Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,933,845 A→G 7.0% D44D (GAT→GAC)  PP_5201 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,933,8450AG 7.0% 162.8 / 2.6 57D44D (GAT→GAC) PP_5201hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (27/26);  new base G (2/2);  total (29/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.07e-01

CAGCATCATCGGTAGGCAGCAGCAGGACGATCGCCATGTCGTCGCTGGTCAGCTCGCCCTTGACCATTTCCTGCAAGCCGACCAAGGCATTGCGGACACTGTCACCAGGCTCGATTTCCAGCAGTTGGGCGGCATCGGCCAACCAGGCATCGGCATCGAAGCCGGCGCCGGCGCAGCTGCGGCCAATCAGCAGGCCGTGCAGTTCGGCCGGGGTGACAGGATGGCCATTGCTCGAG‑AGCAGCATGGCGAAAGCGTTGTAGGGCGATTGAGTATTGGGCATGGGCAGCTAGGCGCCA  >  NC_002947/5933697‑5933992
                                                                                                                                                    |                                                                                                                                                    
cagcaTCATCGGTAGGCAGCAGCAGGACGATCGCCATGTCGTCGCTGGTCAGCTCGCCCTTGACCATTTCCTGCAAGCCGACCAAGGCATTGCGGACACTGTCACCAGGCTCGATTTCCAGCAGTTGGGCGGCATCGGCCAACCAggca                                                                                                                                                      >  5:121698/1‑149 (MQ=255)
   catcatCGGTAGGCAGCAGCAGGACGATCGCCATGTCGTCGCTGGTCAGCTCGCCCTTGACCATTTCCTGCAAGCCGACCAAGGCATTGCGGACACTGTCACCAGGCTCGATTTCCAGCAGTTGGGCGGCATCGGCCAACCAggcatcg                                                                                                                                                   <  8:267191/149‑1 (MQ=255)
     tcctccgTAGGCAGCATCACGACGAGCGCCATGTCGTCGCCGGTCAGCTCGCCCGTGACCATTTCCAGCAACCCGACCAAGGCATTGCGGACACTGTCACCAGGCTCGATTTCCAGCAGTTGGGCGGCATCGGCCAACCAggcatcggc                                                                                                                                                 <  8:356227/143‑1 (MQ=255)
        tcGGTAGGCAGCAGCAGGACGATCGCCATGTCGTCGCTGGTCAGCTCGCCCTTGACCATTTCCTGCAAGCCGACCAAGGCATTGCGGACACTGTCACCAGGCTCGATTTCCAGCAGTTGGGCGGCATCGGCCAACCAggcatcggcatc                                                                                                                                              <  7:103627/149‑1 (MQ=255)
                cagcagcagGACGATCGCCATGTCGTCGCTGGTCAGCTCGCCCTTGACCATTTCCTGCAAGCCGACCAAGGCATTGCGGACACTGTCACCAGGCTCGATTTCCAGCAGTTGGGCGGCATCGGCCAACCAGGCATCGGCATCGAAgccgg                                                                                                                                      >  1:68461/1‑149 (MQ=255)
                cagcagcagGACGATCGCCATGTCGTCGCTGGTCAGCTCGCCCTTGACCATTTCCTGCAAGCCGACCAAGGCATTGCGGACACTGTCACCAGGCTCGATTTCCAGCAGTTGGGCGGCATCGGCCAACCAGGCATCGGCATCGAAgccgg                                                                                                                                      <  4:359468/149‑1 (MQ=255)
                 agcagcagGACGATCGCCATGTCGTCGCTGGTCAGCTCGCCCTTGACCATTTCCTGCAAGCCGACCAAGGCATTGCGGACACTGTCACCAGGCTCGATTTCCAGCAGTTGGGCGGCATCGGCCAACCAGGCATCGGCATCGAAgccggc                                                                                                                                     <  8:238407/149‑1 (MQ=255)
                               cGCCATGTCGTCGCTGGTCAGCTCGCCCTTGACCATTTCCTGCAAGCCGACCAAGGCATTGCGGACACTGTCACCAGGCTCGATTTCCAGCAGTTGGGCGGCATCGGCCAACCAGGCATCGGCATCGAAGCCGGCGCCGGCGCAGCTGc                                                                                                                       >  6:129755/1‑149 (MQ=255)
                                      tcgtcgCTGGTCAGCTCGCCCTTGACCATTTCCTGCAAGCCGACCAAGGCATTGCGGACACTGTCACCAGGCTCGATTTCCAGCAGTTGGGCGGCATCGGCCAACCAGGCATCGGCATCGAAGCCGGCGCCGGCGCAGCTGCGGCCaa                                                                                                                 <  4:321806/148‑1 (MQ=255)
                                           gctgctCAGATCGACCTTGACCATTTCCTGCAAGCCGACCAAAGAATTGCAGACACTGTCACAAGGCTCGATTTCAAGCAGTTGGGCGGCAAAGGCCAAACAGGCATAGGAATCGAAgtcggcgactgc                                                                                                                               >  4:265358/1‑124 (MQ=255)
                                           gCTGGTCAGCTCGCCCTTGACCATTTCCTGCAAGCCGACCAAGGCATTGCGGACACTGTCACCAGGCTCGATTTCCAGCAGTTGGGCGGCATCGGCCAACCAGGCATCGGCATCGAAgtcggcgccggc                                                                                                                               <  3:265358/129‑1 (MQ=255)
                                           gCTGGTCAGCTCGCCCTTGACCATTTCCTGCAAGCCGACCAAGGCATTGCGGACACTGTCACCAGGCTCGATTTCCAGCAGTTGGGCGGCATCGGCCAACCAGGCATCGGCATCGAAGCCGGCGCCGGCGCAGCTGCGGCCAATcagc                                                                                                            <  5:360518/148‑1 (MQ=255)
                                           gCTGGTCAGCTCGCCCTTGACCATTTCCTGCAAGCCGACCAAGGCATTGCGGACACTGTCACCAGGCTCGATTTCCAGCAGTTGGGCGGCATCGGCCAACCAGGCATCGGCATCGAAGCCGGCGCCGGCGCAGCTGCGGCCAATcag                                                                                                             >  2:21558/1‑147 (MQ=255)
                                           gCTGGTCAGCTCGCCCTTGACCATTTCCTGCAAGCCGACCAAGGCATTGCGGACACTGTCACCAGGCTCGATTTCCAGCAGTTGGGCGGCATCGGCCAACCAGGCATCGGCATCGAAGCCGGCGCCGGCGCAGCTGCGGCCAATcag                                                                                                             >  1:89737/1‑147 (MQ=255)
                                              ggTCAGCTCGCCCTTGACCATTTCCTGCAAGCCGACCAAGGCATTGCGGACACTGTCACCAGGCTCGATTTCCAGCAGTTGGGCGGCGTCGGCCAACAAGGCATCGGCATCGAAGCCGGCGCCGGCGCAGCTGCGGCCAATCAGCAGGc                                                                                                        >  5:55041/1‑149 (MQ=255)
                                                   gCTCGCCCTTGACCATTTCCTGCAAGCCGACCAAGGCATTGCGGACACTGTCACCAGGCTCGATTTCCAGCAGTTGGGCGGCATCGGCCAACCAggcatcggcat                                                                                                                                               <  2:285993/105‑1 (MQ=255)
                                                   gCTCGCCCTTGACCATTTCCTGCAAGCCGACCAAGGCATTGCGGACACTGTCACCAGGCTCGATTTCCAGCAGTTGGGCGGCATCGGCCAACCAggcatcggcat                                                                                                                                               >  1:285993/1‑105 (MQ=255)
                                                    cTCGCCCTTGACCATTTCCTGCAAGCCGACCAAGGCATTGCGGACACTGTCACCAGGCTCGATTTCCAGCAGTTGGGCGGCATCGGCCAACCAGGCATCGGCATCGAAGCCGGCGCCGGCGCAGCTGCGGCCAATCAGCAGGCCGTg                                                                                                    >  6:193518/1‑147 (MQ=255)
                                                           ttGACCATTTCCTGCAAGCCGACCAAGGCATTGCGGACACTGTCACCAGGCTCGATTTCCAGCAGTTGGGCGGCATCGGCCAACCAGGCATCGGCATCGAAGCCGGCGCCGGCGCAGCTGCGGCCAATCAGCAGGCCGTGCAGTTCGGc                                                                                           >  2:160584/1‑149 (MQ=255)
                                                               ccATTTCCTGCAAGCCGACCAAGGCATTGCGGACACTGTCACCAGGCTCGATTTCCAGCAGTTGGGCGGCATCGGCCAACCAGGCATCGGCATCGAAGCCGGCGCCGGCGCAGCTGCGGCCAATCAGCAGGCCGTGCAGTTCGGCCggg                                                                                       <  2:68461/149‑1 (MQ=255)
                                                               ccATTTCCTGCAAGCCGACCAAGGCATTGCGGACACTGTCACCAGGCTCGATTTCCAGCAGTTGGGCGGCATCGGCCAACCAGGCATCGGCATCGAAGCCGGCGCCGGCGCAGCTGCGGCCAATCAGCAGGCCGTGCAGTTCGGCCggg                                                                                       >  7:55744/1‑149 (MQ=255)
                                                                cATTTCCTGCAAGCCGACCAAGGCATTGCGGACACTGTCACCAGGCTCGATTTCCAGCAGTTGGGCGGCATCGGCCAACCAGGCATCGGCATCGAAGCCGGCGCCGGCGCAGCTGCGGCCAATCAGCAGGCCGTGCAGTTCGGCCgggg                                                                                      >  6:222961/1‑149 (MQ=255)
                                                                  tttCCTGCAAGCCGACCAAGGCATTGCGGACACTGTCACCAGGCTCGATTTCCAGCAGTTGGGCGGCATCGGCCAACCAGGCATCGGCATCGAAGCCGGCGCCGGCGCAGCTGTGGCCAATCAGCAGGCCGTGCAGTTCGGCCGGGGt                                                                                     >  7:118961/1‑148 (MQ=255)
                                                                     ccTGCAAGCCGACCAAGGCATTGCGGACACTGTCACCAGGCTCGATTTCCAGCAGTTGGGCGGCATCGGCCAACCAGGCATCGGCATCGAAGCCGGCGCCGGCGCAGCTGCGGCCAATCAGCAGGCCGTGCAGTTCGGCCGGGGTGa                                                                                   >  7:302957/1‑147 (MQ=255)
                                                                      cTGCAAGCCGACCAAGGCATTGCGGACACTGTCACCAGGCTCGATTTCCAGCAGTTGGGCGGCATCGGCCAACCAGGCATCGGCATCGAAGCCGGCGCCGGCGCAGCTGCGGCCAATCAGCAGGCCGTGCAGTTCGGCCGGGGTGACAg                                                                                >  2:263217/1‑149 (MQ=255)
                                                                         cAAGCCGACCAAGGCATTGCGGACACTGTCACCAGGCTCGATTTCCAGCAGTTGGGCGGCATCGGCCAACCAGGCATCGGCATCGAAGCCGGCGCCGGCGCAGCTGCGGCCAATCAGCAGGCCGTGCAGTTCGGCCGGGGTGACAgg                                                                               <  1:21558/147‑1 (MQ=255)
                                                                         cAAGCCGACCAAGGCATTGCGGACACTGTCACCAGGCTCGATTTCCAGCAGTTGGGCGGCATCGGCCAACCAGGCATCGGCATCGAAGCCGGCGCCGGCGCAGCTGCGGCCAATCAGCAGGCCGTGCAGTTCGGCCGGGGTGACAGGAt                                                                             <  2:89737/149‑1 (MQ=255)
                                                                               gACCAAGGCATTGCGGACACTGTCACCAGGCTCGATTTCCAGCAGTTGGGCGGCATCGGCCAACCAGGCGTCGGCATCGAAGCCGGCGCCGGCGCAGCTGCGGCCAATCAGCAGGCCGTGCAGTTCGGCCGGGGTGACAGGATGGCCAt                                                                       >  7:302545/1‑149 (MQ=255)
                                                                               gACCAAGGCATTGCGGACACTGTCACCAGGCTCGATTTCCAGCAGTTGGGCGGCATCGGCCAACCAGGCGTCGGCATCGAAGCCGGCGCCGGCGCAGCTGCGGCCAATCAGCAGGCCGTGCAGTTCGGCCGGGGTGACAGGATGGCCAt                                                                       >  7:307859/1‑149 (MQ=255)
                                                                               gACCAAGGCATTGCGGACACTGTCACCAGGCTCGATTTCCAGCAGTTGGGCGGCATCGGCCAACCAGGCATCGGCATCGAAGCCGGCGCCGGCGCAGCTGCGGCCAATCAGCAGGCCGTGCAGTTCGGCCGGGGTGACAGGATGGCCAt                                                                       >  5:218292/1‑149 (MQ=255)
                                                                                       cATTGCGGACACTGTCACCAGGCTCGATTTCCAGCAGTTGGGCGGCGTCGGCCAACCAGGCATCGGCATCGAAGCCGGCGCCGGCGCAGCTGCGGCCAATCAGCAGGCCGTGCAGTTCGGCCGGGGTGACAGGATGGCCATTGCTCgag                                                               <  6:55041/149‑1 (MQ=255)
                                                                                              gACACTGTCACCAGGCTCGATTTCCAGCAGTTGGGCGGCATCGGCCAACCAGGCATCGGCATCGAAGCCGGCGCCGGCGCAGCTGCGGCCAATCAGCAGGCCGTGCAGTTCGGCCGGGGTGACAGGATGGCCATTGCTCGAG‑Agcagca                                                       <  5:193518/149‑1 (MQ=255)
                                                                                                   tGTCACCAGGCTCGATTTCCAGCAGTTGGGCGGCATCGGCCAACCAGGCATCGGCATCGAAGCCGGCGCCGGCGCa                                                                                                                            <  3:201532/76‑1 (MQ=255)
                                                                                                       aCCAGGCTCGATTTCCAGCAGTTGGGCGGCATCGGCCAACCAGGCATCGGCATCGAAGCCGGCGCCGGCGCAGCTGCGGCCAATCAGCAGGCCGTGCAGTTCGGCCGGGGTGACAGGATGGCCATTGCTCGAG‑AGCAGCATGGCGAAAg                                              >  7:320877/1‑149 (MQ=255)
                                                                                                        ccAGGCTCGATTTCCAGCAGTTGGGCGGCATCGGCCAACCAGGCATCGGCATCGAAGCCGGCGCCGGCGCAGCTGCGGCCAATCAGCAGGCCGTGCAGTTCGGCCGGGGTGACAGGATGGACATTGCTCGAG‑AGCAGCATGTCGAAAGc                                             >  4:223570/1‑149 (MQ=255)
                                                                                                          aGGCTCGATTTCCAGCAGTTGGGCGGCATCGGCCAACCAGGCATCGGCATCGAAGCCGGCGCCGGCGCAGCTGGGGCCAATCAGCAGGCCGTGCAGTTCGGCCGGGGTGACAGGATGGCCATTGCTCGAG‑AGCAGCATGGCGAAAGCGt                                           <  8:118961/149‑1 (MQ=255)
                                                                                                          aGGCTCGATTTCCAGCAGTTGGGCGGCATCGGCCAACCAGGCATCGGCATCGAAGCCGGCGCCGGCGCAGCTGCGGCCAATCAGCAGGCCGTGCAGTTCGGCCGCGGAGACAGGATGGCCATTGCTCGAG‑AGCAGCATGGCGAAAGCGt                                           >  5:173782/1‑149 (MQ=255)
                                                                                                           ggCTCGATTTCCAGCAGTTGGGCGGCATCGGCCAACCAGGCATCGGCATCGAAGCCGGCGCCGGCGCAGCTGCGGCCAATCAGCAGGCCGTGCAGTTCGGCCGGGGTGACAGGATGGCCATTGCTCGAG‑AGCAGCATGGCGAAAGCGtt                                          >  3:59582/1‑149 (MQ=255)
                                                                                                             cTCGATTTCCAGCAGTTGGGCGGCATCGGCCAACCAGGCGTCGGCATCGAAGCCGGCGCCGGCGCAGCTGCGGCCAATCAGCAGGCCGTGCAGTTCGGCCGGGGTGACAGGATGGCCATTGCTCGAG‑AGCAGCATGGCGAAAGCGTTGt                                        <  8:307859/149‑1 (MQ=255)
                                                                                                             cTCGATTTCCAGCAGTTGGGCGGCATCGGCCAACCAGGCGTCGGCATCGAAGCCGGCGCCGGCGCAGCTGCGGCCAATCAGCAGGCCGTGCAGTTCGGCCGGGGTGACAGGATGGCCATTGCTCGAG‑AGCAGCATGGCGAAAGCGTTGt                                        <  8:302545/149‑1 (MQ=255)
                                                                                                              tCGATTTCCAGCAGTTGGGCGGCATCGGCCAACCAGGCATCGGCATCGAAGCCGGCGCCGGCGCAGCTGCGGCCAATcagc                                                                                                            <  5:235426/81‑1 (MQ=255)
                                                                                                              tCGATTTCCAGCAGTTGGGCGGCATCGGCCAACCAGGCATCGGCATCGAAGCCGGCGCCGGCGCAGCTGCGGCCAATcagc                                                                                                            >  6:235426/1‑81 (MQ=255)
                                                                                                              tCGATTTCCAGCAGTTGGGCGGCATCGGCCAACCAGGCATCGGCATCGAAGCCGGCGCCGGCGCAGCTGCGGCCAATCAGCAGGCCGTGCAGTTCGGCCGGGGTGACAGGATGGCCATTGCTCGAG‑AGCAGCATGGCGAAAGCGTTg                                         >  3:124155/1‑147 (MQ=255)
                                                                                                                gATTTCCAGGAGTTGGGGGGCATGGGCCAACCAGGCATCGGCATCGAAGCCGGCGCCGGCGCAGCTGCGGCCAATCAGCAGGCCGTGCAGTTCGGCCGGGGTGACAGGATGGCGATTGCTCGAG‑AGCAGCATGGCGAAAGCGTTGTa                                       <  6:173782/147‑1 (MQ=255)
                                                                                                                  tttCCAGCAGTTGGGCGGCATCGGCCAACCAGGCATCGGCATCGAAGCCGGCGCCGGCGCAGCTGCGGCCAATCAGCAGGCCGTGCAGTTCGGCCGGGGTGACAGGATGGCCATTGCTCGAG‑AGCAGCATGGCGAAAGCGTTGTAGGGc                                   <  6:121698/149‑1 (MQ=255)
                                                                                                                  tttCCAGCAGTTGGGCGGCATCGGCCAACCAGGCATCGGCATCGAAGCCGGCGCCGGCGCAGCTGCGGCCAATCAGCAGGCCGTGCAGTTCGGCCGGGGTGACAGGATGGCCATTGCTCGAG‑AGCAGCATGGCGAAAGCGTTGTAGGGc                                   <  5:222961/149‑1 (MQ=255)
                                                                                                                      cagcagTTGGGCGGCATCGGCCAACCAGGCATCGGCATCGAAGCCGGCGCCGGCGCAGCTGCGGCCAATCAGCAGGCCGTGCAGTTCGGCCGGGGTGACAGGATGGCCATTGCTCGAG‑AGCAGCATGGCGAAAGCGTTGTAGGGCGAtt                               >  8:262868/1‑149 (MQ=255)
                                                                                                                      cagcagTTGGGCGGCATCGGCCAACCAGGCATCGGCATCGAAGCCGGCGCCGGCGCAGCTGCGGCCAATCAGCAGGCCGTGCAGTTCGGCCGGGGTGACAGGATGGCCATTGCTCGAG‑AGCAGCATGGCGAAAGCGTTGTAGGGCGAtt                               >  2:246822/1‑149 (MQ=255)
                                                                                                                      cagcagTTGGGCGGCATCGGCCAACCAGGCATCGGCATCGAAGCCGGCGCCGGCGCAGCTGCGGCCAATCAGCAGGCCGTGCAGTTCGGCCGGGGTGACAGGATGGCCATTGCTCGAG‑AGCAGCATGGCGAAAGCGTTGTAGGGCGAtt                               <  8:320877/149‑1 (MQ=255)
                                                                                                                        gcagTTGGGCGGCATCGGCCAACCAGGCATCGGCATCGAAGCCGGCGCCGGCGCAGCTGCGGCCAATCAGCAGGCCGTGCAGTTCGGCCGGGGTGACAGGATGGCCATTGCTCGAG‑AGCAGCATGGCGAAAGCGTTGTAGGGCGATTGa                             <  1:160584/149‑1 (MQ=255)
                                                                                                                        gcagTTGGGCGGCATCGGCCAACCAGGCATCGGCATCGAAGCCGGCGCCGGCGCAGCTGCGGCCAATCAGCAGGCCGTGCAGTTCGGCCGGGGTGACAGGATGGCCATTGCTCGAG‑AGCAGCATGGCGAAAGCGTTGTAGGGCGATTGa                             <  4:124155/149‑1 (MQ=255)
                                                                                                                         cagTTGGGCGGCATCGGCCAACCAGGCATCGGCATCGAAGCCGGCGCCGGCGCAGCTGCGGCCAATCAGCAGGCCGTGCAGTTCGGCCGGGGTGACAGGATGGCCAt                                                                       <  6:236893/107‑1 (MQ=255)
                                                                                                                         cagTTGGGCGGCATCGGCCAACCAGGCATCGGCATCGAAGCCGGCGCCGGCGCAGCTGCGGCCAATCAGCAGGCCGTGCAGTTCGGCCGGGGTGACAGGATGGCCAt                                                                       >  5:236893/1‑107 (MQ=255)
                                                                                                                            ttGGGCGGCATCGGCCAACCAGGCATCGGCATCGAAGCCGGCGCCGGCGCAGCTGCGGCCAATCAGCAGGCCGTGCAGTTCGGCCGGGGTGACAGGATGGCCATTGCTCGAG‑AGCAGCATGGCGaaa                                               <  7:223430/127‑1 (MQ=255)
                                                                                                                            ttGGGCGGCATCGGCCAACCAGGCATCGGCATCGAAGCCGGCGCCGGCGCAGCTGCGGCCAATCAGCAGGCCGTGCAGTTCGGCCGGGGTGACAGGATGGCCATTGCTCGAG‑AGCAGCATGGCGaaa                                               >  8:223430/1‑127 (MQ=255)
                                                                                                                               ggcggcATCGGCCAACCAGGCATCGGCATCGAAGCCGGCGCCGGCGCAGCTGCGGCCAATCAGCAGGCCGTGCAGTTCGGCCGGGGTGACAGGATGGCCATTGCTCGAG‑AGCAGCATGGCGAAAGCGTTGTAGGGCGATTGAGTATtgg                      <  1:246822/149‑1 (MQ=255)
                                                                                                                               ggcggcATCGGCCAACCAGGCATCGGCATCGAAGCCGGCGCCGGCGCAGCTGCGGCCAATCAGCAGGCCGTGCAGTTCGGCCGGGGTGACAGGATGGCCATTGCTCGAG‑AGCAGCATGGCGAAAGCGTTGTAGGGCGATTGAGTATtg                       <  8:302957/148‑1 (MQ=255)
                                                                                                                                   gcATCGGCCAACCAGGCATCGGCATCGAAGCCGGCGCCGGCGCAGCTGCGGCCAATCAGCAGGCCGTGCAGTTCGGCCGGGGTGACAGGATGGCCATTGCTCGAG‑AGCAGCATGGCGAAAGCGTTGTAGGGCGATTGAGTATtgggcat                  <  6:218292/149‑1 (MQ=255)
                                                                                                                                    cATCGGCCAACCAGGCATCGGCATCGAAGCCGGCGCCGGCGCAGCTGCGGCCAATCAGCAGGCCGTGCAGTTCGGCCGGGGTGACAGGATGGCCATTGCTCGAG‑AGCAGCATGGCGAAAGCGTTGTAGGGCGATTGAGTATtgggcatg                 <  8:202117/149‑1 (MQ=255)
                                                                                                                                     aTCGGCCAACCAGGCATCGGCATCGAAGCCGGCGCCGGCGCAGCTGCGGCCAATCAGCAGGCCGTGCAGTTCGGCCGGGGTGACAGGATGGCCATTGCTCGAGTAGCAGCATGGCGAAAGCGTTGTAGGGCGATTGAGTATtgggcatg                 <  1:263217/149‑1 (MQ=255)
                                                                                                                                         gCCAACCAGGCATCGGCATCGAAGCCGGCGCCGGCGCAGCTGCGGCCAATCAGCAGGCCGTGCAGTTCGGCCGGGGTGACAGGATGGCCATTGCTCGAG‑AGCAGCATGGCGAAAGCGTTGTAGGGCGATTGAGTATTGGGCATGGGCAg            >  3:143767/1‑149 (MQ=255)
                                                                                                                                            aaCCAGGCATCGGCATCGAAGCCGGCGCCGGCGCAGCTGCGGCCAATCAGCAGGCCGTGCAGTTAGGCCGGGGTGACAGGATGGCCATTGCTAGAG‑AGCAGCATGGCGAAAGCGTTGTAGGGGGATTGAGTATTGGGCATGGGCAGc           >  1:273239/1‑147 (MQ=255)
                                                                                                                                                aGGCATCGGCATCGAAGCCGGCGCCGGCGCAGCTGCGGCCAATCAGCAGGCCGTGCAGTTCGGCCGGGGTGACAGGATGGCCATTGCTCGAG‑AGCAGCATGGCGAAAGCGTTGTAGGGCGATTGAGTATTGGGCATGGGCAGCTAGgcg     >  5:167297/1‑149 (MQ=255)
                                                                                                                                                 ggcatcggcatcGAAGCCGGCGCCGGCGCAGCTGCGGCCAATCAGCAGGCCGTGCAGTTCGGCCGGGGTGACAGGATGGCCATTGCTCGAG‑AGCAGCATGGCGAAAGCGTTGTAGGGCGATTGAGTATTGGGCATGGGCAGCTAGgcgc    <  4:59582/149‑1 (MQ=255)
                                                                                                                                                   catcggcatcgAAGCCGGCGCCGGCGCAGCTGCGGCCAATCAGCAGGCCGTGCAGTTCGGCCGGGGTGACAGGATGGCCATTGCTCGAG‑AGCAGCATGGCGAAAGCGTTGTAGGGCGATTGAGTATTGGGCATGGGCAGCTAGGCGCCa  <  6:167297/149‑1 (MQ=255)
                                                                                                                                                    atcggcatcgAAGCCGGCGCCGGCGCAGCTGCGGCCAATCAGCAGGCCGTGCAGTTCGGCCGGGGTGACAGGATGGCCATTGCTCGAG‑AGCAGCATGGCGAAAGCGTTGTAGGGCGATTGAGTATTGGGCATGGGCAGCTAGgcgc    <  8:28970/146‑1 (MQ=255)
                                                                                                                                                    atcggcatcgAAGCCGGCGCCGGCGCAGCTGCGGCCAATCAGCAGGCCGTGCAGTTCGGCCGGGGTGACAGGATGGCCATTGCTCGAG‑AGCAGCATGGCGAAAGCGTTGTAGGGCGATTGAGTATTGGGCATGGGCAGCTAGgcgc    >  7:28970/1‑146 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CAGCATCATCGGTAGGCAGCAGCAGGACGATCGCCATGTCGTCGCTGGTCAGCTCGCCCTTGACCATTTCCTGCAAGCCGACCAAGGCATTGCGGACACTGTCACCAGGCTCGATTTCCAGCAGTTGGGCGGCATCGGCCAACCAGGCATCGGCATCGAAGCCGGCGCCGGCGCAGCTGCGGCCAATCAGCAGGCCGTGCAGTTCGGCCGGGGTGACAGGATGGCCATTGCTCGAG‑AGCAGCATGGCGAAAGCGTTGTAGGGCGATTGAGTATTGGGCATGGGCAGCTAGGCGCCA  >  NC_002947/5933697‑5933992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑