Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,011,493 G→C 10.9% K115N (AAG→AAC)  rep → single‑stranded DNA dependent ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,011,4930GC10.9% 150.3 / 9.3 55K115N (AAG→AAC) repsingle‑stranded DNA dependent ATPase
Reads supporting (aligned to +/- strand):  ref base G (25/24);  new base C (2/4);  total (27/28)
Fisher's exact test for biased strand distribution p-value = 6.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.76e-01

GGTCGCCACCTTGCTGCGCCCGGGGGAAGGCCGTGGCCTGACCGTGTGTACGTTCCACAACCTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTGGAAGAACGA‑CCTGGTCCTGCCGCCAGAAGCCCTGGAAAAGGCGCGCAACCCGCGCGAGCAGAC  >  NC_002947/6011349‑6011636
                                                                                                                                                |                                                                                                                                                
ggTCGCCACCTTGCTGCGCCCGGGGGAAGGCCGTGGCCTGACCGTGTGTACGTTCCACAACCTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGt                                                                                                                                              <  5:62506/149‑1 (MQ=255)
  tCGCCACCTTGCTGCGCCCGGGGGAAGGCCGTGGCCTGACCGTGTGTACGTTCCACAACCTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTg                                                                                                                                            <  4:279796/149‑1 (MQ=255)
     ccACCTTGCTGCGCCCGGGGGAAGGCCGTGGCCTGACCGTGTGTACGTTCCACAACCTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTg                                                                                                                                         >  7:170336/1‑149 (MQ=255)
       aCCTTGCTGCGCCCGGGGGAAGGCCGTGGCCTGACCGTGTGTACGTTCCACAACCTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTc                                                                                                                                       <  3:347147/149‑1 (MQ=255)
            gctgcGCCCGGGGGAAGGCCGTGGCCTGACCGTGTGTACGTTCCACAACCTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTGGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGAca                                                                                                                                  >  5:320856/1‑149 (MQ=255)
            gctgcGCCCGGGGGAAGGCCGTGGCCTGACCGTGTGTACGTTCCACAACCTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACAGCAACGCCTTGATGTCGGa                                                                                                                                    <  3:195514/147‑1 (MQ=255)
            gctgcGCCCGGGGGAAGGCCGTGGCCTGACCGTGTGTACGTTCCACAACCTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACAGCAACGCCTTGATGTCGGAc                                                                                                                                   <  1:123016/148‑1 (MQ=255)
              tgcGCCCGGGGGAAGGCCGTGGCCTGACCGTGTGTACGTTCCACAACCTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGAcat                                                                                                                                 >  3:257896/1‑148 (MQ=255)
                  cccGGGGGAAGGCCGTGGCCTGACCGTGTGTACGTTCCACAACCTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATg                                                                                                                             >  5:291844/1‑148 (MQ=255)
                         gAAGGCCGTGGCCTGACCGTGTGTACGTTCCACAACCTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAgg                                                                                                                      >  1:192453/1‑148 (MQ=255)
                          aaGGCCGTGGCCTGACCGTGTGTACGTTCCACAACCTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGaa                                                                                                                    <  6:6517/149‑1 (MQ=255)
                                   gCCTGACCGTGTGTACGTTCCACAACCTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCgg                                                                                                            <  2:192453/148‑1 (MQ=255)
                                   gCCTGACCGTGTGTACGTTCCACAACCTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGc                                                                                                           <  3:345753/149‑1 (MQ=255)
                                          cGTGTGTACGTTCCACAACCTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGacgacg                                                                                                    <  8:170336/149‑1 (MQ=255)
                                             gtgtACGTTCCACAACCTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATg                                                                                                                             <  4:290108/121‑1 (MQ=255)
                                             gtgtACGTTCCACAACCTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATg                                                                                                                             >  3:290108/1‑121 (MQ=255)
                                             gtgtACGTTCCACAACCTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACAGCAACGCCTTg                                                                                                                                            >  1:124920/1‑106 (MQ=255)
                                             gtgtACGTTCCACAACCTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACAGCAACGCCTTg                                                                                                                                            <  2:124920/106‑1 (MQ=255)
                                                   ggtCCACAACCTGGGCCTGAACATCATCCGCAAGCAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAgg                                                                                                                      <  5:230411/120‑1 (MQ=255)
                                                   gTTCCACAACCTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAgg                                                                                                                      >  6:230411/1‑122 (MQ=255)
                                                     tCCACAACCTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCAACGACGGCATcgac                                                                                            >  7:273129/1‑146 (MQ=255)
                                                       cacaACCTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATcgac                                                                                            >  6:233067/1‑144 (MQ=255)
                                                        acaACCTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATATGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAg                                                                                                                       >  7:175118/1‑116 (MQ=255)
                                                        acaACCTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATATGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAg                                                                                                                       <  8:175118/116‑1 (MQ=255)
                                                          aaCCTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACgaga                                                                                        <  2:121720/145‑1 (MQ=255)
                                                          aaCCTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACgaga                                                                                        >  1:121720/1‑145 (MQ=255)
                                                                   cTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGAt                                                                           >  8:288622/1‑149 (MQ=255)
                                                                      aaCATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGATGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGaa                                                                                                                    <  3:270844/105‑1 (MQ=255)
                                                                      aaCATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGATGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGaa                                                                                                                    >  4:270844/1‑105 (MQ=255)
                                                                                 cAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTGgaag                                                              >  3:323748/1‑148 (MQ=255)
                                                                                 cAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTGgaag                                                              >  3:366496/1‑148 (MQ=255)
                                                                                      aaCACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTGGAAGAACGA‑c                                                       <  6:320856/149‑1 (MQ=255)
                                                                                       acacGCGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAGTACTCCGGCGACGACGGCATCGACGAGATGCAGGACAGGATCGGTGCCTGgaaggccgccc                                                       >  6:380362/1‑142 (MQ=255)
                                                                                         acGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCTACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTGGAAGAACGA‑CCTg                                                    <  8:273129/149‑1 (MQ=255)
                                                                                            aGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTGGAAGAACGA‑CCTGGTc                                                 <  7:288622/149‑1 (MQ=255)
                                                                                             gcgcCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTGGAAGAACGA‑CCTGGTcc                                                >  7:6823/1‑149 (MQ=255)
                                                                                                              cGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTGGAAGAACGA‑CCTGGTCCTGCCGCCAGAAGCCCTg                               <  4:366496/149‑1 (MQ=255)
                                                                                                               gggTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTGGAAGAACGA‑CCTGGTCCTGCCGCCAg                                       <  4:31349/140‑1 (MQ=255)
                                                                                                               gggTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTGGAAGAACGA‑CCTGGTCCTGCCGCCAg                                       >  3:31349/1‑140 (MQ=255)
                                                                                                                    tctcGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTGGAAGAACGA‑CCTGGTCCTGCCGCCAGAAGCCCTGGaaaa                          <  4:257896/148‑1 (MQ=255)
                                                                                                                     ctcGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTGGAAGAACGA‑CCTGGTCCTGCCGCCAGAAGCCCTGGAAAAgg                        >  7:278279/1‑149 (MQ=255)
                                                                                                                      tcGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTGGAAGAACGA‑CCTGGTCCTGCCGCCAGAAGCCCTGGAAAAGgc                       >  1:53728/1‑149 (MQ=255)
                                                                                                                      tcGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTGGAAGAACGA‑CCTGGTCCTGCCGCCAGAAGCCCTGGAAAAGgc                       >  2:240978/1‑149 (MQ=255)
                                                                                                                        gATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGc                                                                     <  6:279938/102‑1 (MQ=255)
                                                                                                                        gATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGc                                                                     >  5:279938/1‑102 (MQ=255)
                                                                                                                        gATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTGGAAGAACGA‑CCTGGTCCTGCCGCCAGAAGCCCTGGAAAAGgc                       <  8:6823/147‑1 (MQ=255)
                                                                                                                              cgacgaGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTGGAAGAACGA‑CCTGGTCCTGCCGCCAGAAGCCCTGGAAAAGgcgc                     <  4:323748/143‑1 (MQ=255)
                                                                                                                                    gTCCGACAGCAACGCCTTGATGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTGGAAGAACGA‑CCTGGTCCTGCCGCCAGAAGCCCTGGAAAAGGCGCGCAACCcgcgcg         >  2:283368/1‑149 (MQ=255)
                                                                                                                                     tCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATc                                                                          <  6:143412/84‑1 (MQ=255)
                                                                                                                                     tCCGACATCAAGGCGTAGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATc                                                                          >  5:143412/1‑84 (MQ=255)
                                                                                                                                       cGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTGGAAGAACGA‑CCTGGTCCTGCCGCCAGAAGCCCTGGAAAAGGCGCGCAACCCGCGCGAg       <  8:278279/148‑1 (MQ=255)
                                                                                                                                        gACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATcgac                                                                                            >  7:151922/1‑63 (MQ=255)
                                                                                                                                        gACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATcgac                                                                                            <  8:151922/63‑1 (MQ=255)
                                                                                                                                           aTCAAGGCGTTGCTGTCGGACATCATGGAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTGGAAGAACGA‑CCTGGTCCTGCCGCCAGAAGCCCTGGAAAAGGCGCGCAACCCGCGCGAGCAGAc  >  8:224005/1‑149 (MQ=255)
                                                                                                                                            gcAACGCCTTGATGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTGGAAGAACGACCCTGGTCCTGCCGCCAGAAGCCCTGGAAAAGGCGCGCAACCCGCGCGAGCAGAc  <  1:283368/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
GGTCGCCACCTTGCTGCGCCCGGGGGAAGGCCGTGGCCTGACCGTGTGTACGTTCCACAACCTGGGCCTGAACATCATCCGCAAGGAACACGAGCGCCTGGGCTATAAGCCGGGTTTCTCGATCTTCGACGAGTCCGACATCAAGGCGTTGCTGTCGGACATCATGCAGAAGGAATACTCCGGCGACGACGGCATCGACGAGATCAAGAACATGATCGGTGCCTGGAAGAACGA‑CCTGGTCCTGCCGCCAGAAGCCCTGGAAAAGGCGCGCAACCCGCGCGAGCAGAC  >  NC_002947/6011349‑6011636

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑