Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,046,171 C→G 8.2% E443Q (GAG→CAG)  puuA‑II ← glutamate‑putrescine ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,046,1710CG 8.2% 148.4 / 5.0 49E443Q (GAG→CAG) puuA‑IIglutamate‑putrescine ligase
Reads supporting (aligned to +/- strand):  ref base C (24/21);  new base G (2/2);  total (26/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.93e-01

GTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGGTACCAGTTGTACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATGTAGTCCGGGCTGATGTATTGGTTGAGGACTTCGCTGTCATCCAGCGCGCGCAGGGCATCGCGCAGGTTGTTCGGCAGGCTTTGCTCCAGCTGCTCGTAG  >  NC_002947/6046027‑6046310
                                                                                                                                                |                                                                                                                                            
gTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGGTACCAGTTGTACTCAAGATCGGAAATCGACACTTCGAACTCGGc                                                                                                                                           <  1:368338/148‑1 (MQ=255)
        ccGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGGTACCAGTTGTACTCAAGATCGGAAATCGACACTTCGAACTGGCCGAGTTCgaa                                                                                                                                  <  2:186024/149‑3 (MQ=255)
          gtgtGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGGTACCAGTTGTACTCAAGATCGGAAATCGACACTTCGAACTGGCCGAGTTCgaagt                                                                                                                                <  3:97114/149‑5 (MQ=255)
             tgCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGGTACCAGTTGTACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTcc                                                                                                                              <  6:166905/148‑1 (MQ=255)
                         ttcCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGGTACCAGTTGTACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACg                                                                                                                 >  1:14276/1‑148 (MQ=255)
                            ccTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGGTACCAGTTGTACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAg                                                                                                              <  6:207595/148‑1 (MQ=255)
                                    ctgACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGGTACCAGTTGTACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGAt                                                                                                      <  3:282868/148‑1 (MQ=255)
                                    ctgACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGGTACCAGTTGTACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTCCGCTTTCCTTGCAGGCC‑ACGAAGATATCGa                                                                                                       <  2:58945/147‑1 (MQ=255)
                                       aCGGCAGGCGCTGACACCGATCAAAGGGACCGAATTTGCGCTCATGGGCTTACACCGTGTGCAGGTACCAGTTGTACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATGTa                                                                                                   <  6:213085/148‑1 (MQ=255)
                                            aGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGGTACCAGTTGTACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGAt                                                                                                      <  4:303205/140‑1 (MQ=255)
                                            aGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGGTACCAGTTGTACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGAt                                                                                                      >  3:303205/1‑140 (MQ=255)
                                             ggCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGGTACCAGTTGTACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATGTAGTCCggg                                                                                            <  2:14276/149‑1 (MQ=255)
                                                 cTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGGTACCAGTTCTACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATGTAGTCCGGGCTGa                                                                                        <  6:244272/149‑1 (MQ=255)
                                                    acacCGATCAAAGGGACCGCATTTGCGTTCATGGGCTTACACCGTGTGCAGGTACCAGTTGTACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATGTAGTCCGGGCTGAt                                                                                       <  1:171695/147‑1 (MQ=255)
                                                      acCGATCAAAGGGACCGCATTTGCTCTCATGGGCTTACACCGTGTGCAGGTACCAGTTGTACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATGTAGTCCGGGCTGATGTAt                                                                                   >  3:223362/1‑149 (MQ=255)
                                                        cGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGGTACCAGTTGTACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATGTAGTCCGGGCTGAt                                                                                       <  2:245035/143‑1 (MQ=255)
                                                        cGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGGTACCAGTTGTACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATGTAGTCCGGGCTGAt                                                                                       >  1:245035/1‑143 (MQ=255)
                                                        cGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGGTACCAGTTGTACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATGTAGTCCGGGCTGATGTATTg                                                                                 >  5:17951/1‑149 (MQ=255)
                                                         gATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGGTACCAGTTGTACTCAAGATCGCAAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATGTAGTCCGGGCTGATGTAt                                                                                   >  5:261427/1‑146 (MQ=255)
                                                             aaaGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGGTACCAGTTGTACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATGTAGTCCGGGCTGATGTATTGGTTGa                                                                            <  7:300773/149‑1 (MQ=255)
                                                              aaGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGGTACCAGTTGTACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATg                                                                                                     >  6:123459/1‑123 (MQ=255)
                                                                 ggACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGGTACCAGTTGTACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTGCTTGGAGGCC‑ACGAAGATATCGATGTAGTCCGGGCTGATGTATTGGTTGAGGAc                                                                        >  8:235151/1‑149 (MQ=255)
                                                                 ggACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGGTACCAGTTGTACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATGTAGTCCGGGCTGATGTATTGGTTGAGGAc                                                                        <  2:364278/149‑1 (MQ=255)
                                                                      gCATTTGCGCTCATGGGCTTACACCGTGTGCAGGTACCAGTTGTACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATGTAGTCCGGGCTGATGTATTGGTTGAGGACTTCGc                                                                   <  6:17951/149‑1 (MQ=255)
                                                                                   tGGGCTTACACCGTGTGCAGGTACCAGTTGTACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATGTAGTCCGGGCTGATGTATTGGTTGAGGACTTCGCTGTCATCCAgcgc                                                      >  6:337044/1‑149 (MQ=255)
                                                                                            acCGTGTGCAGGTACCAGTTGTACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATGTAGTCCGGGCTGATGTATTGGTTGAGGACTTCGCTGTCATCCAGCGCGCGCAggg                                              >  7:111023/1‑148 (MQ=255)
                                                                                               gtgtGCAGGTACCAGTTGTACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATGTAGTCCGGGCTGATGTATTGGTTGAGGACTTCGCTGTCATCCAGCGCGCGCAGGGCATc                                          <  4:223362/149‑1 (MQ=255)
                                                                                                     aGGTACCAGTTGTACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATGTAGTCCGGGCTGATGTATTGGTTGAGGACTTCGCTGTCATCCAGCGCGCGCAGGGCATCGCGCAg                                    >  4:252639/1‑149 (MQ=255)
                                                                                                       gTACCAGTTGTACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATGTAGTCCGGGCTGATGTATTGGTTGAGGACTTCGCTGTCATCCa                                                          <  5:12094/125‑1 (MQ=255)
                                                                                                       gTACCAGTTGTACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATGTAGTCCGGGCTGATGTATTGGTTGAGGACTTCGCTGTCATCCAGCGCGCGCAGGGCATCGCGCAGgt                                  >  8:3878/1‑149 (MQ=255)
                                                                                                       gTACCAGTTGTACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATGTAGTACGGGCTGATGTATTGGTTGAGGACTTCGCTGTCATCCa                                                          >  6:12094/1‑125 (MQ=255)
                                                                                                         aCCAGTTGTACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATGTAGTCCGGGCTGATGTATTGGTTGAGGACTTCGCTGTCATCCAGCGCGCGCAGGGCATCGCGCAGgttg                                >  1:229726/1‑149 (MQ=255)
                                                                                                            aGTTGTACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATGTAGTCCGGGCTGATGT‑TTGGTTGAGGACTTCGCTGTCATCCAGCGCGCGCAGGGCATCGCGCAGGTTGTTCg                            >  7:65845/1‑149 (MQ=255)
                                                                                                             gTTGTACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATGTAGTCCGGGCTGATGTATTGGTTGAGGACTTCGCTGTCATCCAGCGCGCGCAGGGCATCGCGCAGGTTGTTCg                            >  5:251515/1‑149 (MQ=255)
                                                                                                              ttGTACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCCTACGAAGATATCGATGTAGTCCGGGCTGATGTATTgg                                                                                <  1:246898/97‑1 (MQ=255)
                                                                                                              ttGTACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATGTAGTCCGGGCTGATGTATTgg                                                                                >  2:246898/1‑96 (MQ=255)
                                                                                                              ttGTACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATGTAGTCCGGGCTGATGTATTGGTTGAGGACTTCGCTGTCATCCAGCGCGCGCAGGGCATCGCGCAGGTTGTTCgg                           >  1:26132/1‑149 (MQ=255)
                                                                                                                gTACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATGTAGTCCGGGCTGATGTATTGGTTGa                                                                            <  1:46365/98‑1 (MQ=255)
                                                                                                                gTACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATGTAGTCCGGGCTGATGTATTGGTTGa                                                                            >  2:46365/1‑98 (MQ=255)
                                                                                                                 tACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCTCTTTCCTTGCAGGCC‑ACGAAGATATTTATGTAGTCCGGGCTGATGTATTGGTTGAGGACTTCGCTGTCATCCAGCGCGCGCTGAGCATCGCGCAGGTTGTTCGGCa                         <  7:235151/148‑1 (MQ=37)
                                                                                                                   cTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATGTAGTCCGGGCAGATGTATTGGTTGAGGAGTTCGCTGTCATACAGCGCGCGCAGGGCCTGGGGCAGGTTGTTCGGCAGGc                      >  4:30615/1‑149 (MQ=255)
                                                                                                                    tCAAGATCGGTAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATGTAGTCCGGGCTGATGTATTGGTTGAGGACTTCGCTGTCATCCAGCGCGCGCAGGGCATCGCGCAGGTTGTTCGGCAGGCt                     >  6:346158/1‑149 (MQ=255)
                                                                                                                    tCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATGTAGTCCGGGCTGATGTATTGGTTGAGGACTTCGCTGTCATCCAGCGCGCGCAGGGCATCGCGCAGGTTGTTCGACAGGCt                     >  6:194289/1‑149 (MQ=255)
                                                                                                                               aaTCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATGTAGTCCGGGCTGATGTATTGGTTGAGGACTTCGCTGTCATCCAGCGCGCGCAGGGCATCGCGCAGGTTGTTCGGCAGGCTTTGCTCCAgct          >  7:125330/1‑149 (MQ=255)
                                                                                                                                    acacTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATGTAGTCCGGGCTGATGTATTGGTTGAGGACTTCGCTGTCATCCAGCGCGCGCAGGGCATCGCGCAGGTTGTTCGGCAGGCTTTGCTCCAGCTGCTCg     <  5:310201/149‑1 (MQ=255)
                                                                                                                                      acTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATGTAGTCCGGGCTGATGTATTGGTTGAGGACTTCGCTGTCATCCAGCGCGCGCAGGGCATCGCGCAGGTTGTTCGGCAGGCTTTGCTCCAGCTGCTCGTa   <  2:229726/149‑1 (MQ=255)
                                                                                                                                       aagcGAACTGGCCGAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGTTGTAGTCCGGGCTGAGGTATTGGTTGAGGACTTCGCGGTCATCCAGCGCGCGCAGGGCATCGCGGAGGTGGTTCGGCGGGCTTTGGTCGAGCTGCTCggtg  >  8:58308/4‑146 (MQ=255)
                                                                                                                                       aagcGAACTGGCCGAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATGTAGTCCGGGCTGATGTATTGGTTGAGGACTTCGCTGTCATCCAGCGCGCGCAGGGCATCGCGCAGGTTGTTCGGCAGGCTTTGCTCCAGCTGCTCGTAg  >  4:175573/4‑149 (MQ=255)
                                                                                                                                        ttCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATGTAGTCCGGGCTGATGTATTGGTTGAGGACTTCGCTGTCATCCAGCGCGCGCAGGGCATCGCGCAGGTTGTTCGGCAGGCTTTGCTCCAGCTGCTCGTAg  >  1:130883/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                            
GTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGGTACCAGTTGTACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATGTAGTCCGGGCTGATGTATTGGTTGAGGACTTCGCTGTCATCCAGCGCGCGCAGGGCATCGCGCAGGTTGTTCGGCAGGCTTTGCTCCAGCTGCTCGTAG  >  NC_002947/6046027‑6046310

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑