01

CGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACTGTGAGCGTAGTTCAGGGTTCGATAAACATCAC‑‑TGACGGGGGCCATGCTGGCATTTTTGGTGCCTGCGGGGGCCTCTTCGCGGGCGAGCCCGCGAAGAGGCCTCCACAGCCATGTATCAGTATCTGCCATATCCAGAAACCATCAGGCATCGGCCATCCCCGTTCCAACCGCTACCTTTCACAGGGAGGCTCACATTCAAGGAAGATCAAAATGCCGTTAGCCGAATGCCACC  >  NC_002947/5903023‑5903311
                                                                                                                                                |                                                                                                                                                  
cGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACTGTGAGCGTAGTTCAGGGTTCGATAAACATCAC‑‑TGACGGGGGCCATGCTGGCATTTTTGGTGCCTGCGGGGGCCTCTTCGCGGGCGAGCCcgc                                                                                                                                              <  5:10779/149‑1 (MQ=255)
 gCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACTGTGAGCGTAGTTCAGGGTTCGATAAACATCAC‑‑TGACGGGGGCCATGCTGGCATTTTTGGTGCCTGCGGGGGCCTCTTCGCGGGCGAGCCcgc                                                                                                                                              >  5:165657/1‑148 (MQ=255)
   aTCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACTGTGAGCGTAGTTCAGGGTTCGATAAACATCAC‑‑TGACGGGGGCCATGCTGGCATTTTTGGTGCCTGCGGGGGCCTCTTCGCGGGCTCGCCCGCGaa                                                                                                                                           <  2:278113/149‑1 (MQ=255)
   aTCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACTGTGAGCGTAGTTCAGGGTTCGATAAACATCAC‑‑TGACGGGGGCCATGCTGGCATTTTTGGTGCCTGCGGGGGCCTCTTCGCGGGCGAGCCCGCGaa                                                                                                                                           <  2:344742/149‑1 (MQ=255)
   aTCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACTGTGAGCGTAGTTCAGGGTTCGATAAACATCAC‑‑TGACGGGGGCCATGCTGGCATTTTTGGTGCCTGCGGGGCCCTCTTCGCGGGCGAGCCCGCGa                                                                                                                                            >  8:294628/1‑148 (MQ=255)
      aaCGCCATGCTGGCTCCTTGCTTGTGCAAAAATGATTTGTGGTATGCGACTGTGAGCGTAGTTCAGGGTTCGATAAACATCACCTTGACGGGGGCCATGCTGGCATTTTTGGTGCCTGCGGGGGCCTCTTCGCGGGCTCGCCCGCGaa                                                                                                                                           <  1:296472/148‑1 (MQ=255)
       aCGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACTGTGAGCGTAGTTCAGGGTTCGATAAACATCAC‑‑TGACGGGGGCCATGCTGGCATTTTTGGTGCCTGCGGGGGCCTCTTCGCGGGCGAGCCCGCGAAGAgg                                                                                                                                       >  4:320629/1‑149 (MQ=255)
         gCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACTGTGAGCGTAGTTCAGGGTTCAATAAACATCAC‑‑TGACGGGGGCCATGCTGGCATTTTTGGTGCCTGCGGGGGCCTCTTCGCGGGCTCGCCCGCGAAGAGGcc                                                                                                                                     <  6:49589/149‑1 (MQ=255)
                tGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACTGTGAGCGTAGTTCAGGGTTCGATAAACATCAC‑‑TGACGGGGGCCATGCTGGCATTTTTGGTGCCTGCGGGGGCCTCTTCGCGGGCGAGCCCACGAAGAGGCCTCCACAg                                                                                                                              >  1:35896/1‑149 (MQ=255)
                      gtgggttgTGCAATATTTATTTGTGGGATGCGACTGTGAGCGTAGTTCAGGGTTCGATAAACATCCC‑‑TGAGGGGGGCCTTGCTGGCATTTTTGGTGCCTGCGGGGGCCTCTTGGCGGGCGAGCCCGGGAAGAGGCCTCCACAGCCAt                                                                                                                          <  8:20559/142‑1 (MQ=255)
                          cttgTGCACAATTGATTTGTGGTTTGGGACTGTGAGGGTAGTTCTGGGTTCGATAAACATCAC‑‑TGACGGGGGCCTTGCTGGCATTTTTGGGGGCTGTGGTGGCCTCTTCGCGGGCTCGCCCGCGAAGAGGCCCCCGCAGGCaccaaaaa                                                                                                                    <  6:348149/149‑8 (MQ=18)
                          cttgTGCACAAATGATTTGTGGGATGCGACTGTGAGCGTAGTTCAGGGTTCGATAAACATCAC‑‑TGACGGGGGCCATGCTGGCATTTTTGGTGCCTGCGGGGGCCTCTTCGCGGGCGAGCCCGCGAAGAGGCCTCCACAGCCATGTATCa                                                                                                                    <  8:102855/149‑1 (MQ=255)
                           ttgTGCACAAATGATTTGTGGGATGCGACTGTGAGCGTAGTTCAGGGTTCGATAAACATCAC‑‑TGACGGGGGCCATGCTGGCATTTTTGGTGCCTGCGGGGGCCTCTTCGCGGGCGAGCCCGCGAAGAGGCCTCCACAGCCATGTATCAg                                                                                                                   >  7:293412/1‑149 (MQ=255)
                             gtgCACAAATGATTTGTGGGATGCGACTGTGAGCGTAGTTCAGGGTTCGATAAACATCAC‑‑TGACGGGGGCCATGCTGGCATTTTTGGTGCCTGCGGGGGCCTCTTCGCGGGCGAGCCCGCGAagag                                                                                                                                        <  2:70253/126‑1 (MQ=255)
                             gtgCACAAATGATTTGTGGGATGCGACTGTGAGCGTAGTTCAGGGTTCGATAAACATCAC‑‑TGACGGGGGCCATGCTGGCATTTTTGGTGCCTGCGGGGGCCTCTTCGCGGGCGAGCCCGCGAagag                                                                                                                                        >  1:70253/1‑126 (MQ=255)
                               gCACAAATGATTTGTGGGATGCGACTGTGAGCGTAGTTCAGGGTTCGATAAACATCAC‑‑TGACGGGGGCCATGCTGGCATTTTTGGTGCCTGCGGGGGCCTATTCGCGGGCGAGCCCGCGAAGAGGCCACCACACCCATGTATAAGTATc                                                                                                               >  2:270601/1‑149 (MQ=255)
                                cacaAATGATTTGTGGGATGCGACTGTGAGCGTAGTTCAGGGTTCGATAAACATCAC‑‑TGACGGGGGCCATGCTGGCATTTTTGGTGCCTGCGGGGGCCTCTTCGCGGGCGAGCCCGCGAAGAGGCCTCCACAGCCATGTATCAGTATc                                                                                                               <  1:270601/148‑1 (MQ=255)
                                cacaAATGATTTGTGGGATGCGACTGTGAGCGTAGTTCAGGGTTCGATAAACATCAC‑‑TGACGGGGGCCATGCTGGCATTTTTGGTGCCTGCGGGGGCCTCTTAGCGGGCGCGCCCCCGCAGAGGCCTCACCACCCCAGTATGAGTATCt                                                                                                              >  2:307560/1‑149 (MQ=255)
                                  caAATGATTTGTGGGATGCGACTGTGAGCGTAGTTCAGGGTTCGATAAACATCAC‑‑TGACGGGGGCCATGCTGGCATTTTTGGTGCCTGCGGGGGCCTCTTCGCGGGCGAGCCCGCGAAGAGGCCTCCACAGCCATGTATCAGTATCTGc                                                                                                            <  6:165657/149‑1 (MQ=255)
                                   aaaTGATTTGTGGGATGCGACTGTGAGCGTAGTTCAGGGTTCGATAAACATCAC‑‑TGACGGGGGCCATGCTGGCATTTTTGGTGCCTGCGGGGGCCTCTTCGCGGGCGAGCCCGCGAAGAGGCCTCCACAGCCATGTATCAGTATCTgg                                                                                                            >  5:261807/1‑147 (MQ=255)
                                          ttGTGGGATGCGACTGTGAGCGTAGTTCAGGGTTCGATAAACATCAC‑‑TGACGGGGGCCATGCTGGCATTTTTGGTGCCTGCGGGGGCCTCTTCGCGGGCGAGCCCGCGAAGAGGCCTCCACAGCCATGTATCAGTATCTGCCATATcc                                                                                                     <  8:293412/148‑1 (MQ=255)
                                                   gCGACTGTGAGCGTAGTTCAGGGTTCGATAAACATCAC‑‑TGACGGGGGCCATGCTGGCATTTTTGGTGACTGCGGGGGCCTAATACCGGGCGCCGCCGCGAAGCGGCAGACCCAGGCATGTAAGAGATTATGCCATATCAAGTAAccct                                                                                            >  2:120839/1‑146 (MQ=255)
                                                     gACTGTGAGCGTAGTTCAGGGTTCGATAAACATCAC‑‑TGACGGGGGCCATGCTGGCATTTTTGGTGCCTGCGGGGGCCTCTTCGCGGGCGAGCCCACGAAGAGGCCTCCACAGCCATGTATCAGTATCTGCCATATCCAGAAACCATCAg                                                                                         <  2:35896/149‑1 (MQ=255)
                                                     gACTGTGAGCGTAGGTCAGGGTTCGATAAACATCAC‑‑TGACGGGGGCCATGCTGGCATTTTTGGTGCCTGCGGGGGCCTCTTCGCGGGCGAGCCCGCGAAGAGGCCTCCACAGCCATGTATCAGTATCTGGCATATCCAGAAACCATCAg                                                                                         <  6:261807/149‑1 (MQ=255)
                                                          tgAGCGTAGTTCAGGGTTCGATAAACATCAC‑‑TGACGGGGGCCATGCTGGCATTTTTGGTGCCTGCGGGGGCCTCTTCGCGGGCGAGCCCGCGAAGAGGCCTc                                                                                                                                   <  7:105075/102‑1 (MQ=255)
                                                           gctcttcgTTAAGGGTTCTATAACCATCTC‑‑TGACGGGGGCCATGGTGGCATTTTTGGTGCCTGCGGGGTCCTCTTCGCGGGCGAGCCCGCGAAGAGGCCTCCACAGCCATGTATCAGTATCTGCCATATCCAGAAACCATCAGGCATCg                                                                                   <  8:90162/142‑1 (MQ=255)
                                                                tAGTGCAGGGTTCGATAAACATCAC‑‑TGACGGGGGCCATGCTGGCATTTTTGGTGCCTGCGGGGGCCTCTGCGCGGGCGAGCCCGCGaa                                                                                                                                           <  3:43342/88‑1 (MQ=255)
                                                                  gTTCAGGGTTCGATAAACATCAC‑‑TGACGGGGGCCATGCTGGCATTTTTGGTGCCTGCGGGGGCCTCTTCGCGGGCGAGCCCGCGAAGAGGCCTCCACAGCCATGTATCAGTATCTGCCATATCCAGAAACCATCAGGCATCGGCCATc                                                                             <  7:294628/148‑1 (MQ=255)
                                                                             gATAAACATCAC‑‑TGACGGGGGCCATGCTGGCATTTTTGGTGCCTGCGGGGGCCTCATCCCGGGCGAGCCAGCGAAGACGCCTGCACAGCCATGTATCAGTATCTGCCATATCCAGAAACCAAAAGGCATCCGCCAAACCCGTTCCAAcc                                                                 >  7:7163/1‑149 (MQ=255)
                                                                                  aCATCAC‑‑TGACGGGGGCCATGCTGGCATTTTTGGTGCCTGCGGGGGCCTCTTCACCGGCGAGCCCGCGAAGAGGCCTCCACAGCCATGTATCAGTATCTGCCATATCCAGAAACAATCAGGCATCGGCCATCCCCGTTCCAACCGATAc                                                            >  7:109511/1‑149 (MQ=255)
                                                                                   cATCAC‑‑TGACGGGGGCCATGCTGGCATTTTTGGTGCCTGCGGGGGCCACTTAGCGGGCGAGCCCCCGAAGAGGCCACCACAGCCAAGTATAAATATATGCCATATACAGAAAACATCATGCAAAGGCCATACCCCTTACAACCGCTAc                                                            >  8:59145/1‑148 (MQ=255)
                                                                                             aCGGGGGCAATGCTGGCATTTTTGGTGCCTGCGGGGGCCTCTTCGCGGGCGAGCCCGCGAAGAGGCCTCCACAGCCATGTATCAGTATCTGCCATATCCAGAAACCATCAGGCATCGGCCATCCCCGTTCCAACCGCTACCTTTCACAg                                                   <  1:307560/149‑1 (MQ=255)
                                                                                                   gCCTTGCTGGATTTTTTGGTGCGTGCGGGGGACTATTCGCGGGCTATCCCGCTAAGAGGCCTCCACAGCCATGTATCAGTATCTGCCATATCCAGAAACCATCAGGCATCGGCCATCCCCGTTCCAACCGCTACCTTTCACAGGGAGGc                                             <  7:302127/149‑1 (MQ=255)
                                                                                                         cTGGCATTTTTGGTGCCTGCGGGGGCCTCTTCGCGGGCGAGCCCGCGAAGAGGCCTCCACAGCCATGTATCAGTATCTGCCATATCCAGAAACCATCAGGCATCGGCCATCCCCGTTGCAACCGCTACCTTTCACAGGGAGGCTCACAt                                       >  1:67651/1‑149 (MQ=255)
                                                                                                            gCATTTTTGGTGCCTGCGGGGGCCTCATACCGGGCGAGCCCGCGAATAGCCCTACAAACCCAAATATAAGAAAATGCCATAAACAGAAAACAAAACGCAAAGGACATCCCCATTACCAAAGCTAACATTCACAAGGAGGCTaacaata                                     >  8:90099/1‑145 (MQ=255)
                                                                                                                    ggTGCCTGCGGGGGCCTCTTCGCGGGCGAGCCCGCGAAGAGGCCTCCACAGCCATGTATCAGTATCTGCCATATCCAGAAACCATCAGGCATCGGCCATCCCCGTTCCAACCGCTACCTTTCACAGGGAGGCTCACATTCAAGGAAGa                             <  3:320629/148‑1 (MQ=255)
                                                                                                                       gCCTGCGGGGGCCTCTTCGCGGGCGAGCCCGCGAAGAGGCCTCCACAGCCATGTATCAGTATCTGCCATATCCAGAAACCATCAGGCATCGGCCATCCCCGTTCCAACCGCTACCTTTCACAGGGAGGCTCACATTCAAGGAAGATCa                          >  8:81136/1‑148 (MQ=255)
                                                                                                                         cTGCGGGGGCTTCTTCGCGGGCGAGCCCGCGAAGAGGCCTCCACAGCCATGTATCAGTATCTGCCATATCCAGAAACCATCAGGCATCGGCCATCCCCGTTCCAACCGCTACCTTTCACAGGGAGGCTCACATTCAAGGAAGATCaaaa                       <  8:7163/149‑1 (MQ=255)
                                                                                                                             gggggCCTCTTCGCGGGCGAGCCCGCGAAGAGGCCTCCACAGCCATGTATCAGTATCTGCCATATCCAGAAACCATCAGGCATCGGCCATCCCCGTTCCAACCGCTACCTTTCACAGGGAGGCTCACATTCAAGGAAGATCAAAATGcc                   <  7:90099/149‑1 (MQ=255)
                                                                                                                                 gCCTCTTCGCGGGCGAGCCCGCGAAGAGGCCTCCACAGCCATGTATCAGTATCTGCCATATCCAGAAACCATCAGGCATCGGCCATCCCCGTTCCAACCGCTACCTTTCACAGGGAGGCTCACATTCAAGGAAGATCAAAATGCCGt                 >  1:196909/1‑147 (MQ=255)
                                                                                                                                    tctTCGCGGGCGAGCCCGCGAAGAGGCCTCCACAGCCATGTATCAGTATCTGCCATATCCAGAAACCATAAGGCATCGGCCATCCCCGTTCCAACCGCTACCTTTCACAGGGAGGCTCACATTCAAGGAAGATCAAAATGCCGTTAGc             >  5:254946/1‑148 (MQ=255)
                                                                                                                                      ttCGCGGGCGAGCCCGCGAAGAGGCCTCCACAGCCATGTATCAGTATCTGCCATATCCAGAAACCATCAGGCATCGGCCATCCCCGTTCCAACCGCTACCTTTCACAGGGAGGCTCACATTCAAGGAAGATCAAAATGCCGTTAGCCg           >  8:115681/1‑148 (MQ=255)
                                                                                                                                         gcgGGCGAGCCCGCGAAGAGGCCTCCACAGCCATGTATCAGTATCTGCCATATCCAGAAACCATCAGGCATCGGCCATCCCCGTTCCAACCGCTACCTTTCACAGGGAGGCTCACATTCAAGGAAGATCAAAATGCCGTTAGCCGAAt        <  7:115681/148‑1 (MQ=255)
                                                                                                                                          cgGGCGAGCCCGCGAAGAGGCCTCCACAGCCATGTATCAGTATCTGCCATATCCAGAAACCATCAGGCATCGGCCATCCCCGTTCCAACCGCTACCTTTCACAGGGAGGCTCACATTCAAGGAAGATCAAAATGCCGTTAGCCGAATGc      <  7:59145/149‑1 (MQ=255)
                                                                                                                                             gctaGCCCGCTAAGAGGACTCCACAGCCATGTATCAGTATCTGCCATATCCAGAAACCATCAGTCATCGGCCATCCCCGTTCCAACCGCTACCTTTCACAGGGAGGCTCACATTCAAGGAAGATCAAAATGCCGTTAGCCGAATGCCAc   <  1:120839/146‑1 (MQ=255)
                                                                                                                                             gCGAGCCCGCGAAGAGGCCTCCACAGCCATGTATCAGTATCTGCCATATCCAGAAACCATCAGGCATCGGCCATCCCCGTTCCAACCGCTACCTTTCACAGGGAGGCTCACATTCAAGGAAGATCAAAATGCCGTTAGCCGAATGCCAc   >  4:67783/1‑149 (MQ=255)
                                                                                                                                               gAGCCCGCGAAGAGGCCTCCACAGCCATGTATCAGTATCTGCCATATCCAGAAACCATCAGGCATCGGCCATCCCCGTTCCAACCGCTACCTTTCACAGGGAGGCTCACATTCAAGGAAGATCAAAATGCCGTTAGCCGAATGCCAcc  >  7:74526/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
CGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACTGTGAGCGTAGTTCAGGGTTCGATAAACATCAC‑‑TGACGGGGGCCATGCTGGCATTTTTGGTGCCTGCGGGGGCCTCTTCGCGGGCGAGCCCGCGAAGAGGCCTCCACAGCCATGTATCAGTATCTGCCATATCCAGAAACCATCAGGCATCGGCCATCCCCGTTCCAACCGCTACCTTTCACAGGGAGGCTCACATTCAAGGAAGATCAAAATGCCGTTAGCCGAATGCCACC  >  NC_002947/5903023‑5903311

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑