Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 772,178 T→C 8.0% intergenic (+39/‑160) PP_0663 → / → PP_0664 AsnC family transcriptional regulator/homoserine dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947772,1780TC 8.0% 172.3 / 3.3 62intergenic (+39/‑160)PP_0663/PP_0664AsnC family transcriptional regulator/homoserine dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (29/28);  new base C (3/2);  total (32/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGTCGAGGCCTCCAGCCCGGAGCGGGTGCACCATGTGTGGAAGGAAATCGCTGCCATGCCCGGGGTGGAGAACACGGTGACTTCGTTCGTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGCCATATTCAACTGGCAGTGCACGGCTCTTACGCTAGTCCTCATCGACCCACTGCCTGGACAG  >  NC_002947/772030‑772326
                                                                                                                                                    |                                                                                                                                                    
gcgTCGAGGCCTCCAGCCCGGAGCGGGTGCACCATGTGTGGAAGGAAATCGCTGCCATGCCCGGGGTGGAGAACACGGTGACTTCGTTCGTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGt                                                                                                                                                      >  5:98996/1‑149 (MQ=255)
         ccTCCAGCCCGGAGCGGGTGCAGCATGTGTGGAAGGAAATCGCTGCCATGCCCGGGGGGGAGAACACGGTGACTTCGTTCGTCCTGGCCGCGGTGGGCGGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCa                                                                                                                                             <  7:266508/149‑1 (MQ=255)
          cTCCAGCCCGGAGCGGGTGCACCATGTGTGGAAGGAAATCGCTGCCATGCCCGGGGTGGAGAACACGGTGACTTCGTTCGTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAg                                                                                                                                            >  1:125664/1‑149 (MQ=255)
          cTCCAGCCCGGAGCGGGTGCACCATGTGTGGAAGGAAATCGCTGCCATGCCCGGGGTGGAGAACACGGTGACTTCGTTCGTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAg                                                                                                                                            >  7:142133/1‑149 (MQ=255)
          cTCCAGCCCGGAGCGGGTGCACCATGTGTGGAAGGAAATCGCTGCCATGCCCGGGGTGGAGAACACGGTGACTTCGTTCGTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAg                                                                                                                                            >  3:89092/1‑149 (MQ=255)
           tCCAGCCCGGAGCGGGTGCACCATGTGTGGAAGGAAATCGCTGCCATGCCCGGGGTGGAGAACACGGTGACTTCGTTCGTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAgg                                                                                                                                           >  3:121209/1‑149 (MQ=255)
           tCCAGCCCGGAGCGGGTGCACCATGTGTGGAAGGAAATCGCTGCCATGCCCGGGGTGGAGAACACGGTGACTTCGTTCGTCCTCGCCGCGGTGAACTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAgg                                                                                                                                           >  1:119064/1‑149 (MQ=255)
             cAGCCCGGAGCGGGTGCACCATGTGTGGAAGGAAATCGCTGCCATGCCCGGGGTGGAGAACACGGTGACTTCGTTCGTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGCGCGAACCCAggcg                                                                                                                                         >  2:90611/1‑149 (MQ=255)
               gCCCGGAGCGGGTGCACCATGTGTGGAAGGAAATCGCTGCCATGCCCGGGGTGGAGAACACGGTGACTTCGTTCGTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGCGCGAACCCAggcggc                                                                                                                                       <  1:90611/149‑1 (MQ=255)
               gCCCGGAGCGGGTGCACCATGTGTGGAAGGAAATCGCTGCCATGCCCGGGGTGGAGAACACGGTGACTTCGTTCGTCCTGGCCGCGGTGGGCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAggcggc                                                                                                                                       <  5:222701/149‑1 (MQ=255)
                  cGGAGCGGGTGCACCATGTGTGGAAGGAAATCGCTGCCATGCCCGGGGTGGAGAACACGGTGACTTCGTTCGTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGtt                                                                                                                                    <  4:89092/149‑1 (MQ=255)
                   ggAGCGGGTGCACCATGTGTGGAAGGAAATCGCTGCCATGCCCGGGGTGGAGAACACGGTGACTTCGTTCGTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTtg                                                                                                                                   >  1:136487/1‑149 (MQ=255)
                           tGCACCATGTGTGGAAGGAAATCGCTGCCATGCCCGGGGTGGAGAACACGGTGACTTCGTTCGTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGtttt                                                                                                                           <  6:330405/149‑1 (MQ=255)
                                 aTGCGTCGAAGGAACTCGCGGCCCTGCCCGGGGTGGAGAACACGGTGACTTCGTTCGTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTg                                                                                                                     <  8:274774/149‑1 (MQ=255)
                                  tgtgtgGAAGGAAATCGCTGCCATGCCCGGGGTGGAGAACACGGTGACTTCGTTCGTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTgg                                                                                                                    <  8:304018/149‑1 (MQ=255)
                                          aggaaATCGCTGCCATGCCCGGGGTGGAGAACACGGTGACTTCGTTCGTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTg                                                                                                            >  3:295357/1‑149 (MQ=255)
                                            gaaATCGCTGCCATGCCCGGGGTGGAGAACACGGTGACTTCGTTCGTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTgcg                                                                                                          >  5:329823/1‑149 (MQ=255)
                                             aaaTCGCTGCCATGCCCGGGGTGGAGAACACGGTGACTTCGTTCGTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTgcgc                                                                                                         <  6:98996/149‑1 (MQ=255)
                                                  gCTGCCATGCCCGGGGTGGAGAACACGGTGACTTCGTTCGTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCttt                                                                                                    <  5:212976/149‑1 (MQ=255)
                                                      ccATGCCAGGGGTGGAGAACACGGTGACTTCGTTCGTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGCGCGAACCCAGgcggcg                                                                                                                                      <  6:265747/111‑1 (MQ=38)
                                                      ccATGCCAGGGGTGGAGAACACGGTGACTTCGTTCGTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGCGCGAACCCAGgcggcg                                                                                                                                      >  5:265747/1‑111 (MQ=38)
                                                             cGGGGTGGAGAACACGGTGACTTCGTTCGTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTCAATTGGATAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTGGGGGGTTTTGCCATGGCGCAGGTGCGCGCTTTGCAATGGCCa                                                                                          >  8:173550/1‑148 (MQ=255)
                                                                    gagaACACGGTGACTTCGTTCGTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGc                                                                                   >  1:332796/1‑148 (MQ=255)
                                                                      gaACACGGTGACTTCGTTCGTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAATCTGGCCag                                                                                <  4:295357/149‑2 (MQ=255)
                                                                       cacacGGGGACTTCCGTCGGCCTGGCCGCGGGGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAATCTGGCCagt                                                                               <  7:173550/148‑3 (MQ=255)
                                                                       aaCACGGTGACTTCGTTCGTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAATCTGGCCagt                                                                               <  2:125664/149‑3 (MQ=255)
                                                                              tGACTTCGTTCGTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAAAAAATACa                                                                        <  2:298330/149‑1 (MQ=255)
                                                                                  ttcgttcgTCCTGGCCGCGCTGGTCTGAACCGCGTCTTTCCATTGGATATCACAACGCCGCCTGGATTCGCGCCCACGCGGCGTTGTGCGTTTTTCCCTGGCGAAGGTGCGCGCTTTGCACTGGCCACATTGGCCAATAAATACAGCg                                                                     >  3:213809/1‑148 (MQ=255)
                                                                                     gttcgtCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGAtt                                                                 >  3:15805/1‑149 (MQ=255)
                                                                                         gtCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGcc                                                              <  3:15003/148‑1 (MQ=255)
                                                                                          tCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGCCa                                                             >  8:116709/1‑148 (MQ=255)
                                                                                           ccTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGCCata                                                           >  7:27383/1‑149 (MQ=255)
                                                                                             tGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGCCatat                                                          >  6:107615/1‑148 (MQ=255)
                                                                                               gCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGcc                                                                                  <  2:336979/122‑1 (MQ=255)
                                                                                               gCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGcc                                                                                  >  1:336979/1‑122 (MQ=255)
                                                                                               gCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGCCATAtt                                                         <  8:142133/147‑1 (MQ=255)
                                                                                                ccGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGCCATATTCaa                                                      <  6:329823/149‑1 (MQ=255)
                                                                                                 cgcgGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGCCATATTCAAc                                                     <  7:201137/149‑1 (MQ=255)
                                                                                                 cgcgGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGCCATATTCAAc                                                     >  8:201137/1‑149 (MQ=255)
                                                                                                      tggtCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGCCATATTCAACTgg                                                  >  3:239360/1‑147 (MQ=255)
                                                                                                              aCCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGCCATATTCAACTGGCAGTGCACgg                                        >  8:171188/1‑149 (MQ=255)
                                                                                                                cgcgTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGCCATATTCAACt                                                    <  6:244971/135‑1 (MQ=255)
                                                                                                                cgcgTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGCCATATTCAACt                                                    >  5:244971/1‑135 (MQ=255)
                                                                                                                cgcgTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGCATTGCCATATTCAACTGGCAGTGCACGGc                                       >  4:26559/1‑148 (MQ=255)
                                                                                                                   gTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGCCATATTCAACt                                                    <  7:6388/132‑1 (MQ=255)
                                                                                                                   gTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGCCATATTCAACt                                                    >  8:6388/1‑132 (MQ=255)
                                                                                                                        tGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGCCATATTCAACTGGCAGTGCACGGCTCTTAc                                 <  4:15805/146‑1 (MQ=255)
                                                                                                                        tGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGCCATATTCAACTGGCAGTGCACGGCTCTTACGc                               <  4:121209/148‑1 (MQ=255)
                                                                                                                        tGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGCCATATTCAACTGGCAGTGCACGGCTCTTACGc                               <  2:136487/148‑1 (MQ=255)
                                                                                                                           aTTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGCCATATTCAACTGGCAGTGCACGGct                                      >  8:40507/1‑138 (MQ=255)
                                                                                                                           aTTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGCCATATTCAACTGGCAGTGCACGGct                                      <  7:40507/138‑1 (MQ=255)
                                                                                                                           aTTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGCCATATTCAACTGGCAGTGCACGGCTCTTACGCTAGt                           >  1:196910/1‑149 (MQ=255)
                                                                                                                               gCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGCCATATTCAACTGGCAGTGCACGGCTCTTACGCTAGTCCt                        >  2:181707/1‑148 (MQ=255)
                                                                                                                                   gCACTACGCGGAGTGGGTTCGCGGCCAGGCGGCGTTGTGGGTTTTGCCCTGTGGCAGGTGCGCGCTTTGCTCTGGCAAGATTTTCCAATAAATACAGCGGATTGCCATATTCAACTGGCAGTTCACGGCTCTTAGGCTTGTCCTCATc                    <  4:213809/148‑1 (MQ=255)
                                                                                                                                   gCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCTATAAATACAGCGGATTGCCATATTCAACTGGCAGTGCACGGCTCTTACGCTAGTCCTCAt                     <  2:119064/147‑1 (MQ=255)
                                                                                                                                      caACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGCCATATTCAACTGGCAGTGCACGGCTCTTACGCTAGTCCTCATCGAcc                >  7:40223/1‑149 (MQ=255)
                                                                                                                                       aaCGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGCCATATTCAACTGGCAGTGCACGGCTCTTACGCTAGTCCTCATCGAcc                >  5:10975/1‑148 (MQ=255)
                                                                                                                                       aaCGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGCCATATTCAACTGGCAGTGCACGGCTCTTACGCTAGTCCTCATCGAcc                >  8:42208/1‑148 (MQ=255)
                                                                                                                                         cgccgcCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGCCATATTCAACTGGCAGTGCACGGCTCATACGCTAGTCCTCATCgccaca              >  8:346957/1‑143 (MQ=255)
                                                                                                                                           ccgccTGGGCGCGAACCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGCCATATTCAACTGGCAGAGCACGTCTCTTACGCTAGTCCTCATCGACCCAcag           >  8:152246/1‑147 (MQ=255)
                                                                                                                                            cgccTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGCCATATTCAACTGGCAGTGCACGGCTCTTACGCTAGTCCTCATCGACCCACTGc          <  8:27383/149‑1 (MQ=255)
                                                                                                                                               cTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGCCATATTCAACTGGCAGTGCACGGCTCTTACGCTAGTCCTCATCGACCCACTGCCTg       <  1:181707/149‑1 (MQ=255)
                                                                                                                                                  ggTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGCCATATTCAACTGGCAGTGCACGGCTCTTACGCTAGTCCTCATCGACCCACTGCCTgga     <  7:171188/148‑1 (MQ=255)
                                                                                                                                                    ttCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGCCATATTCAACTGGCAGTGCACGGCTCTTACGCTAGTCCTCATCGACCCACTGCCTggacag  <  7:116709/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCGTCGAGGCCTCCAGCCCGGAGCGGGTGCACCATGTGTGGAAGGAAATCGCTGCCATGCCCGGGGTGGAGAACACGGTGACTTCGTTCGTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGCCATATTCAACTGGCAGTGCACGGCTCTTACGCTAGTCCTCATCGACCCACTGCCTGGACAG  >  NC_002947/772030‑772326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑