Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,021,700 T→G 10.1% intergenic (‑51/‑93) PP_5272 ← / → PP_5273 hypothetical protein/oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,021,7000TG10.1% 128.9 / 8.2 50intergenic (‑51/‑93)PP_5272/PP_5273hypothetical protein/oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (22/23);  new base G (2/3);  total (24/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.98e-01

GGCCGCAGCAGTCGTGGCAGCCGGGCTCGCACTCGAAAGAGGGGATGAGTTCGCGCAGGAAGTGGATCTTGTGGCGGTTGCAGGACATGACGGGATACAAGCTGACAAAGTGATTGGATTATAGGCTTATTCGCGGGTTCACCCGCGAACGAGTATGAAGCCCTCGCTGGTTGCCCGGCCGCGATGGCACAGCTTATCCTCGTCCCCCCTCCATTTTTCAGGGCCGCCCCAATGATCCACAGCGCGCAGCACGCCGCCTCCTATTACGCCGCCAGCAGCGC  >  NC_002947/6021562‑6021842
                                                                                                                                          |                                                                                                                                              
ggCCGCAGCAGTCGTGGCAGCCGGGCTCGCACTCGAAAGAGGGGATGAGTTCGCGCAGGAAGTGGATCTTGTGGCGGTTGCAGGACATGACGGGATACAAGCTGACAAAGTGATTGGATTATAGGCTTATTCGCGGGTGAACCCGCGaa                                                                                                                                      <  7:212785/149‑1 (MQ=255)
 gCCGCAGCAGTCGTGGCAGGCGGGCGCGCACGAGAAAGAGGGGATGAGTTCGAGCAGGAAGTGGATCGTGTGGCGGTTGCAGGACATGACGGGATACAAGCTGACAAAGTGATTGGATTATAGGCTTATTCGCGGGTTCACCCGCGAAc                                                                                                                                     <  2:228360/149‑1 (MQ=255)
   cGCAGCAGTCGTGGCAGCCGGGCTCGCACTCGAAAGAGGGGATGAGTTCGCGCAGGAAGTGGATCTTGTGGCGGTTGCAGGACATGACGGGATACAAGCTGACAAAGTGATTGGATTATAGGCTTATTCGCGGGTGAACCCGCGaataa                                                                                                                                   <  4:229029/149‑4 (MQ=255)
     cagcagTCGTGGCAGCCGGGCTCGCACTCGAAAGAGGGGATGAGTTCGCGCAGGAAGTGGATCTTGTGGCGGTTGCAGGACATGACGGGATACAAGCTGACAAAGTGATTGGATTATAGGCTTATTCGCGGGTTCACCCGCGAACGAGt                                                                                                                                 <  2:213865/149‑1 (MQ=255)
     cagcagTCGTGGCAGCCGGGCTCGCACTCGAAAGAGGGGATGAGTTCGCGCAGGAAGTGGATCTTGTGGCGGTTGCAGGACATGACGGGATACAAGCTGACAAAGTGATTGGATTATAGGCTTATTCGCGGGTGAACCCGCGaataagc                                                                                                                                 <  4:86694/149‑6 (MQ=255)
        cagTCGTGGCAGCCGGGCTCGCACTCGAAAGAGGGGATGAGTTCGCGCAGGAAGTGGATCTTGTGGCGGTTGCAGGACATGACGGGATACAAGCTGACAAAGTGATTGGATTATAGGCTTATTCGCGGGTTCACCCGCGAACGAGTATg                                                                                                                              <  6:256334/149‑1 (MQ=255)
          gTCGTGGCAGCCGGGCTCGCACTCGAAAGAGGGGATGAGTTCGCGCAGGAAGTGGATCTTGTGGCGGTTGCAGGACATGACGGGATACAAGCTGACAAAGTGATTGGATTATAGGCTTATTCGCGGGTTCACCCGCGAACGAGTATGaa                                                                                                                            <  1:372361/149‑1 (MQ=255)
                tgtgacGGGCTCTCACTCGAAAGAGTGGTTTAGTGCGCGCTGTTATTGGATATTGTGGCGGTTGCAGGACATGACGGGATACAAGCTGACAAAGTGATTGGATTATAGGCTTATTCGCGGGTTCACCCGCGAACGAGTATGaa                                                                                                                            <  6:149272/138‑1 (MQ=255)
                gCAGCCGGGCTCGCACTCGAAAGAGGGGATGAGTTCGCGCAGGAAGTGGATCTTGTGGCGTTTGCAGGACATGACGGGATACTAGGTGACAAAGTGATTGGTTTATTGGCTTTTTCGCGGGTTCACCCGCGAACGAGTATGaa                                                                                                                            >  5:149272/1‑143 (MQ=255)
                  aGCCGGGCTCGCACTCGAAAGAGGGGATGAGTTCGCGCAGGAAGTGGATCTTGTGGCGGTTGCAGGACATGACGGGATACAAGCTGACAAAGTGATTGGATTATAGGCTTATTCGCGGGTTCACCCGCGAACGAGTATGAAGCCCTCGc                                                                                                                    >  6:223457/1‑149 (MQ=255)
                     cGGGCTCGCACTCGAAAGAGGGGATGAGTTCGCGCAGGAAGTGGATCTTGTGGCGGTTGCAGGACATGACGGGATACAAGCTGACAAAGTGATTGGATTATAGGCTTATTCGCGGGTTCACCCGCGAACGAGTATGAAGCCCTCGCTgg                                                                                                                 <  6:181237/149‑1 (MQ=255)
                                   aaaGAGGGGATGAGTTCGCGCAGGAAGTGGATCTTGTGGCGGTTGCAGGACATGACGGGATACAAGCTGACAAAGTGATTGGATTATAGGCTTATTCGCGGGTTCACCCGCGAACGAGTATGAAGCCCTCGCTGGTTGCCCGGCCGCGa                                                                                                   <  8:248017/149‑1 (MQ=255)
                                   aaaGAGGGGATGAGTTCGCGCAGGAAGTGGATCTTGTGGCGGTTGCAGGACATGACGGGATACAAGCTGACAAAGTGATTGGATTATAGGCTTATTCGCGGGTTCACCCGCGAACGAGTATGAAGCCCTCGCTGGTTGCCCGGCCGCGa                                                                                                   >  7:248017/1‑149 (MQ=255)
                                    aaGAGGGGATGAGTTCGCGCAGGAAGTGGATCTTGTGGCGGTTGCAGGACATGACGGGATACAAGCTGACAAAGTGATTGGATTATAGGCTTATTCGCGGGTTCACCCGCGAACGAGTATGAAGCCCTCGCTGGTTGCCCGGCCGCGAt                                                                                                  <  7:8511/149‑1 (MQ=255)
                                     agagGGGATGAGTTCGCGCAGGAAGTGGATCTTGTGGCGGTTGCAGGACATGACGGGATACAAGCTGACAAAGTGATTGGATTATAGGCTTATTCGCGGGTTCACCCGCGAACGAGTATGAAGCCCTCGCTGGTTGCCCGGCCGCGATg                                                                                                 <  4:185993/149‑1 (MQ=255)
                                       agGGGATGAGTTCGCGCAGGAAGTGGATCTTGTGGCGGTTGCAGGACATGACGGGATACAAGCTGACAAAGTGATTGGATTATAGGCTTATTCGCGGGTTCACCCGCGAACGAGTATGAAg                                                                                                                           >  6:241114/1‑121 (MQ=255)
                                       agGGGATGAGTTCGCGCAGGAAGTGGATCTTGTGGCGGTTGCAGGACATGACGGGATACAAGCTGACAAAGTGATTGGATTATAGGCTTATTCGCGGGTTCACCCGCGAACGAGTATGAAg                                                                                                                           <  5:241114/121‑1 (MQ=255)
                                          ggATGAGTTCGCGCAGGAAGTGGATCTTGTGGCGGTTGCAGGACATGACGGGATACAAGCTGACAAAGTGATTGGATTATAGGCTTATTCGCGGGTTCACCCGCGAACGAGTATGAAGCCCTCGCTGGTTGCCCGGCCGCGATGGcaca                                                                                            <  1:318850/149‑1 (MQ=255)
                                           gATGAGTTCGCGCAGGAAGTGGATCTTGTGGCGGTTGCAGGACATGACGGGATACAAGCTGCCAAAGTGATTGGATTATAGGCTTATTCGCGGGTTCACCCGCGAACGAGTATGAAGCCCTCGCTGGTTGCCCGGCCGCGATGGCACAg                                                                                           <  5:223457/149‑1 (MQ=255)
                                            aTGAGTTCGCGCAGGAAGTGGATCTTGTGGCGGTTGCAGGACATGACGGGATACAAGCTGACAAAGTGATTGGATTATAGGCTTATTCGCGGGTTCACCCGCGAACGAGTATGAAGCCCTCGCTGGTTGCCCGGCCGCGATGGCACAGc                                                                                          <  1:45869/149‑1 (MQ=255)
                                                      gcAGGAAGTGGATCTTGTGGCGGTTGCAGGACATGACGGGATACAAGCTGACAAAGTGATTGGATTATAGGCTTATTCGCGGGTTCACCCGCGAACGAGTATGAAGCCCTCGCTGGTTGCCCGGCCGCGATGGCACAGCTTATCCTCGt                                                                                >  1:109966/1‑149 (MQ=255)
                                                             gTGGATCTTGTGGCGGTTGCAGGACATGACGGGATACAAGCTGACAAAGTGATTGGATTATAGGCTTATTCGCGGGTTCACCCGCGAACGAGTATGAAGCCCTCGCTGGTTGCCCGGCCGCGATGGCACAGCTTATCCTCGTCCCCCCt                                                                         >  7:294079/1‑149 (MQ=255)
                                                                       tgGCGGTTGCAGGACATGACGGGATACAAGCTGACAAAGTGATTGGATTATAGGCTTATTCGCGGGTTCACCCGCGAACGAGTATGAAGCCCTCGCTGGTTGCCCGGCCGCGATGGCACAGCTTATCCTCGTCCCCCCTCCATTTTTCa                                                               <  4:9626/149‑1 (MQ=255)
                                                                               gCAGGACATGACGGGATACAAGCTGACAAAGTGATTGGATTATAGGCTTATTCGCGGGTTCACCCGCGAACGAGTATGAAGCCCTCGCTGGTTGCCCGGCCGCGATGGCACAGCTTATCCTCGTCCCCCCTCCATTTTTCAGGgccgcc                                                       >  8:319025/1‑149 (MQ=255)
                                                                                cAGGACATGACGGGATACAAGCTGACAAAGTGATTGGATTATAGGCTTATTCGCGGGTTCACCCGCGAACGAGTATGAAGCCCTCGCTGGTTGCCCGGCCGCGATGGCACAGCTTATCCTCGTCCCCCCTCCATTTTTCAGGGCCGccc                                                      >  4:82459/1‑149 (MQ=255)
                                                                                 aGGACATGACGGGATACAAGCTGACAAAGTGATTGGATTATAGGCTTATTCGCGGGTTCACCCGCGAACGAGTATGAAGCCCTCGCTGGTTGCCCGGCCGCGATGGCACAGCTTATCCTCGTCCCCCCTCCATTTTTCAGGGCCGccc                                                      >  8:193765/1‑148 (MQ=255)
                                                                                    aCATGACGGGATACAAGCTGACAAAGTGATTGGATTATAGGCTTATTCGCGGGTTCACCCGCGAACGAGTATGAAGCCCTCGCTGGTTGCCCGGCCGCGATGGCACAGCTTATCCTCGTCCCCCCTCCATTTTTCAGGGCCGCCCCAAt                                                  <  3:82459/149‑1 (MQ=255)
                                                                                      aTGACGGGATACAAGCTGACAAAGTGATTGGATTATAGGCTTATTCGCGGGTTCACCCGCGAACGAGTATGAAGCCCTCGCTGGTTGCCCGGCCGCGATGGCACAGCTTATCCTCGTCCCCCCTCCATTTTTCAGGGCCGCCCCAATGa                                                <  6:240273/149‑1 (MQ=255)
                                                                                        gACGGGATACAAGCTGACAAAGTGATTGGATTATAGGCTTATTCGCGGGTTCACCCGCGAACGAGTATGAAGCCCTCGCTGGTTGACCGGCCGCGATGGCACAGCTTATCCTCGACCCCCCTCCATTTTTCAGGGCAGCACCAatcatc                                              >  8:322032/1‑149 (MQ=255)
                                                                                                  aaGCTGACAAAGTGATTGGATTATAGCCTTATTCGCGGGTTAACCCGCGAACGAGAATGAAGCCCTAGCTGATTGCCCGGACGCGATGGCACAGATTAACCACGTACCCCCACCATATTTAAGGGCCGACCCAAAGAACcaaaccccg                                     >  7:235474/1‑141 (MQ=255)
                                                                                                     cTGACAAAGTTATTTGATTATAGGCTTATTCGCGGGTTCACCCGCGAACGAGTATGAAGCCCTCGCTGGTTGCCCGGCCGCGATGGCACAGCTTATCCTCGTCCCCCCTCCATTTTTCAGGGCCGCCCCAATGATCCACAGCGCgcagc                                 <  8:168472/149‑1 (MQ=255)
                                                                                                      tGACAAAGTGATTGGATTATAGGCTTATTCGCGGGTTCACCCGCGAACGAGTATGAAGCCCTCGCTGGTTGCCCGGCCGCGATgg                                                                                                <  3:15045/85‑1 (MQ=255)
                                                                                                      tGACAAAGTGATTGGATTATAGGCTTATTCGCGGGTTCACCCGCGAACGAGTATGAAGCCCTCGCTGGTTGCCCGGCCGCGATgg                                                                                                >  4:15045/1‑85 (MQ=255)
                                                                                                      tGACAAAGTGATTGGATTATAGGCTTATTCGCGGGTTCACCCGCGAACGAGTATGAAGCCCTCGCTGGTTGCCCGGCCGCGATGGCACAGCTTATCCTCGTCCCCCCTCCATTTTTCAGGgccgcc                                                       <  8:269639/126‑1 (MQ=255)
                                                                                                      tGACAAAGTGATTGGATTATAGGCTTATTCGCGGGTTCACCCGCGAACGAGTATGAAGCCCTCGCTGGTTGCCCGGCCGCGATGGCACAGCTTATCCTCGTCCCCCCTCCATTTTTCAGGgccgcc                                                       >  7:269639/1‑126 (MQ=255)
                                                                                                       gACAAAGTGATTGGATTATAGGCTTATTCGCGGGTTCACCCGCGaa                                                                                                                                      <  8:92235/46‑1 (MQ=255)
                                                                                                       gACAAAGTGATTGGATTATAGGCTTATTCGCGGGTTCACCCGCGaa                                                                                                                                      >  7:92235/1‑46 (MQ=255)
                                                                                                                 ttGGATTATAGGCTTATTCGCGGGTTCACCCGCGAACGAGTATGAAGCCCTCGCTGGTTGCCCGGCCGCGATGGCACAGCTTATCCTCGTCCCCCCTCCATTTTTCAGGGCCGCCCCAATGATCCACAGCGCGCAGCACGCCGcctcct                     <  7:319025/149‑1 (MQ=255)
                                                                                                                 ttGGATTATAGGCTTATTCGCGGGTTCACCCGCGAACGAGTATGAAGCCCTCGCTGGTTGCCCGGCCGCGATGGCACAGCTTATCCTCGTCCCCCCTCCATTTTTCAGGGCCGCCCCAATGATCCACAGCGCGCAGCACGCCGcctcc                      <  7:322032/148‑1 (MQ=255)
                                                                                                                  tGGATTATAGGCTTATTCGCGGGTTCACCCGCGAACGAGTATGAAGCCCTCGCTGGTTGCCCGGCCGCGATGGCACAGCTTATCCTCGTCCCCCCTCCATTTTTCAGGGCCGCCCCAATGATCCACAGCGCGCAGCACGCCGCCTCCTa                    >  3:280154/1‑149 (MQ=255)
                                                                                                                    gATTATAGGCTTATTCGCGGGTTCACCCGCGAACGAGTATGAAGCCCTCGCTGGTTGCCCGGCCGCGATGGCACAGCTTATCCTCGTCCCCCCTCCATTTTTCAGGGCCGCCCCAATGATCCACAGCGCGCAGCACGCCGCCTCCTAt                   >  4:92787/1‑148 (MQ=255)
                                                                                                                     aTTATAGGCTTATTCGCGGGATCACCCGCGAACGAGTATGAAGCCCTCCCTGGTTGCCCGGCCGGGATGGCACAGCTTATCCTCGACCCCCCTCCATATTTAAGGGCCGCCCCCAAGCTCCACCGCGCGCAGCACGCGGCCTCCAATTa                 >  6:136519/1‑149 (MQ=255)
                                                                                                                         taGGCTTATTCGCGGGTTCACCCGCGAACGAGTATGAAGCCCTCGCTGGTTGCCCGGCCGCGATGGCACAGCTTATCCTCGTCCCCCCTCCATTTTTCAGGGCCGCCCCAATGATCCACAGCGCGCAGCACGCCGCCTCCTATTAcgcc             >  4:303956/1‑149 (MQ=255)
                                                                                                                            gCTTATTCGCGGGTTCACCCGCGAACGAGTTAGAAGCCCTCGCTGGTTGCCCGGCCGCGATGGCACAGCTTATCCTCGTCCCCCCTCCATTTTTCAGGGCCGCCCCAATGATCCACAGCGCGCAGCACGCCGCCTCCTATTAcgccgc           >  1:32213/1‑148 (MQ=255)
                                                                                                                              ttATTCGCGGGTTCACCCGCGAACGAGTATGAAGCCCTCGCTGGTTGCCCGGCCGCGATGGCACAGCTTATCCTCGTCCCCCCTCCATTTTTCAGGGCCGCCCCAATGATCCACAGCGCGCAGCACGCCGCCTCCTATTACGCCGCcag        >  2:369812/1‑149 (MQ=255)
                                                                                                                              tcgttCGCGGGTGAACCCGCGAACGAGTATGAAGCCCTCGCTGGTTGCCCGGCCGCGATGGCACAGCTTATCCTCGTCCCCCCTCCATTTTTCAGGGCCGCCCCAATGATCCACAGCGCGCAGCACGCCGCCTCCTATTACGCCGCcag        >  4:249130/4‑149 (MQ=255)
                                                                                                                                  tCGCGGGTTCACCCGCGAACGAGTATGAAGCACTCGCTGGTTGCCCGGCCGCGATGGCACAGCTTATCCTCGTCCCCCCTCCATTTTTCAGGGCCGCCCCAATGATCCACAGCGCGCAGCACGCCGCCTCCTATTACGCCGCCagcagc    >  2:334581/1‑149 (MQ=255)
                                                                                                                                  tCGCGGGTGAACCCGCGAACGAGTATGAAGCCCTCGCTGGTTGCCCGGCCGCGATGGCACAGCTTATCCTCGTCCCCCCTCCATTTTTCAGGGCCGCCCCAATGATCCACAGCGCGCAGCACGCCGCCTCCTATTACGCCGCCagcagc    >  8:180661/1‑149 (MQ=255)
                                                                                                                                   cgcgGGTTCACCCGCGAACGAGTATGAAGCCCTCGCTGGTTGCCCGGCCGCGATGGCACAGCTTATCCTCGTCCCCCCTCCATTTTTCAGGGCCGCCCCAATGATCCACAGCGCGCAGCACGCCGCCTCCTATTACGCCGCCagcagc    <  3:92787/148‑1 (MQ=255)
                                                                                                                                    gcgGGTTCACCCGCGAACGAGTATGAAGCCCTCGCTGGTTGCCCGGCCGCGATGGCACAGCTTATCCTCGTCCCCCCTCCATTTTTCAGGGCCGCCCCAATGATCCACAGCGCGCAGCACGCCGCCTCCTATTACGCCGCCAGCAgcgc  >  3:320542/1‑149 (MQ=255)
                                                                                                                                          |                                                                                                                                              
GGCCGCAGCAGTCGTGGCAGCCGGGCTCGCACTCGAAAGAGGGGATGAGTTCGCGCAGGAAGTGGATCTTGTGGCGGTTGCAGGACATGACGGGATACAAGCTGACAAAGTGATTGGATTATAGGCTTATTCGCGGGTTCACCCGCGAACGAGTATGAAGCCCTCGCTGGTTGCCCGGCCGCGATGGCACAGCTTATCCTCGTCCCCCCTCCATTTTTCAGGGCCGCCCCAATGATCCACAGCGCGCAGCACGCCGCCTCCTATTACGCCGCCAGCAGCGC  >  NC_002947/6021562‑6021842

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑