Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 791,847 T→C 7.6% H401R (CAT→CGT)  PP_0678 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947791,8470TC 7.6% 148.6 / 2.7 54H401R (CAT→CGT) PP_0678hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (26/23);  new base C (2/2);  total (29/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

CAGCTTCAATTGCAGGATTTCGATCTGTGCGCCCAGCGGGAAGCCATAGGCGTTGTCATTCAGGTCGTGGTAGGCCATGCGCAAGCCATATTCGGCGTAGGCGCGGTCTTCACGGGTGCCCACGCCCAGTTGCCAGGTGCGCGAGTCATGGCCATCTTCGGGCAGGCCGGGGCGCTCGATCTGCAGGGGCGGCGGCGGGTTGCTGTTGATCGCCCGCAGCAACTCGAAGCTGCGTTTGGCTTGCCCTGGGTCGCGTTCCTGGCCGTTGGCCCGATACCGCTCAAGGCGATACGCGG  >  NC_002947/791699‑791994
                                                                                                                                                    |                                                                                                                                                   
cAGCTTCAATTGCAGGATTTCGATCTGTGCGCCCAGCGGGAAGCCATAGGCGTTGTCATTCAGGTCGTGGTAGGCCATGCGCAAGCCATATTCGGCGTAGGCGCGGTCTTCACGGGTGCCCACGCCCAGTTGCCAGGTGCGCGAGTCAt                                                                                                                                                     <  5:102986/149‑1 (MQ=255)
cAGCTTCAATTGCAGGATTTCGATCTGTGCGCCCAGCGGGAAGCCATAGGCGTTGTCATTCAGGTCGTGGTAGGCCATGCGCAAGCCATATTCGGCGTAGGCGCGGTCTTCACGGGTGCCCACGCCCAGTTGCCAGGTGCGCGAGTCAt                                                                                                                                                     >  3:172200/1‑149 (MQ=255)
cAGCTTCAATTGCAGGATTTCGATCTGTCCGCCCAGCGGGAAGCCATAGGCGTTGTCATTCAGCGCGTGGTACGCCATGCGCAAGCCATATTCGGCGTAGGCGCGGTCTTGACGGGTGCCCACGCCCAGTTGCCAGGTGCGCGAGTCAt                                                                                                                                                     <  5:97849/149‑1 (MQ=255)
 aGCTTCAATTGCAGGATTTCGATCTGTGCGCCCAGCGGGAAGCCATAGGCGTTGTCATTCAGGTCGTGGTAGGCCATGCGCAAGCCATATTCGGCGTAGGCGCGGTCTTCACGGGTGCCCACGCCCAGTTGCCAGGTGCGCGAGTCATg                                                                                                                                                    <  5:236200/149‑1 (MQ=255)
  gCTTCAATTGCAGGATTTCGATCTGTGCGCCCAGCGGGAAGCCATAGGCGTTGTCATTCAGGTCGTGGTAGGCCATGCGCAAGCCATATTCGGCGTAGGCGCGGTCTTCACGGGTGCCCACGCCCAGTTGCCAGGTGCGCGAGTCATgg                                                                                                                                                   <  2:14411/149‑1 (MQ=255)
         ttGCAGGATTTCGATCTGTGCGCCCAGCGGGAAGCCATAGGCGTTGTCATTCAGGTCGTGGTAGGCCATGCGCAAGCCATATTCGGCGTAGGCGCGGTCTTCACGGGTGCCCACGCCCAGTTGCCAGGTGCGCGAGTCATGGCCATCtt                                                                                                                                            <  3:13106/149‑1 (MQ=255)
               gATTTCGATCTGTGCGCCCAGCGGGAAGCCATAGGCGTTGTCATTCAGGTCGTGGTAGGCCATGCGCAAGCCATATTCGGCGTAGGCGCGGTCTTCACGGGTGCCCACGCCCAGTTGCCAGGTGCGCGAGTCATGGCCATCTTCggg                                                                                                                                        >  1:242195/1‑147 (MQ=255)
                aTTTCGATCTGTGCGCCCAGCGGGAAGCCATAGGCGTTGTCATTCAGGTCGTGGTAGGCCATGCGCAAGCCATATTCGGCGTAGGCGCGGTCTTCACGGGTGCCCACGCCCAGTTGCCAGGTGCGCGAGTCATGGCCATCTTCGGGCAg                                                                                                                                     <  2:68099/149‑1 (MQ=255)
                     gATCTGTGCGCCCAGCGGGAAGCCATAGGCGTTGTCATTCAGGTCGTGGTAGGCCATGCGCACGCGTTATTCGGCGTAGGCGCGGTCTTCACGGGTGCCCACGCCCAGTTGACAGGTGCGCGAGTCATGGCCATCTTCGGGCAGGCCgg                                                                                                                                >  8:276274/1‑149 (MQ=255)
                     gATCTGTGCGCCCAGCGGGAAGCCATAGGCGTTGTCATTCAGGTCGTGGTAGGCCATGCGCAAGCCATATTCGGCGTAGGCGCGGTCTTCACGGGTGCCCACGCCCAGTTGCCAGGTGCGCGAGTCATGGCCATCTTCGGGCAGGCCgg                                                                                                                                >  1:39693/1‑149 (MQ=255)
                     gATCTGTGCGCCCAGCGGGAAGCCATAGGCGTTGTCATTCAGGTCGTGGTAGGCCATGCGCAAGCCATATTCGGCGTAGGCGCGGTCTTCACGGGTGCCCACGCCCAGTTGCCAGGTGCGCGAGTCATGGCCATCTTCGGGCAGGCCgg                                                                                                                                >  3:309765/1‑149 (MQ=255)
                          gtgCGCCCAGCGGGAAGCCATAGGCGTTGTCATTCAGGTCGTGGTAGGCCATGCGCAAGCCATATTCGGCGTAGGCGCGGTCTTCACGGGTGCCCACGCCCAGTTGCCAGGTGCGCGAGTCATg                                                                                                                                                    <  8:245219/124‑1 (MQ=255)
                          gtgCGCCCAGCGGGAAGCCATAGGCGTTGTCATTCAGGTCGTGGTAGGCCATGCGCAAGCCATATTCGGCGTAGGCGCGGTCTTCACGGGTGCCCACGCCCAGTTGCCAGGTGCGCGAGTCATg                                                                                                                                                    >  7:245219/1‑124 (MQ=255)
                           tgCGCCCAGCGGGAAGCCATAGGCGTTGTCATTCAGGTCGTGGTAGGCCATGCGCAAGCCATATTCGGCGTAGGCGCGGTCTTCACGGGTGCCCACGCCCAGTTGCCAGGTGCGCGAGTCATGGCCATCTTCGGGCAGGCCGGGGCGCt                                                                                                                          <  4:172200/149‑1 (MQ=255)
                                ccAGCGGGAAGCCATAGGCGTTGTCATTCAGGTCGTGGTAGGCCATGCGCAAGCCATATTCGGCGTAGGCGCGGTCTTCACGGGTGCCCACGCCCAGTTGCCAGGTGCGCGAGTCATgg                                                                                                                                                   <  2:234187/119‑1 (MQ=255)
                                ccAGCGGGAAGCCATAGGCGTTGTCATTCAGGTCGTGGTAGGCCATGCGCAAGCCATATTCGGCGTAGGCGCGGTCTTCACGGGTGCCCACGCCCAGTTGCCAGGTGCGCGAGTCATgg                                                                                                                                                   >  1:234187/1‑119 (MQ=255)
                                          gCCATAGGCGTTGTCATTCAGGTCGTGGTAGGCCATGCGCAAGCCATATTCGGCGTAGGCGCGGTCTTCACGGGTGCCCACGCCCAGTTGCCAGGTGCGCGAGTCATGGCCATCTTCGGGCAGGCCGGGGCGCTCGATCTGCAGGggcg                                                                                                           >  1:92620/1‑149 (MQ=255)
                                           ccATAGGCGTTGTCATTCAGGTCGTGGTAGGCCATGCGCAAGCCATATTCGGCGTAGGCGCGGTCTTCACGGGTGCCCACGCCCAGTTGCCAGGTGCGCGAGTCATGGCCATCTTCGGGCAGGCCGGGGCGCTCGATCTGCAGGggcgg                                                                                                          >  7:229745/1‑149 (MQ=255)
                                                ggCGTTGTCATTCAGGTCGTGGTAGGCCATGCGCAAGCCATATTCGGCGTAGGCGCGGTCTTCACGGGTGCCCACGCCCAGTTGCCAGGTGCGCGAGTCATGGCCATCTTCGGGCAGGCCGGGGCGCTCGATCTGCAGGggcggcggc                                                                                                      <  2:242195/148‑1 (MQ=255)
                                                 gCGTTGTCATTCAGGTCGTGGTAGGCCATGCGCAAGCCATATTCGGCGTAGGCGCGGTCTTCACGGGTGCCCACGCCCAGTTGCCAGGTGCGCGAGTCATg                                                                                                                                                    <  4:218023/101‑1 (MQ=255)
                                                 gCGTTGTCATTCAGGTCGTGGTAGGCCATGCGCAAGCCATATTCGGCGTAGGCGCGGTCTTCACGGGTGCCCACGCCCAGTTGCCAGGTGCGCGAGTCATg                                                                                                                                                    >  3:218023/1‑101 (MQ=255)
                                                   gTTGTCATTCAGGTCGTGGTAGGCCATGCGCAAGCCATATTCGGCGTAGGCGCGGTCTTCACGGGTGCCCACGCCCAGTTGCCAGGTGCGCGAGTCATGGCCATCTTCGGGCAGGCCGGGGCGCTCGATCTGCAGGGGCGGCGGCGGGt                                                                                                  >  3:154262/1‑149 (MQ=255)
                                                         aTTCAGGTCGTGGTAGGCCATGCGCAAGCCATATTCGGCGTAGGCGCGGTCTTCACGGGTGCCCACGCCCAGTTGCCAGGTGCGCGAGTCATGGCCATCTTCGGGCAGGCCGGGGCGCTCGATCTGCAGGGGCGGCGGCGGGTTGCTGt                                                                                            >  1:11171/1‑149 (MQ=255)
                                                         aTTCAGGTCGTGGTAGGCCATGCGCAAGCCATATTCGGCGTAGGCGCGGTCTTCACGGGTGCCCACGCCCAGTTGCCAGGGGCGCGAGTCATGGCCATCTTCGGCCAGGCCGGGGCGCTCGATCTGCAGGGGCTGCGGCGGGTTGCTGt                                                                                            <  7:276274/149‑1 (MQ=255)
                                                           tCAGGTCGTGGTAGGCCATGCGCAAGCCATATTCGGCGTAGGCGCGGTCTTCACGGGTGCCCACGCCCAGTTGCCAGGTGCGCGAGTCATGGCCATCTTCGGGCAGGCCGGGGCGCTCGATCTGCAGGGGCGGCGGCGGGTTGCTGtt                                                                                           <  8:229745/148‑1 (MQ=255)
                                                                     gTAGGCCATGCGCAAGCCATATTCGGCGTAGGCGCGGTCTTCACGGGTGCCCACGCCCAGTTGCCAGGTGCGCGAGTCATGGCCATCTTCGGGCAGGCCGGGGCGCTCGATCTGCAGGGGCGGCGGCGGGTTGCTGTTGATCGCCCgca                                                                                <  4:309765/149‑1 (MQ=255)
                                                                     gTAGGCCATGCGCAAGCCATATTCGGCGTAGGCGCGGTCTTCACGGGTGCCCACGCCCAGTTGCCAGGTGCGCGAGTCAAGGCCATCTTCGGGCAGGCCGGGGCGCTCGATCTGCAGGGGCGGCGGCGGGTTGCTGTTGATCGCCCg                                                                                  >  8:184456/1‑147 (MQ=255)
                                                                     gTAGGCCATGCGCAAGCCATATTCGGCGTAGGCGCGGTCTTCACGGGTGCCCACGCCCAGATGCCAGGTGCGCGAGTCATGGCCATCTTCGGGCAGGCCGGGGAGCTCGATCTGCAGGGGCGGCGGCGGGTTGCTGTTGATCGCCCg                                                                                  >  1:105374/1‑147 (MQ=255)
                                                                         gCCATGCGCAAGCCATATTCGGCGTAGGCGCGGTCTTCACGGGTGCCCACGCCCAGTTGCCAGGTGCGCGAGTCATGGCCATCTTCGGGCAGGCCGGGGCGCTCGATCTGCAGGGGCGGCGGCGGGTTGCTGTTGATCGCCCGCAGCaa                                                                            >  1:335094/1‑149 (MQ=255)
                                                                               cgcAAGCCATATTCGGCGTAGGCGCGGTCTTCACGGGTGCCCACGCCCAGTTGCCAGGTGCGCGAGTCATGGCCATCTTCGGGCAGGCCGGGGCGCTCGATCTGCAGGGGCGGCGGCggg                                                                                                   >  8:27585/1‑120 (MQ=255)
                                                                               cgcAAGCCATATTCGGCGTAGGCGCGGTCTTCACGGGTGCCCACGCCCAGTTGCCAGGTGCGCGAGTCATGGCCATCTTCGGGCAGGCCGGGGCGCTCGATCTGCAGGGGCGGCGGCggg                                                                                                   <  7:27585/120‑1 (MQ=255)
                                                                                gcAAGCCATATTCGGCGTAGGCGCGGTCTTCACGGGTGCCCACGCCCAGTTGCCAGGTGCGCGAGTCATGGCCATCTTCGGGCAGGCCGGGGCGCTCGATCTGCAGGGGCGGCGGCGGGTTGCTGTTGATCGCCCGCAGCAACTCGAAg                                                                     >  7:241859/1‑149 (MQ=255)
                                                                                gcAAGCCATATTCGGCGTAGGCGCGGTCTTCACGGGTGCCCACGCCCAGTTGCCAGGTGCGCGAGTCATGGCCATCTTCGGGCAGGCCGGGGCGCTCGATCTGCAGGGGCGGCGGCGGGTTGCTGTTGATCGCCCGCAGCAACTCGAAg                                                                     <  2:39693/149‑1 (MQ=255)
                                                                                            cGGCGTAGGCGCGGTCTTCACGGGTGCCCACGCCCAGTTGCCAGGTGCGCGAGTCATGGCCATCTTCGGGCAGGCCGGGGCGCTCGATCTGCAGGGGCGGCGGCGGGTTGCTGTTGATCGCCCGCAGCAACTCGAAGCTGCGTTTGGc                                                          <  4:154262/148‑1 (MQ=255)
                                                                                               cGTAGGCGCGGTCTTCACGGGTGCCCACGCCCAGTTGCCAGGTGCGCGAGTCATGGCCATCTTCGGGCAGGCCGGGGCGCTCGATCTGCAGGGGCGGCGGCGGGTTGCTGTTGATCGCCCGCAGCAACTCGAAGCTGCGTTTGGCTTGc                                                      <  2:11171/149‑1 (MQ=255)
                                                                                                     cgcgGTCTTCACGGGTGCCCACGCCCAGTTGCCAGGTGCGCGAGTCATGGCCATCTTCGGGCAGGCCGGGGCGCTCGATCTGCAgg                                                                                                               <  4:120800/86‑1 (MQ=255)
                                                                                                     cgcgGTCTTCACGGGTGCCCACGCCCAGTTGCCAGGTGCGCGAGTCATGGCCATCTTCGGGCAGGCCGGGGCGCTCGATCTGCAgg                                                                                                               >  3:120800/1‑86 (MQ=255)
                                                                                                      gcgGTCTTCACGGGTGCCCACGCCCAGTTGCCAGGTGCGCGAGTCATGGCCATCTTCGGGCAGGCCGGGGCGCTCGATCTGCAGGGGCGGCGGCGGGTTGCTGTTGATCGCCCGCAGCAACTCGAAGCTGCGTTTGGCTTGCCCTGGGt                                               >  1:16957/1‑149 (MQ=255)
                                                                                                             tCACGGGTGCCCACGCCCAGTTGCCAGGTGCGCGAGTCATGGCCATCTTCGGGCAGGCCGGGGCGCTCGATCTGCAGGGGCGGCGGCGGGTTGCTGTTGATCGCCCGCAGCAACTCGAAGCTGCGTTTGGCTTGCCCTGGGTCGCGTTc                                        >  7:203152/1‑149 (MQ=255)
                                                                                                                        cACGCCCAGTTGCCAGGTGCGCGAGTCACGGCCATCTTCGGGCAGGCCGGGGCGCTCGATCTGCAGGGGCGGCGGCGGGTTGCTGTTGATCGCCCGCAGCAACTCGAAGCTGCGTTTGGCTTGCCCTGGGTCGCGTTCCTGGCCg                                 >  4:231327/1‑145 (MQ=255)
                                                                                                                        cACGCCCAGTTGCCAGGTGCGCGAGTCACGGCCATCTTCGGGCAGGCCGGGGCGCTCGATCTGCAGGGGCGGCGGCGGGTTGCTGTTGATCGCCCGCAGCAACTCGAAGCTGCGTTTGGCTTGCCCTGGGTCGCGTTCCTGGCCg                                 <  3:231327/145‑1 (MQ=255)
                                                                                                                        cACGCCCAGTTGCCAGGTGCGCGAGTCACGGCCATCTTCGGGCAGGCCGGGGCGCTCGATCTGCAGGGGCGGCGGCGGGTTGCTGTTGATCGCCCGCAGCAACTCGAAGCTGCGTTTGGCTTGCCCTGGGTCGCGTTCCTGGCCg                                 <  3:74417/145‑1 (MQ=255)
                                                                                                                        cACGCCCAGTTGCCAGGTGCGCGAGTCACGGCCATCTTCGGGCAGGCCGGGGCGCTCGATCTGCAGGGGCGGCGGCGGGTTGCTGTTGATCGCCCGCAGCAACTCGAAGCTGCGTTTGGCTTGCCCTGGGTCGCGTTCCTGGCCg                                 >  4:74417/1‑145 (MQ=255)
                                                                                                                         aCGCCCAGTTGCCAGGTGCGCGAGTCATGGCCATCTTCGGGCAGGCCGGGGCGCTCGATCTGCAGGGGCGGCGGCGGGTTGCTGTTGATCGCCCgca                                                                                <  6:63059/97‑1 (MQ=255)
                                                                                                                         aCGCCCAGTTGCCAGGTGCGCGAGTCATGGCCATCTTCGGGCAGGCCGGGGCGCTCGATCTGCAGGGGCGGCGGCGGGTTGCTGTTGATCGCCCgca                                                                                >  5:63059/1‑97 (MQ=255)
                                                                                                                             ccAGTTGCCAGGTGCGCGAGTCATGGCCATCTTCGGGCAGGCCGGGGCGCTCGATCTGCAGGGGCGGCGGCGGGTTGCTGt                                                                                            <  4:20181/81‑1 (MQ=255)
                                                                                                                             ccAGTTGCCAGGTGCGCGAGTCATGGCCATCTTCGGGCAGGCCGGGGCGCTCGATCTGCAGGGGCGGCGGCGGGTTGCTGt                                                                                            >  3:20181/1‑81 (MQ=255)
                                                                                                                             ccAGTTGCCAGGTGCGCGAGTCATGGCCATCTTCGGGCAGGCCGGGGCGCTCGATCTGCAGGGGCGGCGGCGGGTTGCTGTTGATCGCCCGCAGCAACTCGAAGCTGCGTTTGGCTTGCCCTGGGTCGCGTTCCTGGCCGTTGGCCCGa                        >  2:339393/1‑149 (MQ=255)
                                                                                                                               aGTTGCCAGGTGCGCGAGTCATGGCCATCTTCGGGCTGGCCGGGGCGCTCGATCTGCAGGGGCGGCGGCGGGTTGCTGTTGATCGCCCGCAGCAACTCGAAGCTGCGTTTGGCTTGCCCTGGGTCGCGTTCCTGGCCGTTGGCCCGATa                      <  2:105374/149‑1 (MQ=255)
                                                                                                                                       ggTGCGCGAGTCATGGCCATCTTCGGGCAGGCCGGGGCGCTCGATCTGCAGGGGCGGCGGCGGGTTGCTGTTGATCGCCCGCAGCAACTCGAAGCTGCGTTTGGCTTGCCCTGGGTCGCGTTCCTGGCCGTTGGCCCGATACCGCTc                >  7:59024/1‑147 (MQ=255)
                                                                                                                                         tGCGCGAGTCATGGCCATCTTCGGGCAGGCCGGGGCGCTCGATCTGCAGGGGCGGCGGCGGGTTGCTGTTGATCGCCCGCAGCAACTCGAAGCTGCGTTTGGCTTGCCCTGGGTcgc                                            <  2:236295/117‑1 (MQ=255)
                                                                                                                                         tGCGCGAGTCATGGCCATCTTCGGGCAGGCCGGGACGCTCGATCTGCAGGGGCGGCGGCGGGTTGCTGTTGATCGCCCGCAGCAACTCGAAGCTGCGTTTGGCTTGCCCTGGGTcgc                                            >  1:236295/1‑117 (MQ=255)
                                                                                                                                            gcgAGTCATGGCCATCTTCGGGCAGGCCGGGGCGCTCGATCTGCAGGGGCGGCGGCGGGTTGCTGTTGATCGCCCGCAGCAACTCGAAGCTGCGTTTGGCTTGCCCTGGGTCGCGTTCCTGGCCGTTGGCCCGATACCGCTCAAGGCGa         >  2:351479/1‑149 (MQ=255)
                                                                                                                                                   aTGGCCATCTTCGGGCAGGCCGGGGCGCTCGATCTGGAGGGGCGGCGGCGGGTTGCTGTTGATCGCCCGCAGCAACTCGAAGCTGCGTTTGGCTTGCCCTGGGTCGCGTTCCTGGCCGTTGGCCCGATACCGCTCAAGGCGATAcgcg   <  8:241859/148‑1 (MQ=255)
                                                                                                                                                   aTGGCCATCTTCGGGCAGGCCGGGGCGCTCGATCTGCAGGGGCGGCGGCGGGTTGCTGTTGATCGCCCGCAGCAACTCGAAGCTGCGttt                                                             <  7:299334/90‑1 (MQ=255)
                                                                                                                                                   aTGGCCATCTTCGGGCAGGCCGGGGCGCTCGATCTGCAGGGGCGGCGGCGGGTTGCTGTTGATCGCCCGCAGCAACTCGAAGCTGCGttt                                                             >  8:299334/1‑90 (MQ=255)
                                                                                                                                                   aTGGCCATCTTCGGGCAGGCCGGGGCGCTCGATCTGCAGGGGCGGCGGCGGGTTGCTGTTGATCGCCCGCAGCAACTCGAAGCTGCGTTTGGCTTGCCCTGGGTCGCGTTCCTGGCCGTTGGCCCGATACCGCTCAAGGCGATACGCgg  >  6:78229/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CAGCTTCAATTGCAGGATTTCGATCTGTGCGCCCAGCGGGAAGCCATAGGCGTTGTCATTCAGGTCGTGGTAGGCCATGCGCAAGCCATATTCGGCGTAGGCGCGGTCTTCACGGGTGCCCACGCCCAGTTGCCAGGTGCGCGAGTCATGGCCATCTTCGGGCAGGCCGGGGCGCTCGATCTGCAGGGGCGGCGGCGGGTTGCTGTTGATCGCCCGCAGCAACTCGAAGCTGCGTTTGGCTTGCCCTGGGTCGCGTTCCTGGCCGTTGGCCCGATACCGCTCAAGGCGATACGCGG  >  NC_002947/791699‑791994

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑