Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	532,617	T→C	5.5%	noncoding (36/76 nt)	PP_t03 →	tRNA‑Thr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	532,617	0	T	C	5.5%	217.6 / 2.3	72	noncoding (36/76 nt)	PP_t03	tRNA‑Thr
Reads supporting (aligned to +/- strand):  ref base T (38/30);  new base C (2/2);  total (40/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.92e-01

CAAGCTCCGCGGGTATAGTTTAGTGGTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGTTTGATCGTTCCCTT  >  NC_002947/532471‑532765                                                                                                                                                   |                                                                                                                                                       cAAGCTCCGCGGGTATAGTTTAGTGGTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTGCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTaa                                                                                                                                                      <  5:258478/149‑1 (MQ=255)     cTCCGCGGGTATAGTTTAGTGGTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑Gt                                                                                                                                                 <  3:354427/149‑1 (MQ=255)      tCCGCGGGTATAGTTTAGTGGTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACACTTGGTAAGG‑Gt                                                                                                                                                 >  3:97072/1‑148 (MQ=255)        cgcgGGTATAGTTTAGTGGTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAg                                                                                                                                              <  2:87963/149‑1 (MQ=255)          cgGGTATAGTTTAGTGGTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGt                                                                                                                                            >  8:101729/1‑149 (MQ=255)          cgGGTATAGTTTAGTGGTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGt                                                                                                                                            <  1:6062/149‑1 (MQ=255)          cgGGTATAGTTTAGTGGTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTACCAAGG‑GTgtgct                                                                                                                                            <  3:47506/149‑5 (MQ=255)                   tttAGTGGTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGtt                                                                                                                                    <  6:267379/148‑1 (MQ=255)                          gTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCg                                                                                                                            >  2:220146/1‑149 (MQ=255)                          gTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCg                                                                                                                            >  2:78328/1‑149 (MQ=255)                          gTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCg                                                                                                                            >  1:258136/1‑149 (MQ=255)                                  tCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAagag                                                                                                                    >  7:138400/1‑149 (MQ=255)                                     gCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTc                                                                                                                 <  2:258136/149‑1 (MQ=255)                                        ttCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCa                                                                                                               >  1:274505/1‑148 (MQ=255)                                           cAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCatat                                                                                                            >  2:139265/1‑148 (MQ=255)                                            aaGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATaa                                                                                                          <  1:200388/149‑1 (MQ=255)                                             aGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATaaa                                                                                                         <  7:101729/149‑1 (MQ=255)                                                  atgatgCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑gg                                                                                                   >  3:263408/1‑149 (MQ=255)                                                   tgatgCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGc                                                                                                  <  8:138400/149‑1 (MQ=255)                                                   tgatgCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGAAGAGCACACCCTTCGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGATCCATATAAACAA‑gg                                                                                                   >  6:264998/1‑148 (MQ=255)                                                               gATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGaaaa                                                                                       >  3:192986/1‑148 (MQ=255)                                                                aTTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAata                                                                                     >  8:162321/1‑149 (MQ=255)                                                                       cTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGc                                                                                                  >  2:206302/1‑129 (MQ=255)                                                                       cTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGc                                                                                                  <  1:206302/129‑1 (MQ=255)                                                                          cccGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTg                                                                           >  2:300457/1‑149 (MQ=255)                                                                           ccGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGt                                                                          >  3:156967/1‑149 (MQ=255)                                                                           ccGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGt                                                                          <  6:329848/149‑1 (MQ=255)                                                                                 cAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGc                                                                                <  6:224079/137‑1 (MQ=255)                                                                                 cAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGc                                                                                >  5:224079/1‑137 (MQ=255)                                                                                   aGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAg                                                                  <  2:274505/149‑1 (MQ=255)                                                                                    gTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGt                                                                 <  4:192986/149‑1 (MQ=255)                                                                                         tCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGc                                                               >  8:264102/1‑146 (MQ=255)                                                                                            gATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACt                                                         <  7:166744/149‑1 (MQ=255)                                                                                                ttGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTAtt                                                      <  6:171174/148‑1 (MQ=255)                                                                                                  gCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGt                                                                          >  8:69393/1‑126 (MQ=255)                                                                                                  gCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGt                                                                          <  7:69393/126‑1 (MQ=255)                                                                                                    acaAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATttcttc                                                 <  7:264102/149‑1 (MQ=255)                                                                                                      aaaGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCATGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTg                                               >  5:329233/1‑149 (MQ=255)                                                                                                      aaaGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTg                                               >  7:161047/1‑149 (MQ=255)                                                                                                       aaGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGc                                              >  6:41479/1‑149 (MQ=255)                                                                                                            tttCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCggag                                          >  6:194723/1‑148 (MQ=255)                                                                                                             ttCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCgga                                           <  4:213859/146‑1 (MQ=255)                                                                                                             ttCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCgga                                           >  3:213859/1‑146 (MQ=255)                                                                                                               cGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGATCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGAtt                                      >  1:80826/1‑149 (MQ=255)                                                                                                                gCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCATGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGAtt                                      <  6:329233/148‑1 (MQ=255)                                                                                                                 ctctTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATCTCTTCTGCGGAGGATTg                                     >  4:147895/1‑148 (MQ=255)                                                                                                                  tctTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTa                                   >  7:299203/1‑149 (MQ=255)                                                                                                                    ttGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGAtt                                      <  2:121572/144‑1 (MQ=255)                                                                                                                    ttGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGAtt                                      >  1:121572/1‑144 (MQ=255)                                                                                                                    ttGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATc                                 >  1:102765/1‑149 (MQ=255)                                                                                                                    ttGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATc                                 >  8:245766/1‑149 (MQ=255)                                                                                                                        aGCTCAGTTGGTAGAGCACACCCTTGGTAAGGTGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGAt                              >  1:44509/1‑149 (MQ=255)                                                                                                                         gCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATgg                            <  4:156967/149‑1 (MQ=255)                                                                                                                         gCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATgg                            >  2:136737/1‑149 (MQ=255)                                                                                                                            cAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCt                          >  3:300654/1‑148 (MQ=255)                                                                                                                             aGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTaa                        <  5:41479/149‑1 (MQ=255)                                                                                                                              gTTTGTAGAGCACAACCTTGGTAAGG‑GTGAGGTCATCGGTTAAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAg                       <  5:264998/149‑1 (MQ=255)                                                                                                                                  gTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAATTAGAAATTTTAAAATTTATGCCTTTTTTTTATAAGTGCAAGACTATTTATTATGCGGAGGATTTTATCGATGGCTAAGGa                     >  1:115041/1‑148 (MQ=255)                                                                                                                                  gTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGaa                    >  7:34848/1‑148 (MQ=255)                                                                                                                                  gTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGAAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGaa                    >  6:297883/1‑148 (MQ=255)                                                                                                                                    agagCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCTAGACTATTTCTTCTGCGGAGGAtt                                      <  4:244580/128‑1 (MQ=255)                                                                                                                                    agagCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGAtt                                      >  3:244580/1‑128 (MQ=255)                                                                                                                                       gCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGttt              <  7:162321/149‑1 (MQ=255)                                                                                                                                       gCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGttt              <  8:161047/149‑1 (MQ=255)                                                                                                                                        ctcacCCTTACCAAGG‑GTGTGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCaa                                                             >  4:177203/3‑101 (MQ=255)                                                                                                                                        ctcacCCTTACCAAGG‑GTGTGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCaa                                                             <  3:177203/99‑1 (MQ=255)                                                                                                                                        ctcacCCTTACCAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGttt              >  2:102285/3‑148 (MQ=255)                                                                                                                                         acacCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGTTTGa            <  1:136737/149‑1 (MQ=255)                                                                                                                                               ttGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGTTTGATCGttc      >  2:294637/1‑149 (MQ=255)                                                                                                                                                  gTAAGG‑GTGATGTCATCGGTTCAAGTCAGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGTTTGATCGttccc    <  4:97072/148‑1 (MQ=255)                                                                                                                                                  gTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTAt                                                                     >  4:257458/1‑83 (MQ=255)                                                                                                                                                  gTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTAt                                                                     <  3:257458/83‑1 (MQ=255)                                                                                                                                                   tAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGTTTGATCGttccctt  <  8:299203/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                       CAAGCTCCGCGGGTATAGTTTAGTGGTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGG‑GTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAA‑GGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGTTTGATCGTTCCCTT  >  NC_002947/532471‑532765

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑