Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,132,135 C→G 7.2% G573A (GGC→GCC)  copA‑II ← copper resistance protein A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,132,1350CG 7.2% 206.5 / 6.0 69G573A (GGC→GCC) copA‑IIcopper resistance protein A
Reads supporting (aligned to +/- strand):  ref base C (33/31);  new base G (2/3);  total (35/34)
Fisher's exact test for biased strand distribution p-value = 6.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.82e-01

AATTACCGTCCTCATCCTCCAGATCGCTCCACATTCCGTGGAGGTGGATGGGGTGAGTCATCATGGTGTCGTTGACCAAAGTGATTCGCAGACGCTCGCCATATTTGAGGCGTAGGGGTTCAGCATCGGAAAATTTGACGCCGTCAAATGACCAGGCGAACTTCTCCATATGGCCTGTGAGATGCAGCTCGATGGTGCGCCCTGGTTCACGTCCATCTGGATCAATGAACGTGCTTCGCAGATCCGCATAGGTCAGGACACGACGTCCATTGTTGCG  >  NC_002947/6131995‑6132271
                                                                                                                                            |                                                                                                                                        
aaTTACCGTCCTCATCCTCCAGATCGCTCCACATTCCGTGGAGGTGGATGGGGTGAGTCATCATGGTGTCGTTGACCAAAGTGATTCGCAGACGCTCGCCATATTTGAGGCGTAGGGGTTCAGCATCGGAAAATTTGACGCCGTCaaa                                                                                                                                   <  4:226619/148‑1 (MQ=255)
  ttACCGTCCTCATCCTCCAGATCGCTCCACATTCCGTGGAGGTGGATGGGGTGAGTCATCATGGTGTCGTTGACCAAAGTGATTCGCAGACGCTCGCCATATTTGAGGCGTAGGGGTTCAGCATCGGAAAATTTGACGGCGTCAAAttt                                                                                                                                <  2:312521/149‑3 (MQ=255)
     ccGTCCTCATCCTCCAGATCGCTCCACATTCCGTGGAGGTGGATGGGGTGAGTCATCATGGTGTCGTTGACCAAAGTGATTCGCAGACGCTCGCCATATTTGAGGCGTAGGGGTTCAGCATCGGAAAATTTGACGCCGTCAAATGAcc                                                                                                                              <  2:153535/148‑1 (MQ=255)
      cGTCCTCATCCTCCAGATCGCTCCACATTCCGTGGAGGTGGATGGGGTGAGTCATCATGGTGTCGTTGACCAAAGTGATTCGCAGACGCTCGCCATATTTGAGGCGTAGGGGTTCAGCATCGGAAAATTTGACGCCGTCAAATGACCAg                                                                                                                            >  8:299627/1‑149 (MQ=255)
        tCCTCATCCTCCAGATCGCTCCACATTCCGTGGAGGTGGATGGGGTGAGTCATCATGGTGTCGTTGACCAAAGTGATTCGCAGACGCTCGCCATATTTGAGGCGTAGGGGTTCAGCATCGGAAAATTTGACGCCGTCAAATGACCAGGc                                                                                                                          >  1:274301/1‑149 (MQ=255)
           tCATCCTCCAGATCGCTCCACATTCCGTGGAGGTGGATGGGGTGAGTCATCATGGTGTCGTTGACCAAAGTGATTCGCAGACGCTCGCCATATTTGAGGCGTAGGGGTTCAGCATCGGAAAATTTGACGCCGTCAAATGACCAGGCGaa                                                                                                                       >  7:107281/1‑149 (MQ=255)
            cATCCTCCAGATCGCTCCACATTCCGTGGAGGTGGATGGGGTGAGTCATCATGGTGTCGTTGACCAAAGTGATTCGCAGACGCTCGCCATATTTGAGGCGTAGGGGTTCAGCATCGGAAAATTTGACGCCGTCAAATGACCAGGCGAAc                                                                                                                      >  8:28579/1‑149 (MQ=255)
             aTCCTCCAGATCGCTCCACATTCCGTGGAGGTGGATGGGGTGAGTCATCATGGTGTCGTTGACCAAAGTGATTCGCAGACGCTCGCCATATTTGAGGCGTAGGGGTTCAGCATCGGAAAATTTGACGCCGTCAAATGACCAGGCGAAc                                                                                                                      <  2:29617/148‑1 (MQ=255)
             aTCCTCCAGATCGCTCCACATTCCGTGGAGGTGGATGGGGTGAGTCATCATGGTGTCGTTGACCAAAGTGATTCGCAGACGCTCGCCATATTTGAGGCGTAGGGGTTCAGCATCGGAAAATTTGACGCCGTCAAATGACCAGGCGAACt                                                                                                                     >  5:336507/1‑149 (MQ=255)
             aTCCTCCAGATCGCTCCACATTCCGTGGAGGTGGATGGGGTGAGTCATCATGGTGTCGTTGACCAAAGTGATTCGCAGACGCTCGCCATATTTGAGGCGTAGGGGTTCAGCATCGGAAAATTTGACGCCGTCAAATGACCAGGCGAACt                                                                                                                     >  4:107125/1‑149 (MQ=255)
             aTCCTCCAGATCGCTCCACATTCCGTGGAGGTGGATGGGGTGAGTCATCATGGTGTCGTTGACCAAAGTGATTCGCAGACGCTCGCCATATTTGAGGCGTAGGGGTTCAGCATCGGAAAATTTGACGCCGTCAAATGACCAGGCGAACt                                                                                                                     >  4:124691/1‑149 (MQ=255)
                 tccAGATCGCTCCACATTCCGTGGAGGTGGATGGGGTGAGTCATCATGGTGTCGTTGACCAAAGTGATTCGCAGACGCTCGCCATATTTGAGGCGTAGGGGTTCAGCATCGGAAAATTTGACGCCGTCAAATGACCAGGCGAACTtctc                                                                                                                 >  6:83112/1‑149 (MQ=255)
                  ccAGATCGCTCCACATTCCGTGGAGGTGGATGGGGTGAGTCATCATGGTGTCGTTGACCAAAGTGATTCGCAGACGCTCGCCATATTTGAGGCGTAGGGGTTCAGCATCGGAAAATTTGACGCCGTCAAATGACCAGGCGAACTTCTcc                                                                                                                <  3:38874/149‑1 (MQ=255)
                   cAGATCGCTCCACATTCCGTGGAGGTGGATGGGGTGAGTCATCATGGTGTCGTTGACCAAAGTGATTCGCAGACGCTCGCCATATTTGAGGCGTAGGGGTTCAGCATCGGAAAATTTGACGCCGTCAAATGACCAGGCGAACTTCTcc                                                                                                                <  3:124691/148‑1 (MQ=255)
                   cAGATCGCTCCACATTCCGTGGAGGTGGATGGGGTGAGTCATCATGGTGTCGTTGACCAAAGTGATTCGCAGACGCTCGCCATATTTGAGGCGTAGGGGTTCAGCATCGGAAAATTTGACGCCGTCAAATGACCAGGCGAACTTCTCCa                                                                                                               <  6:336507/149‑1 (MQ=255)
                         gCTCCACATTCCGTGGAGGTGGATGGGGTGAGTCATCATGGTGTCGTTGACCAAAGTGATTCGCAGACGCTCGCCATATTTGAGGCGTAGGGGTTCAGCATCGGAAAATTTGACGCCGTCAAATGACCAGGCGAACTTCTCCATATGGc                                                                                                         <  8:254717/149‑1 (MQ=255)
                              acaTTCCGTGGAGGTGGATGGGGTGAGTCATCATGGTGTCGTTGACCAAAGTGATTCGCAGACGCTCGCCATATTTGAGGCGTAGGGGTTCAGCATCGGAAAATTTGACGCCGTCAAATGACCAGGCGAACTTCTCCATATGGCCtgtg                                                                                                    <  4:155954/149‑1 (MQ=255)
                                 ttCCGTGGAGGTGGATGGGGTGAGTCATCATGGTGTCGTTGACCAAAGTGATTCGCAGACGCTCGCCATATTTGAGGCGTAGGGGTTCAGCATCGGAAAATTTGACGCCGTCAAATGACCAGGCGAACTTCTCCATATGGCCTGTgaga                                                                                                 <  2:274301/149‑1 (MQ=255)
                                 ttCCGTGGAGGTGGATGGGGTGAGTCATCATGGTGTCGTTGACCAAAGTGATTCGCAGACGCTCGCCATATTTGAGGCGTAGGGGTTCAGCATCGGAAAATTTGACGCCGTCAAATGACCAGGCGAACTTCTCCATATGGCCTGTgaga                                                                                                 <  3:107125/149‑1 (MQ=255)
                                    cGTGGAGGTGGATGGGGTGAGTCATCATGGTGTCGTTGACCAAAGTGATTCGCAGACGCTCGCCATATTTGAGGCGTAGGGGTTCAGCATCGGAAAATTTGACGCCGTCAAATGACCAGGCGAACTTCTCCATATGGCCTGTGAGATGc                                                                                              <  8:107281/149‑1 (MQ=255)
                                     gTGGAGGTGGATGGGGTGAGTCATCATGGTGTCGTTGACCAAAGTGATTCGCAGACGCTCGCCATATTTGAGGCGTAGGGGTTCAGCATCGGAAAATTTGACGCCGTCAAATGACCAGGCGAACTTCTCCATATGGCCTGTGAGATg                                                                                               >  8:186705/1‑147 (MQ=255)
                                       ggAGGTGGATGGGGTGAGTCATCATGGTGTCGTTGACCAAAGTGATTCGCAGACGCTCGCCATATTTGAGGCGTAGGGGTTCAGCATCGGAAAATTTGACGCCGTCAAATGACCAGGCGAACTTCTCCATATGGCCTGTGAGATGCAGc                                                                                           >  5:137659/1‑149 (MQ=255)
                                                         tcatcaTGGTGTCGTTGACCAAAGTGATTCGCAGACGCTCGCCATATTTGAGGCGTAGGGGTTCAGCATCGGAAAATTTGACGCCGTCAAATGACCAGGCGAACTTCTCCATATGGCCTGTGAGATGCAGCTCGATGGTGCGCCCTGGt                                                                         <  1:22044/149‑1 (MQ=255)
                                                           aacatCGTGTCGTTGACCAAAGTGATTCGCAGACGCTCGCCATATTTGAGGCGTACTGGATCAGCATCGGAAAATTTCACCCCGTCAAATGACCAGGCCAACTTCTACATAAGTCCTGTGAGAAGCAGCTCGATGGTGAGCCCTgaat                                                                        >  8:288117/3‑145 (MQ=255)
                                                            tcatGGTGTCGTTGACCAAAGTGATTCGCAGACGCTCGCCATATTTGAGGCGTAGGGGTTCAGCATCGGAAAATTTGACGCCGTCAAATGACCAGGCGAACTTCTCCATATGGCCTGTGAGATGCAGCTCGATGGTGCGCCCTGGTTCa                                                                      >  6:272245/1‑149 (MQ=255)
                                                              atGGTCTCGTTGACCAAAGTGATTCGCAGACGCTCGCCATATTTGAGGCGTAGGGGTTCAGCATCGGAAAATTTGACGCCGTCAAATGACCAGGCGAACTTCTCCATATGGCATGTAAGATGCAGCTCGATGGTGCGCCCTGGTacac                                                                     >  8:13892/1‑148 (MQ=255)
                                                               tGGTGTCGTTGACCAAAGTGATTCGCAGACGCTCGCCATATTTGAGGCGTAGGGGTTCAGCATCGGAAAATTTGACGCCGTCAAATGACCAGGCGAACTTCTCCATATGGCCTGTGAGATGCAGCTCGATGGTGCGCCCTGGTTCACGt                                                                   <  5:272245/149‑1 (MQ=255)
                                                                ggTGTCGTTGACCAAAGTGATTCGCAGACGCTCGCCATATTTGAGGCGTAGGGGTTCAGCATCGGAAAATTTGACGCCGTCAAATGACCAGGCGAACTTCTCCATATGGCCTGTGAGATGCAGCTCGATGGTGCGCCCTGGTTCACGTc                                                                  >  2:229349/1‑149 (MQ=255)
                                                                  tgtCGTTGACCAAAGTGATTCGCAGACGCTCGCCATATTTGAGGCGTAGGGGTTCAGCATCGGAAAATTTGACGCCGTCAAATGACCAGGCGAACTTCTCCATATGGTCTGTGAGATGCAGCTCGATGGTGCGCCCTGGTTCACGTCCa                                                                <  5:83112/149‑1 (MQ=255)
                                                                   gtCGTTGACCAAAGTGATTCGCAGACGCTCGCCATATTTGAGGCGTAGGGGTTCAGCATCGGAAAATTTGACGCCGTCAAATGACCAGGCGAACTTCTCCATATGGCCTGTGAGATGCAGCTCGATGGTGCGCCCTGGTTCACGTCCa                                                                >  4:135171/1‑148 (MQ=255)
                                                                   gtCGTTGACCAAAGTGATTCGCAGACGCTCGCCATATTTGAGGCGTAGGGGTTCAGCATCGGAAAATTTGACGCCGTCAAATGACCAGGCGAACTTCTCCATATGGCCTGTGAGATGCAGCTCGATGGTGCGCCCTGGTTCACGTCCa                                                                >  4:16096/1‑148 (MQ=255)
                                                                       ttGACCAAAGTGATTCGCAGACGCTCGCCATATTTGAGGCGTAGGGGTTCAGCATCGGAAAATTTGACGCCGTCAAATGACCAGGCGAACTTCTCCATATGGCCTGTGAGATGCAGCTCGATGGTGCGCCCTGGTTCACGTCCATCTg                                                            >  4:234734/1‑148 (MQ=255)
                                                                         gACAAAAGTGATTCGCAGACGCTCGCCATATTTGAGGAGTAGTGGTTCAGCATCGGAAAATTTGACGCCGTCAAATGACCAGGCGAACTTCTCCATATGGACTGTGAGATGCAGCTCGATGGTGCGCCATGGTTCACGTCCATCTgta                                                          >  4:60166/1‑146 (MQ=255)
                                                                           ccAAAGTGATTCGCAGACGCTCGCCATATTTGAGGCGTAGGGGTTCAGCATCGGAAAATTTGACGCCGTCAAATGACCAGGCGAACTTCTCCATATGGCCTGTGAGATGCAGCTCGATGGTGCGCCCTGGTTCACGTCCATCTGGATc                                                        <  6:137659/148‑1 (MQ=255)
                                                                              aaGTGATTCGCAGACGCTCGCCATATTTGAGGCGTAGGGGTTCAGCATCGGAAAATTTGACGCCGTCAAATGACCAGGCGAACTTCTCCATATGGCCTGTGAGATGCAGCTAGATGGTGCGCACTGGTTCACGTCCATCTGGATCAAt                                                     >  3:49954/1‑148 (MQ=255)
                                                                                  gATTCGCAGACGCTCGCCATATTTGAGGCGTAGGGGTTCAGCATCGGAAAATTTGACGCCGTCAAATGACCAGGCGAACTTCTCCATATGGCCtgt                                                                                                     >  2:371350/1‑96 (MQ=255)
                                                                                  gATTCGCAGACGCTCGCCATATTTGAGGCGTAGGGGTTCAGCATCGGAAAATTTGACGCCGTCAAATGACCAGGCGAACTTCTCCATATGGCCtgt                                                                                                     <  1:371350/96‑1 (MQ=255)
                                                                                     tCGCAGACGCTCGCCATATTTGAGGCGTAGGGGTTCAGCATCGGAAAATTTGACGCCGTCAAATGACCAGGCGAACTTCTCCATATGGCCTGTGAGATGCAGCTCGATGGTGCGCCCTGGTTCACGTCCATCTGGATCAATGAACGTGc                                             >  1:329851/1‑149 (MQ=255)
                                                                                       gctgACGCTCGCCATATTTGAGGCGTAGGGGTTCAGCATCGGAAAATTTGACGCCGTCAAATGACCAGGCGAACTTCTCCATATGGCCTGTGAGATGCAGCTCGATGGTGCGCCCTGGTTCACGTCCATCTGGATCAATGAACGTGCtt                                           <  7:288117/146‑1 (MQ=255)
                                                                                       gCAGACGCTCGCCATATTTGAGGCGTAGGGGTTCAGCATCGGAAAATTTGACGCCGTCAAATGACCAGGCGAACTTCTCCATATGGCCTGTgaga                                                                                                 <  7:240975/95‑1 (MQ=255)
                                                                                       gCAGACGCTCGCCATATTTGAGGCGTAGGGGTTCAGCATCGGAAAATTTGACGCCGTCAAATGACCAGGCGAACTTCTCCATATGGCCTGTgaga                                                                                                 >  8:240975/1‑95 (MQ=255)
                                                                                         aGACGCTCGCCATATTTGAGGCGTAGGGGTTCAGCATCGGAAAATTTGACGCCGTCAAATGACCAGGCGAACTTCTCCATATGGCCTGTGAGATGCAGCTCGATGGTGCGCCCTGGTTCACGTCCATCTGGATCAATGAACGTGCTTCg                                         >  3:264064/1‑149 (MQ=255)
                                                                                         aGACGCCCGCCATATTTGAGGCGTAGGGGTTCAGCATCGGAAAATTTGACGCCGTCAAATGACCAGGCGAACTTCTCCATATGGc                                                                                                         <  5:63750/85‑1 (MQ=255)
                                                                                         aGACGCACGCCATATTTGAGGCGTAGGGGTTCAGCATCGGAAAATTTGACGCCGTCAAATGACCAGGCGAACTTCTCCATATGGc                                                                                                         >  6:63750/1‑85 (MQ=255)
                                                                                           aCGCTCGCCATATTTGAGGCGTAGGGGTTCAGCATCGGAAAATTTGACGCCGTCAAATGACCAGGCGAACTTCTCCATATGGCCTGTGAGATGCAGCTCGATGGTGCGCCCTGGTTCACGTCCATCTGGATCAATGAACGTGCTTCg                                         >  3:115463/1‑147 (MQ=255)
                                                                                               tCGCCATATTTGAGGCGTAGGGGTTCAGCATCGGAAAATTTGACGCCGTCAAATGACCAGGCGAACTTCTCCATATGGCCTGTGAGATGCAGCTCGATGGTGCGCCCTGGTTCACGTCCATCTGGATCAATGAACGTGCTTCGCAGATc                                   >  7:212036/1‑149 (MQ=255)
                                                                                                 gCCATATTTGAGGCGTAGGGGTTCAGCATCGGAAAATTTGACGCCGTCAAATGACCAGGCTATCTTCTCCATATGGCCTGTGAGATGCAGCTCGATGGTGCGCCCTGGTTCACGTCCATCTGGATCAATGAACGTGCTTCGCAGATc                                   <  6:74756/147‑1 (MQ=255)
                                                                                                 gCCATATTTGAGGCGTAGGGGTTCAGCATCGGAAAATTTGACGCCGTCAAATGACCAGGCGAACTTCTCCATATGGCCTGTGAGATGCAGCTCGATGGTGCGCCCCGGTTCACGTCCATCTGGATCAATGAACGTGCt                                            <  7:49762/138‑1 (MQ=255)
                                                                                                 gCCATATTTGAGGCGTAGGGGTTCAGCATCGGAAAATTTGACGCCGTCAAATGACCAGGCGAACTTCTCCATATGGCCTGTGAGATGCAGCTCGATGGTGCGCCCCGGTTCACGTCCATCTGGATCAATGAACGTGCt                                            >  8:49762/1‑138 (MQ=255)
                                                                                                  ccATATTTGAGGCGTAGGGGTTCAGCATCGGAAAATTTGACGCCGTCAAATGACCAGGCGAACTTCTCCATATGGCCTGTGAGATGCAGCTCGATGGTGCGCCCTGGTTCACGTCCATCTGGATCAATGAACGTGCTTCGCAGATCCGc                                <  7:13892/149‑1 (MQ=255)
                                                                                                   cATATTTGAGGCGTAGGGGTTCAGCATCGGAAAATTTGACGCCGTCAAATGACCAGGCGAACTTCTCCATATGGCCTGTGAGATGCAGCTCGATGGTGCGCCCTGGTTCACGTCCATCTGGATCAATGAACGTGCTTCGCAGATCCGCa                               <  7:28579/149‑1 (MQ=255)
                                                                                                       tttGAGGCGTAGGGGTTCAGCATCGGAAAATTTGACGCCGTCAAATGACCAGGCGAACTTCTCCATATGGCCTGTGAGATGCAGCTCGATGGTGCGCCCTGGTTCACGTCCATCTGGATCAATGAACGTGCTTCGCAGATCCGCATAg                            <  7:299627/148‑1 (MQ=255)
                                                                                                       tttGAGGCGTAGGGGTTCAGCATCGGAAAATTTGACGCCGTCAAATGACCAGGCGAACTTCTCCATATGGCCTGTGAGATGCAGCTCGATGGTGCGCCCTGGTTCACGTCCATCTGGATCAATGAACGTGCTTCGCAGATCCGCATAg                            <  8:212036/148‑1 (MQ=255)
                                                                                                          gAGGCGTAGGGGTTCAGCATCGGAAAATTTGACGCCGTCAAATGACCAGGCGAACTTCTCCATATGGCCTGTGAGATGCAGCTCGATGGTGCGCCCTGGTTCACGTCCATCTGGATCAATGAACGTGCTTCGCAGATGCGCATAGGTc                         <  4:264064/148‑1 (MQ=255)
                                                                                                               gTAGGGGTTCAGCATCGGAAAATTTGACGCCGTCAAATGACCAGGCGAACTTCTCCATATGGCCTGTGAGATGCAGCTCGATGGTGCGCCCTGGTTCACGt                                                                   <  6:62607/101‑1 (MQ=255)
                                                                                                               gTAGGGGTTCAGCATCGGAAAATTTGACGCCGTCAAATGACCAGGCGAACTTCTCCATATGGCCTGTGAGATGCAGCTCGATGGTGCGCCCTGGTTCACGt                                                                   >  5:62607/1‑101 (MQ=255)
                                                                                                                  ggggTTCAGCATCGGAAAATTTGACGCCGTCAAATGACCAGGCGAACTTCTCCATATGGCCTGTGAGATGCAGCTCGATGGTGCGCCCTGGTTCACGTCCATCTGGATCAATGAACGTGCTTCGCAGATCCGCATAGGTCAGGACacga                >  5:249397/1‑149 (MQ=255)
                                                                                                                    ggTTCAGCATCGGAAAATTTGACGCCGTCAAATGACCAGGCGAACTTCTCCATATGGCCTGTGAGATGCAGCTCGATGGTGCGCCCTGGTTCACGTCCATCTGGATCAATGAACGTGCTTCGCAGATCCGCATAGGTCAGGACacgacg              >  1:16533/1‑149 (MQ=255)
                                                                                                                     gTTCAGCATCGGAAAATTTGACGCCGTCAAATGACCAGGCGAACTTCTCCATATGGCCTGTGAGATGCAGCTCGATGGTGCGCCCTGGTTCACGTCCATCTGGATCAATGAACGTGCTTCGCAGATCCGCATAGGTCAGGACACGACGt             >  6:178421/1‑149 (MQ=255)
                                                                                                                     gTTCAGCATCGGAAAATTTGACGCCGTCAAATGACCAGGCGAACTTCTCCATATGGCCTGTGAGATGCAGCTCGATGGTGCGCCCTGGTTCACGTCCATCTGGATCAATGAACGTGCTTCGCAGATCCGCATAGGTCAGGACACGACGt             <  3:16096/149‑1 (MQ=255)
                                                                                                                             tCGGAAAATTTGACTCCGTCAAATGACCATGCGAACTTCTCCATATGGACTGTGAGATGCAGCTCGATTTTGCGCCCTGGTTCACGTCCATCTGGATCAATGAACTTGCTTCTCAGATCCGCATAGGTCAGGACACGACGTCCAttgtt     <  3:60166/149‑1 (MQ=255)
                                                                                                                             tCGGAAAATTTGACGCCGTCAAATGACCAGGCGAACTTCTCCATATGGCCTGTGAGATGCAGCTCGATGGTGCGCCCTGGTTCACGTCCATCTGGATCAATGAACGTGCTTCGCa                                       <  1:105002/115‑1 (MQ=255)
                                                                                                                             tCGGAAAATTTGACGCCGTCAAATGACCAGGCGAACTTCTCCATATGGCCTGTGAGATGCAGCTCGATGGTGCGCCCTGGTTCACGTCCATCTGGATCAATGAACGTGCTTCGCa                                       >  2:105002/1‑115 (MQ=255)
                                                                                                                             tCGGAAAATTTGACGCCGTCAAATGACCAGGCGAACTTCTCCATATGGCCTGTGAGATGCAGCTCGATGGTGCGCCCTGGTTCACGTCCATCTGGATCAATGAACGTGCTTCGCAGATCCGCATAGGTCAGGACACGACGTCCAttgtt     <  4:115463/149‑1 (MQ=255)
                                                                                                                               tggtcaTTTGACGGCGTCAAATGACCAGGCGAACTTCTCCATATGGCCTGTGAGATGCAGCTCGATGGTGCGCCCTGGTTCACGTCCTTCTGGATCAATGAACGTGCTTCGCAGATcc                                  <  1:33897/113‑1 (MQ=255)
                                                                                                                               tggtcaTTTGACGGCGTCAAATGACCAGGCGAACTTCTCCATATGGCCTGTGAGATGCAGCTCGATGGTGCGCCCTGGTTCACGTCCATCTGGATCAATGAACGTGCTTCGCAGATcc                                  >  2:33897/6‑118 (MQ=255)
                                                                                                                                gAAAATTTGACGCCGTCAAATGACCAGGCGAACTTCTCCATATGGCCTGTGAGATGCAGCTCGATGGTGCGCCCTGGTTCACGTCCATCTGGATCAATGAACGTGCTTCGCAGATCCGCATAGGTCAGGACACGACGTCCATTGTTgcg  >  3:365788/1‑149 (MQ=255)
                                                                                                                                 gtcaTTTGACGGCGTCAAATGACCAGGCGAACTTCTCCATATGGCCTGTGAGATGCAGCTCGATGGTGCGCCCTGGTTCACGTCCATCTGGATCAATGAACGTGCTTCGCAGATCCGCATAGGTCAGGACACGACGTCCAttgttg    <  4:132366/143‑1 (MQ=255)
                                                                                                                                 gtcaTTTGACGGCGTCAAATGACCAGGCGAACTTCTCCATATGGCCTGTGAGATGCAGCTCGATGGTGCGCCCTGGTTCACGTCCATCTGGATCAATGAACGTGCTTCGCAGATCCGCATAGGTCAGGACACGACGTCCAttgttg    >  3:132366/4‑146 (MQ=255)
                                                                                                                                            |                                                                                                                                        
AATTACCGTCCTCATCCTCCAGATCGCTCCACATTCCGTGGAGGTGGATGGGGTGAGTCATCATGGTGTCGTTGACCAAAGTGATTCGCAGACGCTCGCCATATTTGAGGCGTAGGGGTTCAGCATCGGAAAATTTGACGCCGTCAAATGACCAGGCGAACTTCTCCATATGGCCTGTGAGATGCAGCTCGATGGTGCGCCCTGGTTCACGTCCATCTGGATCAATGAACGTGCTTCGCAGATCCGCATAGGTCAGGACACGACGTCCATTGTTGCG  >  NC_002947/6131995‑6132271

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑