Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	806,084	G→C	7.1%	R416G (CGC→GGC)	PP_0693 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	806,084	0	G	C	7.1%	213.1 / 4.9	67	R416G (CGC→GGC)	PP_0693	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (37/25);  new base C (3/2);  total (40/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

CCAGGCCTGGGGCCTCGAAGCGTTCGCCCTTGAAGCCGTCGAGCACCGCTTCCAGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGCGTTTTTCCAGGTCGCGTACGCGCTCGTCGAGGTCTTCGGCACTGAACTGCCGTGACTGG  >  NC_002947/805937‑806232                                                                                                                                                    |                                                                                                                                                     ccAGGCCTGGGGCCTCGAAGCGTTCGCCCTTGAAGCCGTCGAGCACCGCTTCCAGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCgg                                                                                                                                                     <  8:85045/149‑1 (MQ=255) ccAGGCCTGGGGCCTCGAAGCGTTCGCCCTTGAAGCCGTCGAGCACCGCTTCCAGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCgg                                                                                                                                                     >  6:183711/1‑149 (MQ=255) ccAGGCCTGGGGCCTCGAAGCGTTCGCCCTTGAAGCCGTCGAGCACCGCTTCCAGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCgg                                                                                                                                                     >  3:276946/1‑149 (MQ=255)   aGGCCTGGGGCCTCGAAGCGTTCGCCCTTGAAGCCGTCGAGCACCGCTTCCAGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTc                                                                                                                                                   <  5:99978/149‑1 (MQ=255)     gCCTGGGGCCTCGAAGCGTTCGCCCTTGAAGCCGTCGAGCACCGCTTCCAGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGa                                                                                                                                                 >  3:268941/1‑149 (MQ=255)       cTGGGGCCTCGAAGCGTTCGCCCTTGAAGCCGTCGAGCACCGCTTCCAGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACg                                                                                                                                               >  6:54115/1‑149 (MQ=255)       cTGGGGCCTCGAAGCGTTCGCCCTTGAAGCCGTCGAGCACCGCTTCCAGCGATTTTACACACAGGTCGCTGTAGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACg                                                                                                                                               >  7:186994/1‑149 (MQ=255)             ccTCGAAGCGTTCGCCCTTGAAGCCGTCGAGCACCGCTTCCAGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCctgc                                                                                                                                         >  8:253482/1‑149 (MQ=255)                cGAAGCGTTCGCCCTTGAAGCCGTCGAGCACCGCTTCCAGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGGCGTTGAACAGACGCATCAGGAGGTCGACGTCCtgctgc                                                                                                                                      >  8:2716/1‑149 (MQ=255)                  aaGCGTTCGCCCTTGAAGCCGTCGAGCACCGCTTCCAGCGATTTTACCAACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCAGCAGGACGTCGACCGCCTGATGCgt                                                                                                                                    <  4:79181/149‑2 (MQ=255)                     cGTTCGCCCTTGAAGCCGTCGAGCACCGCTTCCAGCGATTTTACCCACAGGTCGCTGTCGTCCATCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGaa                                                                                                                                 <  8:141537/149‑1 (MQ=255)                     cGTTCGCCCTTGAAGCCGTCGAGCACCGCTTCCAGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGaa                                                                                                                                 <  5:229267/149‑1 (MQ=255)                       ttCGCCCTTGAAGCCGTCGAGCACCGCTTCCAGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAAct                                                                                                                               >  8:238484/1‑149 (MQ=255)                            ccaagAAGCCGTCGAGCACCGCATCCAGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGAGAACTCCCAGCGGCAGGCTGAACAGCGAGCCGTAGAACAGACGCATCAGGCGGTCGACGTCCAGCTGCTAGAACTCTTcg                                                                                                                          >  2:331766/5‑149 (MQ=255)                            ccTTGAAGCCGTCGAGCACCGCTTCCAGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTcg                                                                                                                          >  6:351769/1‑149 (MQ=255)                            ccTTGAAGCCGTCGAGCACCGCTTCCAGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTcg                                                                                                                          <  5:54115/149‑1 (MQ=255)                              ttGAAGCCGTCGAGCACCGCTTCCAGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTc                                                                                                                           <  3:315103/146‑1 (MQ=255)                              ttGAAGCCGTCGAGCACCGCTTCCAGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTc                                                                                                                           >  4:315103/1‑146 (MQ=255)                                  aGCCGTCGAGCACCGCTTCCAGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAgg                                                                                                                    >  8:151553/1‑149 (MQ=255)                                     cGTCGAGCACCGCTTCCAGCGATTTTACGCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCgg                                                                                                                 >  1:207745/1‑149 (MQ=255)                                      gTCGAGCACCGCTTCCAGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCggg                                                                                                                >  8:267343/1‑149 (MQ=255)                                      gTCGAGCACCGCTTCCAGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCggg                                                                                                                <  8:186994/149‑1 (MQ=255)                                          aGCACCGCTTCCAGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATa                                                                                                            <  5:183711/149‑1 (MQ=255)                                                 cTTCCAGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCtgttg                                                                                                     >  5:118348/1‑149 (MQ=255)                                                    ccAGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCtgttgtt                                                                                                   <  4:276946/148‑1 (MQ=255)                                                    ccAGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCtgttgt                                                                                                    <  7:151553/147‑1 (MQ=255)                                                     cAGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCtgttgttgt                                                                                                 >  1:294586/1‑149 (MQ=255)                                                     cAGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCtgttgttgt                                                                                                 >  6:282451/1‑149 (MQ=255)                                                     cAGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCtgttgttgt                                                                                                 <  6:118348/149‑1 (MQ=255)                                                      aGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGGtgttgttgt                                                                                                 >  4:22866/1‑148 (MQ=255)                                                       gCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCtgttgttgt                                                                                                 >  8:320333/1‑147 (MQ=255)                                                                cccACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATcc                                                                                      <  2:294586/149‑1 (MQ=255)                                                                          gCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAgcggggcc                                                                             <  2:207745/148‑7 (MQ=255)                                                                            tGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTg                                                                                          >  7:132624/1‑133 (MQ=255)                                                                            tGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTg                                                                                          <  8:132624/133‑1 (MQ=255)                                                                                      cTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGAGGTCCTGCTGCGAGAACGCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCttaa                                                                <  7:238484/149‑3 (MQ=255)                                                                                         gATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAg                                                               <  5:282451/147‑1 (MQ=255)                                                                                         gATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAg                                                               <  4:268941/147‑1 (MQ=255)                                                                                              cgcgATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCtgt                                                                                                       <  6:102113/102‑1 (MQ=255)                                                                                              cgcgATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCtgt                                                                                                       >  5:102113/1‑102 (MQ=255)                                                                                               gcgATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGCGttttt                                                       <  5:351769/149‑1 (MQ=255)                                                                                                 gATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGAGTGATCCAGCTGCTGCTTGACCTGCTTCAGGCGTTTTTc                                                      >  2:302911/1‑148 (MQ=255)                                                                                                   tctcCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGCGTTTTTc                                                      >  7:305951/1‑146 (MQ=255)                                                                                                   tctcCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGCGTTTTTCCa                                                    <  8:305951/148‑1 (MQ=255)                                                                                                    ctcCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGCGTTTTTCCAgg                                                  >  3:67419/1‑149 (MQ=255)                                                                                                     tcCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGCGTTTTTc                                                      <  8:53121/144‑1 (MQ=255)                                                                                                     tcCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGCGTTTTTc                                                      >  7:53121/1‑144 (MQ=255)                                                                                                      cccAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCGGCTGCTGCTTGACCTGCTTCAGGCGTTTTTCCAGGTc                                                >  8:161873/1‑149 (MQ=255)                                                                                                       ccAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCCGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCAAGCTGCTGCTTGACCTGCTTCAGGCGTTTTTCCAGGTcg                                               >  1:262038/1‑149 (MQ=255)                                                                                                        cAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGCGTTTTTCCAGGTcgc                                              <  7:320333/149‑1 (MQ=255)                                                                                                        cAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGCGTTTTTCCAGGTcgc                                              >  3:175341/1‑149 (MQ=255)                                                                                                                      aCAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGCGTTTTTCCGGGTc                                                <  7:45070/133‑1 (MQ=255)                                                                                                                      aCAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGCGTTTTTCCGGGTc                                                >  8:45070/1‑133 (MQ=255)                                                                                                                       cAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGCGTTTTTCCAGGTCGCGTACGCGCtcgtc                                 >  3:361123/1‑147 (MQ=255)                                                                                                                                 ttGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGCGTTTTTCCAGGTCGCGTACGCGCTCGTCGAGGTCTTCgg                      >  5:360494/1‑148 (MQ=255)                                                                                                                                   gAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGCGTTTTTCCAGGTCGCGTACGCGCTCGTCGAGGTCTTCGGCAc                   >  8:345014/1‑149 (MQ=255)                                                                                                                                    aaCAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCAt                                                                                                             <  7:260198/58‑1 (MQ=255)                                                                                                                                    aaCAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCAt                                                                                                             >  8:260198/1‑58 (MQ=255)                                                                                                                                    aaCAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGCGTTTTTCCAGGTCGCGTACGCGCTCGTCGAGGTCTTCGGCAc                   <  7:267343/148‑1 (MQ=255)                                                                                                                                       aGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGCGTTTTTCCAGGTCGCGTACGCGCTCGTCGAGGTCTTCGGCACTGaa               <  6:360494/149‑1 (MQ=255)                                                                                                                                       aGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGCGTTTTTCCAGGTCGCGTACGCGCTCGTCGAGGTCTTCGGCACTGaa               >  5:110565/1‑149 (MQ=255)                                                                                                                                         tcGCAGCAGGACGTCGACCGCCTGATGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGCGTTTTTCCAGGTCGCGTAc                                          >  3:127418/2‑120 (MQ=255)                                                                                                                                         tcGCAGCAGGACGTCGACCGCCTGATGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGCGTTTTTCCAGGTCGCGTAc                                          <  4:127418/119‑1 (MQ=255)                                                                                                                                         tcGCAGCAGGACGTCGACCGCCTGATGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGCGTTTTTCCAGGTCGCGTACGCGCTCGTCGAGGTCTTCGGCACTGAACt             >  7:222999/2‑149 (MQ=255)                                                                                                                                         aCGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGCGTTTTTCCAGGTCGCGTACGCGCTCGTCGAGGTCTTCGGCACTGAACt             >  8:162741/1‑149 (MQ=255)                                                                                                                                         aCGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGCGTTTTTCCAGGTCGCGTACGCGCTCGTCGAGGTCTTCGGCACTGAACt             >  1:185351/1‑149 (MQ=255)                                                                                                                                             aTCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGCGTTTTTCCAGGTCGCGTACGCGCTCGTCGAGGTCTTCGGCACTGAACTGCCg         >  3:230009/1‑149 (MQ=255)                                                                                                                                              tCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCCTGACCTGCTTCAGGCGTTTTTCCAGGTCGCGTACGCGCTCGTCGAGGTCTTCGGCACTGAACTGCCGt        >  3:235690/1‑149 (MQ=255)                                                                                                                                                  gCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGCGTTTTTCCAGGTCGCGTACGCGCTCGTCGAGGTCTTCGGCACTGAACTGCCGTGACt    >  4:353486/1‑149 (MQ=255)                                                                                                                                                   cGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGCGTTTTTCCAGGTCGCGTACGCGCTCGTCGAGGTCTTCGGCACTGAACTGCCGTGACTg   >  8:319826/1‑149 (MQ=255)                                                                                                                                                    ggTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGCGTTTTTCCAGGTCGCGTACGCGCTCGTCGAGGTCTTCGGCACTGAACTGCCGTGACTgg  <  2:69225/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                     CCAGGCCTGGGGCCTCGAAGCGTTCGCCCTTGAAGCCGTCGAGCACCGCTTCCAGCGATTTTACCCACAGGTCGCTGTCGTCCAGCTCGATACCGCGATCTCCCAGCGGCAGGCTGAACAGCGCGCCGTTGAACAGACGCATCAGGCGGTCGACGTCCTGCTGCGAGAACTCTTCGCGCAGGCGGGCATAGCTGTTGTTGTCAGCGTGATCCAGCTGCTGCTTGACCTGCTTCAGGCGTTTTTCCAGGTCGCGTACGCGCTCGTCGAGGTCTTCGGCACTGAACTGCCGTGACTGG  >  NC_002947/805937‑806232

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑