Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,019,077 G→T 6.6% A41A (GCC→GCA)  dadX ← alanine racemase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,019,0770GT 6.6% 283.7 / 6.2 88A41A (GCC→GCA) dadXalanine racemase
Reads supporting (aligned to +/- strand):  ref base G (40/42);  new base T (3/3);  total (43/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.56e-01

GATCAGCGCCAGTTCGCTGGCTTCAAAAAAACCTTCCAGCAGCAGTACCGGGGCTTTGATACCGGCGGCGCGCAGTTCCAGCGCCTCTTCGATGCAGGCCACGGCAAAGCCGTCGGCTTCGGCCTCGAGGGCCAGGGCGCAGCGCACGGCGCCGTGGCCGTAGGCATCGGCCTTGATCACGGCGAGGGCTTTGGCACCCGTCAGTTCACGGGCCAGACGGTAGTTGTGGCGCAGGGCCTGGAGGTCGATCAGGGCGCGGGCGGGACGCATAGCGGCTGCCTTATGGTGGTTCGGGT  >  NC_002947/6018930‑6019225
                                                                                                                                                   |                                                                                                                                                    
gATCAGCGCCAGTTCGCTGGCTTCAAAAAAACCTTCCAGCAGCAGTACCGGGGCTTTGATACCGGCGGCGCGCAGTTCCAGCGCCTCTTCGATGCAGGCCACGGCAAAGCCGTCGGCTTCGGCCTCGAGGGCCAGGGCGCAGCGCACgg                                                                                                                                                     >  5:117520/1‑149 (MQ=255)
    aGCGCCAGTTCGCTGGCTTCAAAAAAACCTTCCAGCAGCAGTACCGGGGCTTTGATACCGGCGGCGCGCAGTTCCAGCGCCTCTTCGATGCAGGCCACGGCAAAGCCGTCGGCTTCGGCCTCGAGGGCCAGGGCGCAGCGCACGGCGcc                                                                                                                                                 <  2:337274/149‑1 (MQ=255)
     gcgcCAGTTCGCTGGCTTCAAAAAAACCTTCCAGCAGCAGTACCGGGGCTTTGATACCGGCGGCGCGCAGTTCCAGCGCCTCTTCGATGCAGGCCACGGCAAAGCCGTCGGCTTCGGCCTCGAGGGCCAGGGCGCAGCGCACGGCGCCg                                                                                                                                                >  4:63933/1‑149 (MQ=255)
      cgcCAGTTCGCTGGCTTCAAAAAAACCTTCCAGCAGCAGTACCGGGGCTTTGATACCGGCGGCGCGCAGTTCCAGCGCCTCTTCGATGCAGGCCACGGCAAAGCCGTCGGCTTCGGCCTCGAGGGCCAGGGCGCAGCGCACGgc                                                                                                                                                    <  7:23904/144‑1 (MQ=255)
      cgcCAGTTCGCTGGCTTCAAAAAAACCTTCCAGCAGCAGTACCGGGGCTTTGATACCGGCGGCGCGCAGTTCCAGCGCCTCTTCGATGCAGGCCACGGCAAAGCCGTCGGCTTCGGCCTCGAGGGCCAGGGCGCAGCGCACGgc                                                                                                                                                    >  8:29506/1‑144 (MQ=255)
      cgcCAGTTCGCTGGCTTCAAAAAAACCTTCCAGCAGCAGTACCGGGGCTTTGATACCGGCGGCGCGCAGTTCCAGCGCCTCTTCGATGCAGGCCACGGCAAAGCCGTCGGCTTCGGCCTCGAGGGCCAGGGCGCAGCGCACGgc                                                                                                                                                    <  7:29506/144‑1 (MQ=255)
      cgcCAGTTCGCTGGCTTCAAAAAAACCTTCCAGCAGCAGTACCGGGGCTTTGATACCGGCGGCGCGCAGTTCCAGCGCCTCTTCGATGCAGGCCACGGCAAAGCCGTCGGCTTCGGCCTCGAGGGCCAGGGCGCAGCGCACGgc                                                                                                                                                    >  8:23904/1‑144 (MQ=255)
       gcCAGTTCGCTGGCTTCAAAAAAACCTTCCAGCAGCAGTACCGGGGCTTTGATACCGGCGGCGCGCAGTTCCAGCGCCTCTTCGATGCAGGCCACGGCAAAGCCGTCGGCTTCGGCCTCGAGGGCCAGGGCGCAGCGCACGGCGCCGTg                                                                                                                                              <  4:307211/149‑1 (MQ=255)
          aGTTCGCTGGCTTCAAAAAAACCTTCCAGCAGCAGTACCGGGGCTTTGATACCGGCGGCGCGCAGTTCCAGCGCCTCTTCGATGCAGGCCACGGCAAAGCCGTCGGCTTCGGCCTCGAGGGCCAGGGCGCAGCGCACGGCGCCGTGGcc                                                                                                                                           <  8:115507/149‑1 (MQ=255)
               gCTGGCTTCAAAAAAACCTTCCAGCAGCAGTACCGGGGCTTTGATACCGGCGGCGCGCAGTTCCAGCGCCTCTTCGATGCAGGCCACGGCAAAGCCGTCGGCTTCGGCCTCGAGGGCCAGGGCGCAGCGCACGGCGCCGTGGCCGTAgg                                                                                                                                      >  8:181233/1‑149 (MQ=255)
               gCTGGCTTCAAAAAAACCTTCCAGCAGCAGTACCGGGGCTTTGATACCGGCGGCGCGCAGTTCCAGCGCCTCTTCGATGCAGGCCACGGCAAAGCCGTCGGCTTCGGCCTCGAGGGCCAGGGCGCAGCGCACGGCGCCGTGGCCGTAgg                                                                                                                                      <  2:221942/149‑1 (MQ=255)
               gCTGGCTTCAAAAAAACCTTCCAGCAGCAGTACCGGGGCTTTGATACCGGCGGCGCGCAGTTCCAGCGCCTCTTCGATGCAGGCCACGGCAAAGCCGTCGGCTTCGGCCTCGAGGGCCAGGGCGCAGCGCACGGCGCCGTGGCCGTAg                                                                                                                                       <  6:210628/148‑1 (MQ=255)
                  ggCTTCAAAAAAACCTTCCAGCAGCAGTACCGGGGCTTTGATACCGGCGGCGCGCAGTTCCAGCGCCTCTTCGATGCAGGCCACGGCAAAGCCGTCGGCTTCGGCCTCGAGGGCCAGGGCGCAGCGCACGGCGCCGTGGCCGTAGGCAt                                                                                                                                   >  7:127319/1‑149 (MQ=255)
                     ttCAAAAAAACCTTCCAGCAGCAGTACCGGGGCTTTGATACCGGCGGCGCGCAGTTCCAGCGCCTCTTCGATGCAGGCCACGGCAAAGCCGTCGGCTTCGGCCTCGAGGGCCAGGGCGCAGCGCACGGCGCCGTGGCCGTAGGCATCgg                                                                                                                                >  5:91194/1‑149 (MQ=255)
                     ttCAAAAAAACCTTCCAGCAGCAGTACCGGGGCTTTGATACCGGCGGCGCGCAGTTCCAGCGCCTCTTCGATGCAGGCCACGGCAAAGCCGTCGGCTTCGGCCTCGAGGGCCAGGGCGCAGCGCACGGCGCCGTGGCCGTAGGCATCgg                                                                                                                                >  6:129160/1‑149 (MQ=255)
                       cAAAAAAACCTTCCAGCAGCAGTACCGGGGCTTTGATACCGGCGGCGCGCAGTTCCAGCGCCTCTTCGATGCAGGCCACGGCAAAGCCGTCGGCTTCGGCCTCGAGGGCCAGGGCGCAGCGCACGGCGCCGTGGCCGTAGGCATCGGc                                                                                                                               <  6:117520/148‑1 (MQ=255)
                        aaaaaaaCCTTCCAGCAGCAGTACCGGGGCTTTGATACCGGCGGCGCGCAGTTCCAGCGCCTCTTCGATGCAGGCCACGGCAAAGCCGTCGGCTTCGGCCTCGAGGGCCAGGGCGCAGCGCACGGCGCCGTGGCCGTAGGCATCGGCCt                                                                                                                             >  8:104599/1‑149 (MQ=255)
                         aaaaaaCCTTCCAGCAGCAGTACCGGGGCTTTGATACCGGCGGCGCGCAGTTCCAGCGCCTCTTCGATGCAGGCCACGGCAAAGCCGTCGGCTTCGGCCTCGAGGGCCAGGGCGCAGCGCACGGCGCCGTGGCCGTAGCCATCGGCCtt                                                                                                                            >  7:128982/1‑149 (MQ=255)
                          aaaaaCCTTCCAGCAGCAGTACCGGGGCTTTGATACCGGCGGCGCGCAGTTCCAGCGCCTCTTCGATACAGGCCACGGCAAAGCCGTCGGCTTCGGCCTCGAGGGCCAGGGCGCAGCGCACGGCGCCGTGGCCGTAGGCATCGGCCtt                                                                                                                            >  8:106501/1‑148 (MQ=255)
                            aaaCCTTCCAGCAGCAGTACCGGGGCTTTGATACCGGCGGCGCGCAGTTCCAGCGCCTCTTCGATGCAGGCCACGGCAAAGCCGTCGGCTTCGGCCTCGAGGGCCAGGGCGCAGCGCACGGCGCCGTGGCCGTAGGCATCGGCCTTGAt                                                                                                                         <  3:41114/149‑1 (MQ=255)
                              aCCTTCCAGCAGCAGTACCGGGGCTTTGATACCGGCGGCGCGCAGTTCCAGCGCCTCTTCGATGCAGGCCACGGCAAAGCCGTCGGCTTCGGCCTCGAGGGCCAGGGCGCAGCGCACGGCGCCGTGGCCGTAGGCATCGGCCTTGATCa                                                                                                                       >  2:316556/1‑149 (MQ=255)
                                   ccAGCAGCAGTACCGGGGCTTTGATACCGGCGGCGCGCAGTTCCAGCGCCTCTTCGATGCAGGCCACGGCAAAGCCGTCGGCTTCGGCCTCGAGGGCCAGGGCGCAGCGCACGGCGCCGTGGCCGTAGGCATCGGCCTTGATCACGGc                                                                                                                   <  5:16469/148‑1 (MQ=255)
                                     agcagcagTACCGGGGCTTTGATACCGGCGGCGCGCAGTTCCAGCGCCTCTTCGATGCAGGCCACGGCAAAGCCGTCGGCTTCGGCCTCGAGGGCCAGGGCGCAGCGCACGGCGCCGTGGCCGTAGGCATCGGcc                                                                                                                              <  5:95509/135‑1 (MQ=255)
                                     agcagcagTACCGGGGCTTTGATACCGGCGGCGCGCAGTTCCAGCGCCTCTTCGATGCAGGCCACGGCAAAGCCGTCGGCTTCGGCCTCGAGGGCCAGGGCGCAGCGCACGGCGCCGTGGCCGTAGGCATCGGcc                                                                                                                              >  6:95509/1‑135 (MQ=255)
                                      gcagcagTACCGGGGCTTTGATACCGGCGGCGCGCAGTTCCAGCGCCTCTTCGATGCAGGCCACGGCAAAGCCGTCGGCTTCGGCCTCGAGGGCCAGGGCGCAGCGCACGGCGCCGTGGCCGTAGGCATCGGcc                                                                                                                              <  2:177312/134‑1 (MQ=255)
                                      gcagcagTACCGGGGCTTTGATACCGGCGGCGCGCAGTTCCAGCGCCTCTTCGATGCAGGCCACGGCAAAGCCGTCGGCTTCGGCCTCGAGGGCCAGGGCGCAGCGCACGGCGCCGTGGCCGTAGGCATCGGcc                                                                                                                              >  1:177312/1‑134 (MQ=255)
                                      gcagcagTACCGGGGCTTTGATACCGGCGGCGCGCAGTTCCAGCGCCTCTTCGATGCAGGCCACGGCAAAGCCGTCGGCTTCGGCCTCGAGGGCCAGGGCGCAGCGCACGGCGCCGTGGCCGTAGGCATCGGCCTTGATCACGGCGAgg                                                                                                               >  5:364433/1‑149 (MQ=255)
                                       cagcagTACCGGGGCTTTGATACCGGCGGCGCGCAGTTCCAGCGCCTCTTCGATGCAGGCCACGGCAAAGCCGTCGGCTTCGGCCTCGAGGGCCACGGCGCCGTGCGCTGCGCCCTGGccctcgag                                                                                                                                     >  4:152649/1‑120 (MQ=37)
                                       cagcagTACCGGGGCTTTGATACCGGCGGCGCGCAGTTCCAGCGCCTCTTCGATGCAGGCCACGGCAAAGCCGTCGGCTTCGGCCTCGAGGGCCACGGCGCCGTGCGCTGCGCCCTGGccctcgag                                                                                                                                     <  3:152649/126‑7 (MQ=37)
                                       cagcagTACCGGGGCTTTGATACCGGCGGCGCGCAGTTCCAGCGCCTCTTCGATGCAGGCCACGGCAAAGCCGTCGGCTTCGGCCTCGAGGGCCACGGCGCCGTGCGCTGCGCCCTGGcc                                                                                                                                           <  7:266885/120‑1 (MQ=38)
                                       cagcagTACCGGGGCTTTGATACCGGCGGCGCGCAGTTCCAGCGCCTCTTCGATGCAGGCCACGGCAAAGCCGTCGGCTTCGGCCTCGAGGGCCACGGCGCCGTGCGCTGCGCCCTGGcc                                                                                                                                           >  8:266885/1‑120 (MQ=38)
                                            gccccGGGGCTTTGATACCGGCGGCGCGCAGTTCCAGCGCCTCTTCGATGCAGGCCACGGCAAAGCCGTCGGCTTCGGCCTCGAGGGCCAGGGCGCAGCGCACGGCGCCGTGGCCGTAGGCATCGGCCTTGATCACGGCGAGGGCTTTg                                                                                                         <  1:316556/146‑1 (MQ=255)
                                            gTACCGGGGCTTTGATACCGGCGGCGCGCAGTTCCAGCGCCTCTTCGATGCAGGCCACGGCAAAGCCGTCGGCTTCGGCCTCGAGGGCCAGGGCGCAGCGCACGGCGCCGTGGCCGTAGGCATCGGCCTTGATCACGGCGAGGGCTTTg                                                                                                         <  3:233257/149‑1 (MQ=255)
                                            gTACCGGGGCTTTGATACCGGCGGCGCGCAGTTCCAGCGCCTCTTCGATGCAGGCCACGGCAAAGCCGTCGGCTTCGGCCTCGAGGGCCAGGGCGCAGCGCACGGCGCCGTGGCCGTAGGCATCGGCCTTGATCACGGCGAGGGCTTTg                                                                                                         <  3:209808/149‑1 (MQ=255)
                                               ccGGGGCTTTGATACCGGCGGCGCGCAGTTCCAGCGCCTCTTCGATGCAGGCCACGGCAAAGCCGTCGGCTTCGGCCTCGAGGGCCAGGGCGCAGCGCACGGCGCCGTGGCCGTAGGCATCGGcc                                                                                                                              <  2:185102/125‑1 (MQ=255)
                                               ccGGGGCTTTGATACCGGCGGCGCGCAGTTCCAGCGCCTCTTCGATGCAGGCCACGGCAAAGCCGTCGGCTTCGGCCTCGAGGGCCAGGGCGCAGCGCACGGCGCCGTGGCCGTAGGCATCGGcc                                                                                                                              >  1:185102/1‑125 (MQ=255)
                                                cGGGGCTTTGATACCGGCGGCGCGCAGTTCCAGCGCCTCTTCGATGCAGGCCACGGCAAAGCCGTCGGCTTCGGCCTCGAGGGCCAGGGCGCAGCGCACGGCGCCGTGGCCGTAGGCATCGGCCTTGATCACGGCGAGGGCTTTGGCAc                                                                                                     >  2:17211/1‑149 (MQ=255)
                                                  gggCTTTGATACCGGCGGCGCGCAGTTCCAGCGCCTCTTCGATGCAGGCCACGGCAAAGCCGTCGGCTTCGGCCTCGAGGGCCAGGGCGCAGCGCACGGCGCCGTGGCCGTAGGCATCGGCCTTGATCACGGCGAGGGCtt                                                                                                           >  6:242255/1‑141 (MQ=255)
                                                  gggCTTTGATACCGGCGGCGCGCAGTTCCAGCGCCTCTTCGATGCAGGCCACGGCAAAGCCGTCGGCTTCGGCCTCGAGGGCCAGGGCGCAGCGCACGGCGCCGTGGCCGTAGGCATCGGCCTTGATCACGGCGAGGGCtt                                                                                                           <  5:242255/141‑1 (MQ=255)
                                                      tttGATACCGGCGGCGCGCAGTTCCAGCGCCTCTTCGATACAGGCCACGGCAAAGCCGTCGGCTTCGGCCTCGAGGGCCAGGGCGCAGCGCACGGCGCCGTGGCCGTAGGCATCGGCCTTGATCACGGCGAGGGCTTTGGCACCCGTCa                                                                                               <  7:106501/149‑1 (MQ=255)
                                                         gATACCGGCGGCGCGCAGTTCCAGCGCCTCTTCGATGCAGGCCACGGCAAAGCCGTCGGCTTCGGCCTCGAGGGCCAGGGCGCAGCGCACGGCGCCGTGGCCGTAGGCATCGGCCTTGATCACGGCGAGGGCtt                                                                                                           >  7:75262/1‑134 (MQ=255)
                                                         gATACCGGCGGCGCGCAGTTCCAGCGCCTCTTCGATGCAGGCCACGGCAAAGCCGTCGGCTTCGGCCTCGAGGGCCAGGGCGCAGCGCACGGCGCCGTGGCCGTAGGCATCGGCCTTGATCACGGCGAGGGCtt                                                                                                           <  8:75262/134‑1 (MQ=255)
                                                          aTACCGGCGGCGCGCAGTTCCAGCGCCTCTTCGATGCAGGCCACGGCAAAGCCGTCGGCTTCGGCCTCGAGGGCCAGGGCGCAGCGCACGGCGCCGTGGCCGTAGGCATCGGCCTTGATCACGGCGAGGGCTTTGGCACCCGTCAGtt                                                                                            >  4:247831/1‑148 (MQ=255)
                                                              cggcggCGCGCAGTTCCAGCGCCTCTTCGATGCAGGCCACGGCAAAGCCGTCGGCTTCGGCCTCGAGGGCCAGGGCGCAGCGCACGGCGCCGTGGCCGTAGGCATCGGCCTTGATCACGGCGAGGGCTTTGGCACCCGTCAGTTCACgg                                                                                       >  6:275695/1‑149 (MQ=255)
                                                              cggcggCGCGCAGTTCCAGCGCCTCTTCGATGCAGGCCACGGCAAAGCCGTCGGCTTCGGCCTCGAGGGCCAGGGCGCAGCGCACGGCGCCGTGGCCGTAGGCATCGGCCTTGATCACGGCGAGGGCTTTGGCACCCGTCAGTTCACgg                                                                                       <  8:286011/149‑1 (MQ=255)
                                                                 cggcgCGCAGTTCCAGCGCCTCTTCGATGCAGGCCACGGCAAAGCCGTCGGCTTCGGCCTCGAGGGCCAGGGCGCAGCGCACGGCGCCGTGGCCGTAGGCATCGGCCTTGATCa                                                                                                                       >  1:153003/1‑114 (MQ=255)
                                                                 cggcgCGCAGTTCCAGCGCCTCTTCGATGCAGGCCACGGCAAAGCCGTCGGCTTCGGCCTCGAGGGCCAGGGCGCAGCGCACGGCGCCGTGGCCGTAGGCATCGGCCTTGATCa                                                                                                                       <  2:153003/114‑1 (MQ=255)
                                                                         aGTTCCAGCGCCTCTTCGATGCAGGCCACGGCAAAGCCGTCGGCTTCGGCCTCGAGGGCCAGGGCGCAGCGCACGGCGCCGTGGCCGTAGGCATCGGCCTTGATCACGGCGAGGGCTTTGGCACCCGTCAGTTCACGGGCCAGACGGTa                                                                            <  5:129160/149‑1 (MQ=255)
                                                                          gTTCCAGCGCCTCTTCGATGCAGGCCACGGCAAAGCCGTCGGCTTCGGCCTCGAGGGCCAGGGCGCAGCGCACGGCGCCGTGGCCGTAGGCATCGGCCTTGATCACGGCGAGGGCTTTGGCACCCGTCAGTTCACGGGCCAGACGGTAg                                                                           <  3:63933/149‑1 (MQ=255)
                                                                             ccAGCGCCTCTTCGATGCAGGCCACGGCAAAGCCGTCGGCTTCGGCCTCGAGGGCCAGGGCGCAGCGCACGGCGCCGTGGCCGTAGGCATCGGCCTTGATCACGGCGAGGGCTTTGGCACCCGTCAGTTCACGGGCCAGACGGTAGTtg                                                                        >  5:302417/1‑149 (MQ=255)
                                                                                   ccTCTTCGATGCAGGCCACGGCAAAGCCGTCGGCTTCGGCCTCGAGGGCCAGGGCGCAGCGCACGGCGCCGTGGCCGTAGGCATCGGCCTTGATCACGGCGAGGGCTTTGGCACCCGTCAGTTCACg                                                                                        <  5:6604/127‑1 (MQ=255)
                                                                                   ccTCTTCGATGCAGGCCACGGCAAAGCCGTCGGCTTCGGCCTCGAGGGCCAGGGCGCAGCGCACGGCGCCGTGGCCGTAGGCATCGGCCTTGATCACGGCGAGGGCTTTGGCACCCGTCAGTTCACg                                                                                        >  6:6604/1‑127 (MQ=255)
                                                                                   ccTCTTCGATGCAGGCCACGGCAAAGCCGTCGGCTTCGGCCTCGAGGGCCAGGGCGCAGCGCACGGCGCCGTGGCCGTAGGCATCGGCCTTGATCACGGCGAGGGCTTTGGCACCCGTCAGTTCACGGGCCAGACGGTAGTTGTGgcg                                                                   >  6:284789/1‑148 (MQ=255)
                                                                                     tctTCGATGCAGGCCACGGCAAAGCCGTCGGCTTCGGCCTCGAGGGCCAGGGCGCAGCGCACGGCGCCGTGGCCGTAGGCATCGGCCTTGATCACGGCGAGGGCTTTgg                                                                                                        <  3:336448/109‑1 (MQ=255)
                                                                                     tctTCGATGCAGGCCACGGCAAAGCCGTCGGCTTCGGCCTCGAGGGCCAGGGCGCAGCGCACGGCGCCGTGGCCGTAGGCATCGGCCTTGATCACGGCGAGGGCTTTgg                                                                                                        >  4:336448/1‑109 (MQ=255)
                                                                                       ttCGATGCAGGCCACGGCAAAGCCGTCGGCTTCGGCCTCGAGGGCCAGGGCGCAGCGCACGGCGCCGTGGCCGTAGGCATCGGCCTTGATCACGGCGAGGGCTTTGGCACCCGTCAGTTCACGGGCCAGACGGTAGTTGTGGCGCAg                                                                <  6:364433/147‑1 (MQ=255)
                                                                                              cAGGCCACGGCAAAGCCGTCGGCTTCGGCCTCGAGGGCCAGGGCGCAGCGCACGGCGCCGTGGCCGTAGGCATCGGCCTTGATCACGGCGAGGGCTTTGGCACCCGTCAGTTCACGGGCCAGACGGTAGTTGTGGCGCAGGGCCTGGa                                                        <  1:17211/148‑1 (MQ=255)
                                                                                              cAGGCCACGGCAAAGCCGTCGGCTTCGGCCTCGAGGGCCAGGGCGCAGCGCACGGCGCCGTGGCCGTAGGCATCGGCCTTGATCACGGCGAGGGCTTTGGCACCCGTCAGTTCACGGGCCAGACGGTAGTTGTGGCGCAGGGCCTGGAg                                                       <  8:127319/149‑1 (MQ=255)
                                                                                               aGGCCACGGCAAAGCCGTCGGCTTCGGCCTCGAGGGCCAGGGCGCAGCGCACGGCGCCGTGGCCGTAGGCATCGGCCTTGATCACGGCGAGGGCTTTGGCACCCGTCAGTTCACGGGCCAGACGGTAGTTGTGGCGCAGGGCCTGGAgg                                                      >  1:301929/1‑149 (MQ=255)
                                                                                                 gCCACGGCAAAGCCGTCGGCTTCGGCCTCGAGGGCCAGGGCGCAGCGCACGGCGCCGTGGCCGTAGGCATCGGCCTTGATCACGGCGAGGGCTTTGGCACCCGTCAGTTCACGGGCCAGACGGTAGTTGTGGCGCAGGGCCTGGAGGt                                                     <  6:302417/148‑1 (MQ=255)
                                                                                                   cACGGCAAAGCCGTCGGCTTCGGCCTCGAGGGCCAGGGCGCAGCGCACGGCGCCGTGGCCGTAGGCATCGGCCTTGATCACGGCGAGGGCTTTGGCACCCGTCAGTTCACGGGCCAGACGGTAGTTGTGGCGCAGGGCCTGGAGGTCGa                                                  >  4:233952/1‑149 (MQ=255)
                                                                                                   cACGGCAAAGCCGTCGGCTTCGGCCTCGAGGGCCAGGGCGCAGCGCACGGCGCCGTGGCCGTAGGCATCGGCCTTGATCACGGCGAGGGCTTTGGCACCCGTCAGTTCACGGGCCAGACGGTAGTTGTGGCGCAGGGCCTGGAGGTCGa                                                  >  7:76013/1‑149 (MQ=255)
                                                                                                    aCGGCAAAGCCGTCGGCTTCGGCCTCGAGGGCCAGGGCGCAGCGCACGGCGCCGTGGCCGTAGGCATCGGCCTTGATCACGGCGAGGGCTTTGGCACCCGTCAGTTCACGGGCCAGACGGTAGTTGTGGCGCAGGGCCTGGAGGTc                                                    >  8:232156/1‑146 (MQ=255)
                                                                                                    aCGGCAAAGCCGTCGGCTTCGGCCTCGAGGGCCAGGGCGCAGCGCACGGCGCCGTGGCCGTAGGCATCGGCCTTGATCACGGCGAGGGCTTTGGCACCCGTCAGTTCACGGGCCAGACGGTAGTTGTGGCGCAGGGCCTGGAGGTCGAt                                                 >  4:289872/1‑149 (MQ=255)
                                                                                                        cAAAGCCGTCGGCTTCGGCCTCGAGGGCCAGGGCGCAGCGCACGGCGCCGTGGCCGTAGGCATCGGCCTTGATCACGGCGAGGGCTTTGGCACCCGTCAGTTCACGGGCCAGACGGTAGTTGTGGCGCAGGGCCTGGAGGTCGATCAgg                                             >  3:176696/1‑149 (MQ=255)
                                                                                                         aaaGCCGTCGGCTTCGGCCTCGAGGGCCAGGGCGCAGCGCACGGCGCCGTGGCCGTAGGCATCGGCCTTGATCACGGCGAGGGCTTTGGCACCCGTCAGTTCACGGGCCAGACGGTAGTTGTGGCGCAGGGCCTGGAGGTCGATc                                                >  5:326058/1‑145 (MQ=255)
                                                                                                         aaaGCCGTCGGCTTCGGCCTCGAGGGCCAGGGCGCAGCGCACGGCGCCGTGGCCGTAGGCATCGGCCTTGATCACGGCGAGGGCTTTGGCACCCGTCAGTTCACGGGCCAGACGGTAGTTGTGGCGCAGGGCCTGGAGGTCGATc                                                <  6:326058/145‑1 (MQ=255)
                                                                                                              cGTCGGCTTCGGCCTCGAGGGCGAGGGCGCAGCGCACGGCGCCGTGGCGGTAGGCATCGGCCTTGATCACGGCGAGGGCTTTGGCACCCGTCAGTTCACGGGCCAGACGGTAGTTGTGGCGCAGGGCCTGGAGGTCGATCAGGGCgcgg                                       <  5:127963/149‑1 (MQ=255)
                                                                                                                  ggCTTCGGCCTCGAGGGCCAGGGCGCAGCGCACGGCGCCGTGGCCGTAGGCATCGGCCTTGATCACGGCGAGGGCTTTGGCACCCGTCAGTTCACGGGCCAGACGGTAGTTGTGGCGCAGGGCCTGGAGGTCGATCAGGGCgcgggcgg                                   >  3:37303/1‑149 (MQ=255)
                                                                                                                   gCTTCGGCCTCGAGGGCCAGGGCGCAGCGCACGGCGCCGTGGCCGTAGGCATCGGCCTTGATCACGGCGAGGGCTTTGGCACCCGTCAGTTCACGGGCCAGACGGTAGTTGTGGCGCAGGGCCTGGAGGTCGATCAGGGCgcgggcgg                                   >  7:309399/1‑148 (MQ=255)
                                                                                                                   gCTTCGGCCTCGAGGGCCAGGGCGCAGCGCACGGCGCCGTGGCCGTAGGCATCGGCCTTGATCACGGCGAGGGCTTTGGCACCCGTCAGTTCACGGGCCAGACGGTAGTTGTGGCGCAGGGCCTGGAGGTCGATCAGGGCGcgggcggg                                  <  3:233952/149‑1 (MQ=255)
                                                                                                                    cTTCGGCCTCGAGGGCCAGGGCGCAGCGCACGGCGCCGTGGCCGTAGGCATCGGCCTTGATCACGGCGAGGGCTTTGGCACCCGTCAGTTCACGGGCCAGACGGTAGTTGTGGCGCAGGGCCTGGAGGTCGATCAGGGCGCGGGCGGGa                                 <  7:104599/149‑1 (MQ=255)
                                                                                                                     ttCGGCCTCGAGGGCCAGGGCGCAGCGCACGGCGCCGTGGCCGTAGGCATCGGCCTTGATCACGGCGAGGGCTTTGGCACCCGTCAGTTCACGGGCCAGACGGTAGTTGTGGCGCAGGGCCTGGAGGTCGATCAGGGCGCGGGCGGGAc                                <  7:181233/149‑1 (MQ=255)
                                                                                                                          ccTCGAGGGCCAGGGCGCAGCGCACGGCGCCGTGGCCGTAGGCATCGGCCTTGATCACGGCGAGGGCTTTGGCACCCGTCAGTTCACGGGCCAGACGGTAGTTGTGGCGCAGGGCCTGGAGGTCGATCAGGGCGCGGGCGGGACGCATa                           <  4:37303/149‑1 (MQ=255)
                                                                                                                           cTCGAGGGCCAGGGCGCAGCGCACGGCGCCGTGGCCGTAGGCATCGGCCTTGATCACGGCGAg                                                                                                                <  7:2664/63‑1 (MQ=255)
                                                                                                                           cTCGAGGGCCAGGGCGCAGCGCACGGCGCCGTGGCCGTAGGCATCGGCCTTGATCACGGCGAg                                                                                                                >  8:2664/1‑63 (MQ=255)
                                                                                                                           cTCGAGGGCCAGGGCGCAGCGCACGGCGCCGTGGCCGTAGGCATCGGCCTTGATCACGGCGAGGGCTTTGGCACCCGTCAGTTCACGGGCCAGACGGTAGTTGTGGCGCAGGGCCTGGAGGTCGATCAGGGCGCGGGCGGGACGCATAg                          >  6:5398/1‑149 (MQ=255)
                                                                                                                           cTCGAGGGCCAGGGCGCAGCGCACGGCGCCGTGGCCGTAGGCATCGGCATTGATCACGGCGAGGGCTTTGGCACCCGTCAGTTCACGGGCCAGACGGTAGTTGTGGCGCAGGGCCTGGAGGTCGATCAGGGCGCGGGCGGGACGCATAg                          >  1:85904/1‑149 (MQ=255)
                                                                                                                             ctacggCCACGGCGCCGTGCGCTGCGCCCTGGCCGTAGGCATCGGCCTTGATCACGGCGAGGGCTTTGGCACCCGTCAGTTCACGGGCCAGACGGTAGTTGTGGCGCAGGGCCTGGAGGTCGATCa                                               >  1:133568/5‑126 (MQ=38)
                                                                                                                             ctacggCCACGGCGCCGTGCGCTGCGCCCTGGCCGTAGGCATCGGCCTTGATCACGGCGAGGGCTTTGGCACCCGTCAGTTCACGGGCCAGACGGTAGTTGTGGCGCAGGGCCTGGAGGTCGATCa                                               <  2:133568/122‑1 (MQ=38)
                                                                                                                                 ggCCAGGGCGCAGCGCACGGCGCCGTGGCCGTAGGCATCGGCCTTGATCACGGCGAGGGCTTTGGCACCCGTCAGTTCACGGGCCAGACGGTAGTTGTGGCGCAGGGCCTGGAGGTCGATCAGGGCGCGGGCGGGACGCATAGCGGCt                     <  7:232156/148‑1 (MQ=255)
                                                                                                                                  gCCAGGGCGCAGCGCACGGCGCCGTGGCCGTAGGCATCGGCCTTGATCACGGCGAGGGCTTTGGCACCCGTCAGTTCACGGGCCAGACGGTAGTTGTGGCGCAGGGCCTGGAGGTCGATCAGGGCGCGGGCGGGACGCATAGCGGCTGc                   <  3:247831/149‑1 (MQ=255)
                                                                                                                                  gCCAGGGCGCAGCGAACGGCGCCGTGGCCGTAGGCATCGGCCTTGATCACGGCGAGGGCTTTGGCACCCGTCAGTTCACGGGACAGACGGTAGTTGTGGCGCAGGGCCTGGAGGTCGATCAGGGCGCGGGCGGCACGAATAGCGGCtca                   >  4:82467/1‑147 (MQ=255)
                                                                                                                                   ccAGGGCGCAGCGCACGGCGCCGTGGCCGTAGGCATCGGCCTTGATCACGGCGAGGGCTTTGGCACCCGTCAGTTCACGGGCCAGACGGTAGTTGTGGCGCAGGGCCTGGAGGTCGATCAGGGCGCGGGCGGGACGCATAGCGGCTGcc                  >  8:355888/1‑149 (MQ=255)
                                                                                                                                       ggCGCAGCGCACGGCGCCGTGGCCGTAGGCATCGGCCTTGATCACGGCGAGGGCTTTGGCACCCGTCAGTTCACGGGCCAGACGGTAGTTGTGGCGCAGGGCCTGGAGGTCGATCAGGGCGCGGGCGGGACGCATAGCGGCTGCCTTAt              <  5:275695/149‑1 (MQ=255)
                                                                                                                                         cgcagcgcaCGGCGCCGTGGCCGTAGGCATCGGCCTTGATCACGGCGAGGGCTTTGGCACCCGTCAGTTCACGGGCCAGACGGTAGTTGTGGCGCAGGGCCTGGAGGTCGATCAGGGCGCGGGCGGGACGCATAGCGGCTGCCTTAtgg            <  4:176696/149‑1 (MQ=255)
                                                                                                                                           cagcgcaCGGCGCCGTGGCCGTAGGCATCGGCCTTGATCACGGCGAGGGCTTTGGCACCCGTCAGTTCACGGGCCAGACGGTAGTTGTGGCGCAGGGCCTGGAGGTCGATCAGGGCGCGGGCGGGACGCATAGCGGCTGCCTTAtggtg          <  5:5398/149‑1 (MQ=255)
                                                                                                                                                 aCGGCGCCGTGGCCGTAGGCATCGGCCTTGATCACGGCGAGGGCTTTGGCACCCGTCAGTTCACGGGCCAGACGGTAGTTGTGGCGCAGGGc                                                             <  2:212332/92‑1 (MQ=255)
                                                                                                                                                 aCGGCGCCGTGGCCGTAGGCATCGGCCTTGATCACGGCGAGGGCTTTGGCACCCGTCAGTTCACGGGCCAGACGGTAGTTGTGGCGCAGGGc                                                             >  1:212332/1‑92 (MQ=255)
                                                                                                                                                   ggCGCCGTGGCCGTAGGCATCGGCCTTGATCACGGCGAGGGCTTTGGCACCCGTCAGTTCACGGGCCAGACGGTAGTTGTGGCGCAGGGCCTGGAGGTCGATCAGGGCGCGGGCGGGACGCATAGCGGCTGCCTTATGGTGGTTCGGGt  >  2:83682/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GATCAGCGCCAGTTCGCTGGCTTCAAAAAAACCTTCCAGCAGCAGTACCGGGGCTTTGATACCGGCGGCGCGCAGTTCCAGCGCCTCTTCGATGCAGGCCACGGCAAAGCCGTCGGCTTCGGCCTCGAGGGCCAGGGCGCAGCGCACGGCGCCGTGGCCGTAGGCATCGGCCTTGATCACGGCGAGGGCTTTGGCACCCGTCAGTTCACGGGCCAGACGGTAGTTGTGGCGCAGGGCCTGGAGGTCGATCAGGGCGCGGGCGGGACGCATAGCGGCTGCCTTATGGTGGTTCGGGT  >  NC_002947/6018930‑6019225

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑