Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,966,323 A→T 5.0% intergenic (‑392/+38) PP_5232 ← / ← amtB hypothetical protein/ammonium transporter AmtB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,966,3230AT 5.0% 307.3 / 3.4 99intergenic (‑392/+38)PP_5232/amtBhypothetical protein/ammonium transporter AmtB
Reads supporting (aligned to +/- strand):  ref base A (41/53);  new base T (2/3);  total (43/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.78e-01

CAAACACCCTTCGGCGCGCATTCTAGCGCGCCCGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGACCCGCAGGCCCATCACCACATCCAGCACCTTGAG  >  NC_002947/5966175‑5966462
                                                                                                                                                     |                                                                                                                                           
cAAACACCCTTCGGCGCGCATTCTAGCGCGCCCGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCaaaaaaa                                                                                                                                             >  2:257668/1‑149 (MQ=255)
  aaCACCCTTCGGCGCGCATTCTAGCGCGCCCGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATg                                                                                                                                           >  6:76236/1‑149 (MQ=255)
      cccTTCGGCGCGCATTCTAGCGCGCCCGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCg                                                                                                                                       <  1:257668/149‑1 (MQ=255)
       ccTTCGGCGCGCATTCTAGCGCGCCCGTGGAAAAAGCAAAGCACCTTAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCgg                                                                                                                                      >  1:48422/1‑149 (MQ=255)
       ccTTCGGCGCGCATTCTAGCGCGCCCGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCgg                                                                                                                                      >  6:306111/1‑149 (MQ=255)
             gcgcgcATTCTAGCGCGCCCGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGcc                                                                                                                                >  7:84810/1‑149 (MQ=255)
               gcgcATTCTAGCGCGCCCGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAACTTGCCCGGCTTGCCgg                                                                                                                              >  1:83138/1‑149 (MQ=255)
                cgcATTCTAGCGCGCCCGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCggg                                                                                                                             <  3:249662/149‑1 (MQ=255)
                   aTTCTAGCGCGCCCGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCaa                                                                                                                          <  5:334963/149‑1 (MQ=255)
                     tCTAGCGCGCCCGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGt                                                                                                                        >  3:230984/1‑149 (MQ=255)
                      cTAGCGCGCCCGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGt                                                                                                                        <  4:354991/148‑1 (MQ=255)
                       tAGCGCGCCCGTGGAAAAAGCAAATCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGtt                                                                                                                       <  3:189440/148‑1 (MQ=255)
                       tAGCGCGCCCGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAACTTGCCCGGCTTGCCGGGCattttt                                                                                                                      >  1:315175/1‑144 (MQ=255)
                       tAGCGCGCCCGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACATGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGttt                                                                                                                      <  3:237633/149‑1 (MQ=255)
                         gcgcgcCCGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGttttt                                                                                                                    <  4:220115/149‑1 (MQ=255)
                         gcgcgcCCGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGtttt                                                                                                                     >  7:341425/1‑148 (MQ=255)
                         gcgcgcCCGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGttt                                                                                                                      <  8:84810/147‑1 (MQ=255)
                         gcgcgcCCGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGtt                                                                                                                       <  2:48422/146‑1 (MQ=255)
                         gcgcgcCCGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATCCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGttttt                                                                                                                    <  1:260074/149‑1 (MQ=255)
                             gcCCGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAACTTGCCCGGCTTGCCGGGCATTTTTTTGcca                                                                                                                <  2:315175/149‑2 (MQ=38)
                             gcCCGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAACTTGCCCGGCTTGCCGGGCATTTTTTTGcca                                                                                                                <  2:83138/149‑2 (MQ=38)
                              cccGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAACTTGCCCGGCTTGCCGGGCATTTTTTTGccag                                                                                                               <  3:24911/149‑3 (MQ=38)
                                     aaaaaGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGGTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCACGTTTTTAGCGCGTCGCAg                                                                                                        >  8:273841/1‑149 (MQ=255)
                                     aaaaaGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAg                                                                                                        >  2:43648/1‑149 (MQ=255)
                                     aaaaaGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAg                                                                                                        >  1:239428/1‑149 (MQ=255)
                                      aaaaGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGt                                                                                                       <  5:306111/149‑1 (MQ=255)
                                        aagcaaagcaCCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTa                                                                                                     <  4:237890/149‑1 (MQ=255)
                                        aagcaaagcaCCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTa                                                                                                     >  6:99542/1‑149 (MQ=255)
                                         agcaaagcaCCATTAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCTTGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTAc                                                                                                    <  2:143174/149‑1 (MQ=255)
                                         agcaaagcaCCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTa                                                                                                     >  3:17176/1‑148 (MQ=255)
                                         agcaaagcaCCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTa                                                                                                     >  1:241044/1‑148 (MQ=255)
                                            aaagcaCCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAgg                                                                                                 >  3:287221/1‑149 (MQ=255)
                                            aaagcaCCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAgg                                                                                                 >  6:243543/1‑149 (MQ=255)
                                            aaagcaCCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAgg                                                                                                 <  2:129478/149‑1 (MQ=255)
                                             aagcaCCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGt                                                                                                >  8:264042/1‑149 (MQ=255)
                                              agcaCCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCa                                                                                                         >  8:16970/1‑139 (MQ=255)
                                              agcaCCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCa                                                                                                         <  7:16970/139‑1 (MQ=255)
                                              agcaCCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGtt                                                                                               <  7:192003/149‑1 (MQ=255)
                                               gcaCCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGccc                                                                                                                                        <  7:109370/107‑1 (MQ=255)
                                               gcaCCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGccc                                                                                                                                        >  8:109370/1‑107 (MQ=255)
                                                caCCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTg                                                                                              >  8:123983/1‑148 (MQ=255)
                                                caCCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTg                                                                                              >  4:302528/1‑148 (MQ=255)
                                                caCCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGt                                                                                             <  8:341425/149‑1 (MQ=255)
                                                   cATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGc                                                                                          >  1:20370/1‑149 (MQ=255)
                                                        aCGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAg                                                                                                  >  1:326138/1‑136 (MQ=255)
                                                        aCGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAg                                                                                                  <  2:326138/136‑1 (MQ=255)
                                                        aCGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCgcgc                                                                                     >  3:141261/1‑149 (MQ=255)
                                                        aCGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCgcgc                                                                                     <  3:5505/149‑1 (MQ=255)
                                                          gCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTg                                                                                              >  2:317292/1‑138 (MQ=255)
                                                          gCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTc                                                                                   >  5:215391/1‑149 (MQ=255)
                                                           cccTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCg                                                                                  <  4:17176/149‑1 (MQ=255)
                                                             cTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGtt                                                                                <  5:243543/149‑1 (MQ=255)
                                                             cTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGtt                                                                                <  2:241044/149‑1 (MQ=255)
                                                             cTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCCAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGtt                                                                                <  4:65522/149‑1 (MQ=255)
                                                                cGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTtg                                                                               <  1:43648/147‑1 (MQ=255)
                                                                  gCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCt                                                                           >  1:258944/1‑149 (MQ=255)
                                                                  gCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCt                                                                           <  4:287221/149‑1 (MQ=255)
                                                                     tGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCt                                                                           >  1:273519/1‑147 (MQ=255)
                                                                      gTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGa                                                                         <  5:99542/147‑1 (MQ=255)
                                                                      gTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGa                                                                         <  6:215391/147‑1 (MQ=255)
                                                                       tAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCTTGCCGGGCattt                                                                                                                        >  3:212339/1‑96 (MQ=255)
                                                                       tAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCTTGCCGGGCattt                                                                                                                        <  4:212339/99‑4 (MQ=255)
                                                                        aaGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTtgtg                                                                             >  7:289014/1‑141 (MQ=255)
                                                                        aaGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTtgtg                                                                             <  8:289014/141‑1 (MQ=255)
                                                                           ttttttACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTg                                                                                              <  4:54034/121‑1 (MQ=255)
                                                                           ttttttACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTg                                                                                              >  3:54034/1‑121 (MQ=255)
                                                                               ttACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGa                                                               <  2:239428/148‑1 (MQ=255)
                                                                                tACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAg                                                              <  7:123983/148‑1 (MQ=255)
                                                                                 aCCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAgg                                                             <  3:94548/148‑1 (MQ=255)
                                                                                    tACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAg                                                                    <  4:217203/138‑1 (MQ=255)
                                                                                    tACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAg                                                                    >  3:217203/1‑138 (MQ=255)
                                                                                          aCGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCcgaccgac                                                    >  7:303643/1‑148 (MQ=255)
                                                                                             aaCGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACtctt                                                <  2:337261/149‑1 (MQ=255)
                                                                                              aCGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACtcttc                                               <  2:258944/149‑1 (MQ=255)
                                                                                                 tgtCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACtcttcttc                                            >  3:228856/1‑149 (MQ=255)
                                                                                                   tCGAATAAACCGTTTGCC‑GTGCGGGAAATGCGGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGGCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGGCGAGGCCGACCGACTcttcttctt                                          <  2:115785/149‑1 (MQ=255)
                                                                                                     gAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCg                                        <  3:302528/149‑1 (MQ=255)
                                                                                                      aaTAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACtcttc                                               >  7:95669/1‑141 (MQ=255)
                                                                                                      aaTAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACtcttc                                               <  8:95669/141‑1 (MQ=255)
                                                                                                      aaTAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGt                                       <  4:230984/149‑1 (MQ=255)
                                                                                                       aTAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGtt                                      <  1:9324/149‑1 (MQ=255)
                                                                                                        tAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGtt                                                                                <  6:69177/106‑1 (MQ=255)
                                                                                                        tAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGtt                                                                                >  5:69177/1‑106 (MQ=255)
                                                                                                        tAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCttcttcttc                                         <  3:330766/145‑1 (MQ=255)
                                                                                                        tAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCttcttcttc                                         >  4:330766/1‑145 (MQ=255)
                                                                                                          aaCCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGtt                                                                                               >  6:11423/1‑89 (MQ=255)
                                                                                                          aaCCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGtt                                                                                               <  5:11423/89‑1 (MQ=255)
                                                                                                           aCCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGAcc                                  >  7:341222/1‑149 (MQ=255)
                                                                                                           aCCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGAcc                                  >  4:53649/1‑149 (MQ=255)
                                                                                                                 tGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGCCTCTTCTTCTTCGTTGACCCGCAgg                            >  6:101828/1‑149 (MQ=255)
                                                                                                                  gCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGACCCGCAgg                            <  8:341222/148‑1 (MQ=255)
                                                                                                                    c‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGACCCGCAGGccc                         <  5:76236/149‑1 (MQ=255)
                                                                                                                    c‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGACCCGCAGGccc                         <  2:273519/149‑1 (MQ=255)
                                                                                                                       tGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACGGACTCTTCTTCTTCGTTGAGCCGCAGGCGCAt                       <  2:20370/149‑1 (MQ=255)
                                                                                                                         cGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCTCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCTGGTCGAGGCCTACCGACTCTTCTTCTTCGTTGACCCGCAGGCCCATca                     <  5:101828/149‑1 (MQ=255)
                                                                                                                                tGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGACCCGCAGGCCCATCACCACATc              <  7:264042/149‑1 (MQ=255)
                                                                                                                                 gCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGACCCGCAGGCCCATCACCACATc              >  4:66017/1‑148 (MQ=255)
                                                                                                                                  cTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGACCCGCAGGCCCATCACCACATCCa            <  7:27314/149‑1 (MQ=255)
                                                                                                                                           tGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGACCCGCAGGCCCATCACAACATCCAGCAGCtt     >  8:331301/1‑147 (MQ=255)
                                                                                                                                             gCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGACCCGCAGGCCCATCACCACATCCAGCACCTTgag  <  3:66017/148‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                           
CAAACACCCTTCGGCGCGCATTCTAGCGCGCCCGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGACCCGCAGGCCCATCACCACATCCAGCACCTTGAG  >  NC_002947/5966175‑5966462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑