Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,112,863 C→T 7.2% intergenic (‑119/‑8) PP_5363 ← / → clsA hypothetical protein/cardiolipin synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,112,8630CT 7.2% 161.0 / 2.2 55intergenic (‑119/‑8)PP_5363/clsAhypothetical protein/cardiolipin synthase
Reads supporting (aligned to +/- strand):  ref base C (25/26);  new base T (2/2);  total (27/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.33e-01

CAACGCCAACAGTGGCAGACGAATCTTCATGGACGAACGCTCCTGGAAAAAAGCCGGTCAAACGGGCCGGACTGAGCCTTAGACGCCAAATCCGCCTGCGGGTTGCAGGCGGACCGGGAACGTGTTCTGTTACCTCAAGTGTACTCGGCAAGCAGGATGCATATGGATTACCACAGCCCCTACTTCTTTGGTTACCTACTCGGCCTGATCCATCTGCTGGGTATTGTCGCCGCACTGCATGCGCTGTTCACCGTGCGGACAGCCCAGGGCGCCATTGCTTGGGCGATGCCCTTGC  >  NC_002947/6112715‑6113009
                                                                                                                                                    |                                                                                                                                                  
caacgccaacAGTGGCAGACGAATCTTCATGGACGAACGCTCCTGGAAAAAAGCCGGTCAAACGGGCCGGACTGAGCCTTAGACGCCAAATCCGCCTGCGGGTTGCAGGCGGACCGGGAACGTGTTCTGTTACCTCAAGTGTACTCGGc                                                                                                                                                    <  4:124745/149‑1 (MQ=255)
     ccaacAGTGGCAGACGAATCTTCATGGACGAACGCTCCTGGAAAAAAGCCGGTCAAACGGGCCGGACTGAGCCTTAGACGCCAAATCCGCCTGCGGGTTGCAGGCGGACCGGGAACGTGTTCTGTTACCTCAAGTGTACTCGGCAAGCa                                                                                                                                               <  1:331631/149‑1 (MQ=255)
                aGACGAATCTTCATGGACGAACGCTCCTGGAAAAAAGCCGGTCAAACGGGCCGGACTGAGCCTTAGACGCCAAATCCGCCTGCGGGTTGCAGGCGGACCGGGAACGTGTTCTGTTACCTCAAGTGTACTCGGCAAGCAGGATGCATATg                                                                                                                                    <  3:24723/149‑1 (MQ=255)
                  aCGAATCTTCATGGACGAACGCTCCTGGAAAAAAGCCGGTCAAACGGGCCGGACTGAGCCTTAGACGCCAAATCCGCCTGCGGGTTGCAGGCGGACCGGGAACGTGTTCTGTTACCTCAAGTGTACTCGGCAAGCAGGATGCATATGGa                                                                                                                                  >  7:205441/1‑149 (MQ=255)
                      aTCTTCATGGACGAACGCTCCTGGAAAAAAGCCGGTCAAACGGGCCGGACTGAGCCTTAGACGCCAAATCCGCCTGCGGGTTGCAGGCGGACCGGGAACGTGTTCTGTTACCTCAAGTGTACTCGGCAAGCAGGATGCATATGGATTAc                                                                                                                              <  3:352018/149‑1 (MQ=255)
                          tCATGGACGAACGCTCCTGGAAAAAAGCCGGTCAAACGGGCCGGACTGAGCCTTAGACGCCAAATCCGCCTGCGGGTTGCAGGCGGACCGGGAACGTGTTCTGTTACCTCAAGTGTACTCGGCAAGCAGGATGCATATGGATTACcaca                                                                                                                          >  2:184909/1‑149 (MQ=255)
                             tGGACGAACGCTCCTGGAAAAAAGCCGGTCAAACGGGCCGGACTGAGCCTTAGACGCCAAATCCGCCTGCGGGTTGCAGGCGGACCGGGAACGTGTTCTGTTACCTCAAGTGTACTCGGCAAGCAGGATGCATATGGATTACCACAGcc                                                                                                                       <  2:2821/149‑1 (MQ=255)
                              ggACGAACGCTCCTGGAAAAAAGCCGGTCAAACGGGCCGGACTGAGCCTTAGACGCCAAATCCGCCTGCGGGTTGCAGGCGGACCGGGAACGTGTTCTGTTACCTCAAGTGTACTCGGCAAGCAGGATGCATATGGATTACCACAGccc                                                                                                                      >  6:204322/1‑149 (MQ=255)
                                        tCCTGGAAAAAAGCCGGTCAAACGGGCCGGACTGAGCCTTAGAAGCCAAATCCGCCTGCGGGTTGCAGGCGGACCGGGAACGTGTTCTGTTACCTCAAGTGTACTCGGCAAGCAGGATGCATATGGATTACCACAGCCCCTACTACttt                                                                                                            >  8:244594/1‑149 (MQ=255)
                                          cTGGAAAAAAGCCGGTCAAACGGGCCGGACTGAGCCTTAGACGCCAAATCCGCCTGCGGGTTGCAGGCGGACCGGGAACGTGTTCTGTTACCTCAAGTGTACTCGGCAAGCAGGATGCATATGGATTACCACAGCCCCTACTTCTTTgg                                                                                                          >  4:35394/1‑149 (MQ=255)
                                          cTGGAAAAAAGCCGGTCAAACGGGCCGGACTGAGCCTTAGACGCCAAATCCGCCTGCGGGTTGCAGGCGGACCGGGAACGTGTTCTGTTACCTCAAGTGTACTCGGCAAGCAGGATGCATATGGATTACCACAGCCCCTACTTCTTTg                                                                                                           <  4:174774/148‑1 (MQ=255)
                                             gAAAAAAGCCGGTCAAACGGGCCGGACTGAGCCTTAGACGCCAAATCCGCCTGCGGGTTGCAGGCGGACCGGGAACGTGTTCTGTTACCTCAAGTGTACTCGGCAAGCAGGATGCATATGGATTACCACAGc                                                                                                                        >  6:271196/1‑132 (MQ=255)
                                             gAAAAAAGCCGGTCAAACGGGCCGGACTGAGCCTTAGACGCCAAATCCGCCTGCGGGTTGCAGGCGGACCGGGAACGTGTTCTGTTACCTCAAGTGTACTCGGCAAGCAGGATGCATATGGATTACCACAGc                                                                                                                        <  5:271196/132‑1 (MQ=255)
                                              aaaaaaGCCGGTCAAACGGGCCGGACTGAGCCTTAGACGCCAAATCCGCCTGCGGGTTGCAGGCGGACCGGGAACGTGTTCTGTTACCTCAAGTGTACTCGGCAAGCAGGATGCATATGGATTACCACAGCCCCTACTTCTTTGGTTAc                                                                                                      <  8:205441/149‑1 (MQ=255)
                                              aaaaaaCCCGGTCAAACGGGCCGGACTGAGCCTTAGACGCCAAATCCGCCTGCGGGTTGCAGGCGGACCGGGAACGTGTTCTGTTACCTCAAGTGTACTCGGCAAGCAGGATGCATATGGATTACCACAGCCCCTACTTCTTTGGTTAc                                                                                                      <  6:299839/149‑1 (MQ=255)
                                                aaaaGCCGGTCAAACGGGCCGGACTGAGCCTTAGACGCCAAATCCGCCTGCGGGTTGCAGGCGGACCGGGAACGTGTTCTGTTACCTCAAGTGTACTCGTCAAGCAGGATGCATATGGATTACCACAGCCCCTACTTCTTTGGTTAcc                                                                                                     <  1:314820/148‑1 (MQ=255)
                                                  aaGCCGGTCAAACGGGCCGGACTGAGCCTTAGACGCCAAATCCGCCTGCGGGTTGCAGGCGGACCGGGAACGTGTTCTGTTACCTCAAGTGTACTCGGCAAGCAGGATGCATATGGATTACCACAGCCCCTACTTCTTTGGTTACCTAc                                                                                                  >  8:348442/1‑149 (MQ=255)
                                                                 gCCGGACTGAGCCTTAGACGCCAAATCCGCCTGCGGGTTGCAGGCGGACCGGGAACGTGTTCTGTTACCTCAAGTGTACTCGGCAAGCAGGATGCATATGGATTACCACAGCCCCTACTTCTTTGGTTACCTACTCGGcc                                                                                            <  5:237536/140‑1 (MQ=255)
                                                                 gCCGGACTGAGCCTTAGACGCCAAATCCGCCTGCGGGTTGCAGGCGGACCGGGAACGTGTTCTGTTACCTCAAGTGTACTCGGCAAGCAGGATGCATATGGATTACCACAGCCCCTACTTCTTTGGTTACCTACTCGGcc                                                                                            >  6:237536/1‑140 (MQ=255)
                                                                       cTGAGCCTTAGACGCCAAATCCGCCTGCGGGTTGCAGGCGGACCGGGAACGTGTTCTGTTACCTCAAGTGTACTCGGCAAGCAGGATGCATATGGATTACCACAGCCCCTACTTCTTTGGTTACCTACTCGGCCTGATCCATCTGCTgg                                                                             <  8:197862/149‑1 (MQ=255)
                                                                       cTGAGCCTTAGACGCCAAATCCGCCTGCGGGTTGCAGGCGGACCGGGAACGTGTTCTGTTACCTCAAGTGTACTCGGCAAGCAGGATGCATATGGATTACCACAGCCCCTACTTCTTTGGTTACCTACTCGGCCTGATCCATCTGCTgg                                                                             <  5:204322/149‑1 (MQ=255)
                                                                            ccTTAGACGCCAAATCCGCCTGCGGGTTGCAGGCGGACCGGGAACGTGTTCTGTTACCTCAAGTGTACTCGGTAAGCAGGATGCATATGGATTACCACAGCCCCTACTTCTTTgg                                                                                                          >  3:321272/1‑115 (MQ=255)
                                                                            ccTTAGACGCCAAATCCGCCTGCGGGTTGCAGGCGGACCGGGAACGTGTTCTGTTACCTCAAGTGTACTCGGTAAGCAGGATGCATATGGATTACCACAGCCCCTACTTCTTTgg                                                                                                          <  4:321272/115‑1 (MQ=255)
                                                                            ccTTAGACGCCAAATCCGCCTGCGGGTTGCAGGCGGACCGGGAACGTGTTCTGTTACCTCAAGTGTACTCGGCAAGCAGGATGCATATGGATTACCACAGCCCCTACTTCTTTGGTTACCTACTCGGc                                                                                             <  1:254072/128‑1 (MQ=255)
                                                                            ccTTAGACGCCAAATCCGCCTGCGGGTTGCAGGCGGACCGGGAACGTGTTCTGTTACCTCAAGTGTACTCGGCAAGCAGGATGCATATGGATTACCACAGCCCCTACTTCTTTGGTTACCTACTCGGc                                                                                             >  2:254072/1‑128 (MQ=255)
                                                                              ttAGACGCCAAATCCGCCTGCGGGTTGCAGGCGGACCGGGAACGTGTTCTGTTACCTCAAGTGTACTCGGCAAGCAGGATGCATATGGATTACCACAGCCCCTACTTCTTTGGTTACCTACTCGGCCTGATCCATCTGCTGGGTATTg                                                                       >  4:13319/1‑148 (MQ=255)
                                                                                    gCCAAATCCGCCTGCGGGTTGCAGGCGGACCGGGAACGTGTTCTGTTACCTCAAGTGTACTCGGCaa                                                                                                                                                  <  7:133038/67‑1 (MQ=255)
                                                                                    gCCAAATCCGCCTGCGGGTTGCAGGCGGACCGGGAACGTGTTCTGTTACCTCAAGTGTACTCGGCaa                                                                                                                                                  >  8:133038/1‑67 (MQ=255)
                                                                                    gCCAAATCCGCCTGCGGGTTGCAGGCGGACCGGGAACGTGTTCTGTTACCTCAAGTGTACTCGGCAAGCAGGATGCATATGGATTACCACAGCCCCTACTTCTTTGGTTACCTACTCGGCCTGATCCATCTGCTGGTTATTGTcgacg                                                                 >  4:111209/1‑148 (MQ=255)
                                                                                    gCCAAATCCGCCTGCGGGTTGCAGGCGGACCGGGAACGTGTTCTGTTACCTCAAGTGTACTCGGCAAGCAGGATGCATATGGATTACCACAGCCCCTACTTCTTTGGTTACCTACTCGGCCTGATCCATCTGCTGGGTATTGTcgccg                                                                 >  2:14672/1‑148 (MQ=255)
                                                                                      cAAATCCGCCTGCGGGTTGCAGGCGGACCGGGAACGTGTTCTGTTACCTCAAGTGTACTCGGCAAGCAGCATGCATATGGATTACCACAGCCCCTACTTCTTTGGTTACCTACTCGGCCTGATCCATCTGCTGGGTATTGTCGCCGCAc                                                              >  7:114085/1‑149 (MQ=255)
                                                                                         aTCCGCCTGCGGGTTGCAGGCGGACCGGGAACGTGTTCTGTTACCTCAAGTGTACTCGGCAAGCAGGATGCATATGGATTACCACAGCCCCTACTTCTTTGGTTACCTACTCGGCCTGATCCATCTGCTGGGTATTGTCGCCGCACTGc                                                           <  1:14672/149‑1 (MQ=255)
                                                                                         aTCCGCCTGCGGGTTGCAGGCGGACCGGGAACGTGTTCTGTTACCTCAAGTGTACTCGGCAAGCAGGATGCATATGGATTACCACAGCCCCTACTTCTTTGGTTACCTACTCGGCCTGATCCATCTGCTGGGTATTGTCGCCGCACTGc                                                           <  3:35394/149‑1 (MQ=255)
                                                                                          tCCGCCTGCGGGTTGCAGGCGGACCGGGAACGTGTTCTGTTACCTCAAGTGTACTCGGCAAGCAGGATGCATATGGATTACCACATCCCCTACTTCTTTGGTTACCTACTCGGCCTGATCCATCTGCTGGGTATTGTCGCCGCACTGCa                                                          >  5:351822/1‑149 (MQ=255)
                                                                                                  cGGGTTGCAGGCGGACCGGGAACGTGTTCTGTTACCTCAAGTGTACTCGGCAAGCAGGATGCATATGGATTACCACAGCCCCTACTTCTTTGGTTACCTACTCGGCCTGATCCATCTGCTGGGTATTGTCGCCGCACTGCATGCGCTGt                                                  >  3:318726/1‑149 (MQ=255)
                                                                                                          aGGCGGACCGGGAACGTGTTCTGTTACCTCAAGTGTACTCGGCAAGCAGGATGCATATGGATTACCACAGCCCCTACTTCTTTGGTTACCTACTCGGCCTGATCCATCTGCTGGGTATTGTCGCCGCACTGCATGCGCTGTTCACCGTg                                          >  6:19613/1‑149 (MQ=255)
                                                                                                              ggACCGGGAACGTGTTCTGTTACCTCAAGTGTACTCGGCAAGCAGGATGCATATGGATTACCACAGCCCCTACTTCTTTGGTTACCTACTCGGCCTGATCCATCTGCTGGGTATTGTCGCCGCACTGCATGCGCTGTTCACCGTGCGGa                                      >  1:293171/1‑149 (MQ=255)
                                                                                                                    ggAACGTGTTCTGTTACCTCAAGTGTACTCGGTAAGCAGGATGCATATGGATTACCACAGCCCCTACTTCTTTGGTTACCTACTCGGCCTGATCCATCTGCTGGGTATTGTCGCCGCACTGCATGCGCTGTTCACCGTGCGGACAGcc                                 >  6:13011/1‑148 (MQ=255)
                                                                                                                          tgtTCTGTTACCTCAAGTGTACTCGGTAAGCAGGATGCATATGGATTACCACAGCCCCTACTTCTTTGGTTACCTACTCGGCCTGATCCATCTGCTGGGTATTGTCGCCGCACTGCATGCGCTGTTCACCGTGCGGACAGCCCAGGgcg                          <  5:13011/149‑1 (MQ=255)
                                                                                                                                gTTACCTCAAGTGTACTCGGCAAGCAGGATGCATATGGATTACCACAGCCCCTACTTCTTTGGTTACCTACTCGGCCTGATCCATCTGCTGGGTATTGTCGCCGCACTGCATgcgc                                                     >  3:223988/1‑116 (MQ=255)
                                                                                                                                gTTACCTCAAGTGTACTCGGCAAGCAGGATGCATATGGATTACCACAGCCCCTACTTCTTTGGTTACCTACTCGGCCTGATCCATCTGCTGGGTATTGTCGCCGCACTGCATgcgc                                                     <  4:223988/116‑1 (MQ=255)
                                                                                                                                gTTACCTCAAGTGTACTCGGCAAGCAGGATGCATATGGATTACCACAGCCCCTACTTCTTTGGTTACATACTCGGCCTGATCCATCTGCTGGGTATTGTCGCCGCACTGCATGCGCTGTTCACCGTGCGGACAGCCCAGGGCGCCAtt                     >  5:242196/1‑148 (MQ=255)
                                                                                                                                 ttACCTCAAGTGTACTCGGCAAGCAGGATGCATATGGATTACCACAGCCCCTACTTCTTTGGTTACCTACTCGGCCTGATCCATCTGCTGGGTATTGTCGCCGCACTGCATGCGCTGTTCACCGTGCGGACAGCCCAGGGCGCCAt                      <  6:351822/146‑1 (MQ=255)
                                                                                                                                     cTCAAGTGTACTCGGCAAGCAGGATGCATATGGATTACCACAGCCCCTACTTCTTTGGTTACCTACTCGGCCTGATCCATCTGCTGGGTATTGTCGCCGCACTGCATGCGCTGTTCACCGTGCGGACAGCCCAGGGCGCCATTGCTTgg               <  1:184909/149‑1 (MQ=255)
                                                                                                                                     cTCAAGTGTACTCGGCAAGCAGGATGCATATGGATTACCACAGCCCCTACTTCTTTGGTTACCTACTCGGCCTGATCCATCTGCTGGGTATTGTCGCCGCACTGCATGCGCTGTTCACCGTGCGGACAGCCCAGGGCGCCATTGCTTgg               >  5:45386/1‑149 (MQ=255)
                                                                                                                                     cTCAAGTGTACTCGGCAAGCAGGATGCATATGGATTACCACAGCCCCTACTTCTTTGGTTACCTACTCGGCCTGATCCATCTGCTGGGTATTGTCGCCGCACTGCATGCGCTGTTCACCGTGCGGACAGCCCAGGGCGCCATTGCTTgg               >  3:207655/1‑149 (MQ=255)
                                                                                                                                        aaGTGTACTCGGCAAGCAGGATGCATATGGATTACCACAGCCCCTACTTCTTTGGTTACCTACTCGGCCTGATCCATCTGCTGGGTATTGTCGCCGCACTGCATGCGCTGTTCACCGTGCGGACAGCCCAGGGCGCCATTGCTTGGGc             >  4:193982/1‑148 (MQ=255)
                                                                                                                                          gtgtACTCGGCAAGCAGGATGCATATGGATTACCACAGCCCCTACTTCTTTGGTTACCTACTCGGCCTGATCCATCTGCTGGGTATTGTCGCCGCACTGCATGCGCTGTTCACCGTGCGGACAGc                                  <  4:288804/125‑1 (MQ=255)
                                                                                                                                          gtgtACTCGGCAAGCAGGATGCATATGGATTACCACAGCCCCTACTTCTTTGGTTACCTACTCGGCCTGATCCATCTGCTGGGTATTGTCGCCGCACTGCATGCGCTGTTCACCGTGCGGACAGc                                  >  3:288804/1‑125 (MQ=255)
                                                                                                                                          gtgtACTCGGCAAGCAGGATGCATATGGATTACCACAGCCCCTACTTCTTTGGTTACCTACTCGGCCTGATCCATCTGCTGGGTATTGTCGCCGCACTGCATGCGCTGTTCACCGTGCGGACAGCCCAGGGCGCCATTGCTTGGGTGAt          <  8:114085/149‑1 (MQ=255)
                                                                                                                                          gtgtACTCGGCAAGCAGGATGCATATGGATTACCACAGCCCCTACTTCTTTGGTTACCTACTCGGCCTGATCCATCTGCTGGGTATTGTCGCCGCACTGCATGCGCTGTTCACCGTGCGGACAGCCCAGGGCGCCATTGCTTGGGCGAt          <  3:193982/149‑1 (MQ=255)
                                                                                                                                              aCTCGGCAAGCAGGATGCATATGGATTACCACAGCCCCTACTTCTTTGGTTACCTACTCGGCCTGATCCATCTGCTGGGTATTGTCGCCGCACTGCATGCGCTGTTCACCGTGCGGACAGTCCAGGGCGCCATTGCTTggg              <  4:190762/141‑1 (MQ=255)
                                                                                                                                              aCTCGGCAAGCAGGATGCATATGGATTACCACAGCCCCTACTTCTTTGGTTACCTACTCGGCCTGATCCATCTGCTGGGTATTGTCGCCGCACTGCATGCGCTGTTCACCGTGCGGACAGTCCAGGGCGCCATTGCTTggg              >  3:190762/1‑141 (MQ=255)
                                                                                                                                               cTCGGCAAGCAGGATGCATATGGATTACCACAGCCCCTACTTCTTTGGTTACCTACTCGGCCTGATCCATCTGCTGGGTATTGTCGCCGCACTGCATGCGCTGTTCACCGTGCGGACAGCCCAGGGCGCCATTGCTTGGGCGATGccc      <  3:13319/148‑1 (MQ=255)
                                                                                                                                                  ggCAAGCAGGATGCATATGGATTACCACAGCCCCTACTTCTTTGGTTACCTACTCGGCCTGATCCATCTGCTGGGTATTGTCGCCTCACTGCATGCGCTGTTCACCGTGCGGACAGCCCAGGGCGCCATTGCTTGGGCGATGCCCTTGc  <  7:244594/149‑1 (MQ=255)
                                                                                                                                                  ggCAAGCAGGATGCATATGGATTACCACAGCCCCTACTTCTTTGGTTACCTACTCGGCCTGATCCATCTGCTGGGTATTGTCGCCGCACTGCATGCGCTGTTCACCGTGCGGACAGCCCAGGGCGCCATTGCTTGGGCGATGCCCtt    <  5:19613/147‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CAACGCCAACAGTGGCAGACGAATCTTCATGGACGAACGCTCCTGGAAAAAAGCCGGTCAAACGGGCCGGACTGAGCCTTAGACGCCAAATCCGCCTGCGGGTTGCAGGCGGACCGGGAACGTGTTCTGTTACCTCAAGTGTACTCGGCAAGCAGGATGCATATGGATTACCACAGCCCCTACTTCTTTGGTTACCTACTCGGCCTGATCCATCTGCTGGGTATTGTCGCCGCACTGCATGCGCTGTTCACCGTGCGGACAGCCCAGGGCGCCATTGCTTGGGCGATGCCCTTGC  >  NC_002947/6112715‑6113009

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑