Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,986,841	T→A	100%	intergenic (+121/‑78)	PP_5245 → / → kefB‑III	AraC family transcriptional regulator/glutathione‑regulated potassium/H+ antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,986,841	0	T	A	100.0%	153.4 / NA	46	intergenic (+121/‑78)	PP_5245/kefB‑III	AraC family transcriptional regulator/glutathione‑regulated potassium/H+ antiporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (23/23);  total (23/23)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GGTGGCGGTGAACAGTCCGATTAGCGCTGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGAC‑AATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGCTTGCCGCCGTCCTCGCCG  >  NC_002947/5986697‑5986982                                                                                                                                                  |                                                                                                                                              ggtggCGGTGAACAGTCCGATTAGCGCTGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACTAATTTTTCTTCCCGTCAGATGATTGACt                                                                                                                                             <  1:21073/148‑1 (MQ=255) ggtggCGGTGAACAGTCCGATTAGCGCTGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGAC‑AATTTTTCTTCCCGTCAGATGATTGACTGa                                                                                                                                           >  7:167499/1‑149 (MQ=255)        gTGAACAGTCCGATTAGCGCTGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGAC‑AATTTTTCTTCCCGTCAGATGATTGACTGACGCcaca                                                                                                                                    >  1:160817/1‑149 (MQ=255)        gTGAACAGTCCGATTAGCGCTGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGAC‑AATTTTTCTTCCCGTCAGATGATTGACTGACGCcaca                                                                                                                                    >  1:104038/1‑149 (MQ=255)                  cGATTAGCGCTGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGAC‑AATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCa                                                                                                                          <  2:78531/149‑1 (MQ=255)                  cGATTAGCGCTGCCTTCTTCGCGGGCGAGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGAC‑AATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCt                                                                                                                            >  8:320148/1‑147 (MQ=255)                    aTTAGCGCTGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGAC‑AATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACACCTCAg                                                                                                                         >  8:138329/1‑148 (MQ=255)                     ttAGCGCTGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGAC‑AATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGaa                                                                                                                       <  2:96201/149‑1 (MQ=255)                        gcgcTGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGAC‑AATTTTTCTTCCCGTCAGATGATTGACTGACGCCCCACTACCCCTCAGAATTg                                                                                                                    >  3:54249/1‑149 (MQ=255)                          gcTGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGCGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGAC‑AATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTc                                                                                                                  <  2:213390/149‑1 (MQ=255)                                  cttcGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGAC‑AATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCaaa                                                                                                           <  8:167499/148‑1 (MQ=255)                                   ttcGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGAC‑AATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCa                                                                                                                          <  2:84876/132‑1 (MQ=255)                                   ttcGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGAC‑AATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCa                                                                                                                          >  1:84876/1‑132 (MQ=255)                                      gcgGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGAC‑AATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGtt                                                                                                      <  2:81985/149‑1 (MQ=255)                                            gcgcCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGAC‑AATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACg                                                                                                <  2:104038/149‑1 (MQ=255)                                               cccGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGAC‑AATTTTTCTTTCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTg                                                                                             >  3:168402/1‑149 (MQ=255)                                                              aTAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGAC‑AATTTTTCTTCCCTTCAGATGATTGACTGACGCCACACTTCCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATg                                                                              <  7:309733/149‑1 (MQ=255)                                                               tAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGAC‑AATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATg                                                                              <  7:320148/148‑1 (MQ=255)                                                                aGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGAC‑AATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGa                                                                            <  4:256100/149‑1 (MQ=255)                                                                      ggCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGAC‑AATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACACCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGa                                                                      <  7:138329/149‑1 (MQ=255)                                                                         cTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGAC‑AATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGa                                                                            <  5:263351/140‑1 (MQ=255)                                                                         cTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGAC‑AATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGa                                                                            >  6:263351/1‑140 (MQ=255)                                                                                ccAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGAC‑AATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCaaa                                                                                                           >  5:345740/1‑102 (MQ=255)                                                                                ccAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGAC‑AATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCaaa                                                                                                           <  6:345740/102‑1 (MQ=255)                                                                                   ggTAAGCGCCGGCACCGATTGTCACTCTGGACAGGAC‑AATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCacac                                                         <  3:69902/149‑1 (MQ=255)                                                                                        gcgcCGGCACCGATTGTCACTCTGGACAGGAC‑AATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAgg                                                    <  2:160817/149‑1 (MQ=255)                                                                                              gCACCGATTGTCACTCTGGACAGGAC‑AATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCgg                                                                                       <  8:122471/108‑1 (MQ=255)                                                                                              gCACCGATTGTCACTCTGGACAGGAC‑AATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCgg                                                                                       >  7:122471/1‑108 (MQ=255)                                                                                                aCCGATTGTCACTCTGGACAGGAC‑AATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTc                                            <  2:94230/149‑1 (MQ=255)                                                                                                            tctGGACAGGAC‑AATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCt                                           <  8:175816/138‑1 (MQ=255)                                                                                                            tctGGACAGGAC‑AATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCt                                           >  7:175816/1‑138 (MQ=255)                                                                                                                gacaggac‑aATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATg                                                                              >  8:86857/1‑99 (MQ=255)                                                                                                                gacaggac‑aATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATg                                                                              <  7:86857/99‑1 (MQ=255)                                                                                                                gacaggac‑aATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGAt                            >  5:163892/1‑149 (MQ=255)                                                                                                                   aggac‑aATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCt                                                                                                                            >  3:17559/1‑50 (MQ=255)                                                                                                                   aggac‑aATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCt                                                                                                                            <  4:17559/50‑1 (MQ=255)                                                                                                                     gac‑aATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTc                        >  3:173666/1‑148 (MQ=255)                                                                                                                     gac‑aATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTc                        <  4:173666/148‑1 (MQ=255)                                                                                                                         aaTTTTTCTTCCCGTCAGATGATTGACTGCCGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGc                    >  2:367024/1‑149 (MQ=255)                                                                                                                          aTTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGCt                   >  2:329106/1‑149 (MQ=255)                                                                                                                           tttttCTTCCCGTCAGATGATTGACTGACGCCACACTACCACTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGCtt                  >  5:149450/1‑149 (MQ=255)                                                                                                                                 ttcCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGCTTgccgcc            >  8:12624/1‑149 (MQ=255)                                                                                                                                   cccGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGCTTGCCGCCGt          >  8:216678/1‑149 (MQ=255)                                                                                                                                         aGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCtt                                             >  3:176655/1‑108 (MQ=255)                                                                                                                                         aGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCtt                                             <  4:176655/108‑1 (MQ=255)                                                                                                                                           atgatTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGCTTGCCGCCGTCCTCGCCg  >  8:57676/1‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                              GGTGGCGGTGAACAGTCCGATTAGCGCTGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGAC‑AATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGCTTGCCGCCGTCCTCGCCG  >  NC_002947/5986697‑5986982

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑