BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	507,245	C→T	6.3%	R589Q (CGG→CAG)	estP ←	esterase EstP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	507,245	0	C	T	6.3%	288.8 / 6.2	95	R589Q (CGG→CAG)	estP	esterase EstP
Reads supporting (aligned to +/- strand): ref base C (44/45); new base T (3/3); total (47/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.64e-01

GGCCGCAAAGCGGCCCCCAGCAATTCAGAAGTCCACGCTAACCCCCACGCTAATACCCTGTTGCGTCAACTCATCACTCTTGCGCCAGTTGTAGTTCCCGCGCAAATGCACCCCTGCCACCAGTTCATGGCTCACACCCAGGCTCGCCCGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTGAAGTCATTCGCCGGCAAGCTGGTCAGGTGCATCGTCACATCCTGCTCGTCGTCCTCGAACTCATGCTCCTGCGCCACCTCGGCGAAAAGCCGGGTACTTGGCAGTACCTG  >  NC_002947/507097‑507393

ggCCGCAAAGCGGCCCCCAGCAATTCAGAAGTCCACGCTAACCCCCACGCTAATACCCTGTTGCGTCAACTCATCACTCTTGCGCCAGTTGTAGTTCCCGCGCAAATGCACCCCTGCCACCAGTTCATGGCTCACACCCAGGCTCGccc                                                                                                                                                      <  6:94067/149‑1 (MQ=255)

 gCCGCAAAGCGGCCCCCAGCAATTCAGAAGTCCACGCTAACCCCCACGCTAATACCCTGTTGCGTCAACTCATCACTCTTGCGCCAGTTGTAGTTCCCGCGCAAATGCACCCCTGCCACCAGTTCATGGCTCACACCCAGGCTCGCCCg                                                                                                                                                     <  4:295757/149‑1 (MQ=255)

  ccGCAAAGCGGCCCCCAGCAATTCAGAAGTCCACGCTAACCCCCACGCTAATACCCTGTTGCGTCAACTCATCACTCTTGCGCCAGTTGTAGTTCCCGCGCAAATGCACCCCTGCCACCAGTTCATGGCTCACACCCAGGCTCGCCCgg                                                                                                                                                    <  4:349320/149‑1 (MQ=255)

  ccGCAAAGCGGCCCCCAGCAATTCAGAAGTCCACGCTAACCCCCACGCTAATACCCTGTTGCGTCAACTCATCACTCTTGCGCCAGTTGTAGTTCCCGCGCAAATGCACCCCTGCCACCAGTTCATGGCTCACACCCAGGCTCGCCCgg                                                                                                                                                    <  4:343928/149‑1 (MQ=255)

  ccGCAAAGCGGCCCCCAGCAATTCAGAAGTCCACGCTAACCCCCACGCTAATACCCTGTTGCGTCAACTCATCACTCTTGCGCCAGTTGTAGTTCCCGCGCAAATGCACCCCTGCCACCAGTTCATGGCTCACACCCAGGCTCGCCCgg                                                                                                                                                    <  8:12808/149‑1 (MQ=255)

   cGCAAAGCGGCCCCCAGCAATTCAGAAGTCCACGCTAACCCCCACGCTAATACCCTGTTGCGTCAACTCATCACTCTTGCGCCAGTTGTAGTTCCCGCGCAAATGCACCCCTGCCACCAGTTCATGGCTCACACCCAGGCTCGCCCggg                                                                                                                                                   <  5:162187/149‑1 (MQ=255)

    gCAAAGCGGCCCCCAGCAATTCAGAAGTCCACGCTAACCCCCACGCTAATACCCTGTTGCGTCAACTCATCACTCTTGCGCCAGTTGTAGTTCCCGCGCAAATGCACCCCTGCCACCAGTTCATGGCTCACACCCAGGCTCGCCCGGGt                                                                                                                                                  <  5:300956/149‑1 (MQ=255)

     cAAAGCGGCCCCCAGCAATTCAGAAGTCCACGCTAACCCCCACGCTAATACCCTGTTGCGTCAACTCATCACTCTTGCGCCAGTTGTAGTTCCCGCGCAAATGCACCCCTGCCACCAGTTCATGGCTCACACCCGGGCGAGCCTGGGtg                                                                                                                                                 <  3:258962/149‑2 (MQ=255)

      aaaGCGGCCCCCAGCAATTCAGAAGTCCACGCTAACCCCCACGCTAATACCCTGTTGCGTCAACTCATCACTCTTGCGCCAGTTGTAGTTCCCGCGCAAATGCACCCCTGCCACCAGTTCATGGCTCACACCCAGGCTCGCCCGGGTCa                                                                                                                                                <  1:144105/149‑1 (MQ=255)

        aGCGGCCCCCAGCAATTCAGAAGTCCACGCTAACCCCCACGCTAATACCCTGTTGCGTCAACTCATCACTCTTGCGCCAGTTGTAGTTCCCGCGCAAATGCACCCCTGCCACCAGTTCATGGCTCACACCCAGGCGAGCCTGGGtgtga                                                                                                                                              <  8:188757/149‑5 (MQ=255)

              ccccAGCAATTCAGAAGTCCACGCTAACCCCCACGCTAATACCCTGTTGCGTCAACTCATCACTCTTGCGCCAGTTGTAGTTCCCGCGCAAATGCACCCCTGCCACCAGTTCATGGCTCACACCCAGGCTCGCCCGGGTCAGGt                                                                                                                                             <  1:126105/144‑1 (MQ=255)

              ccccAGCAATTCAGAAGTCCACGCTAACCCCCACGCTAATACCCTGTTGCGTCAACTCATCACTCTTGCGCCAGTTGTAGTTCCCGCGCAAATGCACCCCTGCCACCAGTTCATGGCTCACACCCAGGCTCGCCCGGGTCAGGt                                                                                                                                             >  2:126105/1‑144 (MQ=255)

                      aTTCAGAAGTCCACGCTAACCCCCACGCTAATACCCTGTTGCGTCAACTCATCACTCTTGCGCCAGTTGTAGTTCCCGCGCAAATGCACCCCTGCCACCAGTTCATGGCTCACACCCAGGCTCGCCCGGGTCAGGTCGCTGTgcggcg                                                                                                                                 >  7:250547/1‑148 (MQ=255)

                         cAGAAGTCCACGCTAACCCCCACGCTAATACCCTGTTGCGTCAACTCATCACTCTTGCGCCAGTTGTAGTTCCCGCGCAAATGCTCCCCTGCCACCAGTTCATGGCTCACACCCAGGCTCGCCCGGGTCAGGTCGCTGTGCGGCGtat                                                                                                                              <  1:124967/148‑1 (MQ=255)

                          aGAAGTCCACGCTAACCCCCACGCTAATACCCTGTTGCGTCAACTCATCACTCTTGCGCCAGTTGTAGTTCCCGCGCAAATGCACCCCTGCCACCAGTTCATGGCTCACACCCAGGCTCGCCCGGGTCAGGTCGCTGTGCGGCGTATAc                                                                                                                            <  7:87542/149‑1 (MQ=255)

                          aGAAGTCCACGCTAACCCCCACGCTAATACCCTGTTGCGTCAACTCATCACTCTTGCGCCAGTTGTAGTTCCCGCGCAAATGCACCCCTGCCACCAGTTCATGGCTCACACCCAGGCTCGACCGGCTCAGGTCGCAGTgcggctaaaa                                                                                                                             >  2:66047/1‑143 (MQ=255)

                              gTCCACGCTAACCCCCACGCTAATACCCTGTTGCGTCAACTCATCACTCTTGCGCCAGTTGTAGTTCCCGCGCAAATGCACCCCTGCCACCAGTTCATGGCTCACACCCAGGCTCGCCCGGGTCAGGTCGCTGTGCGGCGTATACCCgg                                                                                                                        >  4:250004/1‑149 (MQ=255)

                               tCCACGCTAACCCCCACGCTAATACCCTGTTGCGTCAACTCATCACTCTTGCGCAAGTTGTAGTTCCCGCGCAAATGCACCCCTGCCACCAGTTCATGGCTCACACCCAGGCTCGCCCGGGTCAGGTCGCTGTGCGGCGTATACCCgg                                                                                                                        >  6:139533/1‑148 (MQ=255)

                                ccACGCTAACCCCCACGCTAATACCCTGTTGCGTCAACTCATCACTCTTGCGCCAGTTGTAGTTCCCGCGCAAATGCACCCCTGCCACCAGTTCATGGCTCACACCCAGGCTCGCCCGGGTCAGGTCGCTGTGCGGCGTATACCCGGTc                                                                                                                      <  8:250547/149‑1 (MQ=255)

                                   cGCTAACCCCCACGCTAATACCCTGTTGCGTCAACTCATCACTCTTGCGCCAGTTGTAGTTCCCGCGCAAATGCACCCCTGCCACCAGTTCATGGCTCACACCCAGGCTCGCCCGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAgg                                                                                                                   >  6:36694/1‑149 (MQ=255)

                                     cTAACCCCCACGCTAATACCCTGTTGCGTCAACTCATCACTCTTGCGCCAGTTGTAGTTCCCGCGCAAATGCACCCCTGCCACCAGTTCATGGCTCACACCCAGGCTCGCCCGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGt                                                                                                                 <  5:36694/149‑1 (MQ=255)

                                        aCCCCCACGCTAATACCCTGTTGCGTCAACTCATCACTCTTGCGCCAGTTGTAGTTCCCGCGCAAATGCACCCCTGCCACCAGTTCATGGCTCACACCCAGGCTCGCCCGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTg                                                                                                                >  1:175153/1‑147 (MQ=255)

                                        aCCCCCACGCTAATACCCTGTTGCGTCAACTCATCACTCTTGCGCCAGTTGTAGTTCCCGCGCAAATGCACCCCTGCCACCAGTTCATGGCTCACACCCAGGCTCGCCCGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTGaa                                                                                                              <  2:87252/149‑1 (MQ=255)

                                               cGCTAATACCCTGTTGCGTCAACTCATCACTCTTGCGCCAGTTGTAGTTCCCGCGCAAATGCACCCCTGCCACCAGTTCATGGCTCACACCCAGGCTCGCCCGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTGAAGTCATTc                                                                                                       <  5:48099/149‑1 (MQ=255)

                                                    aTACCCTGTTGCGTCAACTCATCACTCTTGCGCCAGTTGTAGTTCCCGCGCAAATGCACCCCTGCCACCAGTTCATGGCTCACACCCAGGCTCGCCCGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTGAAGTCATTCGCCgg                                                                                                  <  5:50632/149‑1 (MQ=255)

                                                    aTACCCTGTTGCGTCAACTCATCACTCTTGCGCCAGTTGTAGTTCCCGCGCAAATGCACCCCTGCCACCAGTTCATGGCTCACACCCAGGCTCGCCCGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTGAAGTCATTCGCCg                                                                                                   <  2:175153/148‑1 (MQ=255)

                                                              gCGTCAACTCATCACTCTTGCGCCAGTTGTAGTTCCCGCGCAAATGCACCCCTGCCACCAGTTCATGGCTCACACCCAGGCTCGCCCGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTGAAGTCATTCGCCGGCAAGCTGGt                                                                                         <  1:66047/148‑1 (MQ=255)

                                                               cGTCAACTCATCACTCTTGCGCCAGTTGTAGTTCCCGCGCAAATGCACCCCTGCCACCAGTTCATGGCTCACACCCAGGCTCGCCCGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTGAAGTCATTCGCCGGCAAGCTGGt                                                                                         >  7:275221/1‑147 (MQ=255)

                                                                gTCAACTCATCACTCTTGCGCGAGTTGTAGTTCCCGAGCAAATGCACCCCTGCCACCAGTTCATGGCTCACACCCAGGCTCGCCCGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTGAAGTCATTCTCCGGCAAGCTGGTCa                                                                                       <  1:285884/148‑1 (MQ=255)

                                                                gTCAACTCATCACTCTTGCGCCAGTTGTATTTCCCGCGCAAATGCACCCCTGCCACCAGTTCATGGCTCACACACAAGCACCCACGGGTCAGCTCGCTGTGCGCCGAATACCCGGTCAGGGTGAAGTCATTCGCCGGCAAGCTGGt                                                                                         >  2:285884/1‑146 (MQ=255)

                                                                gTCAACTCATCACTCTTGCGCCAGTTGTAGTTCCCGCGCAAATGCACCCCTGCCACCAGTTCATGGCTCACACCCAGGCTCGCCCGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTGAAGTCATTCGCCGGCAAGCTGGTCAg                                                                                      >  6:361329/1‑149 (MQ=255)

                                                                     cTCATCACTCTTGCGCCAGTTGTAGTTCCCGCGCAAATGCACCCCTGCCACCAGTTCATGGCTCACACCCAGGCTCGCCCGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTGAAGTCATTCGCCGGCAAGCTGGTCAGGTGCa                                                                                 >  5:137482/1‑149 (MQ=255)

                                                                      tcatcaCTCTTGCGCCAGTTGTAGTTCCCGCGCAAATGCACCCCTGCCACCAGTTCATGGCTCACACCCAGGCTCGCCCGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTGAAGTCATTc                                                                                                       <  3:369295/126‑1 (MQ=255)

                                                                      tcatcaCTCTTGCGCCAGTTGTAGTTCCCGCGCAAATGCACCCCTGCCACCAGTTCATGGCTCACACCCAGGCTCGCCCGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTGAAGTCATTc                                                                                                       >  4:369295/1‑126 (MQ=255)

                                                                      tcatcaCTCTTGCGCCAGTTGTAGTTCCCGCGCAAATGCACCCCTGCCACCAGTTCATGGCTCACACCCAGGCTCGCCCGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTGAAGTCATTCGCCGGCAAGCTGGTCAGGTGCAt                                                                                >  4:181618/1‑149 (MQ=255)

                                                                      tcatcaCTCTTGCGCCAGTTGTAGTTCCCGCGCAAATGCACCCCTGCCACCAGTTCATGGCTCACACCCAGGCTCGCCCGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTGAAGTCATTCGCCGGCAAGCTGGTCAGGTGCAt                                                                                >  1:78057/1‑149 (MQ=255)

                                                                        atcaCTCTTGCGCCAGTTGTAGTTCCCGCGCAAATGCACCCCTGCCACCAGTTCATGGCTCACACCCAGGCTCGCCCGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTGAAGTCATTCGCCGGCAAGCTGGTCAGGTGCATCg                                                                              >  1:223431/1‑149 (MQ=255)

                                                                             tctTGCGCCAGTTGTAGTTCCCGCGCAAATGCACCCCTGCCACCAGTTCATGGCTCACACCCAGGCTCGCCCGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTGAAGTCATTCGCCGGCAAGCTGGTCAGGTGCATCGTcac                                                                          >  8:271964/1‑148 (MQ=255)

                                                                               ttGCGCCAGTTGTAGTTCCCGCGCAAATGCACCCCTGCCACCAGTTCATGGCTCACACCCAGGCTCGCCCGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTGAAGTCATTCGCCGGCAAGCTGGTCAGGTGCATCGTcaca                                                                         <  8:275221/147‑1 (MQ=255)

                                                                                   gcCAGTTGTAGTTCCCGCGCAAATGCACCCCTGCCACCAGTTCATGGCTCACACCCAGGCTCGCCCGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTGAAGTCATTCGCCGGCAAGCTGGTCAGGTGCATCGTCACATCCTGc                                                                   <  7:271964/149‑1 (MQ=255)

                                                                                   gcCAGTTGTAGTTCCCGCGCAAATGCACCCCTGCCACCAGTTCATGGCTCACACCCAGGCTCGCCCGGGTCAGGTCGCTGTGCGGCGTAAACCCGGTCAGGGTGAAGTCATTCGCCGGCAAGCTGGTCAGGTGCATCGTCACATCCTGc                                                                   <  1:11426/149‑1 (MQ=255)

                                                                                           tAGTTCCCGCGCAAATGCACCCCTGCCACCAGTTCATGGCTCACACCCAGGCTCGCCCGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTGAAGTCATTCGCCGGCAAGCTGGTCAGGTGCATCGTCACATCCTGCTCgtcgtc                                                           >  6:244022/1‑149 (MQ=255)

                                                                                                cccGCGCAAATGCACCCCTGCCACCAGTTCATGGCTCACACCCAGGCTCGCCCGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTGAAGTCATTCGCCGGCAAGCTGGTCAGGTGCATCGTCACATCCTGCTCGTCGTCCTCg                                                       >  8:205728/1‑148 (MQ=255)

                                                                                                 ccGCGCAAATGCACCCCTGCCACCAGTTCATGGCTCACACCCAGGCTCGCCCGTGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTGAAGTCATTCGCCGGCAAGCTGGTCAGGt                                                                                    <  7:84978/118‑1 (MQ=255)

                                                                                                 ccGCGCAAATGCACCCCTGACACCAGTTCATGGCTCACACCCAGGATCGCCCGGGTCAGGTAGCTGTGCGTCGTATACCCGGTCAGGGTGAAGTCATTCGACGGCAAGCTCGTCAgct                                                                                    >  8:84978/1‑116 (MQ=255)

                                                                                                  cgcgCAAATGCACCCCTGCCACCAGTTCATGGCTCACACCCAGGCTCGCCCGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTGAAGTCATTCGCCGGCAAGCTGGTCAGGTGCATCGTCACATCCTGCTCGTCGTCCTCGAAc                                                    >  6:57621/1‑149 (MQ=255)

                                                                                                  cgcgCAAATGCACCCCTGCCACCAGTTCATGGCTCACACCCAGGCTCGCCCGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTGAAGTCATTCGCCGGCAAGCTGGTCAGGTGCATCGTCACATCCTGCTCGTCGTCCTCGAAc                                                    <  5:57621/149‑1 (MQ=255)

                                                                                                  cgcgCAAATGCACCCCTGCCACCAGTTCATGGCTCACACCCAGGCTCGCCCGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTGAAGTCATTCGCCGGCAAGCTGGTCAGGTGCATCGTCACATCCTGCTCGTCGTCCTCGAAc                                                    >  6:291771/1‑149 (MQ=255)

                                                                                                   gcgcAAATGCACCCCTGCCACCAGTTCATGGCTCACACCCAGGCTCGCCCGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTGAAGTCATTCGCCGGCAAGCTGGTCAGGTGCATCGTCACATCCTGCTCGTCGTCCTCGAAc                                                    >  2:181353/1‑148 (MQ=255)

                                                                                                      cAAATGCACCCCTGCCACCAGTTCATGGCTCACACCCAGGCTCGCCCGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTGAAGTCATTCGCCGGCAAGCTGGTCAGGTGCATCGTCACATCCTGCTCGTCGTCCTCGAACTCAt                                                >  1:274585/1‑149 (MQ=255)

                                                                                                       aaaTGCACCCCTGCCACCAGTTCATGGCTCACACCCAGGCTCGCCCGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTGAAGTCATTCGCCGGCAAGCTg                                                                                           >  7:95304/1‑105 (MQ=255)

                                                                                                       aaaTGCACCCCTGCCACCAGTTCATGGCTCACACCCAGGCTCGCCCGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTGAAGTCATTCGCCGGCAAGCTg                                                                                           <  8:95304/105‑1 (MQ=255)

                                                                                                       aaaTGCACCCCTGCCACCAGTTCATGGCTCACACCCAGGCTCGCCCGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTGAAGTCATTCGCCGGCAAGCTGGTCAGGTGCATCGTCACATCCTGCTCGTCGTCCTCGAACTCATg                                               <  7:205728/149‑1 (MQ=255)

                                                                                                         aTGCACCCCTGCCACCAGTTCATGGCTCACACCCAGGCTCGCCCGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTGAAGTCATTCGCCGGCAAGCTGGTCAGGTGCATc                                                                               <  2:262132/115‑1 (MQ=255)

                                                                                                         aTGCACCCCTGCCACCAGTTCATGGCTCACACCCAGGCTCGCCCGCGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTGAAGTCATTCGCCGGCAAGCTGGTCAGGTGCATc                                                                               >  1:262132/1‑115 (MQ=255)

                                                                                                             aCCCCTGCCACCAGTTCATGGCTCACACCCAGGCTCGCCCGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTGAAGTCa                                                                                                          <  2:345635/84‑1 (MQ=255)

                                                                                                             aCCCCTGCCACCAGTTCATGGCTCACACCCAGGCTCGCCCGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTGAAGTCa                                                                                                          >  1:345635/1‑84 (MQ=255)

                                                                                                               cccTGCCACCAGTTCATGGCTCACACCCAGGCTCGCCCGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTGAAGTCATTCGCCGGCAAGCTGGTCAGGTGCATCGTCACATCCTGCTCGTCGTCCTc                                                        <  7:91520/132‑1 (MQ=255)

                                                                                                               cccTGCCACCAGTTCATGGCTCACACCCAGGCTCGCCCGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTGAAGTCATTCGCCGGCAAGCTGGTCAGGTGCATCGTCACATCCTGCTCGTCGTCCTc                                                        >  8:91520/1‑132 (MQ=255)

                                                                                                               cccTGCCACCAGTTCATGGCTCACACCCAGGCTCGCCCGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTGAAGTCATTCGCCGGCAAGCTGGTCAGGTGCATCGTCACATCCTGCTCGTCGTCCTCGAACTCATGc                                              <  1:368481/142‑1 (MQ=255)

                                                                                                               cccTGCCACCAGTTCATGGCTCACACCCAGGCTCGCCCGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTGAAGTCATTCGCCGGCAAGCTGGTCAGGTGCATCGTCACATCCTGCTCGTCGTCCTCGAACTCATGc                                              >  2:368481/1‑142 (MQ=255)

                                                                                                               cccTGCCACCAGTTCATGGCTCACACCCAGGCTCGCCCGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTGAAGTCATTCGCCGGCAAGCTGGTCAGGTGCATCGTCACATCCTGCTCGTCGTCCTCGAACTCATGCTCCTgcg                                       >  2:186033/1‑149 (MQ=255)

                                                                                                                 cTGCCACCAGTTCATGGCTCACACCCAGGCTCGCCCGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTGAAGTCATTCGCCGGCAAGCTGGTCAGGTGCATCGTCACATc                                                                       <  2:34312/115‑1 (MQ=255)

                                                                                                                 cTGCCACCAGTTCATGGCTCACACCCAGGCTCGCCCGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTGAAGTCATTCGCCGGCAAGCTGGTCAGGTGCATCGTCACATc                                                                       >  1:34312/1‑115 (MQ=255)

                                                                                                                    ccaccaGTTCATGGCTCACACCCAGGCTCGCCCGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTGAAGTCATTCGCCGGCAAGCTGGTCAGGTGCATCGTCACATCCTGCTCGTCGTCCTCGAACTCATGCTCCTGCGCCAcc                                  >  4:363577/1‑149 (MQ=255)

                                                                                                                      accaGTTCATGGCTCACACCCAGGCTCGCCCGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTGAAGTCATTCGCCGGCAAGCTGGTCAGGTGCATCGTCACATCCTGCTCGTCGTCCTCGAACTCATGCTCCTGCGCCACCTc                                >  8:229531/1‑149 (MQ=255)

                                                                                                                       ccaGTTCATGGCTCACACCCAGGCTCGCCCGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTGAAGTCATTCGCCGGCAAGCTGGTCAGGTGCATCGTCACATCCTGCTCGTCGTCCTCGAACTCATGCTCCTGCGCCACCTCg                               <  5:361329/149‑1 (MQ=255)

                                                                                                                        caGTTCATGGCTCACACCCAGGCTCGCCCGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTGAAGTCATTCGCCGGCAAGCTGGTCAGGTGCATCGTCACATCCTGCTCGTCGTCCTCGAACTCATGCTCCTGCGCCACCTCgg                              <  2:223431/149‑1 (MQ=255)

                                                                                                                          gTTCATGGCTCACACCCAGGCTCGCCCGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTGAAGTCATTCGCCGGCAAGCTGGTCAGGTGCATCGTCACATCCTGCTCGTCGTCCTCGAACTCATGCTCCTGCGCCACCTCGGCg                            <  5:291771/149‑1 (MQ=255)

                                                                                                                           ttCATGGCTCACACCCAGGCTCGCCCGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTGAAGTCATTCGCCGGCAAGCTGGTCAGGTGCATCGTCACATCCTGCTCGTCGTCCTCGAACTCATGCTCCTGCGCCACCTCGGCg                            >  6:186367/1‑148 (MQ=255)

                                                                                                                              aTGGCTCACACCCAGGCTCGCCCGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTGAAGTCATTCGCCGGCAAGCTGGTCAGGTGCATCGTCACATCCTGCTCGTCGTCCTCGAACTCATGCTCCTGCGCCACCTCGGCGaaa                         <  1:181353/148‑1 (MQ=255)

                                                                                                                               tGGCTCACACCCAGGCTCGCCCGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTGAAGTCATTCGCCGGCAAGCTGGTCAGGTGCATCGTCACATCCTGCTCGTCGTCCTCGAACTCATGCTCCTGCGCCACCTCGGCGAAAAg                       >  7:293341/1‑149 (MQ=255)

                                                                                                                                ggCTCACACCCAGGCTCGCCCGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTGAAGTCATTCGCCGGCAAGCTGGTCAGGTGCATCGTCACATCCTGCTCGTCGTCCTCGAACTCATGCTCCt                                          <  3:14040/129‑1 (MQ=255)

                                                                                                                                ggCTCACACCCAGGCTCGCCCGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTGAAGTCATTCGCCGGCAAGCTGGTCAGGTGCATCGTCACATCCTGCTCGTCGTCCTCGAACTCATGCTCCt                                          >  4:14040/1‑129 (MQ=255)

                                                                                                                                 gCTCACACCCAGGCTCGCCCGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTGAAGTCATTCGCCGGCAAGCTGGTCAGGTGCATCGTCACATCCTGCTCGTCGTCCTCGAACTCATGCTCCTGCGCCACCTCGGCGAAAAGcc                     <  5:139533/149‑1 (MQ=255)

                                                                                                                                  cTCACCCCCAGGCTCGCCCGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTGAAGTCATTCGCCGGCAAGCTGGTCAGGTGCATCGTCACATCCTGCTCGTCGTCCTCGAACTCATGCTCCTGCGCCACCTCGGCGAAAAGCCg                    >  6:325200/1‑149 (MQ=255)

                                                                                                                                  cTCACACCCAGGCTCGCCCGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTGAAGTCATTCGCCGGCAAGCTGGTCAGGTGCATCGTCACATCCTGCTCGTCGTCCTCGAACTCATGCTCCTGCGCCACCTCGGCGAAAAGCCg                    <  7:229531/149‑1 (MQ=255)

                                                                                                                                  cTCACACCCAGGCTCGCCCGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTGAAGTCATTCGCCGGCAAGCTGGTCAGGTGCATCGTCACATCCTGCTCGTCGTCCTCGAACTCATGCTCCTGCGCCACCTCGGCGAAAAGCCg                    <  6:137482/149‑1 (MQ=255)

                                                                                                                                   tCACACCCAGGCTCGCCCGGGACAGGTCGCTGTGCGGCGTATACCCGGACAGGGTGAAGTCATTCGCCGGCAAGCTGGTCAGGTGCATCGACACATCCTGCTCGTCGTCCTCGAACTCATGCTCCTGCGCCACCACGGCGAAACCCCgg                   >  8:337788/1‑149 (MQ=255)

                                                                                                                                     tgacCCGGGCGAGCCTGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTGAAGTCATTCGCCGGCAAGCTGGTCAGGTGCATCGTCACATCCTGCTCGTCGTCCTCGAACTCATGCTCCTGCGCCACCTCGGCGAAAAGCCgg                   >  6:134428/3‑147 (MQ=255)

                                                                                                                                     tgacCCGGGCGAGCCTGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTGAAGTCATTCGCCGGCAAGCTGGTCAGGTGCATCGTCACATCCTGCTCGTCGTCCTCGAACTCATGCTCCTGCGCCACCTCGGCGAAAAGCCgg                   <  5:134428/145‑1 (MQ=255)

                                                                                                                                     acacCCAGGCTCGCCCGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTGAAGTCATTCGCCGGCAAGCTGGTCAGGTGCATCGTCACATCCTGCTCGTCGTCCTCGAACTCATGCTCCTGCGCCACCTCGGCGAAAAGCCgg                   <  2:78057/147‑1 (MQ=255)

                                                                                                                                     acacCCAGGCTCGCCCGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTGAAGTCATTCGCCGGCAAGCTGGTCAGGTGCATCGTCACATCCTGCTCGTCGTCCTCGAACTCATGCTCCTGCGCCACCTCGGCGAAAAGCCgg                   <  3:181618/147‑1 (MQ=255)

                                                                                                                                       acCCGGGCGAGCCTGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCATGGTGAAGTCATTCGCCGGCAAGCTGGTCAGGTGCATCGTCACATCCTGCTCGTCGTCCTCGAACTCATGCTCCTGCGCCACCTCGGCGAAAAGCCGGGTAc               >  1:160347/1‑149 (MQ=255)

                                                                                                                                       acCCGGGCGAGCCTGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTGAAGTCATTCGCCGGCAAGCTGGTCAGGTGCATCGTCACATCCTGCTCGTCGTCCTCGAACTCATGCTCCTGCGCCACCTCGGCGAAAAGCCGGGTAc               >  7:271718/1‑149 (MQ=255)

                                                                                                                                       acCCAGGCTCGCCCGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTGAAGTCATTCGCCGGCAAGCTGGTCAGGTGCATCGTCACATCCTGCTCGTCGTCCTc                                                        <  8:74177/108‑1 (MQ=255)

                                                                                                                                       acCCAGGCTCGCCCGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTGAAGTCATTCGCCGGCAAGCTGGTCAGGTGCATCGTCACATCCTGCTCGTCGTCCTc                                                        >  7:74177/1‑108 (MQ=255)

                                                                                                                                       acCCAGGCTCGCCCGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTGAAGTCATTCGCCGGCAAGCTGGTCAGGTGCATCGTCACATCCTGCTCGTCGTCCTGGAACTCATGCTCCTGCGCCACCTCGGCGAAAAGCCGGGTAc               >  5:322318/1‑149 (MQ=255)

                                                                                                                                       acCCAGGCTCGCCCGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTGAAGTCATTCGCCGGCAAGCTGGTCAGGTGCATCGTCACATCCTGCTCGTCGTCCTCGAACTCATGCTCCTGCGCCACCTCGGCGAAAAGCCGGGTAc               >  1:290153/1‑149 (MQ=255)

                                                                                                                                        cccAGGCTCGCCCGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTGAAGTCATTCGCCGGCAAGCTGGTCAGGTGCATCGTCACATCCTGCTCGTCGTCCTCGAACTCATGCTCCTGCGCCACCTCGGCGAAAAGCCGGGTACt              <  7:195806/149‑1 (MQ=255)

                                                                                                                                         ccAGGCTCGCCCGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTGAAGTCATTCGCCGGCAAGCTGGTCAGGTGCATCGTCACATCCTGCTCGTCGTCCTCGAACTCATGCTCCTGCGCCACCTCGGCGAAAAGCCGGGTACtt             >  4:132238/1‑149 (MQ=255)

                                                                                                                                          cAGGCTCGCCCGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTGAAGTCATTCGCCGGCAAGCTGGTCAGGTGCATCGTCACATCCTGCTCGTCGTCCTCGAACTCATGCTCCTGCGCCACCTCGGCGAAAAGCCGGGTACTTg            >  6:226109/1‑149 (MQ=255)

                                                                                                                                          cAGGCTCGCCCGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTGAAGTCATTCGCCGGCAAGCTGGTCAGGTGCATCGTCACATCCTGCTCGTCGTCCTCGAACTCATGCTCCTGCGCCACCTCGGCGAAAAGCCGGGTACTTg            >  5:68853/1‑149 (MQ=255)

                                                                                                                                           aGGCTCGCCCGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTGAAGTCATTCGCCGGCAAGCTGGTCAGGTGCATCGTCACATCCTGCTCGTCGTCCTCGAACTCATGCTCCTGCGCCACCTCGGCGAAAAGCCGGGTACTTgg           >  3:212921/1‑149 (MQ=255)

                                                                                                                                           aGGCTCGCCCGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTGAAGTCATTCGCCGGCAAGCTGGTCAGGTGCATCGTCACATCCTGCTCGTCGTCCTCGAACTCATGCTCCTGCGCCACCTCGGCGAAAAGCCGGGTACTTgg           <  1:186033/149‑1 (MQ=255)

                                                                                                                                              cTCGCCCGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTGAAGTCATTCGCCGGCAAGCTGGTCAGGTGCATCGTCACATCCTGCTCGTCGTCCTCGAACTCATGCTCCTGCGCCACCTCGGCGAAAAGCCGGGTACTTGGCAg        <  1:147290/149‑1 (MQ=255)

                                                                                                                                                  cccGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTGAAGTCATTCGCCGGCAAGCTGGTCAGGTGCATCGTCACATCCTGCTCGTCGTCCTCGAACTCATGCTCCTGCGCCACCTCGGCGaaaa                        >  8:239859/1‑129 (MQ=255)

                                                                                                                                                  cccGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTGAAGTCATTCGCCGGCAAGCTGGTCAGGTGCATCGTCACATCCTGCTCGTCGTCCTCGAACTCATGCTCCTGCGCCACCTCGGCGaaaa                        <  7:239859/129‑1 (MQ=255)

                                                                                                                                                    cGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTGAAGTCATTCGCCGGCAAGCTGGTCAGGTGCATCGTCACATCCTGCTCGTCGTCCTCGAACTCATGCTCCTGCGCCACCTCGGCGAAAAGCCGGGTACTTGGCAGTACCTg  <  3:250004/149‑1 (MQ=255)

GGCCGCAAAGCGGCCCCCAGCAATTCAGAAGTCCACGCTAACCCCCACGCTAATACCCTGTTGCGTCAACTCATCACTCTTGCGCCAGTTGTAGTTCCCGCGCAAATGCACCCCTGCCACCAGTTCATGGCTCACACCCAGGCTCGCCCGGGTCAGGTCGCTGTGCGGCGTATACCCGGTCAGGGTGAAGTCATTCGCCGGCAAGCTGGTCAGGTGCATCGTCACATCCTGCTCGTCGTCCTCGAACTCATGCTCCTGCGCCACCTCGGCGAAAAGCCGGGTACTTGGCAGTACCTG  >  NC_002947/507097‑507393

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`