Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 805,137 G→T 6.7% S731S (TCC→TCA) ‡ PP_0693 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947805,1370GT 6.7% 239.3 / 5.0 74S731S (TCC→TCA) ‡PP_0693hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (34/35);  new base T (2/3);  total (36/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.47e-01

CTTGTTGTCCTGATCGGCACTCTGGGTCAGGTCGAAG‑ACCGACAGGGTTTCGCCTTCTTCGTACTGGCCGGCACTGTGGATAATCTGGTCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGTTGCGCAGGGTTCGGGCGATGTCGGTGACCGCAGCGCGGCGTGCCTTGGCCAAGGTCAGGGCTTCGTCGGT  >  NC_002947/804990‑805283
                                                                                                                                                    |                                                                                                                                                  
cTTGTTGTCCTGATCGGCACTCTGGGTCAGGTCGAAG‑ACCGACAGGGTTTCGCCTTCTTCGTACTGGCCGGCACTGTGGATAATCTGGTCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTg                                                                                                                                                    >  6:189736/1‑148 (MQ=255)
    ttgtCCTGATCGGCACTCTGGGTCAGGTCGAAG‑ACCGACAGGGTTTCGCCTTCTTCGTACTGGCCGGCACTGTGGATAATCTGGTCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAAc                                                                                                                                               >  4:131579/1‑149 (MQ=255)
       tCCTGATCGGGACTCTGGGTCAGGTCGAAG‑ACCGACAGGGTTTCGCCTTCTTCGTACTGGCCGGCACTGTGGATAATCTGGTCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCt                                                                                                                                             <  6:215141/148‑1 (MQ=255)
       tCCTGATCGGCACTCTGGGTCAGGTCGAAG‑ACCGACAGGGTTTCGCCTTCTTCGTACTGGCCGGCACTGTGGATAATCTGGTCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTg                                                                                                                                            >  6:33725/1‑149 (MQ=255)
         cTGATCGGCACTCTGGGTCAGGTCGAAG‑ACCGACAGGGTTTCGCCTTCTTCGTACTGGCCGGCACTGTGGATAATCTGGTCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTgg                                                                                                                                           <  2:215068/148‑1 (MQ=255)
         cTGATCGGCACTCTGGGTCAGGTCGAAG‑ACCGACAGGGTTTCGCCTTCTTCGTACTGGCCGGCACTGTGGATAATCTGGTCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTgg                                                                                                                                           <  3:131579/148‑1 (MQ=255)
         cTGATCGGCACTCTGGGTCAGGTCGAAG‑ACCGACAGGGTTTCGCCTTCTTCGTACTGGCCGGCACTGTGGATAATCTGGTCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTgg                                                                                                                                           <  6:176514/148‑1 (MQ=255)
         cTGATCGGCACTCTGGGTCAGGTCGAAG‑ACCGACAGGGTTTCGCCTTCTTCGTACTGGCCGGCACTGTGGATAATCTGGTCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGgc                                                                                                                                          <  3:65791/149‑1 (MQ=255)
          tGATCGGCACTCTGGGTCAGGTCGAAG‑ACCGACAGGGTTTCGCCTTCTTCGTACTGGCCGGCACTGTGGATAATCTGGTCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTTCCAACCTgga                                                                                                                                          >  1:313494/1‑147 (MQ=255)
          tGATCGGCACTCTGGGTCAGGTCGAAG‑ACCGACAGGGTTTCGCCTTCTTCGTACTGGCCGGCACTGTGGATAATCTGGTCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGgc                                                                                                                                          >  2:314174/1‑148 (MQ=255)
            aTCGGCACTCTGGGTCAGGTCGAAGAACCGACAGGGTTTCGCCTTCTTCGTACTGGCCGGCACTGTGGATAATCTGGTCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGATACCTGgcgc                                                                                                                                        >  1:3568/1‑149 (MQ=255)
            aTCGGCACTCTGGGTCAGGTCGAAG‑ACCGACAGGGTTTCGCCTTCTTCGTACTGGCCGGCACTGTGGATAATCTGGTCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTTCCAACCTggaaa                                                                                                                                        <  5:222242/148‑4 (MQ=255)
            aTCGGCACTCTGGGTCAGGTCGAAG‑ACCGACAGGGTTTCGCCTTCTTCGTACTGGCCGGCACTGTGGATAATCTGGTCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTTCCAACCTggaaa                                                                                                                                        <  2:31949/148‑4 (MQ=255)
            aTCGGCACTCTGGGTCAGGTCGAAG‑ACCGACAGGGTTTCGCCTTCTTCGTACTGGCCGGCACTGTGGATAATCTGGTCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTTCCAACCTggaaa                                                                                                                                        <  2:313494/148‑4 (MQ=255)
            aTCGGCACTCTGGGTCAGGTCGAAG‑ACCGACAGGGTTTCGCCTTCTTCGTACTGGCCGGCACTGTGGATAATCTGGTCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGgcgc                                                                                                                                        <  6:90061/148‑1 (MQ=255)
               ggCACTCTGGGTCAGGTCGAAG‑ACCGACAGGGTTTCGCCTTCTTCGTACTGGCCGGCACTGTGGATAATCTGGTCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCttgt                                                                                                                                    <  1:51851/149‑1 (MQ=255)
                gCACTCTGGGTCAGGTCGAAG‑CCCGACAGGGTTTCGCCTTCTTCGTACTGGCCGGCACTGTGGATAATCTGGTCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCttgtt                                                                                                                                   >  6:85245/1‑149 (MQ=255)
                 cACTCTGGGTCAGGTCGAAG‑ACCGACAGGGTTTCGCCTTCTTCGTACTGGCCGGCACTGTGGATAATCTGGTCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCttgttg                                                                                                                                  >  8:161401/1‑149 (MQ=255)
                   ctctGGGTGAGGTCTAAG‑ACCGACAGGGGTTCGCCTTCTTCGTACTGGCCGGCACTGGGGATAATCTGGTCGATGTGCTTGAGCGCTTCCTTGTTCGGCTCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGAt                                                                                                                                <  2:289036/149‑1 (MQ=255)
                    tctGGGTCAGGTCGAAG‑ACCGACAGGGTTTCGCCTTCTTCGTACTGGCCGGCACTGTGGATAATCTGGTCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGAtc                                                                                                                               >  6:341237/1‑149 (MQ=255)
                        ggTCAGGTCGAAG‑ACCGACAGGGTTTCGCCTTCTTCGTACTGGCCGGCACTGTGGATAATCTGGTCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTcgc                                                                                                                           >  8:227281/1‑149 (MQ=255)
                               tCGAAG‑ACCGACAGGGTTTCGCCTTCTTCGTACTGGCCGGCACTGTGGATAATCTGGTCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGaa                                                                                                                    <  4:106417/149‑1 (MQ=255)
                                 gAAG‑ACCGACAGGGTTTCGCCTTCTTCGTACTGGCCGGCACGGTGGATAATCTGGTCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCgg                                                                                                                         >  2:44609/1‑142 (MQ=255)
                                 gAAG‑ACCGACAGGGTTTCGCCTTCTTCGTACTGGCCGGCACGGTGGATAATCTGGTCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCgg                                                                                                                         <  1:44609/142‑1 (MQ=255)
                                      aCCGACAGGGTTTCGCCTTCTTCGTACTGGCCGGCACTGTGGATAATCTGGTCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGc                                                                                                              <  5:341237/149‑1 (MQ=255)
                                           cAGGGTTTCGCCTTCTTCGTACTGGCCGGCACTGTGGATAATCTGGTCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCt                                                                                                         <  3:137494/149‑1 (MQ=255)
                                               gTTTCGCCTTCTTCGTACTGGCCGGCACTGTGGATAATCTGGTCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTg                                                                                                     <  5:85245/149‑1 (MQ=255)
                                                    gCCTTCTTCGTACTGGCCGGCACTGTGGATAATCTGGTCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTc                                                                                                  >  4:51522/1‑147 (MQ=255)
                                                     ccTTCTTCGTACTGGCCGGCACTGTGGATAATCTGGTCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAg                                                                                               >  4:100008/1‑149 (MQ=255)
                                                      cttcttCGTACTGGCCGGCACTGTGGATAATCTGGTCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAgg                                                                                              <  3:100008/149‑1 (MQ=255)
                                                          ttcGTACTGGCCGGCACTGTGGATAATCTGGTCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGt                                                                                          >  6:100324/1‑149 (MQ=255)
                                                           tcGTACTGGCCGGCACTGTGGATAATCTGGTCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAAc                                                                                                                   <  8:153601/123‑1 (MQ=255)
                                                            cGTACTGGCCGGCACTGTGGATAATCTGGTCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTc                                                                                         <  3:176933/148‑1 (MQ=255)
                                                             gTACTGGCCGGCACTGTGGATAATGTGGTCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCg                                                                                        <  5:304229/148‑1 (MQ=255)
                                                              tACTGGCCGGCACTGTGGATAATCTGGTCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTa                                                                                      <  5:33725/149‑1 (MQ=255)
                                                                   gCCGGCACTGTGGATAATCTGGTCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGc                                                                                 >  8:77140/1‑149 (MQ=255)
                                                                          cTGTGGATAATCTGGTCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGAt                                                                          >  8:22920/1‑149 (MQ=255)
                                                                                 tAATCTGGTCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGTTgcgc                                                                   <  7:161401/149‑1 (MQ=255)
                                                                                               tgCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGTTGCGCAGGGTTCGGGCGa                                                      <  5:189736/148‑1 (MQ=255)
                                                                                               tgCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGTTGCGCAGGGTTCGGGCGAt                                                     <  1:314174/149‑1 (MQ=255)
                                                                                               tgCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGTTGCGCAGGGTTCGGGCGAt                                                     <  7:22920/149‑1 (MQ=255)
                                                                                               tgCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGTTGCGCAGGGTTCGGGCGAt                                                     <  7:227281/149‑1 (MQ=255)
                                                                                                gCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCt                                                                                                   >  7:101751/1‑102 (MQ=255)
                                                                                                gCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCt                                                                                                   <  8:101751/102‑1 (MQ=255)
                                                                                                       cgcTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGTTGCGCAGGGTTCGGGCGATGTCGGTGa                                             >  8:193769/1‑149 (MQ=255)
                                                                                                          ttCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAggg                                                                                                               >  8:189237/1‑80 (MQ=255)
                                                                                                          ttCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAggg                                                                                                               <  7:189237/80‑1 (MQ=255)
                                                                                                          ttCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGtt                                                                       >  4:135898/1‑120 (MQ=255)
                                                                                                          ttCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGtt                                                                       <  3:135898/120‑1 (MQ=255)
                                                                                                          ttCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGTTGCGCa                                                                  <  2:125561/125‑1 (MQ=255)
                                                                                                          ttCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGTTGCGCa                                                                  >  1:125561/1‑125 (MQ=255)
                                                                                                          ttCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGTTGCGCAGGGTTCGGGCGATGTCGGt                                               <  1:181475/144‑1 (MQ=255)
                                                                                                          ttCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGTTGCGCAGGGTTCGGGCGATGTCGGt                                               >  2:181475/1‑144 (MQ=255)
                                                                                                          ttCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGTTGCGCAGGGTTCGGGCGATGTCGGTGACCg                                          >  1:246545/1‑149 (MQ=255)
                                                                                                          ttCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGTTGCGCAGGGTTCGGGCGATGTCGGTGACCg                                          >  2:289159/1‑149 (MQ=255)
                                                                                                          ttCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGTTGCGCAGGGTTCGGGCGATGTCGGTGACCg                                          >  2:292490/1‑149 (MQ=255)
                                                                                                             cTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCGAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGTTGCGCAGGGTTCGGGCGATGTCGGTGACCGCAg                                       <  3:51522/149‑1 (MQ=255)
                                                                                                               tGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGTTGCGCAGGGTTCGGGCGATGTCGGTGACCGCAgtg                                     >  4:353998/1‑147 (MQ=255)
                                                                                                                    ggCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGTTGCGCAGGGTTCGGGCGATGTCGGTGACCGCAGTgcggc                                 <  3:353998/148‑1 (MQ=255)
                                                                                                                    ggCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGTTGCGCAGGGTTCGGGCGATGTCGGTGACCGCAGCgcggcg                                >  2:257233/1‑149 (MQ=255)
                                                                                                                           gTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGTTGCGCAGGGTTCGGGCGATGTCGGTGACCGCAGCGCGGCGTGCCTTg                         <  7:77140/149‑1 (MQ=255)
                                                                                                                              ccACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGTTGCGCAGGGTTCGGGCGATGTCGGTGACCGCAGCGCGGCGTGCCTTGGc                       >  2:259990/1‑148 (MQ=255)
                                                                                                                               cACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGTTGCGCAGGGTTCGGGCGATGTCGGTGACCGCAGCGCGGCGTGCCTTGGcc                      >  2:2263/1‑148 (MQ=255)
                                                                                                                               cACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGTTGCGCAGGGTTCGGGCGATGTCGATGACCGCAGCGCGGCGTGCCTTGGcc                      >  2:18348/1‑148 (MQ=255)
                                                                                                                                 cgcgGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGTTGCGCAGGGTTCGGGCGATGTCGGTGACCGCAGCGCGGCGTGCCTTGGCCAAg                   <  8:252894/149‑1 (MQ=255)
                                                                                                                                     gAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCTGATGTTGCGCAGGGTTCGGGCGATGTCGGTGACCGCAGCGCGGCGTGCCTTGGCCAAGGTCa               <  1:292490/149‑1 (MQ=255)
                                                                                                                                         agcgccAGGTTTCCAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGTTGCGCAGGGTTCGGGCGATGTCGGTGACCGCAGCGCGGCGTGCCTTGGCCAAGGTCAGGGc           >  2:79177/5‑149 (MQ=255)
                                                                                                                                           ttCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGTTGCGCAGGGTTCGGGCGATGTCGGTGACCGCAGCGCGGCGTGCCTTGGCCAAGGTCAGGGCtt         >  1:324352/1‑149 (MQ=255)
                                                                                                                                             ccAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGTTGCGCAGGGTTCGGGCGATGTCGGTGACCGCAGCGCGGCGTGCCTTGGCCAAGGTCAGGGCTtcg       >  3:62013/1‑149 (MQ=255)
                                                                                                                                              cAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGTTGCGCAGGGTTCGGGCGATGTCGGTGACCGCAGCGCGGCGTGCCTTGGCCAAGGTCAGGGCTtcgt      >  5:269857/1‑149 (MQ=255)
                                                                                                                                              cAGGATGGAAACCTGGCGCTTGTTGATATCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGAAGTCGCTGCGGATGAAGCGCAGGGTTCGCGCGATGTCGGTGACCGCAGCGCGGCGTGCCTTGGCCAAGGTCAGGCCTtcgt      >  8:253799/1‑149 (MQ=255)
                                                                                                                                               aGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGTTGCGCAGGGTTCGGGCGATGTCGGTGACCGCAGCGCGGCGTGCCTTGGCCAAGGTCAGGGCTtcgtc     <  4:62013/149‑1 (MQ=255)
                                                                                                                                                ggTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGTTGCGCAGGGTTCGGGCGATGTCGGAGACCGCAGCGCGGCGTGCCTTGGCCAAGGTCAGGGCTtcgtcg    >  4:276082/1‑149 (MQ=255)
                                                                                                                                                 gTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGTTGCGCAGGGTTCGGGCGATGTCGGTGACCGCAGCGCGGCGTGCCTTGGCCAAGGTCAGGGCTTCGTCgg   >  4:155653/1‑149 (MQ=255)
                                                                                                                                                  ttGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGTTGCGCAGGGGTCGGGCGATGTCGGTGACCGCAGCGCGGCGTGCCTTGGCCAAGGTCAGGGCTTCGTCGGt  <  2:3568/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CTTGTTGTCCTGATCGGCACTCTGGGTCAGGTCGAAG‑ACCGACAGGGTTTCGCCTTCTTCGTACTGGCCGGCACTGTGGATAATCTGGTCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGTTGCGCAGGGTTCGGGCGATGTCGGTGACCGCAGCGCGGCGTGCCTTGGCCAAGGTCAGGGCTTCGTCGGT  >  NC_002947/804990‑805283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑