Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	714,958	G→A	9.4%	intergenic (+24/+53)	ispH → / ← PP_0607	4‑hydroxy‑3‑methylbut‑2‑enyl diphosphate reductase/type IV pili biogenesis protein FimT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	714,958	0	G	A	9.4%	146.8 / 5.0	53	intergenic (+24/+53)	ispH/PP_0607	4‑hydroxy‑3‑methylbut‑2‑enyl diphosphate reductase/type IV pili biogenesis protein FimT
Reads supporting (aligned to +/- strand):  ref base G (23/25);  new base A (3/2);  total (26/27)
Fisher's exact test for biased strand distribution p-value = 6.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.68e-01

GTGCGTGGCGTGATCGAACAGCTCAAGGCCTGGGGCGCCACTGGCGCTGAAGAGCTGGACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGATATCGGCTGCGAGCAGCGGCGCG  >  NC_002947/714812‑715103                                                                                                                                                   |                                                                                                                                                   gTGCGTGGCGTGATCGAACAGCTCAAGGCCTGGGGCGCCACTGGCGCTGAAGAGCTGGACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAggg                                                                                                                                                  >  4:279052/1‑149 (MQ=255) gTGCGTGGCGTGATCGAACAGCTCAAGGCCTGGGGCGCCACTGGCGCTGAAGAGCTGGACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAg                                                                                                                                                    <  5:324601/147‑1 (MQ=255)     gtggcgtgATCGAACAGCTCAAGGCCTGGGGCGCCACTGGCGCTGAAGAGCTGGACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGgctg                                                                                                                                              <  7:230579/149‑1 (MQ=255)        gggtgATCGAACAGCTCAAGGGCTGGTGCTCCACTGGGGCTGAAGAGCTGGACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCtt                                                                                                                                           <  3:20763/147‑1 (MQ=255)             aTCGAACAGCTCAAGGCCTGGGGCGCCACTGGCGCTGAAGAGCTGGACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCa                                                                                                                                       <  4:103428/148‑1 (MQ=255)             aTCGAACAGCTCAAGGCCTGGGGCGCCACTGGCGCTGAAGAGCTGGACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCa                                                                                                                                       <  6:243200/148‑1 (MQ=255)                gAACAGCTCAAGGCCTGGGGCGCCACTGGCGCTGAAGAGCTGGACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAAGGGCTGCAAAGCAGccc                                                                                                                                   <  8:20716/149‑1 (MQ=255)                  aCAGCTCAAGGCCTGGGGCGCCACTGGCGCTGAAGAGCTGGACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCtt                                                                                                                                 >  6:213234/1‑149 (MQ=255)                     gCTCAAGGCCTGGGGCGCCACTGGCGCTGAAGAGCTGGACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAAGGGCTGCAAAGCAGCCCCTgca                                                                                                                              <  7:295463/149‑2 (MQ=255)                      cTCAAGGCCTGGGGCGTCACTGGCGCTGAAGAGCTGGACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGACAGTACCGCCGCCAATGCAg                                                                                                                                                    <  5:327158/126‑1 (MQ=255)                      cTCAAGGCCTGGGGCGTCACTGGCGCTGAAGAGCTGGACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGACAGTACCGCCGCCAATGCAg                                                                                                                                                    >  6:327158/1‑126 (MQ=255)                       tCAAGGCCTGGGGCGCCACTGGCGCTGAAGAGCTGGACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTgcgc                                                                                                                             >  5:96819/1‑148 (MQ=255)                        cAAGGCCTGGGGCGCCACTGGCGCTGAAGAGCTGGACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGa                                                                                                                           >  4:77177/1‑149 (MQ=255)                         aaGGCCTGGGGCGCCACTGGCGCTGAAGAGCTGGACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCttt                                                                                                                                          <  7:302862/133‑1 (MQ=255)                         aaGGCCTGGGGCGCCACTGGCGCTGAAGAGCTGGACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCttt                                                                                                                                          >  8:302862/1‑133 (MQ=255)                         aaGGCCTGGGGCGCCACTGGCGCTGAAGAGCTGGACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGa                                                                                                                           <  3:279052/148‑1 (MQ=255)                             ccTGGGGCGCCACTGGCGCTGAAGAGCTGGACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACaa                                                                                                                      <  6:96819/149‑1 (MQ=255)                                   gcgcCACTGGCGCTGAAGAGCTGGACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTgcgc                                                                                                                             >  2:165362/1‑136 (MQ=255)                                   gcgcCACTGGCGCTGAAGAGCTGGACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTgcgc                                                                                                                             <  1:165362/136‑1 (MQ=255)                                      ccACTGGCGCTGAAGAGCTGGACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTcc                                                                                                             >  3:369310/1‑149 (MQ=255)                                        aCTGGCGCTGAAGAGCTGGACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTg                                                                                                           >  2:350248/1‑149 (MQ=255)                                         cTGGCGCTGAAGAGCTGGACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGTGACACAAGACCGCTCCTGc                                                                                                          <  8:254235/149‑1 (MQ=255)                                            gcgcTGAAGAGCTGGACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCaa                                                                                                        <  5:213234/148‑1 (MQ=255)                                              gcTGAAGAGCTGGACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGa                                                                                                     <  3:77177/149‑1 (MQ=255)                                                  aaGAGCTGGACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACcg                                                                                                  <  7:119224/148‑1 (MQ=255)                                                          gACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTagcag                                                                                        >  3:284526/1‑149 (MQ=255)                                                             gggCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTa                                                                                            >  6:76203/1‑142 (MQ=255)                                                             gggCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTa                                                                                            <  5:76203/142‑1 (MQ=255)                                                             gggCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCgg                                                                                     <  1:350248/149‑1 (MQ=255)                                                                    aaGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGCTGCTAGC‑GCGGTCATTGc                                                                              <  5:124303/149‑1 (MQ=255)                                                                     agagAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGc                                                                              >  1:327583/1‑148 (MQ=255)                                                                      gagaACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTc                                                                            <  2:327583/149‑1 (MQ=255)                                                                            aTCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGtcc                                                                      >  1:345830/1‑149 (MQ=255)                                                                              cACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAg                                                                          >  7:77051/1‑143 (MQ=255)                                                                              cACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAg                                                                          <  8:77051/143‑1 (MQ=255)                                                                               aCCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGtcctcc                                                                   >  5:224093/1‑149 (MQ=255)                                                                                            ccAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCgc‑gc                                                                                              <  2:138643/109‑1 (MQ=255)                                                                                            ccAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCgc‑gc                                                                                              >  1:138643/1‑109 (MQ=255)                                                                                             cAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCg                                                                                      <  6:158712/116‑1 (MQ=255)                                                                                             cAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCg                                                                                      >  5:158712/1‑116 (MQ=255)                                                                                             cAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGAc                                                     >  8:133681/1‑149 (MQ=255)                                                                                              aaaGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGAc                                                     >  8:32232/1‑148 (MQ=255)                                                                                                        gggTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACCCTGCCGCTTg                                          <  7:133681/149‑1 (MQ=255)                                                                                                          gTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGAt                                        <  4:284526/149‑1 (MQ=255)                                                                                                              gctcgctGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGc                                      >  3:261174/1‑147 (MQ=255)                                                                                                                  gctGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCACTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGcac                                >  3:237288/1‑149 (MQ=255)                                                                                                                            cGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGatat                      <  6:224093/149‑1 (MQ=255)                                                                                                                             gggCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGATATc                     >  1:284784/1‑149 (MQ=255)                                                                                                                                cAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGATATCGGc                  <  7:32232/149‑1 (MQ=255)                                                                                                                                  gTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGATATCGGCTg                <  4:261174/149‑1 (MQ=255)                                                                                                                                  gTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTACTGAAATGACCGA‑GCTAGCAGCGGTCAATGCTCATGTCATCCGTAAGCAACCTTACTCTGCCGCTTTATGCCCGCACAACCTGATATCGGCTg                >  6:366801/1‑149 (MQ=255)                                                                                                                                         cgccAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGATATCGGCTGCGagcag         >  2:21340/1‑149 (MQ=255)                                                                                                                                          gccAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGActc                                                   <  3:82348/106‑1 (MQ=255)                                                                                                                                          gccAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGActc                                                   >  4:82348/1‑106 (MQ=255)                                                                                                                                            tcgcgCAAGGGCTGCAAAGCAGCCCCTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGATATCGGCTGCGAGCAgcgg      >  8:349180/5‑149 (MQ=255)                                                                                                                                                gCAAGGGCTGCAAAGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGATATCGGCTGCGAGCAGCGGcgcg  >  6:327969/1‑149 (MQ=255)                                                                                                                                                gCAAGGGCTGCAAAGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGATATCGGCTGCGAGCAGCGGcgcg  >  3:209281/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                   GTGCGTGGCGTGATCGAACAGCTCAAGGCCTGGGGCGCCACTGGCGCTGAAGAGCTGGACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGATATCGGCTGCGAGCAGCGGCGCG  >  NC_002947/714812‑715103

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑