Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,046,175 C→G 8.0% L441L (CTG→CTC)  puuA‑II ← glutamate‑putrescine ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,046,1750CG 8.0% 147.6 / 2.8 49L441L (CTG→CTC) puuA‑IIglutamate‑putrescine ligase
Reads supporting (aligned to +/- strand):  ref base C (24/21);  new base G (2/2);  total (26/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01

CCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGGTACCAGTTGTACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATGTAGTCCGGGCTGATGTATTGGTTGAGGACTTCGCTGTCATCCAGCGCGCGCAGGGCATCGCGCAGGTTGTTCGGCAGGCTTTGCTCCAGCTGCTCGTAGGAGTTGCCTTC  >  NC_002947/6046035‑6046321
                                                                                                                                            |                                                                                                                                                   
ccGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGGTACCAGTTGTACTCAAGATCGGAAATCGACACTTCGAACTGGCCGAGTTCgaa                                                                                                                                             <  2:186024/149‑3 (MQ=255)
  gtgtGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGGTACCAGTTGTACTCAAGATCGGAAATCGACACTTCGAACTGGCCGAGTTCgaagt                                                                                                                                           <  3:97114/149‑5 (MQ=255)
     tgCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGGTACCAGTTGTACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTcc                                                                                                                                         <  6:166905/148‑1 (MQ=255)
                 ttcCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGGTACCAGTTGTACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACg                                                                                                                            >  1:14276/1‑148 (MQ=255)
                    ccTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGGTACCAGTTGTACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAg                                                                                                                         <  6:207595/148‑1 (MQ=255)
                            ctgACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGGTACCAGTTGTACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGAt                                                                                                                 <  3:282868/148‑1 (MQ=255)
                            ctgACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGGTACCAGTTGTACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTCCGCTTTCCTTGCAGGCC‑ACGAAGATATCGa                                                                                                                  <  2:58945/147‑1 (MQ=255)
                               aCGGCAGGCGCTGACACCGATCAAAGGGACCGAATTTGCGCTCATGGGCTTACACCGTGTGCAGGTACCAGTTGTACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATGTa                                                                                                              <  6:213085/148‑1 (MQ=255)
                                    aGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGGTACCAGTTGTACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGAt                                                                                                                 <  4:303205/140‑1 (MQ=255)
                                    aGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGGTACCAGTTGTACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGAt                                                                                                                 >  3:303205/1‑140 (MQ=255)
                                     ggCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGGTACCAGTTGTACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATGTAGTCCggg                                                                                                       <  2:14276/149‑1 (MQ=255)
                                         cTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGGTACCAGTTCTACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATGTAGTCCGGGCTGa                                                                                                   <  6:244272/149‑1 (MQ=255)
                                            acacCGATCAAAGGGACCGCATTTGCGTTCATGGGCTTACACCGTGTGCAGGTACCAGTTGTACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATGTAGTCCGGGCTGAt                                                                                                  <  1:171695/147‑1 (MQ=255)
                                              acCGATCAAAGGGACCGCATTTGCTCTCATGGGCTTACACCGTGTGCAGGTACCAGTTGTACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATGTAGTCCGGGCTGATGTAt                                                                                              >  3:223362/1‑149 (MQ=255)
                                                cGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGGTACCAGTTGTACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATGTAGTCCGGGCTGAt                                                                                                  <  2:245035/143‑1 (MQ=255)
                                                cGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGGTACCAGTTGTACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATGTAGTCCGGGCTGAt                                                                                                  >  1:245035/1‑143 (MQ=255)
                                                cGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGGTACCAGTTGTACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATGTAGTCCGGGCTGATGTATTg                                                                                            >  5:17951/1‑149 (MQ=255)
                                                 gATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGGTACCAGTTGTACTCAAGATCGCAAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATGTAGTCCGGGCTGATGTAt                                                                                              >  5:261427/1‑146 (MQ=255)
                                                     aaaGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGGTACCAGTTGTACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATGTAGTCCGGGCTGATGTATTGGTTGa                                                                                       <  7:300773/149‑1 (MQ=255)
                                                      aaGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGGTACCAGTTGTACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATg                                                                                                                >  6:123459/1‑123 (MQ=255)
                                                         ggACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGGTACCAGTTGTACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTGCTTGGAGGCC‑ACGAAGATATCGATGTAGTCCGGGCTGATGTATTGGTTGAGGAc                                                                                   >  8:235151/1‑149 (MQ=255)
                                                         ggACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGGTACCAGTTGTACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATGTAGTCCGGGCTGATGTATTGGTTGAGGAc                                                                                   <  2:364278/149‑1 (MQ=255)
                                                              gCATTTGCGCTCATGGGCTTACACCGTGTGCAGGTACCAGTTGTACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATGTAGTCCGGGCTGATGTATTGGTTGAGGACTTCGc                                                                              <  6:17951/149‑1 (MQ=255)
                                                                           tGGGCTTACACCGTGTGCAGGTACCAGTTGTACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATGTAGTCCGGGCTGATGTATTGGTTGAGGACTTCGCTGTCATCCAgcgc                                                                 >  6:337044/1‑149 (MQ=255)
                                                                                    acCGTGTGCAGGTACCAGTTGTACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATGTAGTCCGGGCTGATGTATTGGTTGAGGACTTCGCTGTCATCCAGCGCGCGCAggg                                                         >  7:111023/1‑148 (MQ=255)
                                                                                       gtgtGCAGGTACCAGTTGTACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATGTAGTCCGGGCTGATGTATTGGTTGAGGACTTCGCTGTCATCCAGCGCGCGCAGGGCATc                                                     <  4:223362/149‑1 (MQ=255)
                                                                                             aGGTACCAGTTGTACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATGTAGTCCGGGCTGATGTATTGGTTGAGGACTTCGCTGTCATCCAGCGCGCGCAGGGCATCGCGCAg                                               >  4:252639/1‑149 (MQ=255)
                                                                                               gTACCAGTTGTACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATGTAGTCCGGGCTGATGTATTGGTTGAGGACTTCGCTGTCATCCa                                                                     <  5:12094/125‑1 (MQ=255)
                                                                                               gTACCAGTTGTACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATGTAGTCCGGGCTGATGTATTGGTTGAGGACTTCGCTGTCATCCAGCGCGCGCAGGGCATCGCGCAGgt                                             >  8:3878/1‑149 (MQ=255)
                                                                                               gTACCAGTTGTACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATGTAGTACGGGCTGATGTATTGGTTGAGGACTTCGCTGTCATCCa                                                                     >  6:12094/1‑125 (MQ=255)
                                                                                                 aCCAGTTGTACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATGTAGTCCGGGCTGATGTATTGGTTGAGGACTTCGCTGTCATCCAGCGCGCGCAGGGCATCGCGCAGgttg                                           >  1:229726/1‑149 (MQ=255)
                                                                                                    aGTTGTACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATGTAGTCCGGGCTGATGT‑TTGGTTGAGGACTTCGCTGTCATCCAGCGCGCGCAGGGCATCGCGCAGGTTGTTCg                                       >  7:65845/1‑149 (MQ=255)
                                                                                                     gTTGTACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATGTAGTCCGGGCTGATGTATTGGTTGAGGACTTCGCTGTCATCCAGCGCGCGCAGGGCATCGCGCAGGTTGTTCg                                       >  5:251515/1‑149 (MQ=255)
                                                                                                      ttGTACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCCTACGAAGATATCGATGTAGTCCGGGCTGATGTATTgg                                                                                           <  1:246898/97‑1 (MQ=255)
                                                                                                      ttGTACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATGTAGTCCGGGCTGATGTATTgg                                                                                           >  2:246898/1‑96 (MQ=255)
                                                                                                      ttGTACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATGTAGTCCGGGCTGATGTATTGGTTGAGGACTTCGCTGTCATCCAGCGCGCGCAGGGCATCGCGCAGGTTGTTCgg                                      >  1:26132/1‑149 (MQ=255)
                                                                                                        gTACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATGTAGTCCGGGCTGATGTATTGGTTGa                                                                                       <  1:46365/98‑1 (MQ=255)
                                                                                                        gTACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATGTAGTCCGGGCTGATGTATTGGTTGa                                                                                       >  2:46365/1‑98 (MQ=255)
                                                                                                         tACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCTCTTTCCTTGCAGGCC‑ACGAAGATATTTATGTAGTCCGGGCTGATGTATTGGTTGAGGACTTCGCTGTCATCCAGCGCGCGCTGAGCATCGCGCAGGTTGTTCGGCa                                    <  7:235151/148‑1 (MQ=37)
                                                                                                           cTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATGTAGTCCGGGCAGATGTATTGGTTGAGGAGTTCGCTGTCATACAGCGCGCGCAGGGCCTGGGGCAGGTTGTTCGGCAGGc                                 >  4:30615/1‑149 (MQ=255)
                                                                                                            tCAAGATCGGTAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATGTAGTCCGGGCTGATGTATTGGTTGAGGACTTCGCTGTCATCCAGCGCGCGCAGGGCATCGCGCAGGTTGTTCGGCAGGCt                                >  6:346158/1‑149 (MQ=255)
                                                                                                            tCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATGTAGTCCGGGCTGATGTATTGGTTGAGGACTTCGCTGTCATCCAGCGCGCGCAGGGCATCGCGCAGGTTGTTCGACAGGCt                                >  6:194289/1‑149 (MQ=255)
                                                                                                                       aaTCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATGTAGTCCGGGCTGATGTATTGGTTGAGGACTTCGCTGTCATCCAGCGCGCGCAGGGCATCGCGCAGGTTGTTCGGCAGGCTTTGCTCCAgct                     >  7:125330/1‑149 (MQ=255)
                                                                                                                            acacTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATGTAGTCCGGGCTGATGTATTGGTTGAGGACTTCGCTGTCATCCAGCGCGCGCAGGGCATCGCGCAGGTTGTTCGGCAGGCTTTGCTCCAGCTGCTCg                <  5:310201/149‑1 (MQ=255)
                                                                                                                              acTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATGTAGTCCGGGCTGATGTATTGGTTGAGGACTTCGCTGTCATCCAGCGCGCGCAGGGCATCGCGCAGGTTGTTCGGCAGGCTTTGCTCCAGCTGCTCGTa              <  2:229726/149‑1 (MQ=255)
                                                                                                                               aagcGAACTGGCCGAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGTTGTAGTCCGGGCTGAGGTATTGGTTGAGGACTTCGCGGTCATCCAGCGCGCGCAGGGCATCGCGGAGGTGGTTCGGCGGGCTTTGGTCGAGCTGCTCggtg             >  8:58308/4‑146 (MQ=255)
                                                                                                                               aagcGAACTGGCCGAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATGTAGTCCGGGCTGATGTATTGGTTGAGGACTTCGCTGTCATCCAGCGCGCGCAGGGCATCGCGCAGGTTGTTCGGCAGGCTTTGCTCCAGCTGCTCGTAg             >  4:175573/4‑149 (MQ=255)
                                                                                                                                ttCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATGTAGTCCGGGCTGATGTATTGGTTGAGGACTTCGCTGTCATCCAGCGCGCGCAGGGCATCGCGCAGGTTGTTCGGCAGGCTTTGCTCCAGCTGCTCGTAg             >  1:130883/1‑148 (MQ=255)
                                                                                                                                         ggCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATGTAGTCCGGGCTGATGTATTGGTTGAGGACTTCGCTGTCATCCAGCGCGCGCAGGGCATCGCGCAGGTTGTTCGGCAGGCTTTGCTCCAGCTGCTCGTAGGAGTTGCCtt   <  5:337044/149‑1 (MQ=255)
                                                                                                                                           ccAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATGTAGTCCGGGCTGATGTATTGGTTGAGGACTTCGCTGTCATCCAGCGCGCGCAGGGCATCGCGCAGGTTGTTCGGCAGGCTTTGCTCCAGCTGCTCGTAGGAGATGCCTTc  >  4:27349/1‑148 (MQ=255)
                                                                                                                                            |                                                                                                                                                   
CCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGGTACCAGTTGTACTCAAGATCGGAAATCGACACTTCGAACTCGGCCAGTTCGCTTTCCTTGCAGGCC‑ACGAAGATATCGATGTAGTCCGGGCTGATGTATTGGTTGAGGACTTCGCTGTCATCCAGCGCGCGCAGGGCATCGCGCAGGTTGTTCGGCAGGCTTTGCTCCAGCTGCTCGTAGGAGTTGCCTTC  >  NC_002947/6046035‑6046321

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑