| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 53,278 | T→C | 7.4% | G295G (GGT→GGC) | opdT‑I → | tyrosine‑specific outer membrane porin D |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 53,278 | 0 | T | C | 7.4% | 153.9 / 2.0 | 55 | G295G (GGT→GGC) | opdT‑I | tyrosine‑specific outer membrane porin D |
| Reads supporting (aligned to +/- strand): ref base T (26/24); new base C (2/2); total (28/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01 | |||||||||||
GAATCAGTACTACGCCGGCACAAGCGTGGTTTACCCGCTGAGCGACGATCTGGCGCTGATCGGTGGCTTCAACTACTACAAAGCCGTAGATGAAGGTAAGAAGCGCCTGGGTGAATTCGACAACAACATCTGGAGTGCCAAGGTCGGTGTGCGTTACGGTGCCCACACCCTGGCCCTGTCGCATCAGCGCAACAACGGC‑‑GACGACGATTTCGACTACCTGCGTCAGTCGGACTCGATTTTCGTCGACAACTCCATCCAGTACAGCGACTTCAACTCGCCGAAAGAGCGTTCCTGGA > NC_002947/53131‑53426 | gAATCAGTACTACGCCGGCACAAGCGTGGTTTACCCGCTGAGCGACGATCTGGCGCTGATCGGTGGCTTCAACTACTACAAAGCCGTAGATGAAGGTAAGAAGCGCCTGGGTGAATTCGACAACAACATCTGGAGTGCCAAGGTCGgt < 5:356070/148‑1 (MQ=255) ctacGCCGGCACAAGCGTGGTTTACCCGCTGAGCGACGATCTGGCGCTGATCGGTGGCTTCAACTACTACAAAGCCGTAGATGAAGGTAAGAAGCGCCTGGGTGAATTCGACAACAACATCTGGAGTGCCAAGGTCGGTGTGCGTTAc < 2:164154/148‑1 (MQ=255) gCCGGCACAAGCGTGGTTTACACGATGAGCGACGATCTGGCGCTGATCGGTGGCTTCAACTACTACAAAGCCGTAGATGAAGGTAAGAAGCGCCTGGGTGAATTCGACAACAACATCTGGAGTGCCAAGGTCGGTGTGCGTTACGGTGc > 2:87785/1‑149 (MQ=255) ggCACAAGCGTGGTTTACCCGCTGAGCGACGATCTGGCGCTGATCGGTGGCTTCAACTACTACAAAGCCGTAGATGAAGGTAAGAAGCGCCTGGGTGAATTCGACAACAACATCTGGAGTGCCAAGGTCGGTGTGCGTTACGGTGCCca > 4:43516/1‑149 (MQ=255) aGCGTGGTTTACCCGCTGAGCGACGATCTGGCGCTGATCGGTGGCTTCAACTACTACAAAGCCGTAGATGAAGGTAAGAAGCGCCTGGGTGAATTCGACAACAACATCTGGAGTGCCAAGGTCGGTGTGCGTTACGGTGCCCACACCCt > 4:277573/1‑149 (MQ=255) cGTGGTTTACCCGCTGAGCGACGATCTGGCGCTGATCGGTGGCTTCAACTACTACAAAGCCGTAGATGAAGGTAAGAAGCGCCTGGGTGAATTCGACAACAACATCTGGAGTGCCAAGGTCGGTGTGCGTTACGGTGCCCACACCCTgg < 1:43917/149‑1 (MQ=255) gTGGTTTACCCGCTGAGCGACGATCTGGCGCTGATCGGTGGCTTCAACTACTACAAAGCCGTAGATGAAGGTAAGAAGCGCCTGGGTGAATTCGACAACAACATCTGGAGTGCCAAGGTCGGTGTGCGTTACGGTGCCCACACCCTGGc < 4:292660/149‑1 (MQ=255) tGAGCGACGATCTGGCGCTGATCGGTGGCTTCAACTACTACAAAGCCGTAGATGAAGGTAAGAAGCGCCTGGGTGAATTCGACAACAACATCTGGAGTGCCAAGGTCGGTGTGCGTTACGGTGCCCACACCCTGGCCCTGTCGCATCAg > 6:173005/1‑149 (MQ=255) gaTCTGGCGCTGATCGGTGGCTTCAACTACTACAAAGCCGTAGATGAAGGTAAGAAGCGCCTGGGTGAATTCGACAACAACATCTGGAGTGCCAAGGTCGGTGTGCGTTACGGTGCCCACACCCTGGCCCTGTCGCATCAGCGcaacaa > 7:208237/1‑149 (MQ=255) gcgcTGATCGGTGGCTTCAACTACTACAAAGCCGTAGATGAAGGTAAGAAGCGCCTGGGTGAATTCGACAACAACATCTGGAGTGCCAAGGTCGGTGTGCGTTACGGTGCCCACACCCTGGCCCTGTCGCATCAGCGCAACAACg < 8:40401/145‑1 (MQ=255) gcgcTGATCGGTGGCTTCAACTACTACAAAGCCGTAGATGAAGGTAAGAAGCGCCTGGGTGAATTCGACAACAACATCTGGAGTGCCAAGGTCGGTGTGCGTTACGGTGCCCACACCCTGGCCCTGTCGCATCAGCGCAACAACGGc‑‑ga > 3:280923/1‑149 (MQ=255) cTGATCGGTGGCTTCAACTACTACAAAGCCGTAGATGAAGGTAAGAAGCGCCTGGGTGAATTCGACAACAACATCTGGAGTGCCAAGGTCGGTGTGCGTTACGGTGCCCACACCCTGGCCCTGTCGCATCAGCGCAACAACGGc‑‑gacga < 3:43516/149‑1 (MQ=255) gTGGCTTCAACTACTACAACGCCGTAGATGAAGGTAAGAAGCGCCTGGGTGAATTCGACAACAACATCTGGAGTGCCAAGGTCGGTGTGCGTTACGGTGCCCACACCCTGGCCCTGTCGCATCAGCGCAACAACGGC‑‑GACGACGATTTc < 1:112662/149‑1 (MQ=255) gTGGCTTCAACTACTACAAAGCCGTAGATGAAGGTAAGACGCGCCTGGGTGAATTCGACAACAACATCTGGNGTGCCAAGGTCGGTGTGCGTTACGGTGCCCACACCCTGGCCCTGTCGCATCAGCGCAACAACGGC‑‑GACGACGATTTc < 1:116999/149‑1 (MQ=255) gTGGCTTCAACTACTACAAAGCCGTAGATGAAGGTAAGAAGCGCCTGGGTGAATTCGACAACAACATCTGGAGTGCCAAGGTCGGTGTGCGTTACGGTGCCCACACCCTGGCCCTGTCGCATCAGCGCAACAACGGC‑‑GACGACGATTTc < 8:72699/149‑1 (MQ=255) gTGGCTTCAACTACTACAAAGCCGTAGATGAAGGTAAGAAGCGCCTGGGTGAATTCGACAACAACATCTGGAGTGCCAAGGTCGGTGTGCGTTACGGTGCCCACACCCTGGCCCTGTCGCATCAGCGCAACAACGGC‑‑GACGACGATTTc < 5:128017/149‑1 (MQ=255) gTGGCTTCAACTACTACAAAGCCGTAGATGAAGGTAAGAAGCGCCTGGGTGAATTCGACAACAACATCTGGAGTGCCAAGGTCGGCGTGCGTTACGGTGCCCACACCCTGGCCCTGTCGCATCAGCGCAACAACGGC‑‑GACGACGATTTc > 2:166129/1‑149 (MQ=255) gCTTCTACTAGTACAAAGCCTTAGTTGAAGGTAAGAAGCGACTGGGTGAATTCGACTACAACATCTGTAGTGCGAAGTTCGGGGTGCTTTACGTTTCCCACACCCTGGCCCTGTCGCATCAGCTCAACAACGGC‑‑GACGACTATTTCGag < 8:35379/149‑2 (MQ=255) cAACTACTACAAAGCCGTAGATGAAGGTAAGAAGCGCCTGGGTGAATTCGACAACAACATCTGGAGTGCCAAGGTCGGTGTGCGTTACGGTGCCCACACCCTGGCCCTGTCGCATCAGCGCAACAACGGC‑‑GACGACGATTTCGACTAc < 1:87785/148‑1 (MQ=255) tacAAAGCCGTAGATGAAGGTAAGAAGCGCCTGGGTGAATTCGACAACAACATCTGGAGTGCCAAGGTCGGTGTGCGTTACGGTGCCCACACCCTGGCCCTGTCGCATCAGCGCAACAACGGC‑‑GACGACGATTTCGACTACCTGCGt > 4:103339/1‑147 (MQ=255) tacAAAGCCGTAGATGAAGGTAAGAAGCGCCTGGGTGAATTCGACAACAACATCTGGAGTGCCAAGGTCGGTGTGCGTTACGGTGCCCACACCCTGGCCCTGTCGCATCAGCGCAACAACGGC‑‑GACGAAGATTTCGACTACCTGCGTCa > 5:221121/1‑149 (MQ=255) tacAAAGCCGTAGATGAAGGTAAGAAGCGCCTGGGTGAATTCGACAACAACATCTGGAGTGCCAAGGTCGGCGTGCGTTACGGTGCCCACACCCTGGCCCTGTCGCATCAGCGCAACAACGGC‑‑GACGACGATTTCGACTACCTGCGt > 7:94933/1‑147 (MQ=255) cAAAGCCGTAGATGAAGGTAAGAAGCGCCTGGGTGAATTCGACAACAACATCTGGAGTGCCAAGGTCGGTGTGCGTTACGGTGCCCACACCCTGGCCCTGTCGCATCAGCGCAACAACGGC‑‑GACGACGATTTCGACTACCTGCGTCAGt > 6:118267/1‑149 (MQ=255) cAAAGCCGTAGATGAAGGTAAGAAGCGCCTGGGTGAATTCGACAACAACATCTGGAGTGCCAAGGTCGGTGTGCGTTACGCTGCCCACACCCTGGCCCTGTCGCATCATCGCAACAACGGC‑‑GACGACGATTTCGACTACCTGCGTCAGt > 5:10445/1‑149 (MQ=255) aaaGCCGTAGATGAAGGTAAGAAGCGCCTGGGTGAATTCGACAACAACATCTGGAGTGCCAAGGTCGGTGTGCGTTACGGTGCCCACACCCTGGCCCTGTCGCATCAGCGCAACAACGGC‑‑GACGACGATTTCGACTACCTGCGTCAGTc > 7:211024/1‑149 (MQ=255) gCCGTAGATGAAGGTAAGAAGCGCCTGGGTGAATTCGACAACAACATCTGGAGTGCCAAGGTCGGTGTGCGTTACGGTGCCCACACCCTGGCCCTGTCGCATCAGCGCAACAACGGC‑‑GACGACGATTTCGACTACCTGCGTCAGTCGGa < 4:280923/149‑1 (MQ=255) cGTAGATGAAGGTAAGAAGCGCCTGGGTGAATTCGACAACAACATCTGGAGTGCCAAGGTCGGTGTGCGTTACGGTGCCCACACCCTGGCCCTGTCGCATCAGCGCAACAACGGC‑‑GACGACGATTTCGACTACCTGCGTCAGTCGGACt < 8:208237/149‑1 (MQ=255) gTAGATGAAGGTAAGAAGCGCCTGGGTGAATTCGACAACAACATCTGGAGTGCCAAGGTCGGTGTGCGTTACGGTGCCCACACCCTGGCCCTGTCGCATCAGCGCAACAACGGC‑‑GACGACGATTTCGACTACCTGCGTCAGTCGGACTc > 8:91128/1‑149 (MQ=255) tGAAGGTAAGAAGCGCCTGGGTGAATTCGACAACAACATCTGGAGTGCCAAGGTCGGTGTGCGTTACGGTGCCCACACCCTGGCCCTGTCGCATCAGCGCAACAACGGC‑‑GACGACGATTTCGACTACCTGCGTCAGTCGGACTCGAttt < 7:91128/149‑1 (MQ=255) tGAAGGTAAGAAGCGCCTGGGTGAATTCGACAACAACATCTGGAGTGCCAAGGTCGGTGTGCGTTACGGTGCCCACACCCTGGCCCTGTCGCATCAGCGCAACAACGGC‑‑GACGACGATTTCGACTACCTGCGTCAGTCGGACTCGAttt < 5:173005/149‑1 (MQ=255) aGGTAAGAAGCGCCTGGGTGAATTCGACAACAACATCTGGAGTGCCAAGGTCGGTGTGCGTTACg < 6:350858/65‑1 (MQ=255) aGGTAAGAAGCGCCTGGGTGAATTCGACAACAACATCTGGAGTGCCAAGGTCGGTGTGCGTTACg > 5:350858/1‑65 (MQ=255) aGGTAAGAAGCGCCTGGGTGAATTCGACAACAACATCTGGAGTGCCAAGGTCGGCGTGCGTTACGGTGCCCACACCCTGGCCCTGTCGCATCAGCGCAACAACGGC‑‑GACGACGATTTCGACTACCTGCGTCAGTCGGACTCGATTTtc < 8:94933/148‑1 (MQ=255) ggTAAGAAGCGCCTGGGTGAATTCGACAACAACATCTGGAGTGCCAAGGTCGGTGTGCGTTACGGTGCCCACACCCTGGCCCTGTCGCATCAGCGCAACAACGGC‑‑GACGACGATTTCGACTACCTGCGTCAGTCGGACTCGATTTtcgt > 6:159674/1‑149 (MQ=255) gTAAGAAGCGCCTGGGTGAATTCGACAACAACATCTGGAGTGCCAAGGTCGGTGTGCGTTACGGTGCCCACACCCTGGCCCTGTCGCATCAGCGCAACAACGGC‑‑GACGACGATTTCGACTACCTGCGTCAGTCGGACTCGATTTtcgtc > 5:47817/1‑149 (MQ=255) tAAGAAGCGCCTGGGTGAATTCGACAACAACATCTGGAGTGCCAAGGTCGGTGTGCGTTACGGTGCCCACACCCTGGCCCTGTCGCATCAGCGCAACAACGGC‑‑GACGACGATTTCGACTa > 6:144471/1‑120 (MQ=255) aagaagCGCCTGGGTGAATTCGACAACAACATCTGGAGTGCCAAGGTCGGTGTGAGTTAAGGTGCCCACACCCTGGCCCTGTCGCATCAGCGCAACAACGGC‑‑GACGACGATTTCGACTACCTGCGTCAGTCGGACTCGATTTtcgtcgc > 5:350729/1‑148 (MQ=255) gggTGAATTCGACAACAACATCTGGAGTGCCAAGGTCGGTGTGCGTTACGGTGCCCACACCCTGGCCCTGTCGCATCAGCGCAACAACGGC‑‑GACGACGATTTCGACTACCTGCGTCAGTCg < 4:343422/121‑1 (MQ=255) gggTGAATTCGACAACAACATATGGAGTGCCAAGGTCGGTGTGCGTTACGGTGCCCACACCCTGCCCCTGTCGCATAAGCGCAACAACGGC‑‑GACGACGATTTCGACTACCTGCGTCAGTCGGACTCGATTTTAGTCGACAACtccatc > 5:467/1‑148 (MQ=255) ggTGAATTCGACAACAACATCTGGAGTGCCAAGGTCGGTGTGCGTTACGGTGCCCACACCCTGGCCCTGTCGCATCAGCGCAACAACGGC‑‑GACGACGATTTCGACTACCTGCGTCAGTCGGACTCGATTTTCGTCGACAACtccatc < 3:277573/147‑1 (MQ=255) aaTTCGACAACAACATCTGGAGTGCCAAGGTCGGTGTGCGTTACGGTGCCCACACCCTGGCCCTGTc < 6:123840/67‑1 (MQ=255) aTTCGACAACAACATCTGGAGTGCCAAGGTCGGTGTGCGTTACGGTGCCCACACCCTGGCCCTGTCGCATCAGCGCAACAACGGc‑‑gacgacg < 1:129544/92‑1 (MQ=255) aTTCGACAACAACATCTGGAGTGCCAAGGTCGGTGTGCGTTACGGTGCCCACACCCTGGCCCTGTCGCATCAGCGCAACAACGGc‑‑gacgacg > 2:129544/1‑92 (MQ=255) tCGACAACAACATCTGGAGTGCCAAGGTCGGTGTGCGTTACGGTGCCCACACCCTGGCCCTGTCGCATCAGCGCAACAACGGC‑‑GACGACGATTTCGACTACCTGCGTCAGTCGGACTCGATTTtc > 6:344231/1‑125 (MQ=255) tCGACAACAACATCTGGAGTGCCAAGGTCGGTGTGCGTTACGGTGCCCACACCCTGGCCCTGTCGCATCAGCGCAACAACGGC‑‑GACGACGATTTCGACTACCTGCGTCAGTCGGACTCGATTTtc < 5:344231/125‑1 (MQ=255) tCGACAACAACATCTGGAGTGCCAAGGTCGGTGTGCGTTACGGTGCCCACACCCTGGCCCTGTCGCATCAGCGCAACAACGGC‑‑GACGACGATTTCGACTACCTGCGTCAGTCGGACTCGATTTTCGTCGACAACTCCATCCAGt > 4:85066/1‑144 (MQ=255) tCGACAACAACATCTGGAGTGCCAAGGTCGGTGTGCGTTACGGTGCCCACACCCTGGCCCTGTCGCATCAGCGCAACAACGGC‑‑GACGACGATTTCGACTACCTGCGTCAGTCGGACTCGATTTTCGTCGACAACTCCATCCAGt < 3:85066/144‑1 (MQ=255) acaacaacaTCTGGAGTGCCAAGGTCGGTGTGCGTTACGGTGCCCACACCCTGGCCCTGTCGCATCAGCGCAACAACGGC‑‑GACGACGATTTCGACTACCTGCGTCAGTCGGACTCGATTTTCGTCGACAACTCCATCCAGTACAGCGAc > 7:20554/1‑149 (MQ=255) acaacaacaTCTGGAGTGCCAAGGTCGGTGTGCGTTACGGGGCCCACACCCTGGCCCTGTCGCATCAGCGCAACAACGGC‑‑GACGACGATTTCGACTACCTGCGTCAGTCGGACTCGATTTTCGTCGACAACTCCATCCAGTACAGCGAc < 6:50269/149‑1 (MQ=255) aacaacaTCTGGAGTGCCAAGGTCGGTGTGCGTTACGGTGCCCACACCCTGGCCCTGTCGCATCAGCGCAACAACGGC‑‑GACGACGATTTCGACTACCTGCGTCAGTCGGACTCGATTTTCGTCGACAACTCCATCCAGTACAGCGACtt > 2:94724/1‑149 (MQ=255) cTGGAGTGCCAAGGTCGGTGTGCGTTACGGTGCCCACACCCTGGCCCTGTCGCATCAGCGCAACAACGGCCAGGCGACGATTTCGACTACATGCGTCAGTCGGCCTCGATTTTCGTCGCCAAGTCAAGCAAGTACAGCGAGTTCACCTc > 1:115983/1‑149 (MQ=255) cTGGAGTGCCAAGGTCGGTGTGCGTTACGGTGCCCACACCCTGGCCCTGTCGCATCAGCGCAACAACGGC‑‑GACGACGATTTCGACTACCTGCGTCAGTCGGACTCGATTTTCGTCGACAACTCCATCCAGTACAGCGACTTCAACTCGc > 6:370055/1‑149 (MQ=255) gAGTGCCAAGGTCGGTGTGCGTTACGGTGCCCACACCCTGGCCCTGTCGCATCAGCGCAACAACGGC‑‑GACGACGATTTCGACTACCTGCGTCAGTCGGACTCGATTTTCGTCGACAACTCCATCCAGTACAGCGACTTCAACTCGc < 6:47817/146‑1 (MQ=255) ccAAGGTCGGTGTGCGTTACGGTGCCCACACCCTGGCCCTGTCGCATCAGCGCAACAACGGC‑‑GACGACGATTTCGACTACCTGCGTCAGTCGGACTCGATTTTCGTCGACAACTCCATCCAGTACAGCGACTTCAACTCGCCGAAaga < 1:94724/148‑1 (MQ=255) cAAGGTCGGCGTGCGTTACGGTGCCCACACCCTGGCCCTGTCGCATCAGCGCAACAACGGC‑‑GACGACGATTTCGACTACCTGCGTCAGTCGGACTCGATTTTCGTCGACAACTCCATCCAGTACAGCGACTTCAACTCGCCGAAAGAGc < 1:166129/149‑1 (MQ=255) ggTCGGTGTGCGTTACGGTGCCCACACCCTGGCCCTGTCGCATCAGCGCAACAACGGC‑‑GACGACGATTTCGACTACCTGCGTCAGTCGGACTCGATTTTCGTCGACAACTCCATCCAGTACAGCGACTTCAACTCGCCGAAAGAGAGt > 4:49691/1‑148 (MQ=255) tgtgCGTTACGGTGCCCTCACCCTGGCCCTGTCGCATCAGCGCAACAACGGC‑‑GACGACGATTTCTACTACCTGCGTCAGTCGGACTCGATTTTCGTCGACAACTCCATCCAGTACAGCGACTTCAACTCGCCGAAAGAGCGTTCCTGGa < 6:10445/149‑1 (MQ=255) tgtgCGTTACGGTGCCCACACCCTGGCCCTGTCGCATCAGCGCAACAACGGC‑‑GACGACGATTTCGACTACCTGCGTCAGTCGGACTCGATTTTCGTCGACAACTCCATCCAGTACAGCGACTTCAACTCGCCGAAAGAGCGTTCCTGGa < 1:61732/149‑1 (MQ=255) | GAATCAGTACTACGCCGGCACAAGCGTGGTTTACCCGCTGAGCGACGATCTGGCGCTGATCGGTGGCTTCAACTACTACAAAGCCGTAGATGAAGGTAAGAAGCGCCTGGGTGAATTCGACAACAACATCTGGAGTGCCAAGGTCGGTGTGCGTTACGGTGCCCACACCCTGGCCCTGTCGCATCAGCGCAACAACGGC‑‑GACGACGATTTCGACTACCTGCGTCAGTCGGACTCGATTTTCGTCGACAACTCCATCCAGTACAGCGACTTCAACTCGCCGAAAGAGCGTTCCTGGA > NC_002947/53131‑53426 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |