Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	642,527	C→G	6.8%	A154P (GCG→CCG)	acoC ←	acetoin cleaving system dihydrolipoyllysine‑residue acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	642,527	0	C	G	6.8%	187.9 / 4.1	59	A154P (GCG→CCG)	acoC	acetoin cleaving system dihydrolipoyllysine‑residue acetyltransferase
Reads supporting (aligned to +/- strand):  ref base C (23/32);  new base G (2/2);  total (25/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.73e-01

GCCTTGGCGATGTCCAGGTGGTCGAGCAGGGCGAGCACGGTTTCGCTCAACTCATCCAGGTCGCCCCGCTGCAGGGCCTTGGCCGACTCGCCGTGGCCCGGCAGGTCGAGGGCGATGACCCGCCGCTCGGCAGCC‑AGCGCCGGGTGGTTGAACAGCCAGTTGTTGAGGTCACCACCAAACCCGTGCACCAGCACCAGCGGTGTACCGCCTTCACCCAGCTCGAACCAGCGCAGCAGGCGCCCGCCTACTTCCGCCTTCTGAGGGGCCGGCCCCTG  >  NC_002947/642388‑642662                                                                                                                                             |                                                                                                                                        gCCTTGGCGATGTCCAGGTGGTCGAGCAGGGCGAGCACGGTTTCGCTCAACTCATCCAGGTCGCCCCGCTGCAGGGCCTTGGCCGACTCGCCGTGGCCCGGCAGGTCGAGGGCGATGACCCGCCGCTCGGCAGCC‑AGCGCCGGGTGGtt                                                                                                                                >  3:78913/1‑149 (MQ=255)  ccTTGGCGATGTCCAGGTGGTCGAGCAGGGCGAGCACGGTTTCGCTCAACTCATCCAGGTCGCCCCGCTGCAGGGCCTTGGCCGACTCGCCGTGGCCCGGCAGGTCGAGGGCGATGACCCGGCGCT‑GGCTGCCGAGCGGCGggtcatcg                                                                                                                               <  3:90181/149‑6 (MQ=255)       gCGATGTCCAGGTGGTCGAGCAGGGCGAGCACGGTTTCGCTCAACTCATCCAGGTCGCCCCGCTGCAGGGCCTTGGCCGACTCGCCGTGGCCCGGCAGGTCGAGGGCGATGACCCGCCGCTCGGCAGCC‑AGCGCCGGGTGGTTGAACa                                                                                                                           <  7:295093/148‑1 (MQ=255)       gCGATGTCCAGGTGGTCGAGCAGGGCGAGCACGGTTTCGCTCAACTCATCCAGGTCGCCCCGCTGCAGGGCCTTGGCCGACTCGCCGTGGCCCGGCAGGTCGAGGGCGATGACCCGCCGCTCGGCAGCC‑AGCGCCGGGTGGTTGAACa                                                                                                                           <  7:283648/148‑1 (MQ=255)        cGATGTCCAGGTGGTCGAGCAGGGCGAGCACGGTTTCGCTCAACTCATCCAGGTCGCCCCGCTGCAGGGCCTTGGCCGACTCGCCGTGGCCCGGCAGGTCGAGGGCGATGACCCGCCGCTCGGCAGCC‑AGCGCCGGGTGGTTGAACAGc                                                                                                                         <  4:63408/149‑1 (MQ=255)             tCCAGGTGGTCGAGCAGGGCGAGCACGGTTTCGCTCAACTCATCCAGGTCGCCCCGCTGCAGGGCCTTGGCCGACTCGCCGTGGCCCGGCAGGTCGAGGGCGATGACCCGCCGCTCGGCAGCC‑AGCGCCGGGTGGTTGAACAGCCAgtt                                                                                                                    <  2:23966/149‑1 (MQ=255)               cAGGTGGTCGAGCAGGGCGAGCACGGTTTCGCTCAACTCATCCAGGTCGCCCCGCTGCAGGGCCTTGGCCGACTCGCCGTGGCCCGGCAGGTCGAGGGCGATGACCCGCCGCTCGGCAGCC‑AGCGCCGGGTGGTTGAACAGCCAgttgt                                                                                                                  >  8:343178/1‑149 (MQ=255)                aGGTGGTCGAGCAGGGCGAGCACGGTTTCGCTCAACTCATCCAGGTCGCCCCGCTGCAGGGCCTTGGCCGACTCGCCGTGGCCCGGCAGGTCGAGGGCGATGACCCGCCGCTCGGCAGCC‑AGCGCCGGGTGGTTGAACAGCCAgttgtt                                                                                                                 <  4:12891/149‑1 (MQ=255)                aGGTGGTCGAGCAGGGCGAGCACGGTTTCGCTCAACTCATCCAGGTCGCCCCGCTGCAGGGCCTTGGCCGACTCGCCGTGGCCCGGCAGGTCGAGGGCGATGACCCGCCGCTCGGCAGCC‑AGCGCCGGGTGGTTGAACAGCCAgttgtt                                                                                                                 <  2:113567/149‑1 (MQ=255)                   tggtCGAGCAGGGCGAGCACGGTTTCGCTCAACTCATCCAGGTCGCCCCGCTGCAGGGCCTTGGCCGACTCGCCGTGGCCCGGCAGGTCGAGGGCGATGACCCGCCGCTCGGCAGCC‑AGCGCCGGGTGGTTGAACAGCCAGTTGTTGAg                                                                                                              <  5:368403/149‑1 (MQ=255)                    ggtCGAGCAGGGCGAGCACGGTTTCGCTCAACTCATCCAGGTCGCCCCGCTGCAGGGCCTTGGCCGACTCGCCGTGGCCCGGCAGGTCGAGGGCGATGACCCGCCGCTCGGCAGCC‑AGCGCCGGGTGGTTGAACAGCCAGTTGTTGAgg                                                                                                             <  5:324219/149‑1 (MQ=255)                         aGCAGGGCGAGCACGGTTTCGCTCAACTCATCCAGGTCGCCCCGCTGCAGGGCCTTGGCCGACTCGCCGTGGCCCGGCAGGTCGAGGGCGATGACCCGCCGCTCGGCAGCC‑AGCGCCGGGTGGTTGAACAGCCAGTTGTTGAGGTcacc                                                                                                        <  3:144374/149‑1 (MQ=255)                           cAGGGCGAGCACGGTTTCGCTCAACTCATCCAGGTCGCCCCGCTGCAGGGCCTTGGCCGACTCGCCGTGGCCCGGCAGGTCGAGGGCGATGACCCGCCGCTCGGCAGCC‑AGCGCCGGGTGGTTGAACAGCCAGTTGTTGAGGTcaccac                                                                                                      <  3:72039/149‑1 (MQ=255)                                   gCACGGTTTCGCTCAACTCATCCAGGTCGCCCCGCTGCAGGGCCTTGGCCGACTCGCCGTGGCCCGGCAGGTCGAGGGCGATGACCCGCCGCTCGGCAGCC‑AGCGCCGGGTGGTTGAACAGCCAGTTGTTGa                                                                                                               <  1:237126/132‑1 (MQ=255)                                   gCACGGTTTCGCTCAACTCATCCAGGTCGCCCCGCTGCAGGGCCTTGGCCGACTCGCCGTGGCCCGGCAGGTCGAGGGCGATGACCCGCCGCTCGGCAGCC‑AGCGCCGGGTGGTTGAACAGCCAGTTGTTGa                                                                                                               >  2:237126/1‑132 (MQ=255)                                         tttCGCTCAACTCATCCAGGTCGCCCCGCTGCAGGGCCTTGGCCGACTCGCCGTGGCCCGGCAGGTCGAGGGCGATGACCCGCCGCTCGGCAGCC‑AGCGCCGGGTGGTTGAACAGCCAGTTGTTGAGGTCACCACCAAACCCGTgcacc                                                                                        <  7:343178/149‑1 (MQ=255)                                           tCGCTCAACTCATCCAGGTCGCCCCGCTGCAGGGCCTTGGCCGACTCGCCGTGGCCCGGCAGGTCGAGGGCGATGACCCGCCGCTCGGCAGCC‑AGCGCCGGGTGGTTGAACAGCCAGTTGTTGAGGTCACCACCAAACCCGTgcacca                                                                                       <  8:22090/148‑1 (MQ=255)                                                 aaCTCATCCAGGTCGCCCCGCTGCAGGGCCTTGGCCGACTCGCCGTGGCCCGGCAGGTCGAGGGCGATGACCCGCCGCTCGGCAGCC‑AGCGCCGGGTGGTTGAACAGCCAGTTGTTGAGGTCACCACCAAACCCGt                                                                                             <  2:311177/136‑1 (MQ=255)                                                 aaCTCATCCAGGTCGCCCCGCTGCAGGGCCTTGGCCGACTCGCCGTGGCCCGGCAGGTCGAGGGCGATGACCCGCCGCTCGGCAGCC‑AGCGCCGGGTGGTTGAACAGCCAGTTGTTGAGGTCACCACCAAACCCGt                                                                                             >  1:311177/1‑136 (MQ=255)                                                   cTCATCCAGGTCGCCCCGCTGCAGGGCCTTGGCCGACTCGCCGTGGCCCGGCAGGTCGAGGGCGATGACCCGCCGCTCGGTAGCC‑AGCGCCGGGTGGTTGAACAGCCAGTTGTTGAGGTCACCACCAAACCCGTGCACCAGCACCAGCg                                                                              <  8:322998/149‑1 (MQ=255)                                                   cTCATCCAGGTCGCCCCGCTGCAGGGCCTTGGCCGACTCGCCGTGGCCCGGCAGGTCGAGGGCGATGACCCGCCGCTCGGCAGCC‑AGCGCCGGGTGGTTGAACAGCCAGTTGTTGAGGTCACCACCAAACCCGTGCACCAGCACCAGCg                                                                              >  3:214277/1‑149 (MQ=255)                                                    tcatcaAGGTCGCCCCGCTGCAGGGCCTTGGCCGACTCGCCGTGGCCCGGCAGGTCGAGGGCGATGACCCGCCGCTCGGCAGCC‑AGCGCCGGGTGGTTGAACAGCCAGTTGTTGAGGTCACCACCAAACCCGTGCACCAGCACCAGCg                                                                              >  7:120052/1‑148 (MQ=255)                                                      aTCCAGGTCGCCCCGCTGCAGGGCCTTGGCCGACTCGCCGTGGCCCGGCAGGTCGAGGGCGATGACCCGCCGCTCGGCAGCC‑AGCGCCGGGTGGTTGAACAGCCAGTTGTTGAGGTCACCACCAAACCCGTGCACCAGCACCAGCg                                                                              <  8:120052/146‑1 (MQ=255)                                                      aTCCAGGTCGCCCCGCTGCAGGGCCTTGGCCGACTCGCCGTGGCCCGGCAGGTCGAGGGCGATGACCCGCCGCTCGGCAGCC‑AGCGCCGGGTGGTTGAACAGCCAGTTGTTGAGGTCACCACCAAACCCGTGCACCAGCACCAGCGgtg                                                                           >  3:265427/1‑149 (MQ=255)                                                            gTCGCCCCGCTGCAGGGCCTTGGCCGACTCGCCGTGGCCCGGCAGGTCGAGGGCGATGACCCGCCGCTCGGCAGCC‑AGCGCCGGGTGGTTGAACAGc                                                                                                                         >  4:139303/1‑97 (MQ=255)                                                            gTCGCCCCGCTGCAGGGCCTTGGCCGACTCGCCGTGGCCCGGCAGGTCGAGGGCGATGACCCGCCGCTCGGCAGCC‑AGCGCCGGGTGGTTGAACAGc                                                                                                                         <  3:139303/97‑1 (MQ=255)                                                                   cGCTGCAGGGCCTTGGCCGACTCGCCGTGGCCCGGCAGGTCGAGGGCGATGACCCGCCGCTCGGCAGCC‑AGCGCCGGGTGGTTGAACAGCCAGTTGTTGAGGTCACCACCAAACCCGTGCACCAGCACCAGCGGTGTACCGCCTTCAcc                                                              <  6:149545/149‑1 (MQ=255)                                                                        cAGGGCCTTGGCCGACTCGCCGTGGCCCGGCAGGTCGAGGGCGATGACCCGCCGCTCGGCAGCC‑AGCGCCGGGTGGTTGAACAGCCAGTTGTTGAGGTCACCACCAAACCCGTGCACCAGCACCAGCGGTGTACCGCCTTCACCCAGCt                                                         <  4:78913/149‑1 (MQ=255)                                                                        cAGGGCCTTGGCCGACTCGCCGTGGCCCGGCAGGTCGAGGGCGATGACCCGCCGCTCGGCAGCC‑AGCGCCGGGTGGTTGAACAGCCAGTTGTTGAGGTCACCACCAAACCCGTGCACCAGCACCAGCGGTGTACCGCCTTCACCCAGCt                                                         >  6:278087/1‑149 (MQ=255)                                                                            gCCTTGGCCGACTCGCCGTGGCCCGGCAGGTCGAGGGCGATGACCCGCCGCTCGGCAGCC‑AGCGCCGGGTGGTTGAACAGCCAGTTGTTGAGGTCACCACCAAACCCGTGCACCAGCACCAGCGGTGTACCGCCTTCACCCAGCTCGaa                                                     >  8:44058/1‑149 (MQ=255)                                                                             ccTTGGCCGACTCGCCGTGGCCCGGCAGGTCGAGGGCGATGACCCGCCGCTCGGCAGCC‑AGCGCCGGGTGGTTGAACAGCCAGTTGTTGAGGTCACCACCAAACCCGTGCACCAGCACCAGCGGTGTACCGCCTTCAccc                                                             >  3:97389/1‑140 (MQ=255)                                                                             ccTTGGCCGACTCGCCGTGGCCCGGCAGGTCGAGGGCGATGACCCGCCGCTCGGCAGCC‑AGCGCCGGGTGGTTGAACAGCCAGTTGTTGAGGTCACCACCAAACCCGTGCACCAGCACCAGCGGTGTACCGCCTTCAccc                                                             <  4:97389/140‑1 (MQ=255)                                                                             ccTTGGCCGACTCGCCGTGGCCCGGCAGGTCGAGGGCGATGACCCGCCGCTCGGCAGCC‑AGCGCCGGGTGGTTGAACAGCCAGTTGTTGAGGTCACCACCAAACCCGTGCACCAGCACCAGCGGTGTACCGCCTTCACCCAGCTCGAAc                                                    <  4:214277/149‑1 (MQ=255)                                                                             ccTTGGCCGACTCGCCGTGGCCCGGCAGGTCGAGGGCGATGACCCGCCGCTCGGCAGCC‑AGCGCCGGGTGGTTGAACAGCCAGTTGTTGAGGTCACCACCAAACCCGTGCACCAGCACCAGCGGTGTACCGCCTTCACCCAGCTCGAAc                                                    <  3:122081/149‑1 (MQ=255)                                                                             ccTTGGCCGACTCGCCGTGGCCCGCCAGGTCGAGGGCGAAGACCCGCCGCTCGGAAGCC‑AGAGCCGGGTGGTTGAACAGCCAGTTGTTGAGGTCACCACCAAACCCGTGCACCAGCACCAGCGGAGTACCGCCTTCACCCAGCTCGAAc                                                    >  1:141464/1‑149 (MQ=255)                                                                              cTTGGCCGACTCGCCGTGGCCCGGCAGGTCGAGGGCGATGACCCGCCGCTCGGCAGCC‑AGCGCCGGGTGGTTGAACAGCCAGTTGTTGAGGTCACCACCAAACCCGTgcaccag                                                                                      <  2:53781/114‑1 (MQ=255)                                                                              cTTGGCCGACTCGCCGTGGCCCGGCAGGTCGAGGGCGATGACCCGCCGCTCGGCAGCC‑AGCGCCGGGTGGTTGAACAGCCAGTTGTTGAGGTCACCACCAAACCCGTgcaccag                                                                                      >  1:53781/1‑114 (MQ=255)                                                                                    cGACTCGCCGTGGCCCGGCAGGTCGAGGGCGATGACCCGGCGCT‑GGCTGCCGAGCGGCggg                                                                                                                                     >  2:319627/1‑61 (MQ=18)                                                                                    cGACTCGCCGTGGCCCGGCAGGTCGAGGGCGATGACCCGGCGCT‑GGCTGCCGAGCGGCggg                                                                                                                                     <  1:319627/61‑1 (MQ=18)                                                                                                  ccGGCAGGTCGAGGGCGATGACCCGCCGCTCGGCAGCC‑AGCGCCGGGTGGTTGAACAGCCAGTTGTTGAGGTCACCACCAAACCCGTGCACCAGCACCAGCGGTGTACCGCCTTCACCCAGCTCGAACCAGCGCAGCAGGcgcccgcc                                >  3:288635/1‑148 (MQ=255)                                                                                                   cGGCAGGTCGAGGGCGATGACCCGCCGCTCGGCAGCC‑AGCGCCGGGTGGTTGAACAGCCAGTTGTTGAGGTCACCACCAAACCCGTGCACCAGCACCAGCGGTGTACCGCCTTCACCCAGCTCGAACCAGCGCAGCAGGCGCCCGCCTa                              >  6:114996/1‑149 (MQ=255)                                                                                                   cGGCAGGTCGAGGGCGATGACCCGCCGCTCGGCAGCC‑AGCGCCGGGTGGTTGAACAGCCAGTTGTTGAGGTCACCACCAAACACGTGCACCAGCACCAGCGGTGTACCGCCTTCACCCAGCTCGAACCAGCGCAGCAGGCGCCCGCCTa                              >  4:365141/1‑149 (MQ=255)                                                                                                       aGGTCGAGGGCGATGACCCGCCGCTCGGCAGCC‑AGCGCCGGGTGGTTGAACAGCCAGTTGTTGAGGTCACCACCAAACCCGTGCACCAGCACCAGCGGTGTACCGCCTTCACCCAGCTCGAACCAGCGCAGCAGGCGCCCGCCTACTTc                          >  4:287379/1‑149 (MQ=255)                                                                                                             aGGGCGATGACCCGCCGCTCGGCAGCC‑AGCGCCGGGTGGTTGAACAGCCAGTTGTTGAGGTCACCACCAAACCCGTGCACCAGCACCAGCGGTGTACCGCCTTCACCCAGCTCGAACCAGCGCAGCAGGCGCCCGCCTACTTCCGCCtt                    <  3:287379/149‑1 (MQ=255)                                                                                                              gggCGATGACCCGCCGCTCGGCAGCC‑AGCGCCGGGTGGTTGAACAGCCAGTTGTTGAGGTCACCACCAAACCCGTGCACCAGCACCAGCGGTGTACCGCCTTCACCCAGCTCGAACCAGCGCAGCAGGCGCCCGCCTACTTCCGCCTTc                   <  6:319161/149‑1 (MQ=255)                                                                                                                tcaaccaCCCGGCGCT‑GGCTGCCGAGCGGCGGGTGGTTGAACAGCCAGTTGTTGAGGTCACCACCAAACCCGTGCACCAGCACCAGCGGTGTACCGCCTTCACCCAGCTCGAACCAGCGCAGCAGGCGCCCGCCTACTTCCGCCTTCt                  >  5:134791/7‑148 (MQ=255)                                                                                                                  gATGACCCGCCGCTCGGCAGCC‑AGCGCCGGGTGGTTGAACAGCCAGTTGTTGAGGTCACCACCAAACCCGTGCACCAGCACCAGCGGTGTACCGCCTTCACCCAGCTCGAACCAGCGCAGCAGGCGCCCGCCTACTTCCGCCTTCTGAg               >  3:2767/1‑149 (MQ=255)                                                                                                                  gATGACCCGCCGCTCGGCAGCC‑AGCGCCGGGTGGTTGAACAGCCAGTTGTTGAGGTCACCACCAAACCCGTGCACCAGCACCAGCGGTGTACCGCCTTCACCCAGCTCGAACCAGCGCAGCAGGCGCCCGCCTACTTCCGCCTTCTGAg               >  2:256271/1‑149 (MQ=255)                                                                                                                   aTGACCCGCCGCTCGGCAGCC‑AGCGCCGGGTGGTTGAACAGCCAGTTGTTGAGGTCACCACCAAACCCGTGCACCAGCACCAGCGGTGTACCGCCTTCACCCAGCTCGAACCAGCGCAGCAGGCGCCCGCCTACTTCCGCCTTCTGAgg              <  5:114996/149‑1 (MQ=255)                                                                                                                    tGACCCGCCGCTCGGCAGCC‑AGCGCCGGGTGGTTGAACAGCCAGTTGTTGAGGTCACCACCAAACCCGTGCACCAGCACCAGCGGTGTACCGCCTTCACCCATCTCGAACCAGCGCAGCAGGCGCCCGCCTACTTCCTCCTTCTGAgg              <  2:141464/148‑1 (MQ=255)                                                                                                                     gACCCGCCGCTCGGCAGCC‑AGCGCCGGGTGGTTGAACAGCCAGTTGTTGAGGTCACCACCAAACCCGTGCACCAGCACCAGCGGTGTACCGCCTTCACCCAGCTCGAACCAGCGCAGCAGGcgcc                                    >  6:144342/1‑125 (MQ=255)                                                                                                                     gACCCGCCGCTCGGCAGCC‑AGCGCCGGGTGGTTGAACAGCCAGTTGTTGAGGTCACCACCAAACCCGTGCACCAGCACCAGCGGTGTACCGCCTTCACCCAGCTCGAACCAGCGCAGCAGGcgcc                                    <  5:144342/125‑1 (MQ=255)                                                                                                                          gccgcTCGGCAGCC‑AGCGCCGGGTGGTTGAACAGCCAGTTGTTGAGGTCACCACCAAACCCGTGCACCAGCACCAGCGGTGTACCGCCTTCACCCAGCTCGAACCAGCGCAGCAGGCGCCCGCCTACTTCCGCCTTCTGAGGggccggc       <  4:288635/149‑1 (MQ=255)                                                                                                                          gccgcTCGGCAGCC‑AGCGCCGGGTGGTTGAACAGCCAGTTGTTGAGGTCACCACCAAACCCGTGCACCAGCACCAGCGGTGTACCGCCTTCACCCAGCTCGAACCAGCGCAGCAGGCGCCCGCCTACTTCCGCCTTCTGAGGggccggc       >  1:194506/1‑149 (MQ=255)                                                                                                                            cgcTCGGCAGCC‑AGCGCCGGGTGGTTGAACAGCCAGTTGTTGAGGTCACCACCAAACCCGTGCACCAGCACCAGCGGTGTACCGCCTTCACCCAGCTCGAACCAGCGCAGCAGGCGCCCGCCTACTTCCGCCTTCTGAGGGGCCGGccc     >  6:129143/1‑149 (MQ=255)                                                                                                                             gcTCGGCAGCC‑AGCGCCGGGTGGTTGAACAGCCAGTTGTTGAGGTCACCACCAAACCCGTGCACCAGCACCAGCGGTGTACCGCCTTCACCCAGCTCGAACCAGCGCAGCAGGCGCCCGCCTACTTCCGCCTTCTGAGGGGCCGGcccc    >  7:151245/1‑149 (MQ=255)                                                                                                                              cTCGGCAGCC‑AGCGCCGGGTGGTTGAACAGCCAGTTGTTGAGGTCACCACCAAACCCGTGCACCAGCACCAGCGGTGTACCGCCTTCACCCAGCTCGAACCAGCGCAGCAGGCGCCCGCCTACTTCCGCCTTCTGAGGGGCCGGCCCCt   <  5:129143/149‑1 (MQ=255)                                                                                                                              cTCGGCAGCC‑AGCGCCGGGTGGTTGAACAGCCAGTTGTTGAGGTCACCACCAAACCCGTGCACCAGCACCAGCGGTGTACCGCCTTCACCCAGCTCGAACCAGCGCAGCAGGCGCCCGCCTACTTCCGCCTTCTGAGGGGCCGGCCCCt   <  8:151245/149‑1 (MQ=255)                                                                                                                                 ggCAGCC‑AGCGCCGGGTGGTTGAACAGCCAGTTGTTGAGGTCACCACCAAACCCGTGCACCAGCACCAGCGGTGTACCGCCTTCACCCAGCTCGAACCAGCGCAGCAGGCGCCCGCCTACTTCCGCCTTCTGAGGGGCCGGCCCCTg  <  7:44058/147‑1 (MQ=255)                                                                                                                                             |                                                                                                                                        GCCTTGGCGATGTCCAGGTGGTCGAGCAGGGCGAGCACGGTTTCGCTCAACTCATCCAGGTCGCCCCGCTGCAGGGCCTTGGCCGACTCGCCGTGGCCCGGCAGGTCGAGGGCGATGACCCGCCGCTCGGCAGCC‑AGCGCCGGGTGGTTGAACAGCCAGTTGTTGAGGTCACCACCAAACCCGTGCACCAGCACCAGCGGTGTACCGCCTTCACCCAGCTCGAACCAGCGCAGCAGGCGCCCGCCTACTTCCGCCTTCTGAGGGGCCGGCCCCTG  >  NC_002947/642388‑642662

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑