Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	714,968	T→A	9.3%	intergenic (+34/+43)	ispH → / ← PP_0607	4‑hydroxy‑3‑methylbut‑2‑enyl diphosphate reductase/type IV pili biogenesis protein FimT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	714,968	0	T	A	9.3%	153.3 / 6.1	54	intergenic (+34/+43)	ispH/PP_0607	4‑hydroxy‑3‑methylbut‑2‑enyl diphosphate reductase/type IV pili biogenesis protein FimT
Reads supporting (aligned to +/- strand):  ref base T (26/23);  new base A (3/2);  total (29/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.85e-01

ATCGAACAGCTCAAGGCCTGGGGCGCCACTGGCGCTGAAGAGCTGGACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGATATCGGCTGCGAGCAGCGGCGCGTTCGCACATTT  >  NC_002947/714824‑715114                                                                                                                                                 |                                                                                                                                                    aTCGAACAGCTCAAGGCCTGGGGCGCCACTGGCGCTGAAGAGCTGGACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCa                                                                                                                                                  <  6:243200/148‑1 (MQ=255) aTCGAACAGCTCAAGGCCTGGGGCGCCACTGGCGCTGAAGAGCTGGACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCa                                                                                                                                                  <  4:103428/148‑1 (MQ=255)    gAACAGCTCAAGGCCTGGGGCGCCACTGGCGCTGAAGAGCTGGACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAAGGGCTGCAAAGCAGccc                                                                                                                                              <  8:20716/149‑1 (MQ=255)      aCAGCTCAAGGCCTGGGGCGCCACTGGCGCTGAAGAGCTGGACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCtt                                                                                                                                            >  6:213234/1‑149 (MQ=255)         gCTCAAGGCCTGGGGCGCCACTGGCGCTGAAGAGCTGGACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAAGGGCTGCAAAGCAGCCCCTgca                                                                                                                                         <  7:295463/149‑2 (MQ=255)           tCAAGGCCTGGGGCGCCACTGGCGCTGAAGAGCTGGACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTgcgc                                                                                                                                        >  5:96819/1‑148 (MQ=255)            cAAGGCCTGGGGCGCCACTGGCGCTGAAGAGCTGGACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGa                                                                                                                                      >  4:77177/1‑149 (MQ=255)             aaGGCCTGGGGCGCCACTGGCGCTGAAGAGCTGGACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCttt                                                                                                                                                     >  8:302862/1‑133 (MQ=255)             aaGGCCTGGGGCGCCACTGGCGCTGAAGAGCTGGACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCttt                                                                                                                                                     <  7:302862/133‑1 (MQ=255)             aaGGCCTGGGGCGCCACTGGCGCTGAAGAGCTGGACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGa                                                                                                                                      <  3:279052/148‑1 (MQ=255)                 ccTGGGGCGCCACTGGCGCTGAAGAGCTGGACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACaa                                                                                                                                 <  6:96819/149‑1 (MQ=255)                       gcgcCACTGGCGCTGAAGAGCTGGACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTgcgc                                                                                                                                        <  1:165362/136‑1 (MQ=255)                       gcgcCACTGGCGCTGAAGAGCTGGACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTgcgc                                                                                                                                        >  2:165362/1‑136 (MQ=255)                          ccACTGGCGCTGAAGAGCTGGACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTcc                                                                                                                        >  3:369310/1‑149 (MQ=255)                            aCTGGCGCTGAAGAGCTGGACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTg                                                                                                                      >  2:350248/1‑149 (MQ=255)                             cTGGCGCTGAAGAGCTGGACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGTGACACAAGACCGCTCCTGc                                                                                                                     <  8:254235/149‑1 (MQ=255)                                gcgcTGAAGAGCTGGACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCaa                                                                                                                   <  5:213234/148‑1 (MQ=255)                                  gcTGAAGAGCTGGACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGa                                                                                                                <  3:77177/149‑1 (MQ=255)                                      aaGAGCTGGACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACcg                                                                                                             <  7:119224/148‑1 (MQ=255)                                              gACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTagcag                                                                                                   >  3:284526/1‑149 (MQ=255)                                                 gggCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTa                                                                                                       >  6:76203/1‑142 (MQ=255)                                                 gggCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTa                                                                                                       <  5:76203/142‑1 (MQ=255)                                                 gggCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCgg                                                                                                <  1:350248/149‑1 (MQ=255)                                                        aaGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGCTGCTAGC‑GCGGTCATTGc                                                                                         <  5:124303/149‑1 (MQ=255)                                                         agagAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGc                                                                                         >  1:327583/1‑148 (MQ=255)                                                          gagaACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTc                                                                                       <  2:327583/149‑1 (MQ=255)                                                                aTCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGtcc                                                                                 >  1:345830/1‑149 (MQ=255)                                                                  cACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAg                                                                                     >  7:77051/1‑143 (MQ=255)                                                                  cACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAg                                                                                     <  8:77051/143‑1 (MQ=255)                                                                   aCCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGtcctcc                                                                              >  5:224093/1‑149 (MQ=255)                                                                                ccAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCgc‑gc                                                                                                         >  1:138643/1‑109 (MQ=255)                                                                                ccAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCgc‑gc                                                                                                         <  2:138643/109‑1 (MQ=255)                                                                                 cAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCg                                                                                                 <  6:158712/116‑1 (MQ=255)                                                                                 cAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCg                                                                                                 >  5:158712/1‑116 (MQ=255)                                                                                 cAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGAc                                                                >  8:133681/1‑149 (MQ=255)                                                                                  aaaGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGAc                                                                >  8:32232/1‑148 (MQ=255)                                                                                            gggTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACCCTGCCGCTTg                                                     <  7:133681/149‑1 (MQ=255)                                                                                              gTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGAt                                                   <  4:284526/149‑1 (MQ=255)                                                                                                  gctcgctGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGc                                                 >  3:261174/1‑147 (MQ=255)                                                                                                      gctGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCACTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGcac                                           >  3:237288/1‑149 (MQ=255)                                                                                                                cGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGatat                                 <  6:224093/149‑1 (MQ=255)                                                                                                                 gggCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGATATc                                >  1:284784/1‑149 (MQ=255)                                                                                                                    cAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGATATCGGc                             <  7:32232/149‑1 (MQ=255)                                                                                                                      gTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGATATCGGCTg                           <  4:261174/149‑1 (MQ=255)                                                                                                                      gTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTACTGAAATGACCGA‑GCTAGCAGCGGTCAATGCTCATGTCATCCGTAAGCAACCTTACTCTGCCGCTTTATGCCCGCACAACCTGATATCGGCTg                           >  6:366801/1‑149 (MQ=255)                                                                                                                             cgccAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGATATCGGCTGCGagcag                    >  2:21340/1‑149 (MQ=255)                                                                                                                              gccAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGActc                                                              <  3:82348/106‑1 (MQ=255)                                                                                                                              gccAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGActc                                                              >  4:82348/1‑106 (MQ=255)                                                                                                                                tcgcgCAAGGGCTGCAAAGCAGCCCCTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGATATCGGCTGCGAGCAgcgg                 >  8:349180/5‑149 (MQ=255)                                                                                                                                    gCAAGGGCTGCAAAGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGATATCGGCTGCGAGCAGCGGcgcg             >  3:209281/1‑149 (MQ=255)                                                                                                                                    gCAAGGGCTGCAAAGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGATATCGGCTGCGAGCAGCGGcgcg             >  6:327969/1‑149 (MQ=255)                                                                                                                                          gctgctTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGATATCGGCTGCGAGCAGCGGCGCGTTCGca       >  8:28194/1‑149 (MQ=255)                                                                                                                                            tgctTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGc                                                        <  3:344391/98‑1 (MQ=255)                                                                                                                                            tgctTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGc                                                        >  4:344391/1‑98 (MQ=255)                                                                                                                                            tgctTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGATATCGGCTGCGAGCAGCGGCGCGTTCGcaca     >  6:201796/1‑149 (MQ=255)                                                                                                                                              ctTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGATATCGGCTGCGAGCAGCGGCGCGTTCGCACAtt   >  6:332688/1‑149 (MQ=255)                                                                                                                                               tttGCAGCCCTTGCGCGACACAAGACCACTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTTCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGATATCGGCTGCGAGCAGCGGCGCGTTCGCACAttt  <  4:237288/149‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                    ATCGAACAGCTCAAGGCCTGGGGCGCCACTGGCGCTGAAGAGCTGGACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGATATCGGCTGCGAGCAGCGGCGCGTTCGCACATTT  >  NC_002947/714824‑715114

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑