Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,966,332 A→T 7.4% intergenic (‑401/+29) PP_5232 ← / ← amtB hypothetical protein/ammonium transporter AmtB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,966,3320AT 7.4% 286.2 / 8.7 94intergenic (‑401/+29)PP_5232/amtBhypothetical protein/ammonium transporter AmtB
Reads supporting (aligned to +/- strand):  ref base A (36/51);  new base T (3/4);  total (39/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.07e-01

GCGCGCATTCTAGCGCGCCCGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGACCCGCAGGCCCATCACCACATCCAGCACCTTGAGAATCACGTAGGT  >  NC_002947/5966188‑5966474
                                                                                                                                                 |                                                                                                                                              
gcgcgcATTCTAGCGCGCCCGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGcc                                                                                                                                            >  7:84810/1‑149 (MQ=255)
  gcgcATTCTAGCGCGCCCGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAACTTGCCCGGCTTGCCgg                                                                                                                                          >  1:83138/1‑149 (MQ=255)
   cgcATTCTAGCGCGCCCGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCggg                                                                                                                                         <  3:249662/149‑1 (MQ=255)
      aTTCTAGCGCGCCCGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCaa                                                                                                                                      <  5:334963/149‑1 (MQ=255)
        tCTAGCGCGCCCGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGt                                                                                                                                    >  3:230984/1‑149 (MQ=255)
         cTAGCGCGCCCGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGt                                                                                                                                    <  4:354991/148‑1 (MQ=255)
          tAGCGCGCCCGTGGAAAAAGCAAATCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGtt                                                                                                                                   <  3:189440/148‑1 (MQ=255)
          tAGCGCGCCCGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAACTTGCCCGGCTTGCCGGGCattttt                                                                                                                                  >  1:315175/1‑144 (MQ=255)
          tAGCGCGCCCGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACATGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGttt                                                                                                                                  <  3:237633/149‑1 (MQ=255)
            gcgcgcCCGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGttttt                                                                                                                                <  4:220115/149‑1 (MQ=255)
            gcgcgcCCGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGtttt                                                                                                                                 >  7:341425/1‑148 (MQ=255)
            gcgcgcCCGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGttt                                                                                                                                  <  8:84810/147‑1 (MQ=255)
            gcgcgcCCGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGtt                                                                                                                                   <  2:48422/146‑1 (MQ=255)
            gcgcgcCCGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATCCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGttttt                                                                                                                                <  1:260074/149‑1 (MQ=255)
                gcCCGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAACTTGCCCGGCTTGCCGGGCATTTTTTTGcca                                                                                                                            <  2:83138/149‑2 (MQ=38)
                gcCCGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAACTTGCCCGGCTTGCCGGGCATTTTTTTGcca                                                                                                                            <  2:315175/149‑2 (MQ=38)
                 cccGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAACTTGCCCGGCTTGCCGGGCATTTTTTTGccag                                                                                                                           <  3:24911/149‑3 (MQ=38)
                        aaaaaGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGGTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCACGTTTTTAGCGCGTCGCAg                                                                                                                    >  8:273841/1‑149 (MQ=255)
                        aaaaaGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAg                                                                                                                    >  1:239428/1‑149 (MQ=255)
                        aaaaaGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAg                                                                                                                    >  2:43648/1‑149 (MQ=255)
                         aaaaGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGt                                                                                                                   <  5:306111/149‑1 (MQ=255)
                           aagcaaagcaCCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTa                                                                                                                 >  6:99542/1‑149 (MQ=255)
                           aagcaaagcaCCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTa                                                                                                                 <  4:237890/149‑1 (MQ=255)
                            agcaaagcaCCATTAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCTTGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTAc                                                                                                                <  2:143174/149‑1 (MQ=255)
                            agcaaagcaCCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTa                                                                                                                 >  1:241044/1‑148 (MQ=255)
                            agcaaagcaCCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTa                                                                                                                 >  3:17176/1‑148 (MQ=255)
                               aaagcaCCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAgg                                                                                                             >  6:243543/1‑149 (MQ=255)
                               aaagcaCCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAgg                                                                                                             >  3:287221/1‑149 (MQ=255)
                               aaagcaCCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAgg                                                                                                             <  2:129478/149‑1 (MQ=255)
                                aagcaCCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGt                                                                                                            >  8:264042/1‑149 (MQ=255)
                                 agcaCCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCa                                                                                                                     <  7:16970/139‑1 (MQ=255)
                                 agcaCCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCa                                                                                                                     >  8:16970/1‑139 (MQ=255)
                                 agcaCCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGtt                                                                                                           <  7:192003/149‑1 (MQ=255)
                                   caCCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTg                                                                                                          >  4:302528/1‑148 (MQ=255)
                                   caCCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTg                                                                                                          >  8:123983/1‑148 (MQ=255)
                                   caCCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGt                                                                                                         <  8:341425/149‑1 (MQ=255)
                                      cATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGc                                                                                                      >  1:20370/1‑149 (MQ=255)
                                           aCGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAg                                                                                                              <  2:326138/136‑1 (MQ=255)
                                           aCGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAg                                                                                                              >  1:326138/1‑136 (MQ=255)
                                           aCGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCgcgc                                                                                                 <  3:5505/149‑1 (MQ=255)
                                           aCGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCgcgc                                                                                                 >  3:141261/1‑149 (MQ=255)
                                             gCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTg                                                                                                          >  2:317292/1‑138 (MQ=255)
                                             gCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTc                                                                                               >  5:215391/1‑149 (MQ=255)
                                              cccTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCg                                                                                              <  4:17176/149‑1 (MQ=255)
                                                cTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGtt                                                                                            <  2:241044/149‑1 (MQ=255)
                                                cTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGtt                                                                                            <  5:243543/149‑1 (MQ=255)
                                                cTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCCAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGtt                                                                                            <  4:65522/149‑1 (MQ=255)
                                                   cGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTtg                                                                                           <  1:43648/147‑1 (MQ=255)
                                                     gCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCt                                                                                       <  4:287221/149‑1 (MQ=255)
                                                     gCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCt                                                                                       >  1:258944/1‑149 (MQ=255)
                                                        tGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCt                                                                                       >  1:273519/1‑147 (MQ=255)
                                                         gTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGa                                                                                     <  5:99542/147‑1 (MQ=255)
                                                         gTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGa                                                                                     <  6:215391/147‑1 (MQ=255)
                                                          tAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCTTGCCGGGCattt                                                                                                                                    <  4:212339/99‑4 (MQ=255)
                                                          tAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCTTGCCGGGCattt                                                                                                                                    >  3:212339/1‑96 (MQ=255)
                                                           aaGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTtgtg                                                                                         >  7:289014/1‑141 (MQ=255)
                                                           aaGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTtgtg                                                                                         <  8:289014/141‑1 (MQ=255)
                                                              ttttttACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTg                                                                                                          <  4:54034/121‑1 (MQ=255)
                                                              ttttttACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTg                                                                                                          >  3:54034/1‑121 (MQ=255)
                                                                  ttACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGa                                                                           <  2:239428/148‑1 (MQ=255)
                                                                   tACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAg                                                                          <  7:123983/148‑1 (MQ=255)
                                                                    aCCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAgg                                                                         <  3:94548/148‑1 (MQ=255)
                                                                       tACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAg                                                                                >  3:217203/1‑138 (MQ=255)
                                                                       tACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAg                                                                                <  4:217203/138‑1 (MQ=255)
                                                                             aCGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCcgaccgac                                                                >  7:303643/1‑148 (MQ=255)
                                                                                aaCGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACtctt                                                            <  2:337261/149‑1 (MQ=255)
                                                                                 aCGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACtcttc                                                           <  2:258944/149‑1 (MQ=255)
                                                                                    tgtCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACtcttcttc                                                        >  3:228856/1‑149 (MQ=255)
                                                                                      tCGAATAAACCGTTTGCC‑GTGCGGGAAATGCGGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGGCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGGCGAGGCCGACCGACTcttcttctt                                                      <  2:115785/149‑1 (MQ=255)
                                                                                        gAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCg                                                    <  3:302528/149‑1 (MQ=255)
                                                                                         aaTAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACtcttc                                                           >  7:95669/1‑141 (MQ=255)
                                                                                         aaTAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACtcttc                                                           <  8:95669/141‑1 (MQ=255)
                                                                                         aaTAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGt                                                   <  4:230984/149‑1 (MQ=255)
                                                                                          aTAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGtt                                                  <  1:9324/149‑1 (MQ=255)
                                                                                           tAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGtt                                                                                            >  5:69177/1‑106 (MQ=255)
                                                                                           tAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGtt                                                                                            <  6:69177/106‑1 (MQ=255)
                                                                                           tAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCttcttcttc                                                     <  3:330766/145‑1 (MQ=255)
                                                                                           tAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCttcttcttc                                                     >  4:330766/1‑145 (MQ=255)
                                                                                             aaCCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGtt                                                                                                           >  6:11423/1‑89 (MQ=255)
                                                                                             aaCCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGtt                                                                                                           <  5:11423/89‑1 (MQ=255)
                                                                                              aCCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGAcc                                              >  4:53649/1‑149 (MQ=255)
                                                                                              aCCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGAcc                                              >  7:341222/1‑149 (MQ=255)
                                                                                                    tGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGCCTCTTCTTCTTCGTTGACCCGCAgg                                        >  6:101828/1‑149 (MQ=255)
                                                                                                     gCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGACCCGCAgg                                        <  8:341222/148‑1 (MQ=255)
                                                                                                       c‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGACCCGCAGGccc                                     <  5:76236/149‑1 (MQ=255)
                                                                                                       c‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGACCCGCAGGccc                                     <  2:273519/149‑1 (MQ=255)
                                                                                                          tGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACGGACTCTTCTTCTTCGTTGAGCCGCAGGCGCAt                                   <  2:20370/149‑1 (MQ=255)
                                                                                                            cGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCTCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCTGGTCGAGGCCTACCGACTCTTCTTCTTCGTTGACCCGCAGGCCCATca                                 <  5:101828/149‑1 (MQ=255)
                                                                                                                   tGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGACCCGCAGGCCCATCACCACATc                          <  7:264042/149‑1 (MQ=255)
                                                                                                                    gCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGACCCGCAGGCCCATCACCACATc                          >  4:66017/1‑148 (MQ=255)
                                                                                                                     cTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGACCCGCAGGCCCATCACCACATCCa                        <  7:27314/149‑1 (MQ=255)
                                                                                                                              tGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGACCCGCAGGCCCATCACAACATCCAGCAGCtt                 >  8:331301/1‑147 (MQ=255)
                                                                                                                                gCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGACCCGCAGGCCCATCACCACATCCAGCACCTTgag              <  3:66017/148‑1 (MQ=255)
                                                                                                                                           cccGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGACCCGCAGGCCCATCACCACATCCAGCACCTTGAGAATCACGTAGGt  <  4:141261/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                              
GCGCGCATTCTAGCGCGCCCGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCC‑GTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGACCCGCAGGCCCATCACCACATCCAGCACCTTGAGAATCACGTAGGT  >  NC_002947/5966188‑5966474

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑