| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 58,435 | G→C | 8.6% | intergenic (‑346/+342) | PP_5424 ← / ← PP_0050 | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 58,435 | 0 | G | C | 8.6% | 178.3 / 7.3 | 58 | intergenic (‑346/+342) | PP_5424/PP_0050 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (28/25); new base C (3/2); total (31/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.22e-01 | |||||||||||
CCTCCGTGAGGCCGGCCATCGTCATGCATCAGATCTTGATTGACACGCGTGCCGCTTTGGATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGC‑GGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTACTCCTCATCCCTTACCGAAAAGTCACGCGTCGGTCGTAAACGGCCCTTTC > NC_002947/58290‑58576 | ccTCCGTGAGGCCGGCCATCGTCATGCATCAGATCTTGATTGACACGCGTGCCGCTTTGGATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTg > 5:52015/1‑149 (MQ=255) gTGAGGCCGGCCATCGTCATGCATCAGATCTTGATTGACACGCGTGCCGCTTTGGATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTcc > 6:148740/1‑149 (MQ=255) tGAGGCCGGCCATCGTCATGCATCAGATCTTGATTGACACGCGTGCCGCTTTGGATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCct < 5:148740/149‑1 (MQ=255) aGGCCGGCCATCGTCATGCATCAGATCTTGATTGACACGCGTGCCGCTTTGGATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCctct < 3:122299/149‑1 (MQ=255) gccggccATCGTCATGCATCAGATCTTGATTGACACGCGTGCCGCTTTGGATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGa < 8:28624/149‑1 (MQ=255) ccggccATCGTCATGCATCAGATCTTGATTGACACGCGTGCCGCTTTGGATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGaa < 3:102743/149‑1 (MQ=255) cggccATCGTCATGCATCAGATCTTGATTGACACGCGTGCCGCTTTGGATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAag > 1:96501/1‑149 (MQ=255) gccATCGTCATGCATCAGATCTTGATTGACACGCGTGCCGCTTTGGATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAagta < 6:324679/149‑1 (MQ=255) cATCGTCATGCATCAGATCTTGATTGACACGCGTGCCGCTTTGGATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAagtagt < 1:335034/149‑1 (MQ=255) aTCGTCATGCATCAGATCTTGATTGACACGCGTGCCGCTTTGGATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTg < 2:96501/149‑1 (MQ=255) tGCATCAGATCTTGATTGACACGCGTGCCGCTTTGGATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGAcggc‑gg > 1:19418/1‑149 (MQ=255) gCATCAGATCTTGATTGACACGCGTGCCGCTTTGGATAGCCTTGAAATTGAGGGCTGAAAATGCGCATTTGTCGGTCCTGGGTACAAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGC‑ggg > 1:60965/1‑149 (MQ=255) cATCAGATCTTGATTGACACGCGTGCCGCTTTGGATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGC‑ggg < 2:19418/148‑1 (MQ=255) tCAGATCTTGATTGACACGCGTGCCGCTTTGGATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTc > 7:295011/1‑125 (MQ=255) tCAGATCTTGATTGACACGCGTGCCGCTTTGGATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTc < 8:295011/125‑1 (MQ=255) cAGATCTTGATTGACACGCGTGCCGCTTTGGATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGC‑GGGCAtt > 7:15189/1‑149 (MQ=255) cAGATCTTGATTGACACGCGTGCCGCTTTGGATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAA‑TTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGC‑GGGCATTg > 3:361992/1‑149 (MQ=255) gattgaCACGCGTGCCGCTTTGGATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAACCAGCTTCCTCTgct > 6:254700/1‑121 (MQ=255) gattgaCACGCGTGCCGCTTTGGATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAACCAGCTTCCTCTgct < 5:254700/123‑3 (MQ=255) gattgaCACGCGTGCCGCTTGGGATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGC‑GGGCATTGCCAGTc < 4:26234/148‑1 (MQ=255) acacGCGTGCCGCTTTGGATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGC‑GGGCATTGCCAGTCGGATTc < 2:125320/149‑1 (MQ=255) cacGCGTGCCGCTTTGGATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGC‑GGGCATTGCCAGTCGGATTCt < 5:7474/149‑1 (MQ=255) gcgTGCCGCTTTGGATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGC‑GGGCATTGCCAGt < 7:10235/138‑1 (MQ=255) gcgTGCCGCTTTGGATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGC‑GGGCATTGCCAGt > 8:10235/1‑138 (MQ=255) tGCCGCTTTGGATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGC‑GGGCATTGCCAGTCGGATTCTGAAcc > 6:100120/1‑148 (MQ=255) cTTTGGATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGC‑GGGCATTGCCAGTCGGATTCTGAACCCAAGGt > 5:95557/1‑149 (MQ=255) ccTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGC‑GGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGAc > 8:62940/1‑147 (MQ=255) cTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGC‑GGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAg < 8:15189/149‑1 (MQ=255) ctaTTGAGGGCTGCAACGGCTCTTGTGTCGGGCATGGGTAGGAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGC‑GGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAg < 6:74863/146‑1 (MQ=255) aGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGC‑GGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCTAAAg < 6:52015/148‑1 (MQ=255) aTTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGC‑GGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTActc > 3:74611/1‑149 (MQ=255) tCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGC‑GGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTACTCCTCAt > 5:65255/1‑149 (MQ=255) gggCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGC‑GGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCTAAAGGATc < 4:275480/129‑1 (MQ=255) gggCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGC‑GGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCTAAAGGATc > 3:275480/1‑129 (MQ=255) gggTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGC‑GGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTActcc > 5:323/1‑137 (MQ=255) gggTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGC‑GGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTActcc < 6:323/137‑1 (MQ=255) aTGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGC‑GGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTACTCCTCATCCCTTACCGAAAAGTCAc < 2:60965/149‑1 (MQ=255) tGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGC‑GGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTACTCCTCATCCCTTACCGAAAAGTCAc < 8:233174/148‑1 (MQ=255) tGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGC‑GGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTACTCCTCATCCCTTACCGAAAAGTCAc > 7:233174/1‑148 (MQ=255) aGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGC‑GGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTACTCCTCATCCCTTACCGAAAAGTCAcgc < 7:62940/148‑1 (MQ=255) cccGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTACTCCTCATCCCTTACCGAAAAGTCACGCgtcg > 1:91907/1‑149 (MQ=255) ccGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGC‑GGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTACTCCTCATCCCTTACCGAAAAGTCACGCgtcggt > 1:166125/1‑149 (MQ=255) ccGCAA‑TTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGC‑GGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTACTCCTCATCCCTTACCGAAAAGTCACGCgtcggtc < 4:361992/149‑1 (MQ=255) cGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGC‑GGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTACTCCTCATCCCTTACCGAAAAGTCACGCgtcggtc > 2:331792/1‑149 (MQ=255) cagcagAGGAAGCTGGTTCCTCTGAAGTAGTGACGGC‑GGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTACTCTTCATCCCTTAc < 2:368954/118‑1 (MQ=255) cagcagAGGAAGCTGGTTCCTCTGAAGTAGTGACGGC‑GGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTACTCTTCATCCCTTAc > 1:368954/1‑118 (MQ=255) ttcagAGGAACCAGCTTCCTCTGAAGTAGTGACGGC‑GGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTACTCCTCATCCCTTACCGAAAAGTCACGCGTCGGTCGTAAACGGCCCt > 3:229172/3‑149 (MQ=255) agcagAGGAAGCTGGTTCCTCTGAAGTAGTGACGGC‑GGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTACTCCTCATCCCTTACCGAAAAGTCACGCGTCGGTCGTAAACGGCCCt > 8:298430/1‑149 (MQ=255) agcagAGGAAGCTGGTTCCTCTGAAGTAGTGACGGC‑GGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTACTCCTCATCCCTTACCGAAAAGTCACGCGTCGGTCGTAAACGGCCCt > 4:371102/1‑149 (MQ=255) gcagAGGAAGCTGGTTCCTCTGAAGTAGTGACGGC‑GGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTACTCCTCATCCCTTACCGaaa < 3:172193/121‑1 (MQ=255) gcagAGGAAGCTGGTTCCTCTGAAGTAGTGACGGC‑GGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTACTCCTCATCCCTTACCGaaa > 4:172193/1‑121 (MQ=255) cagAGGAACCAGCTTCCTCTGAAGTAGTGACGGC‑GGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGa > 6:200680/1‑97 (MQ=255) cagAGGAACCAGCTTCCTCTGAAGTAGTGACGGC‑GGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGa < 5:200680/97‑1 (MQ=255) gagGAAGCTGGTTCCTCTGAAGTAGTGACGGC‑GGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTACTCCTCATCCCTTACCGAAAAGTCACGCGTCGGTCGTAAACGGCCCTTTc > 8:338679/1‑148 (MQ=255) agGAAGCTGGTTCCTCTGAAGTAGTGACGGC‑GGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTACTCCTCATCCCTTACCGAAAAGTCACGCGTCGGTCGTAAACGGCCCTTTc > 6:154297/1‑147 (MQ=255) agGAAGCTGGTTCCTCTGAAGTAGTGACGGC‑GGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTACTCCTCATCCCTTACCGAAAAGTCACGCGTCGGTCGTAAACGGCCCTTTc > 1:335969/1‑147 (MQ=255) gAAGCTGGTTCCTCTGAAGTAGTGACGGC‑GGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGcc < 3:303573/65‑1 (MQ=255) gAAGCTGGTTCCTCTGAAGTAGTGACGGC‑GGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGcc > 4:303573/1‑65 (MQ=255) | CCTCCGTGAGGCCGGCCATCGTCATGCATCAGATCTTGATTGACACGCGTGCCGCTTTGGATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGC‑GGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTACTCCTCATCCCTTACCGAAAAGTCACGCGTCGGTCGTAAACGGCCCTTTC > NC_002947/58290‑58576 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |