Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	532,616	G→C	5.8%	noncoding (35/76 nt)	PP_t03 →	tRNA‑Thr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	532,616	0	G	C	5.8%	220.4 / 3.9	69	noncoding (35/76 nt)	PP_t03	tRNA‑Thr
Reads supporting (aligned to +/- strand):  ref base G (37/28);  new base C (2/2);  total (39/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.89e-01

CCGCAAGCTCCGCGGGTATAGTTTAGTGGTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGTTTGATCGTTCCC  >  NC_002947/532468‑532763                                                                                                                                                     |                                                                                                                                                      ccGCAAGCTCCGCGGGTATAGTTTAGTGGTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTgg                                                                                                                                                       >  2:200388/1‑149 (MQ=255)  cGCAAGCTCCGCGGGTATAGTTTAGTGGTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTgg                                                                                                                                                       >  8:166744/1‑148 (MQ=255)    cAAGCTCCGCGGGTATAGTTTAGTGGTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTGCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTaa                                                                                                                                                    <  5:258478/149‑1 (MQ=255)        cTCCGCGGGTATAGTTTAGTGGTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑Gt                                                                                                                                               <  3:354427/149‑1 (MQ=255)         tCCGCGGGTATAGTTTAGTGGTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACACTTGGTAAGG‑Gt                                                                                                                                               >  3:97072/1‑148 (MQ=255)           cgcgGGTATAGTTTAGTGGTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAg                                                                                                                                            <  2:87963/149‑1 (MQ=255)             cgGGTATAGTTTAGTGGTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGt                                                                                                                                          >  8:101729/1‑149 (MQ=255)             cgGGTATAGTTTAGTGGTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGt                                                                                                                                          <  1:6062/149‑1 (MQ=255)             cgGGTATAGTTTAGTGGTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTACCAAGG‑GTgtgct                                                                                                                                          <  3:47506/149‑5 (MQ=255)                      tttAGTGGTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGtt                                                                                                                                  <  6:267379/148‑1 (MQ=255)                             gTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCg                                                                                                                          >  2:78328/1‑149 (MQ=255)                             gTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCg                                                                                                                          >  1:258136/1‑149 (MQ=255)                             gTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCg                                                                                                                          >  2:220146/1‑149 (MQ=255)                                     tCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAagag                                                                                                                  >  7:138400/1‑149 (MQ=255)                                        gCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTc                                                                                                               <  2:258136/149‑1 (MQ=255)                                           ttCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCa                                                                                                             >  1:274505/1‑148 (MQ=255)                                              cAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCatat                                                                                                          >  2:139265/1‑148 (MQ=255)                                               aaGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATaa                                                                                                        <  1:200388/149‑1 (MQ=255)                                                aGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATaaa                                                                                                       <  7:101729/149‑1 (MQ=255)                                                     atgatgCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑gg                                                                                                 >  3:263408/1‑149 (MQ=255)                                                      tgatgCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGc                                                                                                <  8:138400/149‑1 (MQ=255)                                                      tgatgCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGAAGAGCACACCCTTCGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGATCCATATAAACAA‑gg                                                                                                 >  6:264998/1‑148 (MQ=255)                                                                  gATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGaaaa                                                                                     >  3:192986/1‑148 (MQ=255)                                                                   aTTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAata                                                                                   >  8:162321/1‑149 (MQ=255)                                                                          cTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGc                                                                                                <  1:206302/129‑1 (MQ=255)                                                                          cTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGc                                                                                                >  2:206302/1‑129 (MQ=255)                                                                             cccGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTg                                                                         >  2:300457/1‑149 (MQ=255)                                                                              ccGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGt                                                                        >  3:156967/1‑149 (MQ=255)                                                                              ccGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGt                                                                        <  6:329848/149‑1 (MQ=255)                                                                                    cAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGc                                                                              <  6:224079/137‑1 (MQ=255)                                                                                    cAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGc                                                                              >  5:224079/1‑137 (MQ=255)                                                                                      aGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAg                                                                <  2:274505/149‑1 (MQ=255)                                                                                       gTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGt                                                               <  4:192986/149‑1 (MQ=255)                                                                                            tCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGc                                                             >  8:264102/1‑146 (MQ=255)                                                                                               gATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACt                                                       <  7:166744/149‑1 (MQ=255)                                                                                                   ttGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTAtt                                                    <  6:171174/148‑1 (MQ=255)                                                                                                     gCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGt                                                                        >  8:69393/1‑126 (MQ=255)                                                                                                     gCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGt                                                                        <  7:69393/126‑1 (MQ=255)                                                                                                       acaAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATttcttc                                               <  7:264102/149‑1 (MQ=255)                                                                                                         aaaGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCATGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTg                                             >  5:329233/1‑149 (MQ=255)                                                                                                         aaaGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTg                                             >  7:161047/1‑149 (MQ=255)                                                                                                          aaGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGc                                            >  6:41479/1‑149 (MQ=255)                                                                                                               tttCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCggag                                        >  6:194723/1‑148 (MQ=255)                                                                                                                ttCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCgga                                         <  4:213859/146‑1 (MQ=255)                                                                                                                ttCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCgga                                         >  3:213859/1‑146 (MQ=255)                                                                                                                  cGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGATCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGAtt                                    >  1:80826/1‑149 (MQ=255)                                                                                                                   gCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCATGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGAtt                                    <  6:329233/148‑1 (MQ=255)                                                                                                                    ctctTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATCTCTTCTGCGGAGGATTg                                   >  4:147895/1‑148 (MQ=255)                                                                                                                     tctTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTa                                 >  7:299203/1‑149 (MQ=255)                                                                                                                       ttGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGAtt                                    >  1:121572/1‑144 (MQ=255)                                                                                                                       ttGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGAtt                                    <  2:121572/144‑1 (MQ=255)                                                                                                                       ttGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATc                               >  8:245766/1‑149 (MQ=255)                                                                                                                       ttGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATc                               >  1:102765/1‑149 (MQ=255)                                                                                                                           aGCTCAGTTGGTAGAGCACACCCTTGGTAAGGTGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGAt                            >  1:44509/1‑149 (MQ=255)                                                                                                                            gCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATgg                          >  2:136737/1‑149 (MQ=255)                                                                                                                            gCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATgg                          <  4:156967/149‑1 (MQ=255)                                                                                                                               cAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCt                        >  3:300654/1‑148 (MQ=255)                                                                                                                                aGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTaa                      <  5:41479/149‑1 (MQ=255)                                                                                                                                 gTTTGTAGAGCACAACCTTGGTAAGG‑GTGAGGTCATCGGTTAAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAg                     <  5:264998/149‑1 (MQ=255)                                                                                                                                     gTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAATTAGAAATTTTAAAATTTATGCCTTTTTTTTATAAGTGCAAGACTATTTATTATGCGGAGGATTTTATCGATGGCTAAGGa                   >  1:115041/1‑148 (MQ=255)                                                                                                                                     gTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGaa                  >  7:34848/1‑148 (MQ=255)                                                                                                                                     gTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGAAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGaa                  >  6:297883/1‑148 (MQ=255)                                                                                                                                       agagCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCTAGACTATTTCTTCTGCGGAGGAtt                                    <  4:244580/128‑1 (MQ=255)                                                                                                                                       agagCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGAtt                                    >  3:244580/1‑128 (MQ=255)                                                                                                                                          gCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGttt            <  7:162321/149‑1 (MQ=255)                                                                                                                                          gCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGttt            <  8:161047/149‑1 (MQ=255)                                                                                                                                           ctcacCCTTACCAAGG‑GTGTGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCaa                                                           >  4:177203/3‑101 (MQ=255)                                                                                                                                           ctcacCCTTACCAAGG‑GTGTGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCaa                                                           <  3:177203/99‑1 (MQ=255)                                                                                                                                           ctcacCCTTACCAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGttt            >  2:102285/3‑148 (MQ=255)                                                                                                                                            acacCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGTTTGa          <  1:136737/149‑1 (MQ=255)                                                                                                                                                  ttGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGTTTGATCGttc    >  2:294637/1‑149 (MQ=255)                                                                                                                                                     gTAAGG‑GTGATGTCATCGGTTCAAGTCAGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGTTTGATCGttccc  <  4:97072/148‑1 (MQ=255)                                                                                                                                                     gTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTAt                                                                   >  4:257458/1‑83 (MQ=255)                                                                                                                                                     gTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTAt                                                                   <  3:257458/83‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                      CCGCAAGCTCCGCGGGTATAGTTTAGTGGTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGTTTGATCGTTCCC  >  NC_002947/532468‑532763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑