Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,009,241 C→T 7.5% E538K (GAA→AAA)  PP_5263 ← GGDEF domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,009,2410CT 7.5% 157.7 / 2.4 53E538K (GAA→AAA) PP_5263GGDEF domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base C (29/20);  new base T (2/2);  total (31/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

GCTCCCACAGGATTGCGCGGTCCCTGTGGGAGCGGGCGTGCCCGCGAAGAGGCCCTAAAGGACACCCTCCAACTGGCGGCCAGGCGCCTGCAGCAAATACGCCATCAATTCCCCTATCGGTAGCGGTCTGCTGACCAGGAAGCCTTGCACCTGGTCGCAGCCAAACCCGCGCAACAACGTCAGCTGCTCTTCACTCTCCACACCCTCGGCCACCACCTCGAGGTTGAGGTTGTGCGCCAGGTTGATCATGGCG  >  NC_002947/6009131‑6009383
                                                                                                              |                                                                                                                                              
gCTCCCACAGGATTGCGCGGTCCCTGTGGGAGCGGGCGTGCCCGCGAAGAGGCCCTAAAGGACACCCTCCAACTGGCGGCCAGGCGCCTGCAGCAAATACGCCATCAATTCCCCTATCGGTAGCGGTCTGCTGACCAGGAAGCCTTGCa                                                                                                          >  6:49148/1‑149 (MQ=255)
 cTCCCACAGGATTGCGCGGTCCCTGTGGGAGCGGGCGTGCCCGCGAAGAGGCCCTAAAGGACACCCTCCAACTGGCGGCCAGGCGCCTGCAGCAAATACGCCATCAATTCCCCTATCGGTAGCGGTCTGCTGACCAGGAAGCCTTGCAc                                                                                                         <  5:49148/149‑1 (MQ=255)
    ccACAGGATTGCGCGGTCCCTGTGGGAGCGGGCGTGCCCGCGAAGAGGCCCTAAAGGACACCCTCCAACTGGCGGCCAGGCGCCTGCAGCAAATACGCCATCAATTCCCCTATCGGTAGCGGTCTGCTGACCAGGAAGCCTTGCACCTg                                                                                                      >  6:337679/1‑149 (MQ=255)
     cacaGGATTGCGCGGTCCCTGTGGGAGCGGGCGTGCCCGCGAAGAGGCCCTAAAGGACACCCTCCAACTGGCGGCCAGGCGCCTGCAGCAAATACGCCATCAATTCCCCTATCGGTAGCGGTCTGCTGACCAGGAAGCCTTGCACCTgg                                                                                                     >  7:274264/1‑149 (MQ=255)
               cgcgGTCCCTGTGGGAGCGGGCGTGCCCGCGAAGAGGCCCTAAAGGACACCCTCCAACTGGCGGCCAGGCGCCTGCAGCAAATACGCCATCAATTCCCCTATCGGTAGCGGTCTGCTGACCAGGAAGCCTTGCACCTGGTCGCAGCCa                                                                                            >  6:343743/1‑148 (MQ=255)
                  ggTCCCTGTGGGAGCGGGCGTGCCCGCGAAGAGGCCCTAAAGGACACCCTCCAACTGGCGGCCAGGCGCCTGCAGCAAATACGCCATCAATTCCCCTATCGGTAGCGGTCTGCTGACCAGGAAGCCTTGCACCTGGTCGCAGCCAAAcc                                                                                        >  1:19057/1‑149 (MQ=255)
                       cTGTGGGAGCGGGCGTGCCCGCGAAGAGGCCCTAAAGGACACCCTCCAACTGGCGGCCAGGCGCCTGCAGCAAATACGCCATCAATTCCCCTATCGGTAGCGGTCTGCTGACCAGGAAGCCTTGCACCTGGTCGCAGCCAAACCcgcg                                                                                    >  7:31090/1‑148 (MQ=255)
                       cTGTGGGAGCGGGCGTGCCCGCGAAGAGGCCCTAAAGGACACCCTCCAACTGGCGGCCAGGCGCCTGCAGCAAATACGCCATCAATTCCCCTATCGGTAGCGGTCTGCTGACCAGGAAGCCTTGCACCTGGTCGCAGCCAAACCcgcg                                                                                    >  7:25370/1‑148 (MQ=255)
                       cTGTGGGAGCGGGCGTGCCCGCGAAGAGGCCCTAAAGGACACCCTCCAACTGGCGGCCAGGCGCCTGCAGCAAATACGCCATCAATTCCCCTATCGGTAGCGGTCTGCTGACCAGGAAGCCTTGCACCTGGTCGCAGCCAAACCCgcgc                                                                                   <  5:343743/149‑1 (MQ=255)
                          tgGGAGCGGGCGTGCCCGCGAAGAGGCCCTAAAGGACACCCTCCAACTGGCGGCCAGGCGCCTGCAGCAAATACGCCATCAATTCCCCTATCGGTAGCGGTCTGCTGACCAGGAAGCCTTGCACCTGGTCGCAGCCAAACCCGCGcaa                                                                                 >  4:154433/1‑148 (MQ=255)
                           gggAGCGGGCGTGCCCGCGAAGAGGCCCTAAAGGACACCCTCCAACTGGCGGCCAGGCGCCTGCAGCAAATACGCCATCAATTCCCCTATCGGTAGCGGTCTGCTGACCAGGAAGCCTTGCACCTGGTCGCAGCCAAACCCGCGcaac                                                                                >  2:17919/1‑148 (MQ=255)
                                          cgcgAAGAGGCCCTAAAGGACCCCCTCCAACTGGCGGCCCGGCGCCTGCAGCAAATACGCCATCAATTCCCCTATCGGTAGCGGTCTGCTGACCAGGCAGCCTTGCCCCTGGTCGCCGCCCAACCCGCGCAACAACGTCAGCTGCTCtt                                                                >  5:179334/1‑149 (MQ=255)
                                           gcgAAGAGGCCCTAAAGGACACCCTCCAACTGGCGGCCAGGCGCCTGCAGCAAATACGCCATCAATTCCCCTATCGGTAGCGGTCTGCTGACCAGGAAGCCTTGCACCTGGTCGCAGCCAAACCCGCGCAACAACGTCAGCTGCTCtt                                                                >  2:175525/1‑148 (MQ=255)
                                            cgAAGAGGCCCTAAAGGACACCCTCCAACTGGCGGCCAGGCGCCTGCAGCAAATACGCCATCAATTCCCCTATCGGTAGCGGTCTGCTGACCAGGAAGCCTTGCACCTGGTCGCAGCCAAACCCGCGCAACAACGTCAGCTGCTCTTCa                                                              <  8:25370/149‑1 (MQ=255)
                                            cgAAGAGGCCCTAAAGGACACCCTCCAACTGGCGGCCAGGCGCCTGCAGCAAATACGCCATCAATTCCCCTATCGGTAGCGGTCTGCTGACCAGGAAGCCTTGCACCTGGTCGCAGCCAAACCCGCGCAACAACGTCAGCTGCTCTTCa                                                              <  8:31090/149‑1 (MQ=255)
                                                   gCCCTAAAGGACACCCTCCACCTGGCGGCCAGGCGCCTGCAGCAAATACGCCATCAATTCCCCTCTCGGTAGCGGTCTGCTGACCAGGAAGCCTTGCACCTGGTCGCAGCCAAACCCGCGCAACAACGTCAGCTGCTCTTCACTCTCca                                                       <  6:179334/149‑1 (MQ=255)
                                                    cccTAAAGGACACCCTCCAACTGGCGGCCAGGCGCCTGCAGCAAATACGCCATCAATTCCCCTATCGGTAGCGGTCTGCTGACCAGGAAGCCTTGCACCTGGTCGCAGCCAAACCCGCGCAACAACGTCa                                                                         <  6:221545/130‑1 (MQ=255)
                                                    cccTAAAGGACACCCTCCAACTGGCGGCCAGGCGCCTGCAGCAAATACGCCATCAATTCCCCTATCGGTAGCGGTCTGCTGACCAGGAAGCCTTGCACCTGGTCGCAGCCAAACCCGCGCAACAACGTCa                                                                         >  5:221545/1‑130 (MQ=255)
                                                     ccTAAAGGACACCCTCCAACTGGCGGCCAGGCGCCTGCAGCAAATACGCCATCAATTCCCCTATCGGTAGCGGTCTGCTGACCAGGAAGCCTTGCACCTGGTCGCAGCCAAACCCGCGCaacaac                                                                             <  7:197969/125‑1 (MQ=255)
                                                     ccTAAAGGACACCCTCCAACTGGCGGCCAGGCGCCTGCAGCAAATACGCCATCAATTCCCCTATCGGTAGCGGTCTGCTGACCAGGAAGCCTTGCACCTGGTCGCAGCCAAACCCGCGCaacaac                                                                             >  8:197969/1‑125 (MQ=255)
                                                     ccTAAAGGACACCCTCCAACTGGCGGCCAGGCGCCTGCAGCAAATACGCCATCAATTCCCCTATCGGTAGCGGTCTGCTGACCAGGAAGCCTTGCACCTGGTCGCAGCCAAACCCGCGCAACAACGTCAgctgct                                                                   >  3:310670/1‑135 (MQ=255)
                                                     ccTAAAGGACACCCTCCAACTGGCGGCCAGGCGCCTGCAGCAAATACGCCATCAATTCCCCTATCGGTAGCGGTCTGCTGACCAGGAAGCCTTGCACCTGGTCGCAGCCAAACCCGCGCAACAACGTCAgctgct                                                                   <  4:310670/135‑1 (MQ=255)
                                                     ccTAAAGGACACCCTCCAACTGGCGGCCAGGCGCCTGCAGCAAATACGCCATCAATTCCCCTATCGGTAGCGGTCTGCTGACCAGGAAGCCTTGCACCTGGTCGCAGCCAAACCCGCGCAACAACGTCAGCTGCTCTTCa                                                              >  3:341010/1‑140 (MQ=255)
                                                     ccTAAAGGACACCCTCCAACTGGCGGCCAGGCGCCTGCAGCAAATACGCCATCAATTCCCCTATCGGTAGCGGTCTGCTGACCAGGAAGCCTTGCACCTGGTCGCAGCCAAACCCGCGCAACAACGTCAGCTGCTCTTCa                                                              <  4:341010/140‑1 (MQ=255)
                                                               acCCTCCAACTGGCGGCCAGGCGCCTGCAGCAAATACGCCATCAATTTCCCTATCGGTAGCGGTCTGCTGACCAGGAAGCCTTGCACCTGGTCGCAGCCAAACCCGCGCAACAACGTCAGCTGCTCTTCACTCTCCACACCCTCGGcca                                           >  3:327707/1‑149 (MQ=255)
                                                               acCCTCCAACTGGCGGCCAGGCGCCTGCAGCAAATACGCCATCAATTTCCCTATCGGTAGCGGTCTGCTGACCAGGAAGCCTTGCACCTGGTCGCAGCCAAACCCGCGCAACAACGTCAGCTGCTCTTCACTCTCCACACCCTCGGcca                                           >  3:322486/1‑149 (MQ=255)
                                                                   tCCAACTGGCGGCCAGGCGCCTGCAGCAAATACGCCATCAATTCCCCTATCGGTAGCGGTCTGCTGACCAGGAAGCCTTGCACCTGGTCGc                                                                                                 >  4:249248/1‑91 (MQ=255)
                                                                   tCCAACTGGCGGCCAGGCGCCTGCAGCAAATACGCCATCAATTCCCCTATCGGTAGCGGTCTGCTGACCAGGAAGCCTTGCACCTGGTCGc                                                                                                 <  3:249248/91‑1 (MQ=255)
                                                                   tCCAACTGGCGGCCAGGCGCCTGCAGCAAATACGCCATCAATTCCCCTATCGGTAGCGGTCTGCTGACCAGGAAGCCTTGCACCTGGTCGCAGCCAAACCCGCGCAACAACGTCAGCTGCTCTTCACtctc                                                         >  7:243113/1‑131 (MQ=255)
                                                                   tCCAACTGGCGGCCAGGCGCCTGCAGCAAATACGCCATCAATTCCCCTATCGGTAGCGGTCTGCTGACCAGGAAGCCTTGCACCTGGTCGCAGCCAAACCCGCGCAACAACGTCAGCTGCTCTTCACtctc                                                         <  8:243113/131‑1 (MQ=255)
                                                                   tCCAACTGGCGGCCAGGCGCCTGCAGCAAATACGCCATCAATTCCCCTATCGGTAGCGGTCTGCTGACCAGGAAGCCTTGCACCTGGTCGCAGCCAAACCCGCGCAACAACGTCAGCTGCTCTTCACTCTCCACACCCTCGGCcaccac                                       >  1:173852/1‑149 (MQ=255)
                                                                      aaCTGGCGGCCAGGCGCCTGCAGCAAATACGCCATCAATTCCCCTATCGGTAGCGGTCTGCTGACCAGGAAGCCTTGCACCTGGTCGCAGCCAAACCCGCGCAACAACGTCAGCTGCTCTTCACTCTCca                                                       <  4:161661/130‑1 (MQ=255)
                                                                      aaCTGGCGGCCAGGCGCCTGCAGCAAATACGCCATCAATTCCCCTATCGGTAGCGGTCTGCTGACCAGGAAGCCTTGCACCTGGTCGCAGCCAAACCCGCGCAACAACGTCAGCTGCTCTTCACTCTCCACACCCTCGGCCACCACCTc                                    <  5:66264/149‑1 (MQ=255)
                                                                      aaCTGGCGGCCAGGCGCCTGCAGCAAATACGCCATCAATTCCCCTATCGGTAGCGGTCTGCTGACCAGGAAGCCTTGCACCTGGTCGCAGCCAAACCCGCGCAACAACGTCAGCTGCTCTTAACTCTCCACACCCACGGCCaccaccac                                    >  6:66264/1‑147 (MQ=255)
                                                                      aaCTGGCGGCCAGGCGCCTGCAGCAAATACGCCATCAAATCCCCTATCGGTAGCGGTCTGCTGACCAGGAAGCCTTGCACCTGGTCGCAGCCAAACCCGCGCAACAACGTCAGCTGCTCTTCACTCTCca                                                       >  3:161661/1‑130 (MQ=255)
                                                                         tGGCGGCCAGGCGCCTGCAGCAAATACGCCATCAATTCCCCTATCGGTAGCGGTCTGCTGACCAGGAAGCCTTGCACCTGGTCGCAGCCAAACCCGCGCAACAACGTCAGCTGCTCTTCACTCTCCACACCCTCGGCCACCACCTCgag                                 >  1:53852/1‑149 (MQ=255)
                                                                           gcggcCAGGCGCCTGCAGCAAATACGCCATCAATTCCCCTATCGGTAGCGGTCTGCTGACCAGGAAGCCTTGCACCTGGTCGCAGCCAAACCCGCGCAACAACGTCAGCTGCTCTTCACTCTCCACACCCTCGGCCACCACCTCgaggt                               >  6:188954/1‑149 (MQ=255)
                                                                                   gcgcCTGCAGCAAATACGCCATCAATTCCCCTATCGGTAGCGGTCTGCTGACCAGGAAGCCTTGCACCTGGTCGCAGCCAAACCCGCGCAACAACGTCAGCTGCTCTTCACTCTCCACACCCTCGGCCACCACCTCGaggttgaggttg                       <  2:19057/149‑1 (MQ=255)
                                                                                    cgcCTGCAGCAAATACGCCATCAATTCCCCTATCGGTAGCGGTCTGCTGACCAGGAAGCCTTGCACCTGGTCGCAGCCAAACCCGCGCAACAACGTCAGCTGCTCTTCACTCTCCACACCCTCGGCCACCACCTCGAGGTTGAGGTtgt                      <  1:52882/149‑1 (MQ=255)
                                                                                      ccTGCAGCAAATACGCCATCAATTTCCCTATCGGTAGCGGTCTGCTGACCAGGAAGCCTTGCACCTGGTCGCAGCCAAACCCGCGCAACAACGTCAGCTGCTCTTCACTCTCCACACCCTCGGCCACCACCTCGAGGTTGAGGTTGTgc                    <  4:327707/149‑1 (MQ=255)
                                                                                      ccTGCAGCAAATACGCCATCAATTTCCCTATCGGTAGCGGTCTGCTGACCAGGAAGCCTTGCACCTGGTCGCAGCCAAACCCGCGCAACAACGTCAGCTGCTCTTCACTCTCCACACCCTCGGCCACCACCTCGAGGTTGAGGTTGTgc                    <  4:322486/149‑1 (MQ=255)
                                                                                      ccTGCAGCAAATACGCCATCAATTCCCCTATCGGTAGCGGTCTGCTGACCAGGAAGCCTTGCACCTGGTCGCAGCCAAACCCGCGCAACAACGTCAGCTGCTCTTCACTCTCCACACCCTCGGCCACCACCTCGAGGTTGAGGTTGTgc                    <  3:154433/149‑1 (MQ=255)
                                                                                         gcagcaAATACGCCATCAATTCCCCTATCGGTAGCGGTCTGCTGACCAGGAAGCCTTGCACCTGGTCGCAGCCAAACCCGCGCAACAACGTCAGCTGCTCTTCACTCTCCACACCCTCGGCCACCACCTCGAGGTTGAGGTTGTgcgc                  >  1:136961/1‑148 (MQ=255)
                                                                                            gcaAATACGCCATCAATTCCCCTATCGGTAGCGGTCTGCTGACCAGGAAGCCTTGCACCTGGTCGCAGCCAAACCCGCGCAACAACGTCAGCTGCTCTTCACTCTCCACACCCTCGGCCACCACCTCGAGGTTGAGGTTGTGCGCCAgg              >  3:234057/1‑149 (MQ=255)
                                                                                             caAATACGCCATCAATTCCCCTATCGGTAGCGGTCTGCTGACCAGGAAGCCTTGCACCTGGTCGCAGCCAAACCCGCGCAACAACGTCAGCTGCTCTTCACTCTCCACACCCTCGGCCACCACCTCGAGGTTGAGGTTGTGCGCCAGGt             <  5:188954/149‑1 (MQ=255)
                                                                                             caAATACGCCATCAATTCCCCTATCGGTAGCGGTCTGCTGACCAGGAAGCCTTGCACCTGGTCGCAGCCAAACCCGCGCAACAACGTCAGCTGCTCTTCACTCTCCACACCCTCGGCCACCACCTCGAGGTTGAGGTTGTGCGCCAGGt             <  1:175525/149‑1 (MQ=255)
                                                                                                    gCCATCAATTCCCCTATCGGTAGCGGTCTGCTGACCAGGAAGCCTTGCACCTGGTCGCAGCCAAACCCGCGCAACAACGACAGCTGCTCTTCACTCTCCACACCCTCGGCCACCACCTCGAGGTTGAGGTTGTGCGCCAGGTTGATCAt      >  8:9332/1‑149 (MQ=255)
                                                                                                      cATCAATTCCCCTATCGGTAGCGGTCTGCTGACCAGGAAGCCTTGCACCTGGTCGCAACCAAACCCGCGCAACAACGTCAGCTGCTCTTCACTCTCCACACCCTCGGCCACCACCTCGAGGTTGAGGTTGTGCGCCAGGTTGATCATgg    >  8:206724/1‑149 (MQ=255)
                                                                                                      cATCAATTCCCCTAACGGTAGCGGTCTGCTGACCAGGAAGCCTTGCACCTGGTCGCAGCCAAACCCGCGCAACAACGTCAGCTGCTCTTCACTCTCCACACCCTCGGCCACCTCCTCGAGGTTGAGGTTGTGCGCCAGGTTGATCATgg    >  5:170215/1‑149 (MQ=255)
                                                                                                       aTCAATTCCCCTATCGGTAGCGGTCTGCTGACCAGGAAGCCTTGCACCTGGTCGCAGCCAAACCCGCGCAACAACGTCAGCTGCTCTTCACTCTCCACACCCTCGGCCACCACCTCGAGGTTGAGGTTGTGCGCCAGGTTGATCATGGc   <  1:17919/149‑1 (MQ=255)
                                                                                                        tCAATTCCCCTATCGGTAGCGGTCTGCTGACCAGGAAGCCTTGCACCTGGTCGCAGCCAAACCCGCGCAACAACGTCAGCTGCTCTTCACTCTCCACACCCTCGGCCACCACCTCGAGGTTGAGGTTGTGCGCCAGGTTGATCATGGCg  >  7:205436/1‑149 (MQ=255)
                                                                                                           aTTCCCCTATCGGTAGCGGTCTGCTGACCAGGAAGCCTTGCACCTGGTCTCAGCCAAACCCGCGCAACAACGTCAGCTGCTCTTCACTCTCCACACCCTCg                                               <  4:174869/101‑1 (MQ=255)
                                                                                                           aTTCCCCTATCGGTAGCGGTCTGCTGACCAGGAAGCCTTGCACCTGGTCGCAGCCAAACCCGCGCAACAACGTCAGCTGCTCTTCACTCTCCACACCCTCg                                               >  3:174869/1‑101 (MQ=255)
                                                                                                              |                                                                                                                                              
GCTCCCACAGGATTGCGCGGTCCCTGTGGGAGCGGGCGTGCCCGCGAAGAGGCCCTAAAGGACACCCTCCAACTGGCGGCCAGGCGCCTGCAGCAAATACGCCATCAATTCCCCTATCGGTAGCGGTCTGCTGACCAGGAAGCCTTGCACCTGGTCGCAGCCAAACCCGCGCAACAACGTCAGCTGCTCTTCACTCTCCACACCCTCGGCCACCACCTCGAGGTTGAGGTTGTGCGCCAGGTTGATCATGGCG  >  NC_002947/6009131‑6009383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑